(Linear) (Six-base) MAPSORT of: KIEE6589.seq Check: 3925 from: 1 to: 534 KIEE6589.orf With 182 enzymes: SgfI * March 16, 2015 11:34 .. AfeI AGC'GCT Cuts at: 0 367 534 Size: 367 167 AflIII A'CryG_T Cuts at: 0 195 419 534 Size: 195 224 115 Fragments arranged by size: 224 195 115 ApaLI G'TGCA_C Cuts at: 0 43 534 Size: 43 491 ApoI r'AATT_y Cuts at: 0 223 534 Size: 223 311 AvaI C'yCGr_G Cuts at: 0 9 275 534 Size: 9 266 259 Fragments arranged by size: 266 259 9 AvrII C'CTAG_G Cuts at: 0 126 534 Size: 126 408 BanI G'GyrC_C Cuts at: 0 492 534 Size: 492 42 BanII G_rGCy'C Cuts at: 0 345 534 Size: 345 189 BbsI GAAGACnn'nnnn_ Cuts at: 0 254 534 Size: 254 280 BciVI GTATCCnnnnn_n' Cuts at: 0 147 534 Size: 147 387 Bme1580I G_kGCm'C Cuts at: 0 47 427 534 Size: 47 380 107 Fragments arranged by size: 380 107 47 BmrI ACTGGGnnnn_n' Cuts at: 0 230 534 Size: 230 304 BsaI GGTCTCn'nnnn_ Cuts at: 0 116 534 Size: 116 418 BsaAI yAC'GTr Cuts at: 0 196 422 534 Size: 196 226 112 Fragments arranged by size: 226 196 112 BsaWI w'CCGG_w Cuts at: 0 247 534 Size: 247 287 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 180 210 534 Size: 180 30 324 Fragments arranged by size: 324 180 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 134 490 534 Size: 134 356 44 Fragments arranged by size: 356 134 44 BseYI C'CCAG_C Cuts at: 0 100 534 Size: 100 434 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 315 534 Size: 315 219 BsiEI CG_ry'CG Cuts at: 0 36 534 Size: 36 498 BsiHKAI G_wGCw'C Cuts at: 0 47 285 534 Size: 47 238 249 Fragments arranged by size: 249 238 47 Bsp1286I G_dGCh'C Cuts at: 0 47 285 345 427 534 Size: 47 238 60 82 107 Fragments arranged by size: 238 107 82 60 47 BspEI T'CCGG_A Cuts at: 0 247 534 Size: 247 287 BssHII G'CGCG_C Cuts at: 0 25 534 Size: 25 509 BstEII G'GTnAC_C Cuts at: 0 55 534 Size: 55 479 BtgI C'CryG_G Cuts at: 0 326 534 Size: 326 208 EaeI y'GGCC_r Cuts at: 0 33 534 Size: 33 501 EagI C'GGCC_G Cuts at: 0 33 534 Size: 33 501 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 346 534 Size: 346 188 EcoNI CCTnn'n_nnAGG Cuts at: 0 294 358 534 Size: 294 64 176 Fragments arranged by size: 294 176 64 HaeII r_GCGC'y Cuts at: 0 369 384 534 Size: 369 15 150 Fragments arranged by size: 369 150 15 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 150 534 Size: 150 384 MspA1I CmG'CkG Cuts at: 0 303 328 534 Size: 303 25 206 Fragments arranged by size: 303 206 25 PflMI CCAn_nnn'nTGG Cuts at: 0 107 534 Size: 107 427 PmlI CAC'GTG Cuts at: 0 196 422 534 Size: 196 226 112 Fragments arranged by size: 226 196 112 PstI C_TGCA'G Cuts at: 0 98 534 Size: 98 436 RsrII CG'GwC_CG Cuts at: 0 38 534 Size: 38 496 SacII CC_GC'GG Cuts at: 0 329 534 Size: 329 205 SfcI C'TryA_G Cuts at: 0 94 534 Size: 94 440 SmaI CCC'GGG Cuts at: 0 11 534 Size: 11 523 StyI C'CwwG_G Cuts at: 0 126 534 Size: 126 408 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 422 534 Size: 422 112 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 180 212 534 Size: 180 32 322 Fragments arranged by size: 322 180 32 XmaI C'CCGG_G Cuts at: 0 9 534 Size: 9 525 Enzymes that do cut: AfeI AflIII ApaLI ApoI AvaI AvrII BanI BanII BbsI BciVI Bme1580I BmrI BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BssHII BstEII BtgI EaeI EagI EciI EcoNI HaeII MmeI MspA1I PflMI PmlI PstI RsrII SacII SfcI SmaI StyI TaqII TstI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AflII AgeI AhdI AleI AlfI AloI AlwNI ApaI AscI AseI AsiSI BaeI BamHI BbeI BbvCI BcgI BclI BfrBI BglI BglII BlpI BmgBI BmtI BplI BpmI Bpu10I BpuEI BsaBI BsaHI BsiWI BsmI BsmBI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EarI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MscI MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI Tth111I XbaI XcmI XhoI XmnI ZraI