(Circular) (Six-base) MAPSORT of: pFN21AE6589.seq Check: 1375 from: 1 to: 5239 pFN21AE6589 5239 bp With 182 enzymes: SgfI * March 18, 2015 14:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3049 278 Size: 53 83 186 936 1513 2468 Fragments arranged by size: 2468 1513 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2582 1937 Size: 645 4594 Acc65I G'GTAC_C Cuts at: 2560 2560 Size: 5239 AclI AA'CG_TT Cuts at: 1563 3366 3739 1563 Size: 1803 373 3063 Fragments arranged by size: 3063 1803 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3302 4686 4954 1632 Size: 1670 1384 268 1917 Fragments arranged by size: 1917 1670 1384 268 AfeI AGC'GCT Cuts at: 2372 2372 Size: 5239 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5042 AflIII A'CryG_T Cuts at: 1203 2200 2424 4138 5115 1203 Size: 997 224 1714 977 1327 Fragments arranged by size: 1714 1327 997 977 224 AgeI A'CCGG_T Cuts at: 4070 4070 Size: 5239 AhdI GACnn_n'nnGTC Cuts at: 3968 3968 Size: 5239 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5239 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5239 AlwNI CAG_nnn'CTG Cuts at: 2982 4554 2982 Size: 1572 3667 ApaLI G'TGCA_C Cuts at: 2048 3296 4452 2048 Size: 1248 1156 2835 Fragments arranged by size: 2835 1248 1156 ApoI r'AATT_y Cuts at: 1493 2228 2548 2774 2926 5035 1493 Size: 735 320 226 152 2109 1697 Fragments arranged by size: 2109 1697 735 320 226 152 AseI AT'TA_AT Cuts at: 160 1032 3793 160 Size: 872 2761 1606 Fragments arranged by size: 2761 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5239 AvaI C'yCGr_G Cuts at: 1870 1943 2014 2280 2564 1870 Size: 73 71 266 284 4545 Fragments arranged by size: 4545 284 266 73 71 AvrII C'CTAG_G Cuts at: 2131 2131 Size: 5239 BamHI G'GATC_C Cuts at: 2569 2936 2569 Size: 367 4872 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2497 2560 4015 618 Size: 325 217 627 710 63 1455 1842 Fragments arranged by size: 1842 1455 710 627 325 217 63 BanII G_rGCy'C Cuts at: 1391 2350 2558 1391 Size: 959 208 4072 Fragments arranged by size: 4072 959 208 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2259 928 Size: 971 360 3908 Fragments arranged by size: 3908 971 360 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3430 3464 1903 Size: 34 1493 34 3678 Fragments arranged by size: 3678 1493 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2152 3131 4341 2152 Size: 979 1210 3050 Fragments arranged by size: 3050 1210 979 BclI T'GATC_A Cuts at: 1548 1548 Size: 5239 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3850 136 Size: 107 122 71 631 605 2178 1525 Fragments arranged by size: 2178 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5234 5234 Size: 5239 BlpI GC'TnA_GC Cuts at: 2656 2656 Size: 5239 Bme1580I G_kGCm'C Cuts at: 1790 2052 2432 3300 4456 1790 Size: 262 380 868 1156 2573 Fragments arranged by size: 2573 1156 868 380 262 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5239 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2235 2970 3928 Size: 416 53 83 399 24 818 735 958 Cuts at: 3928 442 Size: 1753 Fragments arranged by size: 1753 958 818 735 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5239 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3899 1239 Size: 2660 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3364 4229 4527 3364 Size: 865 298 4076 Fragments arranged by size: 4076 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2121 3902 882 Size: 617 622 1781 2219 Fragments arranged by size: 2219 1781 622 617 BsaAI yAC'GTr Cuts at: 493 1204 2201 2427 3030 493 Size: 711 997 226 603 2702 Fragments arranged by size: 2702 997 711 603 226 BsaBI GATnn'nnATC Cuts at: 5233 5233 Size: 5239 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3046 3428 4063 275 Size: 53 83 186 936 1513 382 635 1451 Fragments arranged by size: 1513 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2252 3672 4070 4344 4491 4902 2252 Size: 1420 398 274 147 411 2589 Fragments arranged by size: 2589 1420 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2185 2215 1178 Size: 30 165 30 782 30 4202 Fragments arranged by size: 4202 782 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2139 2495 1186 Size: 953 356 3930 Fragments arranged by size: 3930 953 356 BseYI C'CCAG_C Cuts at: 2105 4442 4938 2105 Size: 2337 496 2406 Fragments arranged by size: 2406 2337 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2320 1163 Size: 573 584 4082 Fragments arranged by size: 4082 584 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2041 2689 3450 4478 1540 Size: 275 186 40 648 761 1028 2301 Fragments arranged by size: 2301 1028 761 648 275 186 40 BsiHKAI G_wGCw'C Cuts at: 2052 2290 3300 3385 4456 2052 Size: 238 1010 85 1071 2835 Fragments arranged by size: 2835 1071 1010 238 85 BsmI GAATG_Cn' Cuts at: 2750 2843 2750 Size: 93 5146 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2052 2290 2350 2432 2558 3300 3385 Size: 399 262 238 60 82 126 742 85 Cuts at: 3385 4456 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 262 238 126 85 82 60 BspEI T'CCGG_A Cuts at: 2252 4902 2252 Size: 2650 2589 BspHI T'CATG_A Cuts at: 3128 4858 3128 Size: 1730 3509 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2594 844 Size: 1750 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3126 1394 Size: 429 1303 3507 Fragments arranged by size: 3507 1303 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3734 3908 65 Size: 1183 2486 174 1396 Fragments arranged by size: 2486 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3883 4070 1700 Size: 110 2073 187 2869 Fragments arranged by size: 2869 2073 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5239 BssHII G'CGCG_C Cuts at: 1925 2030 1925 Size: 105 5134 BssSI C'ACGA_G Cuts at: 3299 4311 3299 Size: 1012 4227 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5239 BstBI TT'CG_AA Cuts at: 4043 4043 Size: 5239 BstEII G'GTnAC_C Cuts at: 1184 2060 1184 Size: 876 4363 BstYI r'GATC_y Cuts at: 1972 2569 2936 3321 3338 4790 4876 4888 5234 Size: 597 367 385 17 1452 86 12 346 Cuts at: 5234 1972 Size: 1977 Fragments arranged by size: 1977 1452 597 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2331 513 Size: 552 939 327 3421 Fragments arranged by size: 3421 939 552 327 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4500 Fragments arranged by size: 4500 683 56 BtsI GCAGTG_nn' Cuts at: 2755 3549 3569 2755 Size: 794 20 4425 Fragments arranged by size: 4425 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5239 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5204 DraI TTT'AAA Cuts at: 2543 2543 Size: 5239 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4246 809 Size: 507 2930 1802 Fragments arranged by size: 2930 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2038 2686 3575 Size: 54 1425 50 275 18 208 648 889 Cuts at: 3575 5204 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 648 275 208 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2038 2686 1537 Size: 275 226 648 4090 Fragments arranged by size: 4090 648 275 226 EarI CTCTTCn'nnn_ Cuts at: 1353 3169 1353 Size: 1816 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2351 3826 4200 4346 387 Size: 1064 138 762 1475 374 146 1280 Fragments arranged by size: 1475 1280 1064 762 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3302 3899 4686 4954 1239 Size: 393 1670 597 787 268 1524 Fragments arranged by size: 1670 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2299 2363 2299 Size: 64 5175 EcoRI G'AATT_C Cuts at: 2548 2926 2548 Size: 378 4861 FspI TGC'GCA Cuts at: 3745 3745 Size: 5239 HaeII r_GCGC'y Cuts at: 1678 2374 2389 4386 1678 Size: 696 15 1997 2531 Fragments arranged by size: 2531 1997 696 15 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3880 3912 3954 3986 1373 Size: 32 135 32 2308 32 42 32 2626 Fragments arranged by size: 2626 2308 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2583 2829 669 Size: 988 281 645 246 3079 Fragments arranged by size: 3079 988 645 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5239 HpaI GTT'AAC Cuts at: 2829 2829 Size: 5239 KpnI G_GTAC'C Cuts at: 2564 2564 Size: 5239 MfeI C'AATT_G Cuts at: 2838 2838 Size: 5239 MluI A'CGCG_T Cuts at: 5115 5115 Size: 5239 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2155 4353 4537 1305 Size: 850 2198 184 2007 Fragments arranged by size: 2198 2007 850 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3417 Fragments arranged by size: 3417 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3197 3556 3715 518 Size: 648 2031 359 159 2042 Fragments arranged by size: 2042 2031 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2308 2333 2655 3332 4480 4725 1606 Size: 702 25 322 677 1148 245 2120 Fragments arranged by size: 2120 1148 702 677 322 245 25 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3748 Fragments arranged by size: 3748 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5239 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5239 NotI GC'GGCC_GC Cuts at: 2686 2686 Size: 5239 NspI r_CATG'y Cuts at: 1132 1229 2597 4142 1132 Size: 97 1368 1545 2229 Fragments arranged by size: 2229 1545 1368 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5239 PciI A'CATG_T Cuts at: 4138 4138 Size: 5239 PflMI CCAn_nnn'nTGG Cuts at: 1108 2112 1108 Size: 1004 4235 PmeI GTTT'AAAC Cuts at: 2543 2543 Size: 5239 PmlI CAC'GTG Cuts at: 2201 2427 2201 Size: 226 5013 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3308 3340 4821 4853 3308 Size: 32 1481 32 3694 Fragments arranged by size: 3694 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5239 PsiI TTA'TAA Cuts at: 2809 2809 Size: 5239 PstI C_TGCA'G Cuts at: 830 2103 2591 830 Size: 1273 488 3478 Fragments arranged by size: 3478 1273 488 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5239 RsrII CG'GwC_CG Cuts at: 2043 2043 Size: 5239 SacII CC_GC'GG Cuts at: 2334 2334 Size: 5239 SalI G'TCGA_C Cuts at: 1936 2581 1936 Size: 645 4594 SbfI CC_TGCA'GG Cuts at: 2591 2591 Size: 5239 ScaI AGT'ACT Cuts at: 3487 5199 3487 Size: 1712 3527 SfcI C'TryA_G Cuts at: 826 1045 2099 2587 3722 4403 4594 826 Size: 219 1054 488 1135 681 191 1471 Fragments arranged by size: 1471 1135 1054 681 488 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5239 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5239 SmaI CCC'GGG Cuts at: 1872 2016 2566 1872 Size: 144 550 4545 Fragments arranged by size: 4545 550 144 SmlI C'TyrA_G Cuts at: 820 1017 1943 3343 4244 4506 820 Size: 197 926 1400 901 262 1553 Fragments arranged by size: 1553 1400 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3030 493 Size: 2537 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5239 SphI G_CATG'C Cuts at: 1132 2597 1132 Size: 1465 3774 SspI AAT'ATT Cuts at: 5 52 3163 5 Size: 47 3111 2081 Fragments arranged by size: 3111 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5239 StyI C'CwwG_G Cuts at: 513 1065 2004 2131 2678 513 Size: 552 939 127 547 3074 Fragments arranged by size: 3074 939 552 547 127 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2427 3266 3283 3436 3621 4066 325 Size: 1255 847 839 17 153 185 445 1498 Fragments arranged by size: 1498 1255 847 839 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3485 5197 96 Size: 274 80 33 51 1443 1508 1712 138 Fragments arranged by size: 1712 1508 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2185 2217 5199 5231 2185 Size: 32 2982 32 2193 Fragments arranged by size: 2982 2193 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5239 XbaI T'CTAG_A Cuts at: 2575 2575 Size: 5239 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5239 XmaI C'CCGG_G Cuts at: 1870 2014 2564 1870 Size: 144 550 4545 Fragments arranged by size: 4545 550 144 XmnI GAAnn'nnTTC Cuts at: 3368 3368 Size: 5239 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3047 276 Size: 53 83 186 936 1513 2468 Fragments arranged by size: 2468 1513 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI RsrII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI PvuII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI