(Linear) (Six-base) MAPSORT of: KIEE9435.seq Check: 8992 from: 1 to: 1851 KIEE9435.orf With 182 enzymes: SgfI * March 30, 2011 17:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 538 1851 Size: 538 1313 AccI GT'mk_AC Cuts at: 0 122 1310 1851 Size: 122 1188 541 Fragments arranged by size: 1188 541 122 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 402 1458 1851 Size: 402 1056 393 Fragments arranged by size: 1056 402 393 AflIII A'CryG_T Cuts at: 0 488 1851 Size: 488 1363 ApaI G_GGCC'C Cuts at: 0 8 1712 1851 Size: 8 1704 139 Fragments arranged by size: 1704 139 8 ApoI r'AATT_y Cuts at: 0 180 897 1851 Size: 180 717 954 Fragments arranged by size: 954 717 180 AvaI C'yCGr_G Cuts at: 0 1633 1851 Size: 1633 218 BanI G'GyrC_C Cuts at: 0 499 749 938 1851 Size: 499 250 189 913 Fragments arranged by size: 913 499 250 189 BanII G_rGCy'C Cuts at: 0 8 887 1544 1616 1712 1851 Size: 8 879 657 72 96 139 Fragments arranged by size: 879 657 139 96 72 8 BbsI GAAGACnn'nnnn_ Cuts at: 0 552 1851 Size: 552 1299 BbvCI CC'TCA_GC Cuts at: 0 692 1851 Size: 692 1159 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 177 211 570 604 1624 1658 1851 Size: 177 34 359 34 1020 34 193 Fragments arranged by size: 1020 359 193 177 34 34 34 BlpI GC'TnA_GC Cuts at: 0 1538 1851 Size: 1538 313 Bme1580I G_kGCm'C Cuts at: 0 8 754 1020 1056 1712 1851 Size: 8 746 266 36 656 139 Fragments arranged by size: 746 656 266 139 36 8 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1707 1851 Size: 1707 144 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 385 1756 1851 Size: 385 1371 95 Fragments arranged by size: 1371 385 95 Bpu10I CC'TnA_GC Cuts at: 0 692 1157 1316 1586 1610 1851 Size: 692 465 159 270 24 241 Fragments arranged by size: 692 465 270 241 159 24 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1237 1851 Size: 1237 614 BsaAI yAC'GTr Cuts at: 0 624 1851 Size: 624 1227 BsaBI GATnn'nnATC Cuts at: 0 106 1851 Size: 106 1745 BsaWI w'CCGG_w Cuts at: 0 176 1094 1851 Size: 176 918 757 Fragments arranged by size: 918 757 176 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 592 721 877 1167 1851 Size: 592 129 156 290 684 Fragments arranged by size: 684 592 290 156 129 BseYI C'CCAG_C Cuts at: 0 56 278 1705 1851 Size: 56 222 1427 146 Fragments arranged by size: 1427 222 146 56 BsiEI CG_ry'CG Cuts at: 0 1140 1851 Size: 1140 711 BsiHKAI G_wGCw'C Cuts at: 0 767 1616 1851 Size: 767 849 235 Fragments arranged by size: 849 767 235 Bsp1286I G_dGCh'C Cuts at: 0 8 754 767 887 1020 1056 1544 1616 Size: 8 746 13 120 133 36 488 72 Cuts at: 1616 1712 1851 Size: 96 139 Fragments arranged by size: 746 488 139 133 120 96 72 36 13 8 BspEI T'CCGG_A Cuts at: 0 176 1094 1851 Size: 176 918 757 Fragments arranged by size: 918 757 176 BspHI T'CATG_A Cuts at: 0 461 1851 Size: 461 1390 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 442 538 1851 Size: 442 96 1313 Fragments arranged by size: 1313 442 96 BsrDI GCAATG_nn' Cuts at: 0 1626 1646 1851 Size: 1626 20 205 Fragments arranged by size: 1626 205 20 BsrFI r'CCGG_y Cuts at: 0 72 1851 Size: 72 1779 BsrGI T'GTAC_A Cuts at: 0 491 1851 Size: 491 1360 BssSI C'ACGA_G Cuts at: 0 1037 1851 Size: 1037 814 BstAPI GCAn_nnn'nTGC Cuts at: 0 434 1851 Size: 434 1417 BstEII G'GTnAC_C Cuts at: 0 1246 1288 1851 Size: 1246 42 563 Fragments arranged by size: 1246 563 42 BstXI CCAn_nnnn'nTGG Cuts at: 0 645 1734 1851 Size: 645 1089 117 Fragments arranged by size: 1089 645 117 BstYI r'GATC_y Cuts at: 0 282 413 629 809 1171 1637 1851 Size: 282 131 216 180 362 466 214 Fragments arranged by size: 466 362 282 216 214 180 131 BtgI C'CryG_G Cuts at: 0 140 1851 Size: 140 1711 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 115 1734 1851 Size: 115 1619 117 Fragments arranged by size: 1619 117 115 BtsI GCAGTG_nn' Cuts at: 0 746 1547 1592 1851 Size: 746 801 45 259 Fragments arranged by size: 801 746 259 45 DraIII CAC_nnn'GTG Cuts at: 0 1659 1851 Size: 1659 192 EaeI y'GGCC_r Cuts at: 0 215 230 1128 1149 1266 1434 1509 1851 Size: 215 15 898 21 117 168 75 342 Fragments arranged by size: 898 342 215 168 117 75 21 15 EarI CTCTTCn'nnn_ Cuts at: 0 900 1851 Size: 900 951 EcoICRI GAG'CTC Cuts at: 0 1614 1851 Size: 1614 237 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 385 402 1458 1756 1851 Size: 385 17 1056 298 95 Fragments arranged by size: 1056 385 298 95 17 EcoO109I rG'GnC_Cy Cuts at: 0 4 1851 Size: 4 1847 EcoRI G'AATT_C Cuts at: 0 180 1851 Size: 180 1671 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1236 1488 1851 Size: 1236 252 363 Fragments arranged by size: 1236 363 252 FspI TGC'GCA Cuts at: 0 427 1851 Size: 427 1424 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1129 1161 1851 Size: 1129 32 690 Fragments arranged by size: 1129 690 32 MscI TGG'CCA Cuts at: 0 232 1130 1151 1268 1436 1511 1851 Size: 232 898 21 117 168 75 340 Fragments arranged by size: 898 340 232 168 117 75 21 MslI CAynn'nnrTG Cuts at: 0 88 196 718 1851 Size: 88 108 522 1133 Fragments arranged by size: 1133 522 108 88 MspA1I CmG'CkG Cuts at: 0 860 1126 1705 1734 1851 Size: 860 266 579 29 117 Fragments arranged by size: 860 579 266 117 29 NcoI C'CATG_G Cuts at: 0 140 1851 Size: 140 1711 NruI TCG'CGA Cuts at: 0 1028 1851 Size: 1028 823 NspI r_CATG'y Cuts at: 0 262 492 1851 Size: 262 230 1359 Fragments arranged by size: 1359 262 230 PciI A'CATG_T Cuts at: 0 488 1851 Size: 488 1363 PflMI CCAn_nnn'nTGG Cuts at: 0 1111 1851 Size: 1111 740 PspOMI G'GGCC_C Cuts at: 0 4 1708 1851 Size: 4 1704 143 Fragments arranged by size: 1704 143 4 PstI C_TGCA'G Cuts at: 0 666 1125 1851 Size: 666 459 726 Fragments arranged by size: 726 666 459 PvuII CAG'CTG Cuts at: 0 860 1126 1734 1851 Size: 860 266 608 117 Fragments arranged by size: 860 608 266 117 SacI G_AGCT'C Cuts at: 0 1616 1851 Size: 1616 235 SbfI CC_TGCA'GG Cuts at: 0 666 1851 Size: 666 1185 SexAI A'CCwGG_T Cuts at: 0 443 1110 1851 Size: 443 667 741 Fragments arranged by size: 741 667 443 SfcI C'TryA_G Cuts at: 0 662 1121 1772 1851 Size: 662 459 651 79 Fragments arranged by size: 662 651 459 79 SmlI C'TyrA_G Cuts at: 0 1252 1851 Size: 1252 599 SphI G_CATG'C Cuts at: 0 262 1851 Size: 262 1589 StyI C'CwwG_G Cuts at: 0 140 1232 1851 Size: 140 1092 619 Fragments arranged by size: 1092 619 140 TatI w'GTAC_w Cuts at: 0 491 1851 Size: 491 1360 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 405 437 1851 Size: 405 32 1414 Fragments arranged by size: 1414 405 32 Tth111I GACn'n_nGTC Cuts at: 0 1348 1369 1851 Size: 1348 21 482 Fragments arranged by size: 1348 482 21 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 147 1447 1851 Size: 147 1300 404 Fragments arranged by size: 1300 404 147 Enzymes that do cut: AarI AccI AcuI AflIII ApaI ApoI AvaI BanI BanII BbsI BbvCI BcgI BlpI Bme1580I BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI BtgI BtgZI BtsI DraIII EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI Hin4I MscI MslI MspA1I NcoI NruI NspI PciI PflMI PspOMI PstI PvuII SacI SbfI SexAI SfcI SmlI SphI StyI TatI TstI Tth111I XcmI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI AlwNI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BciVI BclI BfrBI BglI BglII BmgBI BmrI BmtI BsaI BsaHI BsaXI BsgI BsiWI BsmI BsmBI BsrBI BssHII BstBI BstZ17I Bsu36I ClaI CspCI DraI DrdI EagI EciI EcoNI EcoRV FseI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI NsiI PacI PasI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI TaqII XbaI XhoI XmaI XmnI ZraI