(Circular) (Six-base) MAPSORT of: pFN21AE3950.seq Check: 6933 from: 1 to: 7054 pFN21AE3950 7054 bp With 182 enzymes: SgfI * March 28, 2012 11:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2145 3905 2145 Size: 1760 5294 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2603 2688 4864 278 Size: 53 83 186 936 1067 85 2176 2468 Fragments arranged by size: 2468 2176 1067 936 186 85 83 53 AccI GT'mk_AC Cuts at: 1937 4397 1937 Size: 2460 4594 Acc65I G'GTAC_C Cuts at: 2568 4375 2568 Size: 1807 5247 AclI AA'CG_TT Cuts at: 1563 5181 5554 1563 Size: 3618 373 3063 Fragments arranged by size: 3618 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2039 4219 5117 6501 6769 1632 Size: 407 2180 898 1384 268 1917 Fragments arranged by size: 2180 1917 1384 898 407 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6857 AflIII A'CryG_T Cuts at: 1203 2909 5953 6930 1203 Size: 1706 3044 977 1327 Fragments arranged by size: 3044 1706 1327 977 AgeI A'CCGG_T Cuts at: 3978 5885 3978 Size: 1907 5147 AhdI GACnn_n'nnGTC Cuts at: 5783 5783 Size: 7054 AleI CACnn'nnGTG Cuts at: 1166 3236 1166 Size: 2070 4984 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2232 2561 1184 Size: 1048 329 5677 Fragments arranged by size: 5677 1048 329 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3347 3379 3347 Size: 32 7022 AlwNI CAG_nnn'CTG Cuts at: 2137 4797 6369 2137 Size: 2660 1572 2822 Fragments arranged by size: 2822 2660 1572 ApaI G_GGCC'C Cuts at: 2805 4226 2805 Size: 1421 5633 ApaLI G'TGCA_C Cuts at: 3953 5111 6267 3953 Size: 1158 1156 4740 Fragments arranged by size: 4740 1158 1156 ApoI r'AATT_y Cuts at: 1493 4363 4589 4741 6850 1493 Size: 2870 226 152 2109 1697 Fragments arranged by size: 2870 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5608 160 Size: 872 4576 1606 Fragments arranged by size: 4576 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7054 AvaI C'yCGr_G Cuts at: 1870 1943 2160 2353 2430 2549 2727 2854 2915 Size: 73 217 193 77 119 178 127 61 Cuts at: 2915 3753 4379 1870 Size: 838 626 4545 Fragments arranged by size: 4545 838 626 217 193 178 127 119 77 73 61 BamHI G'GATC_C Cuts at: 4384 4751 4384 Size: 367 6687 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2183 2395 2435 2568 2810 Size: 325 217 627 396 212 40 133 242 Cuts at: 2810 2822 3434 3538 3665 4375 5830 618 Size: 12 612 104 127 710 1455 1842 Fragments arranged by size: 1842 1455 710 627 612 396 325 242 217 212 133 127 104 40 12 BanII G_rGCy'C Cuts at: 1391 2471 2805 2855 4226 4373 1391 Size: 1080 334 50 1371 147 4072 Fragments arranged by size: 4072 1371 1080 334 147 50 BbeI G_GCGC'C Cuts at: 2187 2826 3438 2187 Size: 639 612 5803 Fragments arranged by size: 5803 639 612 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2454 3719 4246 928 Size: 971 555 1265 527 3736 Fragments arranged by size: 3736 1265 971 555 527 BbvCI CC'TCA_GC Cuts at: 4268 4268 Size: 7054 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5245 5279 1903 Size: 34 3308 34 3678 Fragments arranged by size: 3678 3308 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4946 6156 4946 Size: 1210 5844 BclI T'GATC_A Cuts at: 1548 3345 3393 1548 Size: 1797 48 5209 Fragments arranged by size: 5209 1797 48 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2271 2526 2859 Size: 107 122 71 631 605 599 255 333 Cuts at: 2859 5665 136 Size: 2806 1525 Fragments arranged by size: 2806 1525 631 605 599 333 255 122 107 71 BglII A'GATC_T Cuts at: 3378 7049 3378 Size: 3671 3383 BlpI GC'TnA_GC Cuts at: 2309 4471 2309 Size: 2162 4892 Bme1580I G_kGCm'C Cuts at: 1790 2400 2483 2805 3932 3957 4226 5115 6271 Size: 610 83 322 1127 25 269 889 1156 Cuts at: 6271 1790 Size: 2573 Fragments arranged by size: 2573 1156 1127 889 610 322 269 83 25 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7054 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2889 3970 4785 Size: 416 53 83 399 24 1472 1081 815 Cuts at: 4785 5743 442 Size: 958 1753 Fragments arranged by size: 1753 1472 1081 958 815 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7054 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2812 2812 Size: 7054 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2225 3005 3359 3461 3584 3625 3671 5714 Size: 986 780 354 102 123 41 46 2043 Cuts at: 5714 1239 Size: 2579 Fragments arranged by size: 2579 2043 986 780 354 123 102 46 41 Bpu10I CC'TnA_GC Cuts at: 4268 4268 Size: 7054 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2345 2696 5179 6044 6342 2345 Size: 351 2483 865 298 3057 Fragments arranged by size: 3057 2483 865 351 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2286 5717 882 Size: 617 787 3431 2219 Fragments arranged by size: 3431 2219 787 617 BsaAI yAC'GTr Cuts at: 493 1204 2478 2910 3973 4243 4845 493 Size: 711 1274 432 1063 270 602 2702 Fragments arranged by size: 2702 1274 1063 711 602 432 270 BsaBI GATnn'nnATC Cuts at: 7048 7048 Size: 7054 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2184 2600 2685 2823 Size: 53 83 186 936 651 416 85 138 Cuts at: 2823 3435 4861 5243 5878 275 Size: 612 1426 382 635 1451 Fragments arranged by size: 1451 1426 936 651 635 612 416 382 186 138 85 83 53 BsaWI w'CCGG_w Cuts at: 3317 3978 5487 5885 6159 6306 6717 3317 Size: 661 1509 398 274 147 411 3654 Fragments arranged by size: 3654 1509 661 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3347 3377 3695 3725 4097 Size: 30 165 30 1944 30 318 30 372 Cuts at: 4097 4127 1178 Size: 30 4105 Fragments arranged by size: 4105 1944 372 318 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2404 2437 3002 1186 Size: 1218 33 565 5238 Fragments arranged by size: 5238 1218 565 33 BseYI C'CCAG_C Cuts at: 2142 2828 3022 3925 4045 6257 6753 2142 Size: 686 194 903 120 2212 496 2443 Fragments arranged by size: 2443 2212 903 686 496 194 120 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2132 2262 2274 2582 2777 3277 3559 Size: 573 396 130 12 308 195 500 282 Cuts at: 3559 3953 4283 4307 1163 Size: 394 330 24 3910 Fragments arranged by size: 3910 573 500 396 394 330 308 282 195 130 24 12 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2641 3916 4504 5265 6293 1540 Size: 275 186 640 1275 588 761 1028 2301 Fragments arranged by size: 2301 1275 1028 761 640 588 275 186 BsiHKAI G_wGCw'C Cuts at: 2211 2471 3957 5115 5200 6271 2211 Size: 260 1486 1158 85 1071 2994 Fragments arranged by size: 2994 1486 1158 1071 260 85 BsmI GAATG_Cn' Cuts at: 4565 4658 4565 Size: 93 6961 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2211 2400 2471 2483 2805 2855 3932 Size: 399 421 189 71 12 322 50 1077 Cuts at: 3932 3957 4226 4373 5115 5200 6271 1391 Size: 25 269 147 742 85 1071 2174 Fragments arranged by size: 2174 1077 1071 742 421 399 322 269 189 147 85 71 50 25 12 BspEI T'CCGG_A Cuts at: 6717 6717 Size: 7054 BspHI T'CATG_A Cuts at: 4943 6673 4943 Size: 1730 5324 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2145 3905 4279 4409 844 Size: 1301 1760 374 130 3489 Fragments arranged by size: 3489 1760 1301 374 130 BsrBI CCG'CTC Cuts at: 1394 1823 2149 4941 1394 Size: 429 326 2792 3507 Fragments arranged by size: 3507 2792 429 326 BsrDI GCAATG_nn' Cuts at: 65 1248 4357 5549 5723 65 Size: 1183 3109 1192 174 1396 Fragments arranged by size: 3109 1396 1192 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2541 2994 3978 5698 5885 1700 Size: 110 731 453 984 1720 187 2869 Fragments arranged by size: 2869 1720 984 731 453 187 110 BsrGI T'GTAC_A Cuts at: 96 2316 96 Size: 2220 4834 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7054 BssSI C'ACGA_G Cuts at: 3008 5114 6126 3008 Size: 2106 1012 3936 Fragments arranged by size: 3936 2106 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2137 2946 794 Size: 1343 809 4902 Fragments arranged by size: 4902 1343 809 BstBI TT'CG_AA Cuts at: 5858 5858 Size: 7054 BstEII G'GTnAC_C Cuts at: 1184 4291 1184 Size: 3107 3947 BstXI CCAn_nnnn'nTGG Cuts at: 4078 4078 Size: 7054 BstYI r'GATC_y Cuts at: 1972 3138 3378 4384 4751 5136 5153 6605 6691 Size: 1166 240 1006 367 385 17 1452 86 Cuts at: 6691 6703 7049 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1166 1006 385 367 346 240 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2391 2515 2581 2610 3633 4158 Size: 552 939 387 124 66 29 1023 525 Cuts at: 4158 513 Size: 3409 Fragments arranged by size: 3409 1023 939 552 525 387 124 66 29 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2089 2732 487 Size: 683 56 863 643 4809 Fragments arranged by size: 4809 863 683 643 56 BtsI GCAGTG_nn' Cuts at: 2492 2684 3289 3428 4105 4570 5364 5384 2492 Size: 192 605 139 677 465 794 20 4162 Fragments arranged by size: 4162 794 677 605 465 192 139 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7054 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7019 DraI TTT'AAA Cuts at: 4358 4358 Size: 7054 DraIII CAC_nnn'GTG Cuts at: 3013 3013 Size: 7054 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6061 809 Size: 507 4745 1802 Fragments arranged by size: 4745 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2619 4014 4334 Size: 54 1425 50 275 18 789 1395 320 Cuts at: 4334 4501 5390 7019 8 Size: 167 889 1629 43 Fragments arranged by size: 1629 1425 1395 889 789 320 275 167 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4501 1537 Size: 275 2689 4090 Fragments arranged by size: 4090 2689 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2007 3778 4984 1353 Size: 654 1771 1206 3423 Fragments arranged by size: 3423 1771 1206 654 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2304 5641 6015 6161 387 Size: 1064 138 715 3337 374 146 1280 Fragments arranged by size: 3337 1280 1064 715 374 146 138 EcoICRI GAG'CTC Cuts at: 2469 2469 Size: 7054 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2039 2225 3005 3359 3461 3584 3625 Size: 393 407 186 780 354 102 123 41 Cuts at: 3625 3671 4219 5117 5714 6501 6769 1239 Size: 46 548 898 597 787 268 1524 Fragments arranged by size: 1524 898 787 780 597 548 407 393 354 268 186 123 102 46 41 EcoNI CCTnn'n_nnAGG Cuts at: 2987 2987 Size: 7054 EcoO109I rG'GnC_Cy Cuts at: 2343 2732 4222 4223 2343 Size: 389 1490 1 5174 Fragments arranged by size: 5174 1490 389 1 EcoRI G'AATT_C Cuts at: 4363 4741 4363 Size: 378 6676 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2329 2329 Size: 7054 FspI TGC'GCA Cuts at: 5560 5560 Size: 7054 HaeII r_GCGC'y Cuts at: 1678 2187 2826 3438 6201 1678 Size: 509 639 612 2763 2531 Fragments arranged by size: 2763 2531 639 612 509 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3833 3865 5695 5727 5769 Size: 32 135 32 2261 32 1830 32 42 Cuts at: 5769 5801 1373 Size: 32 2626 Fragments arranged by size: 2626 2261 1830 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4398 4644 669 Size: 988 281 2460 246 3079 Fragments arranged by size: 3079 2460 988 281 246 HindIII A'AGCT_T Cuts at: 748 3762 748 Size: 3014 4040 HpaI GTT'AAC Cuts at: 4644 4644 Size: 7054 KasI G'GCGC_C Cuts at: 2183 2822 3434 2183 Size: 639 612 5803 Fragments arranged by size: 5803 639 612 KpnI G_GTAC'C Cuts at: 2572 4379 2572 Size: 1807 5247 MfeI C'AATT_G Cuts at: 4653 4653 Size: 7054 MluI A'CGCG_T Cuts at: 6930 6930 Size: 7054 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6168 6352 1305 Size: 4863 184 2007 Fragments arranged by size: 4863 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2621 4016 10 Size: 54 1425 343 789 1395 3048 Fragments arranged by size: 3048 1425 1395 789 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2108 3236 3524 4076 5012 5371 5530 Size: 648 942 1128 288 552 936 359 159 Cuts at: 5530 518 Size: 2042 Fragments arranged by size: 2042 1128 942 936 648 552 359 288 159 MspA1I CmG'CkG Cuts at: 1606 2195 2258 2334 2517 2566 2583 2612 2632 Size: 589 63 76 183 49 17 29 20 Cuts at: 2632 2832 3022 3490 3511 3562 3935 4095 4470 Size: 200 190 468 21 51 373 160 375 Cuts at: 4470 5147 6295 6540 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 589 468 375 373 245 200 190 183 160 76 63 51 49 29 21 20 17 NaeI GCC'GGC Cuts at: 2996 2996 Size: 7054 NarI GG'CG_CC Cuts at: 2184 2823 3435 2184 Size: 639 612 5803 Fragments arranged by size: 5803 639 612 NcoI C'CATG_G Cuts at: 513 1065 2004 2391 513 Size: 552 939 387 5176 Fragments arranged by size: 5176 939 552 387 NdeI CA'TA_TG Cuts at: 387 387 Size: 7054 NgoMIV G'CCGG_C Cuts at: 2994 2994 Size: 7054 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7054 NotI GC'GGCC_GC Cuts at: 4501 4501 Size: 7054 NspI r_CATG'y Cuts at: 1132 1229 2944 4412 5957 1132 Size: 97 1715 1468 1545 2229 Fragments arranged by size: 2229 1715 1545 1468 97 PasI CC'CwG_GG Cuts at: 1348 2818 1348 Size: 1470 5584 PciI A'CATG_T Cuts at: 5953 5953 Size: 7054 PflMI CCAn_nnn'nTGG Cuts at: 1108 3511 1108 Size: 2403 4651 PfoI T'CCnGG_A Cuts at: 3141 3648 3681 3141 Size: 507 33 6514 Fragments arranged by size: 6514 507 33 PmeI GTTT'AAAC Cuts at: 4358 4358 Size: 7054 PmlI CAC'GTG Cuts at: 2478 2910 3973 4243 2478 Size: 432 1063 270 5289 Fragments arranged by size: 5289 1063 432 270 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3347 3379 5123 5155 6636 6668 3347 Size: 32 1744 32 1481 32 3733 Fragments arranged by size: 3733 1744 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2343 2732 2343 Size: 389 6665 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7054 PsiI TTA'TAA Cuts at: 4624 4624 Size: 7054 PspOMI G'GGCC_C Cuts at: 2801 4222 2801 Size: 1421 5633 PspXI vC'TCGA_Gb Cuts at: 2549 2549 Size: 7054 PstI C_TGCA'G Cuts at: 830 2178 2608 3498 3561 3813 3902 4406 830 Size: 1348 430 890 63 252 89 504 3478 Fragments arranged by size: 3478 1348 890 504 430 252 89 63 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7054 PvuII CAG'CTG Cuts at: 2258 2632 3511 3562 3935 4095 2258 Size: 374 879 51 373 160 5217 Fragments arranged by size: 5217 879 374 373 160 51 SacI G_AGCT'C Cuts at: 2471 2471 Size: 7054 SacII CC_GC'GG Cuts at: 2518 2584 2613 2518 Size: 66 29 6959 Fragments arranged by size: 6959 66 29 SalI G'TCGA_C Cuts at: 1936 4396 1936 Size: 2460 4594 SanDI GG'GwC_CC Cuts at: 2732 2732 Size: 7054 SapI GCTCTTCn'nnn_ Cuts at: 2007 2007 Size: 7054 SbfI CC_TGCA'GG Cuts at: 4406 4406 Size: 7054 ScaI AGT'ACT Cuts at: 3332 5302 7014 3332 Size: 1970 1712 3372 Fragments arranged by size: 3372 1970 1712 SfcI C'TryA_G Cuts at: 826 1045 2174 2604 2935 3494 3557 3809 3898 Size: 219 1129 430 331 559 63 252 89 Cuts at: 3898 4402 5537 6218 6409 826 Size: 504 1135 681 191 1471 Fragments arranged by size: 1471 1135 1129 681 559 504 430 331 252 219 191 89 63 SfoI GGC'GCC Cuts at: 2185 2824 3436 2185 Size: 639 612 5803 Fragments arranged by size: 5803 639 612 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7054 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7054 SmaI CCC'GGG Cuts at: 1872 2162 2729 2856 4381 1872 Size: 290 567 127 1525 4545 Fragments arranged by size: 4545 1525 567 290 127 SmlI C'TyrA_G Cuts at: 820 1017 1943 2360 2549 2711 5158 6059 6321 Size: 197 926 417 189 162 2447 901 262 Cuts at: 6321 820 Size: 1553 Fragments arranged by size: 2447 1553 926 901 417 262 197 189 162 SnaBI TAC'GTA Cuts at: 493 4845 493 Size: 4352 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7054 SphI G_CATG'C Cuts at: 1132 4412 1132 Size: 3280 3774 SspI AAT'ATT Cuts at: 5 52 4978 5 Size: 47 4926 2081 Fragments arranged by size: 4926 2081 47 StuI AGG'CCT Cuts at: 1517 3065 4064 1517 Size: 1548 999 4507 Fragments arranged by size: 4507 1548 999 StyI C'CwwG_G Cuts at: 513 1065 2004 2391 3234 3945 4493 513 Size: 552 939 387 843 711 548 3074 Fragments arranged by size: 3074 939 843 711 552 548 387 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3638 4008 5081 5098 5251 5436 5881 Size: 1255 2058 370 1073 17 153 185 445 Cuts at: 5881 325 Size: 1498 Fragments arranged by size: 2058 1498 1255 1073 445 370 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2316 2943 3330 Size: 274 80 33 51 1443 339 627 387 Cuts at: 3330 5300 7012 96 Size: 1970 1712 138 Fragments arranged by size: 1970 1712 1443 627 387 339 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3695 3727 4097 4120 4129 4152 4299 4331 7014 Size: 32 370 23 9 23 147 32 2683 Cuts at: 7014 7046 3695 Size: 32 3703 Fragments arranged by size: 3703 2683 370 147 32 32 32 23 23 9 Tth111I GACn'n_nGTC Cuts at: 1316 4250 1316 Size: 2934 4120 XbaI T'CTAG_A Cuts at: 4390 4390 Size: 7054 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2749 3322 3508 4024 2749 Size: 573 186 516 5779 Fragments arranged by size: 5779 573 516 186 XhoI C'TCGA_G Cuts at: 1943 2549 1943 Size: 606 6448 XmaI C'CCGG_G Cuts at: 1870 2160 2727 2854 4379 1870 Size: 290 567 127 1525 4545 Fragments arranged by size: 4545 1525 567 290 127 XmnI GAAnn'nnTTC Cuts at: 5183 5183 Size: 7054 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2601 2686 4862 276 Size: 53 83 186 936 1067 85 2176 2468 Fragments arranged by size: 2468 2176 1067 936 186 85 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI FspAI NruI NsiI PacI PsrI RsrII SexAI SfiI SrfI SwaI