(Linear) (Six-base) MAPSORT of: KIEE3950.seq Check: 4112 from: 1 to: 2349 KIEE3950.orf With 182 enzymes: SgfI * March 22, 2012 16:57 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 140 1900 2349 Size: 140 1760 449 Fragments arranged by size: 1760 449 140 AatII G_ACGT'C Cuts at: 0 598 683 2349 Size: 598 85 1666 Fragments arranged by size: 1666 598 85 Acc65I G'GTAC_C Cuts at: 0 563 2349 Size: 563 1786 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 34 2214 2349 Size: 34 2180 135 Fragments arranged by size: 2180 135 34 AflIII A'CryG_T Cuts at: 0 904 2349 Size: 904 1445 AgeI A'CCGG_T Cuts at: 0 1973 2349 Size: 1973 376 AleI CACnn'nnGTG Cuts at: 0 1231 2349 Size: 1231 1118 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 227 556 2349 Size: 227 329 1793 Fragments arranged by size: 1793 329 227 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1342 1374 2349 Size: 1342 32 975 Fragments arranged by size: 1342 975 32 AlwNI CAG_nnn'CTG Cuts at: 0 132 2349 Size: 132 2217 ApaI G_GGCC'C Cuts at: 0 800 2221 2349 Size: 800 1421 128 Fragments arranged by size: 1421 800 128 ApaLI G'TGCA_C Cuts at: 0 1948 2349 Size: 1948 401 AvaI C'yCGr_G Cuts at: 0 155 348 425 544 722 849 910 1748 Size: 155 193 77 119 178 127 61 838 Cuts at: 1748 2349 Size: 601 Fragments arranged by size: 838 601 193 178 155 127 119 77 61 BanI G'GyrC_C Cuts at: 0 178 390 430 563 805 817 1429 1533 Size: 178 212 40 133 242 12 612 104 Cuts at: 1533 1660 2349 Size: 127 689 Fragments arranged by size: 689 612 242 212 178 133 127 104 40 12 BanII G_rGCy'C Cuts at: 0 466 800 850 2221 2349 Size: 466 334 50 1371 128 Fragments arranged by size: 1371 466 334 128 50 BbeI G_GCGC'C Cuts at: 0 182 821 1433 2349 Size: 182 639 612 916 Fragments arranged by size: 916 639 612 182 BbsI GAAGACnn'nnnn_ Cuts at: 0 449 1714 2241 2349 Size: 449 1265 527 108 Fragments arranged by size: 1265 527 449 108 BbvCI CC'TCA_GC Cuts at: 0 2263 2349 Size: 2263 86 BclI T'GATC_A Cuts at: 0 1340 1388 2349 Size: 1340 48 961 Fragments arranged by size: 1340 961 48 BglI GCCn_nnn'nGGC Cuts at: 0 266 521 854 2349 Size: 266 255 333 1495 Fragments arranged by size: 1495 333 266 255 BglII A'GATC_T Cuts at: 0 1373 2349 Size: 1373 976 BlpI GC'TnA_GC Cuts at: 0 304 2349 Size: 304 2045 Bme1580I G_kGCm'C Cuts at: 0 395 478 800 1927 1952 2221 2349 Size: 395 83 322 1127 25 269 128 Fragments arranged by size: 1127 395 322 269 128 83 25 BmrI ACTGGGnnnn_n' Cuts at: 0 884 1965 2349 Size: 884 1081 384 Fragments arranged by size: 1081 884 384 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 807 2349 Size: 807 1542 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 220 1000 1354 1456 1579 1620 1666 2349 Size: 220 780 354 102 123 41 46 683 Fragments arranged by size: 780 683 354 220 123 102 46 41 Bpu10I CC'TnA_GC Cuts at: 0 2263 2349 Size: 2263 86 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 340 691 2349 Size: 340 351 1658 Fragments arranged by size: 1658 351 340 BsaI GGTCTCn'nnnn_ Cuts at: 0 281 2349 Size: 281 2068 BsaAI yAC'GTr Cuts at: 0 473 905 1968 2238 2349 Size: 473 432 1063 270 111 Fragments arranged by size: 1063 473 432 270 111 BsaHI Gr'CG_yC Cuts at: 0 179 595 680 818 1430 2349 Size: 179 416 85 138 612 919 Fragments arranged by size: 919 612 416 179 138 85 BsaWI w'CCGG_w Cuts at: 0 1312 1973 2349 Size: 1312 661 376 Fragments arranged by size: 1312 661 376 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1342 1372 1690 1720 2092 2122 2349 Size: 1342 30 318 30 372 30 227 Fragments arranged by size: 1342 372 318 227 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 399 432 997 2349 Size: 399 33 565 1352 Fragments arranged by size: 1352 565 399 33 BseYI C'CCAG_C Cuts at: 0 137 823 1017 1920 2040 2349 Size: 137 686 194 903 120 309 Fragments arranged by size: 903 686 309 194 137 120 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 127 257 269 577 772 1272 1554 1948 Size: 127 130 12 308 195 500 282 394 Cuts at: 1948 2278 2302 2349 Size: 330 24 47 Fragments arranged by size: 500 394 330 308 282 195 130 127 47 24 12 BsiEI CG_ry'CG Cuts at: 0 636 1911 2349 Size: 636 1275 438 Fragments arranged by size: 1275 636 438 BsiHKAI G_wGCw'C Cuts at: 0 206 466 1952 2349 Size: 206 260 1486 397 Fragments arranged by size: 1486 397 260 206 Bsp1286I G_dGCh'C Cuts at: 0 206 395 466 478 800 850 1927 1952 Size: 206 189 71 12 322 50 1077 25 Cuts at: 1952 2221 2349 Size: 269 128 Fragments arranged by size: 1077 322 269 206 189 128 71 50 25 12 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 140 1900 2274 2349 Size: 140 1760 374 75 Fragments arranged by size: 1760 374 140 75 BsrBI CCG'CTC Cuts at: 0 144 2349 Size: 144 2205 BsrFI r'CCGG_y Cuts at: 0 536 989 1973 2349 Size: 536 453 984 376 Fragments arranged by size: 984 536 453 376 BsrGI T'GTAC_A Cuts at: 0 311 2349 Size: 311 2038 BssSI C'ACGA_G Cuts at: 0 1003 2349 Size: 1003 1346 BstAPI GCAn_nnn'nTGC Cuts at: 0 132 941 2349 Size: 132 809 1408 Fragments arranged by size: 1408 809 132 BstEII G'GTnAC_C Cuts at: 0 2286 2349 Size: 2286 63 BstXI CCAn_nnnn'nTGG Cuts at: 0 2073 2349 Size: 2073 276 BstYI r'GATC_y Cuts at: 0 1133 1373 2349 Size: 1133 240 976 Fragments arranged by size: 1133 976 240 BtgI C'CryG_G Cuts at: 0 386 510 576 605 1628 2153 2349 Size: 386 124 66 29 1023 525 196 Fragments arranged by size: 1023 525 386 196 124 66 29 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 84 727 2349 Size: 84 643 1622 Fragments arranged by size: 1622 643 84 BtsI GCAGTG_nn' Cuts at: 0 487 679 1284 1423 2100 2349 Size: 487 192 605 139 677 249 Fragments arranged by size: 677 605 487 249 192 139 DraIII CAC_nnn'GTG Cuts at: 0 1008 2349 Size: 1008 1341 EaeI y'GGCC_r Cuts at: 0 614 2009 2329 2349 Size: 614 1395 320 20 Fragments arranged by size: 1395 614 320 20 EarI CTCTTCn'nnn_ Cuts at: 0 2 1773 2349 Size: 2 1771 576 Fragments arranged by size: 1771 576 2 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 299 2349 Size: 299 2050 EcoICRI GAG'CTC Cuts at: 0 464 2349 Size: 464 1885 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 34 220 1000 1354 1456 1579 1620 1666 Size: 34 186 780 354 102 123 41 46 Cuts at: 1666 2214 2349 Size: 548 135 Fragments arranged by size: 780 548 354 186 135 123 102 46 41 34 EcoNI CCTnn'n_nnAGG Cuts at: 0 982 2349 Size: 982 1367 EcoO109I rG'GnC_Cy Cuts at: 0 338 727 2217 2218 2349 Size: 338 389 1490 1 131 Fragments arranged by size: 1490 389 338 131 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 324 2349 Size: 324 2025 HaeII r_GCGC'y Cuts at: 0 182 821 1433 2349 Size: 182 639 612 916 Fragments arranged by size: 916 639 612 182 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1828 1860 2349 Size: 1828 32 489 Fragments arranged by size: 1828 489 32 HindIII A'AGCT_T Cuts at: 0 1757 2349 Size: 1757 592 KasI G'GCGC_C Cuts at: 0 178 817 1429 2349 Size: 178 639 612 920 Fragments arranged by size: 920 639 612 178 KpnI G_GTAC'C Cuts at: 0 567 2349 Size: 567 1782 MscI TGG'CCA Cuts at: 0 616 2011 2349 Size: 616 1395 338 Fragments arranged by size: 1395 616 338 MslI CAynn'nnrTG Cuts at: 0 103 1231 1519 2071 2349 Size: 103 1128 288 552 278 Fragments arranged by size: 1128 552 288 278 103 MspA1I CmG'CkG Cuts at: 0 190 253 329 512 561 578 607 627 Size: 190 63 76 183 49 17 29 20 Cuts at: 627 827 1017 1485 1506 1557 1930 2090 2349 Size: 200 190 468 21 51 373 160 259 Fragments arranged by size: 468 373 259 200 190 190 183 160 76 63 51 49 29 21 20 17 NaeI GCC'GGC Cuts at: 0 991 2349 Size: 991 1358 NarI GG'CG_CC Cuts at: 0 179 818 1430 2349 Size: 179 639 612 919 Fragments arranged by size: 919 639 612 179 NcoI C'CATG_G Cuts at: 0 386 2349 Size: 386 1963 NgoMIV G'CCGG_C Cuts at: 0 989 2349 Size: 989 1360 NspI r_CATG'y Cuts at: 0 939 2349 Size: 939 1410 PasI CC'CwG_GG Cuts at: 0 813 2349 Size: 813 1536 PflMI CCAn_nnn'nTGG Cuts at: 0 1506 2349 Size: 1506 843 PfoI T'CCnGG_A Cuts at: 0 1136 1643 1676 2349 Size: 1136 507 33 673 Fragments arranged by size: 1136 673 507 33 PmlI CAC'GTG Cuts at: 0 473 905 1968 2238 2349 Size: 473 432 1063 270 111 Fragments arranged by size: 1063 473 432 270 111 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1342 1374 2349 Size: 1342 32 975 Fragments arranged by size: 1342 975 32 PpuMI rG'GwC_Cy Cuts at: 0 338 727 2349 Size: 338 389 1622 Fragments arranged by size: 1622 389 338 PspOMI G'GGCC_C Cuts at: 0 796 2217 2349 Size: 796 1421 132 Fragments arranged by size: 1421 796 132 PspXI vC'TCGA_Gb Cuts at: 0 544 2349 Size: 544 1805 PstI C_TGCA'G Cuts at: 0 173 603 1493 1556 1808 1897 2349 Size: 173 430 890 63 252 89 452 Fragments arranged by size: 890 452 430 252 173 89 63 PvuII CAG'CTG Cuts at: 0 253 627 1506 1557 1930 2090 2349 Size: 253 374 879 51 373 160 259 Fragments arranged by size: 879 374 373 259 253 160 51 SacI G_AGCT'C Cuts at: 0 466 2349 Size: 466 1883 SacII CC_GC'GG Cuts at: 0 513 579 608 2349 Size: 513 66 29 1741 Fragments arranged by size: 1741 513 66 29 SanDI GG'GwC_CC Cuts at: 0 727 2349 Size: 727 1622 SapI GCTCTTCn'nnn_ Cuts at: 0 2 2349 Size: 2 2347 ScaI AGT'ACT Cuts at: 0 1327 2349 Size: 1327 1022 SfcI C'TryA_G Cuts at: 0 169 599 930 1489 1552 1804 1893 2349 Size: 169 430 331 559 63 252 89 456 Fragments arranged by size: 559 456 430 331 252 169 89 63 SfoI GGC'GCC Cuts at: 0 180 819 1431 2349 Size: 180 639 612 918 Fragments arranged by size: 918 639 612 180 SmaI CCC'GGG Cuts at: 0 157 724 851 2349 Size: 157 567 127 1498 Fragments arranged by size: 1498 567 157 127 SmlI C'TyrA_G Cuts at: 0 355 544 706 2349 Size: 355 189 162 1643 Fragments arranged by size: 1643 355 189 162 StuI AGG'CCT Cuts at: 0 1060 2059 2349 Size: 1060 999 290 Fragments arranged by size: 1060 999 290 StyI C'CwwG_G Cuts at: 0 386 1229 1940 2349 Size: 386 843 711 409 Fragments arranged by size: 843 711 409 386 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1633 2003 2349 Size: 1633 370 346 Fragments arranged by size: 1633 370 346 TatI w'GTAC_w Cuts at: 0 311 938 1325 2349 Size: 311 627 387 1024 Fragments arranged by size: 1024 627 387 311 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1690 1722 2092 2115 2124 2147 2294 2326 Size: 1690 32 370 23 9 23 147 32 Cuts at: 2326 2349 Size: 23 Fragments arranged by size: 1690 370 147 32 32 23 23 23 9 Tth111I GACn'n_nGTC Cuts at: 0 2245 2349 Size: 2245 104 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 744 1317 1503 2019 2349 Size: 744 573 186 516 330 Fragments arranged by size: 744 573 516 330 186 XhoI C'TCGA_G Cuts at: 0 544 2349 Size: 544 1805 XmaI C'CCGG_G Cuts at: 0 155 722 849 2349 Size: 155 567 127 1500 Fragments arranged by size: 1500 567 155 127 ZraI GAC'GTC Cuts at: 0 596 681 2349 Size: 596 85 1668 Fragments arranged by size: 1668 596 85 Enzymes that do cut: AarI AatII Acc65I AcuI AflIII AgeI AleI AlfI AloI AlwNI ApaI ApaLI AvaI BanI BanII BbeI BbsI BbvCI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI BtgI BtgZI BtsI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HindIII KasI KpnI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PflMI PfoI PmlI PpiI PpuMI PspOMI PspXI PstI PvuII SacI SacII SanDI SapI ScaI SfcI SfoI SmaI SmlI StuI StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI ZraI Enzymes that do not cut: AccI AclI AfeI AflII AhdI ApoI AscI AseI AsiSI AvrII BaeI BamHI BcgI BciVI BfrBI BmgBI BmtI BsaBI BsiWI BsmI BsmBI BspEI BspHI BsrDI BssHII BstBI BstZ17I Bsu36I ClaI CspCI DraI DrdI EagI EcoRI EcoRV FseI FspI FspAI HincII HpaI MfeI MluI MmeI NdeI NheI NotI NruI NsiI PacI PciI PmeI PshAI PsiI PsrI PvuI RsrII SalI SbfI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI XbaI XmnI