(Circular) (Six-base) MAPSORT of: pFN21AE9435.seq Check: 8595 from: 1 to: 6556 pFN21AE9435 6556 bp With 182 enzymes: SgfI * March 30, 2011 18:12 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2543 2543 Size: 6556 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4366 278 Size: 53 83 186 936 2830 2468 Fragments arranged by size: 2830 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2127 3315 3899 1937 Size: 190 1188 584 4594 Fragments arranged by size: 4594 1188 584 190 Acc65I G'GTAC_C Cuts at: 3877 3877 Size: 6556 AclI AA'CG_TT Cuts at: 1563 4683 5056 1563 Size: 3120 373 3063 Fragments arranged by size: 3120 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2407 3463 4619 6003 6271 1632 Size: 775 1056 1156 1384 268 1917 Fragments arranged by size: 1917 1384 1156 1056 775 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6359 AflIII A'CryG_T Cuts at: 1203 2493 5455 6432 1203 Size: 1290 2962 977 1327 Fragments arranged by size: 2962 1327 1290 977 AgeI A'CCGG_T Cuts at: 5387 5387 Size: 6556 AhdI GACnn_n'nnGTC Cuts at: 5285 5285 Size: 6556 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6556 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6556 AlwNI CAG_nnn'CTG Cuts at: 4299 5871 4299 Size: 1572 4984 ApaI G_GGCC'C Cuts at: 2013 3717 2013 Size: 1704 4852 ApaLI G'TGCA_C Cuts at: 4613 5769 4613 Size: 1156 5400 ApoI r'AATT_y Cuts at: 1493 2185 2902 3865 4091 4243 6352 1493 Size: 692 717 963 226 152 2109 1697 Fragments arranged by size: 2109 1697 963 717 692 226 152 AseI AT'TA_AT Cuts at: 160 1032 5110 160 Size: 872 4078 1606 Fragments arranged by size: 4078 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6556 AvaI C'yCGr_G Cuts at: 1870 1943 3638 3881 1870 Size: 73 1695 243 4545 Fragments arranged by size: 4545 1695 243 73 BamHI G'GATC_C Cuts at: 3886 4253 3886 Size: 367 6189 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2504 2754 2943 3877 5332 Size: 325 217 627 717 250 189 934 1455 Cuts at: 5332 618 Size: 1842 Fragments arranged by size: 1842 1455 934 717 627 325 250 217 189 BanII G_rGCy'C Cuts at: 1391 2013 2892 3549 3621 3717 3875 1391 Size: 622 879 657 72 96 158 4072 Fragments arranged by size: 4072 879 657 622 158 96 72 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2557 928 Size: 971 658 4927 Fragments arranged by size: 4927 971 658 BbvCI CC'TCA_GC Cuts at: 2697 2697 Size: 6556 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2182 2216 2575 2609 3629 3663 4747 Size: 34 245 34 359 34 1020 34 1084 Cuts at: 4747 4781 1903 Size: 34 3678 Fragments arranged by size: 3678 1084 1020 359 245 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4448 5658 4448 Size: 1210 5346 BclI T'GATC_A Cuts at: 1548 1548 Size: 6556 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5167 136 Size: 107 122 71 631 605 3495 1525 Fragments arranged by size: 3495 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6551 6551 Size: 6556 BlpI GC'TnA_GC Cuts at: 3543 3973 3543 Size: 430 6126 Bme1580I G_kGCm'C Cuts at: 1790 2013 2759 3025 3061 3717 4617 5773 1790 Size: 223 746 266 36 656 900 1156 2573 Fragments arranged by size: 2573 1156 900 746 656 266 223 36 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6556 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4287 5245 442 Size: 416 53 83 399 24 2870 958 1753 Fragments arranged by size: 2870 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6556 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3712 3712 Size: 6556 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2390 3761 5216 1239 Size: 1151 1371 1455 2579 Fragments arranged by size: 2579 1455 1371 1151 Bpu10I CC'TnA_GC Cuts at: 2697 3162 3321 3591 3615 2697 Size: 465 159 270 24 5638 Fragments arranged by size: 5638 465 270 159 24 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3242 4681 5546 5844 3242 Size: 1439 865 298 3954 Fragments arranged by size: 3954 1439 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5219 882 Size: 617 3720 2219 Fragments arranged by size: 3720 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2629 4347 493 Size: 711 1425 1718 2702 Fragments arranged by size: 2702 1718 1425 711 BsaBI GATnn'nnATC Cuts at: 2111 6550 2111 Size: 4439 2117 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4363 4745 5380 275 Size: 53 83 186 936 2830 382 635 1451 Fragments arranged by size: 2830 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2181 3099 4989 5387 5661 5808 6219 2181 Size: 918 1890 398 274 147 411 2518 Fragments arranged by size: 2518 1890 918 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6331 Fragments arranged by size: 6331 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2597 2726 2882 3172 1186 Size: 1411 129 156 290 4570 Fragments arranged by size: 4570 1411 290 156 129 BseYI C'CCAG_C Cuts at: 2061 2283 3710 5759 6255 2061 Size: 222 1427 2049 496 2362 Fragments arranged by size: 2362 2049 1427 496 222 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5983 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3145 4006 4767 5795 1540 Size: 275 186 1144 861 761 1028 2301 Fragments arranged by size: 2301 1144 1028 861 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2772 3621 4617 4702 5773 2772 Size: 849 996 85 1071 3555 Fragments arranged by size: 3555 1071 996 849 85 BsmI GAATG_Cn' Cuts at: 4067 4160 4067 Size: 93 6463 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2759 2772 2892 3025 3061 3549 Size: 399 223 746 13 120 133 36 488 Cuts at: 3549 3621 3717 3875 4617 4702 5773 1391 Size: 72 96 158 742 85 1071 2174 Fragments arranged by size: 2174 1071 746 742 488 399 223 158 133 120 96 85 72 36 13 BspEI T'CCGG_A Cuts at: 2181 3099 6219 2181 Size: 918 3120 2518 Fragments arranged by size: 3120 2518 918 BspHI T'CATG_A Cuts at: 2466 4445 6175 2466 Size: 1979 1730 2847 Fragments arranged by size: 2847 1979 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2447 2543 3911 844 Size: 1603 96 1368 3489 Fragments arranged by size: 3489 1603 1368 96 BsrBI CCG'CTC Cuts at: 1394 1823 4443 1394 Size: 429 2620 3507 Fragments arranged by size: 3507 2620 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3631 3651 5051 5225 65 Size: 1183 2383 20 1400 174 1396 Fragments arranged by size: 2383 1400 1396 1183 174 20 BsrFI r'CCGG_y Cuts at: 1700 1810 2077 5200 5387 1700 Size: 110 267 3123 187 2869 Fragments arranged by size: 3123 2869 267 187 110 BsrGI T'GTAC_A Cuts at: 96 2496 96 Size: 2400 4156 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6556 BssSI C'ACGA_G Cuts at: 3042 4616 5628 3042 Size: 1574 1012 3970 Fragments arranged by size: 3970 1574 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2439 794 Size: 1645 4911 BstBI TT'CG_AA Cuts at: 5360 5360 Size: 6556 BstEII G'GTnAC_C Cuts at: 1184 3251 3293 1184 Size: 2067 42 4447 Fragments arranged by size: 4447 2067 42 BstXI CCAn_nnnn'nTGG Cuts at: 2650 3739 2650 Size: 1089 5467 BstYI r'GATC_y Cuts at: 1972 2287 2418 2634 2814 3176 3642 3886 4253 Size: 315 131 216 180 362 466 244 367 Cuts at: 4253 4638 4655 6107 6193 6205 6551 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 466 385 367 362 346 315 244 216 180 131 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2145 513 Size: 552 939 141 4924 Fragments arranged by size: 4924 939 552 141 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2120 3739 487 Size: 683 56 894 1619 3304 Fragments arranged by size: 3304 1619 894 683 56 BtsI GCAGTG_nn' Cuts at: 2751 3552 3597 4072 4866 4886 2751 Size: 801 45 475 794 20 4421 Fragments arranged by size: 4421 801 794 475 45 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6556 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6521 DraI TTT'AAA Cuts at: 3860 3860 Size: 6556 DraIII CAC_nnn'GTG Cuts at: 3664 3664 Size: 6556 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5563 809 Size: 507 4247 1802 Fragments arranged by size: 4247 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2220 2235 3133 Size: 54 1425 50 275 18 390 15 898 Cuts at: 3133 3154 3271 3439 3514 4003 4892 6521 8 Size: 21 117 168 75 489 889 1629 43 Fragments arranged by size: 1629 1425 898 889 489 390 275 168 117 75 54 50 43 21 18 15 EagI C'GGCC_G Cuts at: 1537 1812 4003 1537 Size: 275 2191 4090 Fragments arranged by size: 4090 2191 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2905 4486 1353 Size: 1552 1581 3423 Fragments arranged by size: 3423 1581 1552 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5143 5517 5663 387 Size: 1064 138 3554 374 146 1280 Fragments arranged by size: 3554 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3619 3619 Size: 6556 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2390 2407 3463 3761 4619 5216 6003 Size: 393 758 17 1056 298 858 597 787 Cuts at: 6003 6271 1239 Size: 268 1524 Fragments arranged by size: 1524 1056 858 787 758 597 393 298 268 17 EcoO109I rG'GnC_Cy Cuts at: 2009 2009 Size: 6556 EcoRI G'AATT_C Cuts at: 2185 3865 4243 2185 Size: 1680 378 4498 Fragments arranged by size: 4498 1680 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3241 3493 3241 Size: 252 6304 FspI TGC'GCA Cuts at: 2432 5062 2432 Size: 2630 3926 HaeII r_GCGC'y Cuts at: 1678 5703 1678 Size: 4025 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3134 3166 5197 5229 5271 Size: 32 135 32 1562 32 2031 32 42 Cuts at: 5271 5303 1373 Size: 32 2626 Fragments arranged by size: 2626 2031 1562 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3900 4146 669 Size: 988 281 1962 246 3079 Fragments arranged by size: 3079 1962 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6556 HpaI GTT'AAC Cuts at: 4146 4146 Size: 6556 KpnI G_GTAC'C Cuts at: 3881 3881 Size: 6556 MfeI C'AATT_G Cuts at: 4155 4155 Size: 6556 MluI A'CGCG_T Cuts at: 6432 6432 Size: 6556 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5670 5854 1305 Size: 4365 184 2007 Fragments arranged by size: 4365 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2237 3135 3156 3273 3441 Size: 54 1425 343 405 898 21 117 168 Cuts at: 3441 3516 10 Size: 75 3050 Fragments arranged by size: 3050 1425 898 405 343 168 117 75 54 21 MslI CAynn'nnrTG Cuts at: 518 1166 2093 2201 2723 4514 4873 5032 518 Size: 648 927 108 522 1791 359 159 2042 Fragments arranged by size: 2042 1791 927 648 522 359 159 108 MspA1I CmG'CkG Cuts at: 1606 2865 3131 3710 3739 3972 4649 5797 6042 Size: 1259 266 579 29 233 677 1148 245 Cuts at: 6042 1606 Size: 2120 Fragments arranged by size: 2120 1259 1148 677 579 266 245 233 29 NcoI C'CATG_G Cuts at: 513 1065 2004 2145 513 Size: 552 939 141 4924 Fragments arranged by size: 4924 939 552 141 NdeI CA'TA_TG Cuts at: 387 387 Size: 6556 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6556 NotI GC'GGCC_GC Cuts at: 4003 4003 Size: 6556 NruI TCG'CGA Cuts at: 3033 3033 Size: 6556 NspI r_CATG'y Cuts at: 1132 1229 2267 2497 3914 5459 1132 Size: 97 1038 230 1417 1545 2229 Fragments arranged by size: 2229 1545 1417 1038 230 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6556 PciI A'CATG_T Cuts at: 2493 5455 2493 Size: 2962 3594 PflMI CCAn_nnn'nTGG Cuts at: 1108 3116 1108 Size: 2008 4548 PmeI GTTT'AAAC Cuts at: 3860 3860 Size: 6556 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4625 4657 6138 6170 4625 Size: 32 1481 32 5011 Fragments arranged by size: 5011 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6556 PsiI TTA'TAA Cuts at: 4126 4126 Size: 6556 PspOMI G'GGCC_C Cuts at: 2009 3713 2009 Size: 1704 4852 PstI C_TGCA'G Cuts at: 830 2671 3130 3908 830 Size: 1841 459 778 3478 Fragments arranged by size: 3478 1841 778 459 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6556 PvuII CAG'CTG Cuts at: 2865 3131 3739 2865 Size: 266 608 5682 Fragments arranged by size: 5682 608 266 SacI G_AGCT'C Cuts at: 3621 3621 Size: 6556 SalI G'TCGA_C Cuts at: 1936 3898 1936 Size: 1962 4594 SbfI CC_TGCA'GG Cuts at: 2671 3908 2671 Size: 1237 5319 ScaI AGT'ACT Cuts at: 4804 6516 4804 Size: 1712 4844 SexAI A'CCwGG_T Cuts at: 2448 3115 2448 Size: 667 5889 SfcI C'TryA_G Cuts at: 826 1045 2667 3126 3777 3904 5039 5720 5911 Size: 219 1622 459 651 127 1135 681 191 Cuts at: 5911 826 Size: 1471 Fragments arranged by size: 1622 1471 1135 681 651 459 219 191 127 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6556 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6556 SmaI CCC'GGG Cuts at: 1872 3883 1872 Size: 2011 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3257 4660 5561 5823 820 Size: 197 926 1314 1403 901 262 1553 Fragments arranged by size: 1553 1403 1314 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4347 493 Size: 3854 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6556 SphI G_CATG'C Cuts at: 1132 2267 3914 1132 Size: 1135 1647 3774 Fragments arranged by size: 3774 1647 1135 SspI AAT'ATT Cuts at: 5 52 4480 5 Size: 47 4428 2081 Fragments arranged by size: 4428 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6556 StyI C'CwwG_G Cuts at: 513 1065 2004 2145 3237 3995 513 Size: 552 939 141 1092 758 3074 Fragments arranged by size: 3074 1092 939 758 552 141 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4583 4600 4753 4938 5383 325 Size: 1255 3003 17 153 185 445 1498 Fragments arranged by size: 3003 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2496 4802 6514 Size: 274 80 33 51 1443 519 2306 1712 Cuts at: 6514 96 Size: 138 Fragments arranged by size: 2306 1712 1443 519 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2410 2442 6516 6548 2410 Size: 32 4074 32 2418 Fragments arranged by size: 4074 2418 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3353 3374 1316 Size: 2037 21 4498 Fragments arranged by size: 4498 2037 21 XbaI T'CTAG_A Cuts at: 3892 3892 Size: 6556 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2152 3452 2152 Size: 1300 5256 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6556 XmaI C'CCGG_G Cuts at: 1870 3881 1870 Size: 2011 4545 XmnI GAAnn'nnTTC Cuts at: 4685 4685 Size: 6556 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4364 276 Size: 53 83 186 936 2830 2468 Fragments arranged by size: 2830 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI