Restriction map for
pFN21AE9435
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# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AvrII BamHI
BbeI BbrPI BciVI BclI BcnI
BfrI BglI BglII BlnI BsaHI
BsaI BsgI BsiWI BsmBI BsmFI
BsmI BspDI BsrBI BssHII Bst1107I
BstBI BstZ17I Bsu36I ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoRV
EcoT22I FbaI FseI HaeII HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MfeI MluI
MseI MunI NaeI NarI NciI
NdeI NgoMIV NheI NotI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SspI
StuI SwaI Tru9I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AflIII 488 ACATGT A^C[GA][CT]GT
AvaI 1633 CCCGAG C^[CT]CG[GA]G
AviII 425 TGCGCA TGC^GCA
BbsI 559 GTCTTC GAAGAC(2/6)
BbvCI 691 GCTGAGG CCTCAGC(-5/-2)
BlpI 1537 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1537 GCTGAGC GC^T[ACGT]AGC
BpuAI 559 GTCTTC GAAGAC(2/6)
BsaAI 622 TACGTG [CT]AC^GT[GA]
BsaBI 102 GATGGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 1137 CGACCG CG[GA][CT]^CG
BsoBI 1633 CCCGAG C^[CT]CG[GA]G
Bsp1407I 491 TGTACA T^GTACA
BspHI 461 TCATGA T^CATGA
BspLU11I 488 ACATGT A^CATGT
BsrFI 72 ACCGGC [GA]^CCGG[CT]
BsrGI 491 TGTACA T^GTACA
BssSI 1037 CTCGTG CACGAG(-5/-1)
BstAPI 428 GCAATACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 582 CATCC GGATG(2/0)
CelII 1537 GCTGAGC GC^T[ACGT]AGC
Cfr10I 72 ACCGGC [GA]^CCGG[CT]
DraII 3 GGGGCCC [GA]G^G[ACGT]CC[CT]
DraIII 1654 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 140 CCATGG C^C[GA][CT]GG
EarI 905 GAAGAG CTCTTC(1/4)
EcoO109I 3 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoRI 180 GAATTC G^AATTC
FauI 1007 CCCGC CCCGC(4/6)
FokI 582 CATCC GGATG(9/13)
FspI 425 TGCGCA TGC^GCA
Ksp632I 905 GAAGAG CTCTTC(1/4)
MamI 102 GATGGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 140 CCATGG C^CATGG
NruI 1026 TCGCGA TCG^CGA
PflMI 1105 CCATTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 461 TCATGA T^CATGA
SacI 1612 GAGCTC GAGCT^C
SbfI 661 CCTGCAGG CCTGCA^GG
SmlI 1252 CTCAAG C^T[CT][GA]AG
SphI 258 GCATGC GCATG^C
Sse8387I 661 CCTGCAGG CCTGCA^GG
SspBI 491 TGTACA T^GTACA
TaqI 506 TCGA T^CGA
TfiI 672 GAATC G^A[AT]TC
TthHB8I 506 TCGA T^CGA
Van91I 1105 CCATTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 121 GTAGAC GT^[AC][GT]AC
1309 GTCTAC
AccIII 176 TCCGGA T^CCGGA
1094 TCCGGA
AcsI 180 GAATTC [GA]^AATT[CT]
897 GAATTT
ApaI 4 GGGCCC GGGCC^C
1708 GGGCCC
ApoI 180 GAATTC [GA]^AATT[CT]
897 GAATTT
AspHI 763 GTGCTC G[AT]GC[AT]^C
1612 GAGCTC
AspI 1345 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1366 GACTCTGTC
AvaII 635 GGACC G^G[AT]CC
917 GGACC
BfaI 13 CTAG C^TAG
1694 CTAG
BpmI 400 CTCCAG CTGGAG(16/14)
1735 CTGGAG
BsaWI 176 TCCGGA [AT]^CCGG[AT]
1094 TCCGGA
BseAI 176 TCCGGA T^CCGGA
1094 TCCGGA
BsiHKAI 763 GTGCTC G[AT]GC[AT]^C
1612 GAGCTC
BspEI 176 TCCGGA T^CCGGA
1094 TCCGGA
BspMI 433 ACCTGC ACCTGC(4/8)
529 ACCTGC
BsrDI 1627 CATTGC GCAATG(2/0)
1647 CATTGC
BstEII 1246 GGTCACC G^GT[ACGT]ACC
1288 GGTCACC
BstPI 1246 GGTCACC G^GT[ACGT]ACC
1288 GGTCACC
BstXI 638 CCAAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1727 CCAGCCAGCTGG
EcoO65I 1246 GGTCACC G^GT[ACGT]ACC
1288 GGTCACC
EcoT14I 140 CCATGG C^C[AT][AT]GG
1232 CCAAGG
MaeI 13 CTAG C^TAG
1694 CTAG
MaeII 623 ACGT A^CGT
761 ACGT
MroI 176 TCCGGA T^CCGGA
1094 TCCGGA
NspI 258 GCATGC [GA]CATG^[CT]
488 ACATGT
PflFI 1345 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1366 GACTCTGTC
PstI 662 CTGCAG CTGCA^G
1121 CTGCAG
SexAI 443 ACCTGGT A^CC[AT]GGT
1110 ACCTGGT
StyI 140 CCATGG C^C[AT][AT]GG
1232 CCAAGG
Tth111I 1345 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1366 GACTCTGTC
XcmI 140 CCATGGTCATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1440 CCATGTCTCTGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
BalI BanI BanII BbvI BcgI
BmyI BsaJI BseRI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII EaeI EcoRII Fnu4HI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NdeII NlaIII NlaIV PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TseI Tsp45I Tsp509I
TspRI XhoII