(Circular) (Six-base) MAPSORT of: pFN21AE3100.seq Check: 9361 from: 1 to: 10810 pFN21AE3100 10810 bp With 182 enzymes: SgfI * March 28, 2012 14:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3316 4444 6355 3316 Size: 1128 1911 7771 Fragments arranged by size: 7771 1911 1128 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2847 8620 278 Size: 53 83 186 936 1311 5773 2468 Fragments arranged by size: 5773 2468 1311 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2625 3887 6750 8153 1937 Size: 688 1262 2863 1403 4594 Fragments arranged by size: 4594 2863 1403 1262 688 Acc65I G'GTAC_C Cuts at: 2508 3075 3722 8131 2508 Size: 567 647 4409 5187 Fragments arranged by size: 5187 4409 647 567 AclI AA'CG_TT Cuts at: 1563 8937 9310 1563 Size: 7374 373 3063 Fragments arranged by size: 7374 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3854 4071 4190 4404 7577 7618 8873 10257 Size: 2222 217 119 214 3173 41 1255 1384 Cuts at: 10257 10525 1632 Size: 268 1917 Fragments arranged by size: 3173 2222 1917 1384 1255 268 217 214 119 41 AflII C'TTAA_G Cuts at: 820 1017 4508 820 Size: 197 3491 7122 Fragments arranged by size: 7122 3491 197 AflIII A'CryG_T Cuts at: 1203 2184 2808 3882 9709 10686 1203 Size: 981 624 1074 5827 977 1327 Fragments arranged by size: 5827 1327 1074 981 977 624 AgeI A'CCGG_T Cuts at: 3473 6478 9641 3473 Size: 3005 3163 4642 Fragments arranged by size: 4642 3163 3005 AhdI GACnn_n'nnGTC Cuts at: 4368 9539 4368 Size: 5171 5639 AleI CACnn'nnGTG Cuts at: 1166 3731 3962 3992 4358 4409 5036 5138 5773 Size: 2565 231 30 366 51 627 102 635 Cuts at: 5773 5810 5839 6713 7286 7991 1166 Size: 37 29 874 573 705 3985 Fragments arranged by size: 3985 2565 874 705 635 627 573 366 231 102 51 37 30 29 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 5791 5857 6075 6239 1184 Size: 4607 66 218 164 5755 Fragments arranged by size: 5755 4607 218 164 66 AlwNI CAG_nnn'CTG Cuts at: 2549 3322 3779 3923 4427 5816 6544 6562 6901 Size: 773 457 144 504 1389 728 18 339 Cuts at: 6901 7075 7102 7570 8553 10125 2549 Size: 174 27 468 983 1572 3234 Fragments arranged by size: 3234 1572 1389 983 773 728 504 468 457 339 174 144 27 18 ApaI G_GGCC'C Cuts at: 2400 4149 4519 5560 6491 7632 7684 7869 2400 Size: 1749 370 1041 931 1141 52 185 5341 Fragments arranged by size: 5341 1749 1141 1041 931 370 185 52 ApaLI G'TGCA_C Cuts at: 3908 8867 10023 3908 Size: 4959 1156 4695 Fragments arranged by size: 4959 4695 1156 ApoI r'AATT_y Cuts at: 1493 8119 8345 8497 10606 1493 Size: 6626 226 152 2109 1697 Fragments arranged by size: 6626 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 9364 160 Size: 872 8332 1606 Fragments arranged by size: 8332 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10810 AvaI C'yCGr_G Cuts at: 1870 1943 2417 2601 2967 3108 4519 5606 6038 Size: 73 474 184 366 141 1411 1087 432 Cuts at: 6038 6484 6830 8135 1870 Size: 446 346 1305 4545 Fragments arranged by size: 4545 1411 1305 1087 474 446 432 366 346 184 141 73 AvrII C'CTAG_G Cuts at: 4651 4651 Size: 10810 BamHI G'GATC_C Cuts at: 8140 8507 8140 Size: 367 10443 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2089 2508 2755 2824 3075 Size: 325 217 627 302 419 247 69 251 Cuts at: 3075 3403 3694 3722 4349 4532 4631 4667 4745 Size: 328 291 28 627 183 99 36 78 Cuts at: 4745 4897 5075 5261 5348 5387 5429 5697 5767 Size: 152 178 186 87 39 42 268 70 Cuts at: 5767 5903 5987 6101 6125 6190 6215 6287 6797 Size: 136 84 114 24 65 25 72 510 Cuts at: 6797 6947 7008 7463 7532 8030 8131 9586 618 Size: 150 61 455 69 498 101 1455 1842 Fragments arranged by size: 1842 1455 627 627 510 498 455 419 328 325 302 291 268 251 247 217 186 183 178 152 150 136 114 101 99 87 84 78 72 70 69 69 65 61 42 39 36 28 25 24 BanII G_rGCy'C Cuts at: 1391 2136 2400 3287 4004 4149 4519 4836 5489 Size: 745 264 887 717 145 370 317 653 Cuts at: 5489 5560 5607 5760 6491 6633 6825 6960 7632 Size: 71 47 153 731 142 192 135 672 Cuts at: 7632 7684 7799 7818 7826 7869 8129 1391 Size: 52 115 19 8 43 260 4072 Fragments arranged by size: 4072 887 745 731 717 672 653 370 317 264 260 192 153 145 142 135 115 71 52 47 43 19 8 BbeI G_GCGC'C Cuts at: 2759 4353 5391 5701 2759 Size: 1594 1038 310 7868 Fragments arranged by size: 7868 1594 1038 310 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3743 3842 928 Size: 971 1844 99 7896 Fragments arranged by size: 7896 1844 971 99 BbvCI CC'TCA_GC Cuts at: 4183 4183 Size: 10810 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7747 7781 9001 9035 1903 Size: 34 5810 34 1220 34 3678 Fragments arranged by size: 5810 3678 1220 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2902 2932 8702 9912 2902 Size: 30 5770 1210 3800 Fragments arranged by size: 5770 3800 1210 30 BclI T'GATC_A Cuts at: 1548 4773 4890 1548 Size: 3225 117 7468 Fragments arranged by size: 7468 3225 117 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3527 3650 3659 Size: 107 122 71 631 605 1855 123 9 Cuts at: 3659 4232 5414 5465 5555 5831 6553 6911 7841 Size: 573 1182 51 90 276 722 358 930 Cuts at: 7841 9421 136 Size: 1580 1525 Fragments arranged by size: 1855 1580 1525 1182 930 722 631 605 573 358 276 123 122 107 90 71 51 9 BglII A'GATC_T Cuts at: 10805 10805 Size: 10810 BlpI GC'TnA_GC Cuts at: 5663 8227 5663 Size: 2564 8246 Bme1580I G_kGCm'C Cuts at: 1790 2400 2424 3429 3601 3912 4149 4519 4554 Size: 610 24 1005 172 311 237 370 35 Cuts at: 4554 4748 5264 5351 5560 5846 5990 6029 6130 Size: 194 516 87 209 286 144 39 101 Cuts at: 6130 6290 6491 6950 7011 7466 7535 7632 7684 Size: 160 201 459 61 455 69 97 52 Cuts at: 7684 7869 8871 10027 1790 Size: 185 1002 1156 2573 Fragments arranged by size: 2573 1156 1005 1002 610 516 459 455 370 311 286 237 209 201 194 185 172 160 144 101 97 87 69 61 52 39 35 24 BmgBI CAC'GTC Cuts at: 1318 2727 3885 7659 1318 Size: 1409 1158 3774 4469 Fragments arranged by size: 4469 3774 1409 1158 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2733 4272 5472 Size: 416 53 83 399 24 1316 1539 1200 Cuts at: 5472 5491 8541 9499 442 Size: 19 3050 958 1753 Fragments arranged by size: 3050 1753 1539 1316 1200 958 416 399 83 53 24 19 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 10810 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7287 7759 7287 Size: 472 10338 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2327 2571 2966 3164 3173 3179 4449 5492 Size: 1088 244 395 198 9 6 1270 1043 Cuts at: 5492 5771 5849 6394 6500 6905 7879 8049 9470 Size: 279 78 545 106 405 974 170 1421 Cuts at: 9470 1239 Size: 2579 Fragments arranged by size: 2579 1421 1270 1088 1043 974 545 405 395 279 244 198 170 106 78 9 6 Bpu10I CC'TnA_GC Cuts at: 4183 6627 4183 Size: 2444 8366 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3429 4747 7802 8935 9800 10098 3429 Size: 1318 3055 1133 865 298 4141 Fragments arranged by size: 4141 3055 1318 1133 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2918 5054 5240 5327 5882 5966 6266 Size: 617 1419 2136 186 87 555 84 300 Cuts at: 6266 9473 882 Size: 3207 2219 Fragments arranged by size: 3207 2219 2136 1419 617 555 300 186 87 84 BsaAI yAC'GTr Cuts at: 493 1204 2185 2809 8601 493 Size: 711 981 624 5792 2702 Fragments arranged by size: 5792 2702 981 711 624 BsaBI GATnn'nnATC Cuts at: 5447 10804 5447 Size: 5357 5453 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2384 2756 2844 4106 Size: 53 83 186 936 851 372 88 1262 Cuts at: 4106 4350 5388 5698 6141 8617 8999 9634 275 Size: 244 1038 310 443 2476 382 635 1451 Fragments arranged by size: 2476 1451 1262 1038 936 851 635 443 382 372 310 244 186 88 83 53 BsaWI w'CCGG_w Cuts at: 2086 3250 3473 5445 6478 7711 9243 9641 9915 Size: 1164 223 1972 1033 1233 1532 398 274 Cuts at: 9915 10062 10473 2086 Size: 147 411 2423 Fragments arranged by size: 2423 1972 1532 1233 1164 1033 411 398 274 223 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2414 2444 2861 2891 3007 Size: 30 165 30 1011 30 417 30 116 Cuts at: 3007 3037 3122 3152 3481 3511 1178 Size: 30 85 30 329 30 8477 Fragments arranged by size: 8477 1011 417 329 165 116 85 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3784 6848 1186 Size: 2598 3064 5148 Fragments arranged by size: 5148 3064 2598 BseYI C'CCAG_C Cuts at: 2218 2689 2820 3103 3153 3292 3530 3715 3772 Size: 471 131 283 50 139 238 185 57 Cuts at: 3772 3936 4869 4917 5127 5750 5801 6194 7003 Size: 164 933 48 210 623 51 393 809 Cuts at: 7003 7119 7180 7226 7296 7318 7406 7805 7836 Size: 116 61 46 70 22 88 399 31 Cuts at: 7836 7868 10013 10509 2218 Size: 32 2145 496 2519 Fragments arranged by size: 2519 2145 933 809 623 496 471 399 393 283 238 210 185 164 139 131 116 88 70 61 57 51 50 48 46 32 31 22 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2165 2271 3335 5036 5162 5442 5651 Size: 573 429 106 1064 1701 126 280 209 Cuts at: 5651 7109 7907 1163 Size: 1458 798 4066 Fragments arranged by size: 4066 1701 1458 1064 798 573 429 280 209 126 106 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2016 3663 4803 5571 6639 7020 Size: 275 186 15 1647 1140 768 1068 381 Cuts at: 7020 8260 9021 10049 1540 Size: 1240 761 1028 2301 Fragments arranged by size: 2301 1647 1240 1140 1068 1028 768 761 381 275 186 15 BsiHKAI G_wGCw'C Cuts at: 2136 2968 3912 4030 4435 5045 5567 5957 6257 Size: 832 944 118 405 610 522 390 300 Cuts at: 6257 6380 6633 6921 7723 7799 7818 7826 8871 Size: 123 253 288 802 76 19 8 1045 Cuts at: 8871 8956 10027 2136 Size: 85 1071 2919 Fragments arranged by size: 2919 1071 1045 944 832 802 610 522 405 390 300 288 253 123 118 85 76 19 8 BsmI GAATG_Cn' Cuts at: 5935 8321 8414 5935 Size: 2386 93 8331 Fragments arranged by size: 8331 2386 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4276 4594 4678 5249 5336 5975 6275 4276 Size: 318 84 571 87 639 300 8811 Fragments arranged by size: 8811 639 571 318 300 87 84 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2136 2400 2424 2968 3287 3429 3601 Size: 399 346 264 24 544 319 142 172 Cuts at: 3601 3912 4004 4030 4149 4435 4519 4554 4748 Size: 311 92 26 119 286 84 35 194 Cuts at: 4748 4836 5045 5264 5351 5489 5560 5567 5607 Size: 88 209 219 87 138 71 7 40 Cuts at: 5607 5760 5846 5957 5990 6029 6130 6257 6290 Size: 153 86 111 33 39 101 127 33 Cuts at: 6290 6380 6491 6633 6825 6921 6950 6960 7011 Size: 90 111 142 192 96 29 10 51 Cuts at: 7011 7466 7535 7632 7684 7723 7799 7818 7826 Size: 455 69 97 52 39 76 19 8 Cuts at: 7826 7869 8129 8871 8956 10027 1391 Size: 43 260 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 544 455 399 346 319 311 286 264 260 219 209 194 192 172 153 142 142 138 127 119 111 111 101 97 96 92 90 88 87 86 85 84 76 71 69 52 51 43 40 39 39 35 33 33 29 26 24 19 10 8 7 BspEI T'CCGG_A Cuts at: 5445 7711 10473 5445 Size: 2266 2762 5782 Fragments arranged by size: 5782 2762 2266 BspHI T'CATG_A Cuts at: 8699 10429 8699 Size: 1730 9080 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3316 3526 4444 6355 7401 8165 844 Size: 2472 210 918 1911 1046 764 3489 Fragments arranged by size: 3489 2472 1911 1046 918 764 210 BsrBI CCG'CTC Cuts at: 1394 1823 8697 1394 Size: 429 6874 3507 Fragments arranged by size: 6874 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2332 4749 7969 9305 9479 65 Size: 1183 1084 2417 3220 1336 174 1396 Fragments arranged by size: 3220 2417 1396 1336 1183 1084 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2352 3473 5570 6478 6689 6950 7214 Size: 110 542 1121 2097 908 211 261 264 Cuts at: 7214 7300 9454 9641 1700 Size: 86 2154 187 2869 Fragments arranged by size: 2869 2154 2097 1121 908 542 264 261 211 187 110 86 BsrGI T'GTAC_A Cuts at: 96 2187 3006 96 Size: 2091 819 7900 Fragments arranged by size: 7900 2091 819 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10810 BssSI C'ACGA_G Cuts at: 2861 5066 5252 5339 5894 5978 6278 8870 9882 Size: 2205 186 87 555 84 300 2592 1012 Cuts at: 9882 2861 Size: 3789 Fragments arranged by size: 3789 2592 2205 1012 555 300 186 87 84 BstAPI GCAn_nnn'nTGC Cuts at: 794 2448 6562 7102 7570 794 Size: 1654 4114 540 468 4034 Fragments arranged by size: 4114 4034 1654 540 468 BstBI TT'CG_AA Cuts at: 5231 9614 5231 Size: 4383 6427 BstEII G'GTnAC_C Cuts at: 1184 3505 3952 3973 4177 1184 Size: 2321 447 21 204 7817 Fragments arranged by size: 7817 2321 447 204 21 BstXI CCAn_nnnn'nTGG Cuts at: 2890 2935 3142 3964 3991 4255 5072 5207 6715 Size: 45 207 822 27 264 817 135 1508 Cuts at: 6715 6811 7234 7474 2890 Size: 96 423 240 6226 Fragments arranged by size: 6226 1508 822 817 423 264 240 207 135 96 45 27 BstYI r'GATC_y Cuts at: 1972 3678 6646 8140 8507 8892 8909 10361 10447 Size: 1706 2968 1494 367 385 17 1452 86 Cuts at: 10447 10459 10805 1972 Size: 12 346 1977 Fragments arranged by size: 2968 1977 1706 1494 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3480 3480 Size: 10810 BtgI C'CryG_G Cuts at: 513 1065 2004 2093 2104 2118 2619 2913 3216 Size: 552 939 89 11 14 501 294 303 Cuts at: 3216 3699 3741 4110 4353 4477 4554 6162 6551 Size: 483 42 369 243 124 77 1608 389 Cuts at: 6551 6642 6882 6960 513 Size: 91 240 78 4363 Fragments arranged by size: 4363 1608 939 552 501 483 389 369 303 294 243 240 124 91 89 78 77 42 14 11 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4975 7936 487 Size: 683 56 3749 2961 3361 Fragments arranged by size: 3749 3361 2961 683 56 BtsI GCAGTG_nn' Cuts at: 2977 3168 4030 4449 4782 5358 5834 6849 8326 Size: 191 862 419 333 576 476 1015 1477 Cuts at: 8326 9120 9140 2977 Size: 794 20 4647 Fragments arranged by size: 4647 1477 1015 862 794 576 476 419 333 191 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 10810 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4603 4638 4666 4701 5083 5118 7600 Size: 35 4039 35 28 35 382 35 2482 Cuts at: 7600 7635 7979 8014 529 Size: 35 344 35 3325 Fragments arranged by size: 4039 3325 2482 382 344 35 35 35 35 35 35 28 DraI TTT'AAA Cuts at: 8114 8114 Size: 10810 DraIII CAC_nnn'GTG Cuts at: 3596 4904 5057 5885 3596 Size: 1308 153 828 8521 Fragments arranged by size: 8521 1308 828 153 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6166 6430 9817 809 Size: 507 4850 264 3387 1802 Fragments arranged by size: 4850 3387 1802 507 264 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2013 2107 2121 Size: 54 1425 50 275 18 183 94 14 Cuts at: 2121 2469 2540 2853 2974 3642 3651 3660 4329 Size: 348 71 313 121 668 9 9 669 Cuts at: 4329 4474 4722 4800 5088 5298 5457 5547 5568 Size: 145 248 78 288 210 159 90 21 Cuts at: 5568 5850 6000 6300 6636 6705 6780 6903 7017 Size: 282 150 300 336 69 75 123 114 Cuts at: 7017 7212 7254 7311 7356 7621 7785 7983 8257 Size: 195 42 57 45 265 164 198 274 Cuts at: 8257 9146 10775 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 669 668 348 336 313 300 288 282 275 274 265 248 210 198 195 183 164 159 150 145 123 121 114 94 90 78 75 71 69 57 54 50 45 43 42 21 18 14 9 9 EagI C'GGCC_G Cuts at: 1537 1812 2013 3660 4800 5568 6636 7017 8257 Size: 275 201 1647 1140 768 1068 381 1240 Cuts at: 8257 1537 Size: 4090 Fragments arranged by size: 4090 1647 1240 1140 1068 768 381 275 201 EarI CTCTTCn'nnn_ Cuts at: 1353 2360 2766 3992 8740 1353 Size: 1007 406 1226 4748 3423 Fragments arranged by size: 4748 3423 1226 1007 406 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3862 4582 6586 7013 9397 9771 Size: 1064 138 2273 720 2004 427 2384 374 Cuts at: 9771 9917 387 Size: 146 1280 Fragments arranged by size: 2384 2273 2004 1280 1064 720 427 374 146 138 EcoICRI GAG'CTC Cuts at: 2134 6631 7797 7816 7824 2134 Size: 4497 1166 19 8 5120 Fragments arranged by size: 5120 4497 1166 19 8 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2327 2571 2966 3164 3173 3179 3854 Size: 393 695 244 395 198 9 6 675 Cuts at: 3854 4071 4190 4404 4449 5492 5771 5849 6394 Size: 217 119 214 45 1043 279 78 545 Cuts at: 6394 6500 6905 7577 7618 7879 8049 8873 9470 Size: 106 405 672 41 261 170 824 597 Cuts at: 9470 10257 10525 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1043 824 787 695 675 672 597 545 405 395 393 279 268 261 244 217 214 198 170 119 106 78 45 41 9 6 EcoNI CCTnn'n_nnAGG Cuts at: 3350 3350 Size: 10810 EcoO109I rG'GnC_Cy Cuts at: 2397 2485 2587 2695 3067 4115 4145 4146 4515 Size: 88 102 108 372 1048 30 1 369 Cuts at: 4515 4516 5556 5557 5683 6488 6530 6604 6898 Size: 1 1040 1 126 805 42 74 294 Cuts at: 6898 7150 7628 7629 7680 7681 2397 Size: 252 478 1 51 1 5526 Fragments arranged by size: 5526 1048 1040 805 478 372 369 294 252 126 108 102 88 74 51 42 30 1 1 1 1 1 EcoRI G'AATT_C Cuts at: 8119 8497 8119 Size: 378 10432 FseI GG_CCGG'CC Cuts at: 5574 5574 Size: 10810 FspI TGC'GCA Cuts at: 2983 9316 2983 Size: 6333 4477 HaeII r_GCGC'y Cuts at: 1678 2759 4353 5391 5471 5701 5943 7241 9957 Size: 1081 1594 1038 80 230 242 1298 2716 Cuts at: 9957 1678 Size: 2531 Fragments arranged by size: 2716 2531 1594 1298 1081 1038 242 230 80 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3463 3495 4024 4056 4354 Size: 32 135 32 1891 32 529 32 298 Cuts at: 4354 4386 6593 6625 6743 6775 9451 9483 9525 Size: 32 2207 32 118 32 2676 32 42 Cuts at: 9525 9557 1373 Size: 32 2626 Fragments arranged by size: 2676 2626 2207 1891 529 298 135 118 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 8154 8400 669 Size: 988 281 6216 246 3079 Fragments arranged by size: 6216 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 10810 HpaI GTT'AAC Cuts at: 8400 8400 Size: 10810 KasI G'GCGC_C Cuts at: 2755 4349 5387 5697 2755 Size: 1594 1038 310 7868 Fragments arranged by size: 7868 1594 1038 310 KpnI G_GTAC'C Cuts at: 2512 3079 3726 8135 2512 Size: 567 647 4409 5187 Fragments arranged by size: 5187 4409 647 567 MfeI C'AATT_G Cuts at: 7195 8409 7195 Size: 1214 9596 MluI A'CGCG_T Cuts at: 10686 10686 Size: 10810 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5070 5316 5898 5982 6282 6342 7302 7929 Size: 3765 246 582 84 300 60 960 627 Cuts at: 7929 9924 10108 1305 Size: 1995 184 2007 Fragments arranged by size: 3765 2007 1995 960 627 582 300 246 184 84 60 MscI TGG'CCA Cuts at: 10 64 1489 1832 2123 2471 2855 2976 4476 Size: 54 1425 343 291 348 384 121 1500 Cuts at: 4476 4724 5459 5549 6707 6905 7313 7358 7787 Size: 248 735 90 1158 198 408 45 429 Cuts at: 7787 10 Size: 3033 Fragments arranged by size: 3033 1500 1425 1158 735 429 408 384 348 343 291 248 198 121 90 54 45 MslI CAynn'nnrTG Cuts at: 518 1166 2141 2933 3422 3731 3746 3827 3905 Size: 648 975 792 489 309 15 81 78 Cuts at: 3905 3962 3992 4322 4358 4409 4793 4853 5036 Size: 57 30 330 36 51 384 60 183 Cuts at: 5036 5138 5534 5540 5706 5773 5810 5839 6107 Size: 102 396 6 166 67 37 29 268 Cuts at: 6107 6713 6809 7286 7853 7991 8768 9127 9286 Size: 606 96 477 567 138 777 359 159 Cuts at: 9286 518 Size: 2042 Fragments arranged by size: 2042 975 792 777 648 606 567 489 477 396 384 359 330 309 268 183 166 159 138 102 96 81 78 67 60 57 51 37 36 30 29 15 6 MspA1I CmG'CkG Cuts at: 1606 2039 2058 2748 3322 3656 3665 3719 4157 Size: 433 19 690 574 334 9 54 438 Cuts at: 4157 4823 5171 5426 5723 6544 6866 7102 7555 Size: 666 348 255 297 821 322 236 453 Cuts at: 7555 7840 8226 8903 10051 10296 1606 Size: 285 386 677 1148 245 2120 Fragments arranged by size: 2120 1148 821 690 677 666 574 453 438 433 386 348 334 322 297 285 255 245 236 54 19 9 NaeI GCC'GGC Cuts at: 5572 6691 7216 5572 Size: 1119 525 9166 Fragments arranged by size: 9166 1119 525 NarI GG'CG_CC Cuts at: 2756 4350 5388 5698 2756 Size: 1594 1038 310 7868 Fragments arranged by size: 7868 1594 1038 310 NcoI C'CATG_G Cuts at: 513 1065 2004 2913 3741 4554 6960 513 Size: 552 939 909 828 813 2406 4363 Fragments arranged by size: 4363 2406 939 909 828 813 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 10810 NgoMIV G'CCGG_C Cuts at: 5570 6689 7214 5570 Size: 1119 525 9166 Fragments arranged by size: 9166 1119 525 NheI G'CTAG_C Cuts at: 1052 1052 Size: 10810 NotI GC'GGCC_GC Cuts at: 3660 8257 3660 Size: 4597 6213 NspI r_CATG'y Cuts at: 1132 1229 3832 8168 9713 1132 Size: 97 2603 4336 1545 2229 Fragments arranged by size: 4336 2603 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2243 3601 5482 6382 7375 7676 1348 Size: 895 1358 1881 900 993 301 4482 Fragments arranged by size: 4482 1881 1358 993 900 895 301 PciI A'CATG_T Cuts at: 9709 9709 Size: 10810 PflMI CCAn_nnn'nTGG Cuts at: 1108 2208 2549 2919 5582 6901 1108 Size: 1100 341 370 2663 1319 5017 Fragments arranged by size: 5017 2663 1319 1100 370 341 PfoI T'CCnGG_A Cuts at: 2529 2529 Size: 10810 PmeI GTTT'AAAC Cuts at: 8114 8114 Size: 10810 PmlI CAC'GTG Cuts at: 2185 2185 Size: 10810 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 8879 8911 10392 10424 8879 Size: 32 1481 32 9265 Fragments arranged by size: 9265 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2485 2587 2695 3067 4115 5683 6530 6604 2485 Size: 102 108 372 1048 1568 847 74 6691 Fragments arranged by size: 6691 1568 1048 847 372 108 102 74 PshAI GACnn'nnGTC Cuts at: 1613 5864 1613 Size: 4251 6559 PsiI TTA'TAA Cuts at: 8380 8380 Size: 10810 PspOMI G'GGCC_C Cuts at: 2396 4145 4515 5556 6487 7628 7680 7865 2396 Size: 1749 370 1041 931 1141 52 185 5341 Fragments arranged by size: 5341 1749 1141 1041 931 370 185 52 PstI C_TGCA'G Cuts at: 830 2052 2687 3844 6567 6859 7107 7332 7339 Size: 1222 635 1157 2723 292 248 225 7 Cuts at: 7339 7575 8007 8162 830 Size: 236 432 155 3478 Fragments arranged by size: 3478 2723 1222 1157 635 432 292 248 236 225 155 7 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10810 PvuII CAG'CTG Cuts at: 5171 5426 6544 6866 7102 7555 7840 5171 Size: 255 1118 322 236 453 285 8141 Fragments arranged by size: 8141 1118 453 322 285 255 236 SacI G_AGCT'C Cuts at: 2136 6633 7799 7818 7826 2136 Size: 4497 1166 19 8 5120 Fragments arranged by size: 5120 4497 1166 19 8 SalI G'TCGA_C Cuts at: 1936 8152 1936 Size: 6216 4594 SapI GCTCTTCn'nnn_ Cuts at: 3992 3992 Size: 10810 SbfI CC_TGCA'GG Cuts at: 8162 8162 Size: 10810 ScaI AGT'ACT Cuts at: 7772 9058 10770 7772 Size: 1286 1712 7812 Fragments arranged by size: 7812 1712 1286 SexAI A'CCwGG_T Cuts at: 3051 3051 Size: 10810 SfcI C'TryA_G Cuts at: 826 1045 2048 2683 3840 4962 5367 6563 6855 Size: 219 1003 635 1157 1122 405 1196 292 Cuts at: 6855 7103 7328 7335 7571 8003 8158 9293 9974 Size: 248 225 7 236 432 155 1135 681 Cuts at: 9974 10165 826 Size: 191 1471 Fragments arranged by size: 1471 1196 1157 1135 1122 1003 681 635 432 405 292 248 236 225 219 191 155 7 SfiI GGCCn_nnn'nGGCC Cuts at: 3650 3659 5555 7841 3650 Size: 9 1896 2286 6619 Fragments arranged by size: 6619 2286 1896 9 SfoI GGC'GCC Cuts at: 2757 4351 5389 5699 2757 Size: 1594 1038 310 7868 Fragments arranged by size: 7868 1594 1038 310 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10810 SgrAI Cr'CCGG_yG Cuts at: 1700 6950 1700 Size: 5250 5560 SmaI CCC'GGG Cuts at: 1872 2603 4521 5608 6486 8137 1872 Size: 731 1918 1087 878 1651 4545 Fragments arranged by size: 4545 1918 1651 1087 878 731 SmlI C'TyrA_G Cuts at: 820 1017 1943 3444 4508 4762 7817 8914 9815 Size: 197 926 1501 1064 254 3055 1097 901 Cuts at: 9815 10077 820 Size: 262 1553 Fragments arranged by size: 3055 1553 1501 1097 1064 926 901 262 254 197 SnaBI TAC'GTA Cuts at: 493 8601 493 Size: 8108 2702 SpeI A'CTAG_T Cuts at: 152 4709 152 Size: 4557 6253 SphI G_CATG'C Cuts at: 1132 3832 8168 1132 Size: 2700 4336 3774 Fragments arranged by size: 4336 3774 2700 SspI AAT'ATT Cuts at: 5 52 8734 5 Size: 47 8682 2081 Fragments arranged by size: 8682 2081 47 StuI AGG'CCT Cuts at: 1517 3041 1517 Size: 1524 9286 StyI C'CwwG_G Cuts at: 513 1065 2004 2504 2913 3741 4407 4554 4651 Size: 552 939 500 409 828 666 147 97 Cuts at: 4651 4740 5034 5113 5581 6960 8249 513 Size: 89 294 79 468 1379 1289 3074 Fragments arranged by size: 3074 1379 1289 939 828 666 552 500 468 409 294 147 97 89 79 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2235 2812 3259 3686 3712 4355 4892 Size: 1255 655 577 447 427 26 643 537 Cuts at: 4892 5150 5735 6008 6103 6113 6208 6395 6748 Size: 258 585 273 95 10 95 187 353 Cuts at: 6748 7364 7367 7420 8837 8854 9007 9192 9637 Size: 616 3 53 1417 17 153 185 445 Cuts at: 9637 325 Size: 1498 Fragments arranged by size: 1498 1417 1255 655 643 616 585 577 537 447 445 427 353 273 258 187 185 153 95 95 53 26 17 10 3 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2187 3006 3112 Size: 274 80 33 51 1443 210 819 106 Cuts at: 3112 7770 7857 9056 10768 96 Size: 4658 87 1199 1712 138 Fragments arranged by size: 4658 1712 1443 1199 819 274 210 138 106 87 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2414 2446 2861 2893 3007 3039 3433 3465 7421 Size: 32 415 32 114 32 394 32 3956 Cuts at: 7421 7453 7473 7505 10770 10802 2414 Size: 32 20 32 3265 32 2422 Fragments arranged by size: 3956 3265 2422 415 394 114 32 32 32 32 32 32 32 20 Tth111I GACn'n_nGTC Cuts at: 1316 6469 1316 Size: 5153 5657 XbaI T'CTAG_A Cuts at: 8146 8146 Size: 10810 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3784 4738 5101 5293 5467 5542 6041 6583 6808 Size: 954 363 192 174 75 499 542 225 Cuts at: 6808 7150 7264 7285 3784 Size: 342 114 21 7309 Fragments arranged by size: 7309 954 542 499 363 342 225 192 174 114 75 21 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 10810 XmaI C'CCGG_G Cuts at: 1870 2601 4519 5606 6484 8135 1870 Size: 731 1918 1087 878 1651 4545 Fragments arranged by size: 4545 1918 1651 1087 878 731 XmnI GAAnn'nnTTC Cuts at: 8939 8939 Size: 10810 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2845 8618 276 Size: 53 83 186 936 1311 5773 2468 Fragments arranged by size: 5773 2468 1311 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BfrBI BsiWI BstZ17I EcoRV FalI FspAI NruI NsiI PacI PspXI PsrI RsrII SacII SanDI SrfI SwaI