(Circular) (Six-base) MAPSORT of: pFN21AB9863.seq Check: 4196 from: 1 to: 5956 pFN21AB9863 5956 bp With 182 enzymes: SgfI * March 28, 2012 12:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2173 2655 3074 2173 Size: 482 419 5055 Fragments arranged by size: 5055 482 419 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3766 278 Size: 53 83 186 936 2230 2468 Fragments arranged by size: 2468 2230 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3299 1937 Size: 1362 4594 Acc65I G'GTAC_C Cuts at: 3277 3277 Size: 5956 AclI AA'CG_TT Cuts at: 1563 4083 4456 1563 Size: 2520 373 3063 Fragments arranged by size: 3063 2520 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2325 4019 5403 5671 1632 Size: 693 1694 1384 268 1917 Fragments arranged by size: 1917 1694 1384 693 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5759 AflIII A'CryG_T Cuts at: 1203 2574 4855 5832 1203 Size: 1371 2281 977 1327 Fragments arranged by size: 2281 1371 1327 977 AgeI A'CCGG_T Cuts at: 4787 4787 Size: 5956 AhdI GACnn_n'nnGTC Cuts at: 4685 4685 Size: 5956 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5956 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5956 AlwNI CAG_nnn'CTG Cuts at: 3025 3699 5271 3025 Size: 674 1572 3710 Fragments arranged by size: 3710 1572 674 ApaI G_GGCC'C Cuts at: 2722 2722 Size: 5956 ApaLI G'TGCA_C Cuts at: 4013 5169 4013 Size: 1156 4800 ApoI r'AATT_y Cuts at: 1493 3033 3265 3491 3643 5752 1493 Size: 1540 232 226 152 2109 1697 Fragments arranged by size: 2109 1697 1540 232 226 152 AseI AT'TA_AT Cuts at: 160 1032 4510 160 Size: 872 3478 1606 Fragments arranged by size: 3478 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5956 AvaI C'yCGr_G Cuts at: 1870 1943 2214 2242 2635 2743 2768 3281 1870 Size: 73 271 28 393 108 25 513 4545 Fragments arranged by size: 4545 513 393 271 108 73 28 25 BamHI G'GATC_C Cuts at: 3286 3653 3286 Size: 367 5589 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2264 2278 2845 2884 3230 Size: 325 217 627 477 14 567 39 346 Cuts at: 3230 3277 4732 618 Size: 47 1455 1842 Fragments arranged by size: 1842 1455 627 567 477 346 325 217 47 39 14 BanII G_rGCy'C Cuts at: 1391 2644 2722 3275 1391 Size: 1253 78 553 4072 Fragments arranged by size: 4072 1253 553 78 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2079 2118 2130 928 Size: 971 180 39 12 4754 Fragments arranged by size: 4754 971 180 39 12 BbvCI CC'TCA_GC Cuts at: 2918 2918 Size: 5956 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2789 2823 4147 4181 1903 Size: 34 852 34 1324 34 3678 Fragments arranged by size: 3678 1324 852 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3848 5058 3848 Size: 1210 4746 BclI T'GATC_A Cuts at: 1548 1548 Size: 5956 BfrBI ATG'CAT Cuts at: 2969 2969 Size: 5956 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2532 3247 4567 Size: 107 122 71 631 605 860 715 1320 Cuts at: 4567 136 Size: 1525 Fragments arranged by size: 1525 1320 860 715 631 605 122 107 71 BglII A'GATC_T Cuts at: 5951 5951 Size: 5956 BlpI GC'TnA_GC Cuts at: 2022 2396 3373 2022 Size: 374 977 4605 Fragments arranged by size: 4605 977 374 Bme1580I G_kGCm'C Cuts at: 1790 2281 2722 2848 3233 4017 5173 1790 Size: 491 441 126 385 784 1156 2573 Fragments arranged by size: 2573 1156 784 491 441 385 126 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5956 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3687 4645 442 Size: 416 53 83 399 24 2270 958 1753 Fragments arranged by size: 2270 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5956 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2153 2569 3055 4616 1239 Size: 914 416 486 1561 2579 Fragments arranged by size: 2579 1561 914 486 416 Bpu10I CC'TnA_GC Cuts at: 2918 2918 Size: 5956 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4081 4946 5244 4081 Size: 865 298 4793 Fragments arranged by size: 4793 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4619 882 Size: 617 3120 2219 Fragments arranged by size: 3120 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3747 493 Size: 711 2543 2702 Fragments arranged by size: 2702 2543 711 BsaBI GATnn'nnATC Cuts at: 5950 5950 Size: 5956 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2776 2947 3763 4145 Size: 53 83 186 936 1243 171 816 382 Cuts at: 4145 4780 275 Size: 635 1451 Fragments arranged by size: 1451 1243 936 816 635 382 186 171 83 53 BsaWI w'CCGG_w Cuts at: 4389 4787 5061 5208 5619 4389 Size: 398 274 147 411 4726 Fragments arranged by size: 4726 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2549 2579 1178 Size: 30 165 30 1146 30 4555 Fragments arranged by size: 4555 1146 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2114 2144 2147 2944 3193 1186 Size: 928 30 3 797 249 3949 Fragments arranged by size: 3949 928 797 249 30 3 BseYI C'CCAG_C Cuts at: 3222 5159 5655 3222 Size: 1937 496 3523 Fragments arranged by size: 3523 1937 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2658 2672 2777 1163 Size: 573 922 14 105 4342 Fragments arranged by size: 4342 922 573 105 14 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3047 3406 4167 5195 1540 Size: 275 186 1046 359 761 1028 2301 Fragments arranged by size: 2301 1046 1028 761 359 275 186 BsiHKAI G_wGCw'C Cuts at: 2223 4017 4102 5173 2223 Size: 1794 85 1071 3006 Fragments arranged by size: 3006 1794 1071 85 BsmI GAATG_Cn' Cuts at: 3467 3560 3467 Size: 93 5863 BsmBI CGTCTCn'nnnn_ Cuts at: 2767 2767 Size: 5956 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2223 2281 2644 2722 2848 3233 3275 Size: 399 433 58 363 78 126 385 42 Cuts at: 3275 4017 4102 5173 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 433 399 385 363 126 85 78 58 42 BspEI T'CCGG_A Cuts at: 5619 5619 Size: 5956 BspHI T'CATG_A Cuts at: 3845 5575 3845 Size: 1730 4226 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2019 2173 2655 2677 3074 3311 844 Size: 1175 154 482 22 397 237 3489 Fragments arranged by size: 3489 1175 482 397 237 154 22 BsrBI CCG'CTC Cuts at: 1394 1823 3013 3843 1394 Size: 429 1190 830 3507 Fragments arranged by size: 3507 1190 830 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4451 4625 65 Size: 1183 3203 174 1396 Fragments arranged by size: 3203 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2320 4600 4787 1700 Size: 110 510 2280 187 2869 Fragments arranged by size: 2869 2280 510 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5956 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5956 BssSI C'ACGA_G Cuts at: 4016 5028 4016 Size: 1012 4944 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5956 BstBI TT'CG_AA Cuts at: 4760 4760 Size: 5956 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5956 BstYI r'GATC_y Cuts at: 1972 2508 3286 3653 4038 4055 5507 5593 5605 Size: 536 778 367 385 17 1452 86 12 Cuts at: 5605 5951 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 778 536 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2358 513 Size: 552 1293 4111 Fragments arranged by size: 4111 1293 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2329 487 Size: 683 56 1103 4114 Fragments arranged by size: 4114 1103 683 56 BtsI GCAGTG_nn' Cuts at: 3472 4266 4286 3472 Size: 794 20 5142 Fragments arranged by size: 5142 794 20 ClaI AT'CG_AT Cuts at: 1554 2085 1554 Size: 531 5425 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5921 DraI TTT'AAA Cuts at: 3260 3260 Size: 5956 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4963 809 Size: 507 3647 1802 Fragments arranged by size: 3647 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2361 2422 3403 Size: 54 1425 50 275 18 531 61 981 Cuts at: 3403 4292 5921 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 981 889 531 275 61 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3403 1537 Size: 275 1591 4090 Fragments arranged by size: 4090 1591 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2811 3886 1353 Size: 1458 1075 3423 Fragments arranged by size: 3423 1458 1075 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2462 2978 3117 4543 4917 5063 Size: 1064 138 873 516 139 1426 374 146 Cuts at: 5063 387 Size: 1280 Fragments arranged by size: 1426 1280 1064 873 516 374 146 139 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2153 2325 2569 3055 4019 4616 5403 Size: 393 521 172 244 486 964 597 787 Cuts at: 5403 5671 1239 Size: 268 1524 Fragments arranged by size: 1524 964 787 597 521 486 393 268 244 172 EcoNI CCTnn'n_nnAGG Cuts at: 2096 2096 Size: 5956 EcoO109I rG'GnC_Cy Cuts at: 2705 2718 2719 2748 2705 Size: 13 1 29 5913 Fragments arranged by size: 5913 29 13 1 EcoRI G'AATT_C Cuts at: 3265 3643 3265 Size: 378 5578 FspI TGC'GCA Cuts at: 3029 4462 3029 Size: 1433 4523 HaeII r_GCGC'y Cuts at: 1678 2029 2389 2623 5103 1678 Size: 351 360 234 2480 2531 Fragments arranged by size: 2531 2480 360 351 234 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2208 2240 2549 2581 4597 Size: 32 135 32 636 32 309 32 2016 Cuts at: 4597 4629 4671 4703 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2016 636 309 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3300 3546 669 Size: 988 281 1362 246 3079 Fragments arranged by size: 3079 1362 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5956 HpaI GTT'AAC Cuts at: 3546 3546 Size: 5956 KpnI G_GTAC'C Cuts at: 3281 3281 Size: 5956 MfeI C'AATT_G Cuts at: 3555 3555 Size: 5956 MluI A'CGCG_T Cuts at: 5832 5832 Size: 5956 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5070 5254 1305 Size: 3765 184 2007 Fragments arranged by size: 3765 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2363 10 Size: 54 1425 343 531 3603 Fragments arranged by size: 3603 1425 531 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2357 3914 4273 4432 518 Size: 648 1191 1557 359 159 2042 Fragments arranged by size: 2042 1557 1191 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2400 3106 3139 3372 4049 5197 5442 1606 Size: 794 706 33 233 677 1148 245 2120 Fragments arranged by size: 2120 1148 794 706 677 245 233 33 NaeI GCC'GGC Cuts at: 2322 2322 Size: 5956 NcoI C'CATG_G Cuts at: 513 1065 2358 513 Size: 552 1293 4111 Fragments arranged by size: 4111 1293 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5956 NgoMIV G'CCGG_C Cuts at: 2320 2320 Size: 5956 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5956 NotI GC'GGCC_GC Cuts at: 3403 3403 Size: 5956 NsiI A_TGCA'T Cuts at: 2971 2971 Size: 5956 NspI r_CATG'y Cuts at: 1132 1229 2578 3314 4859 1132 Size: 97 1349 736 1545 2229 Fragments arranged by size: 2229 1545 1349 736 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5956 PciI A'CATG_T Cuts at: 2574 4855 2574 Size: 2281 3675 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5956 PfoI T'CCnGG_A Cuts at: 2055 2055 Size: 5956 PmeI GTTT'AAAC Cuts at: 3260 3260 Size: 5956 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4025 4057 5538 5570 4025 Size: 32 1481 32 4411 Fragments arranged by size: 4411 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5956 PsiI TTA'TAA Cuts at: 3526 3526 Size: 5956 PspOMI G'GGCC_C Cuts at: 2718 2718 Size: 5956 PstI C_TGCA'G Cuts at: 830 2184 2903 3308 830 Size: 1354 719 405 3478 Fragments arranged by size: 3478 1354 719 405 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5956 SalI G'TCGA_C Cuts at: 1936 3298 1936 Size: 1362 4594 SbfI CC_TGCA'GG Cuts at: 3308 3308 Size: 5956 ScaI AGT'ACT Cuts at: 4204 5916 4204 Size: 1712 4244 SfcI C'TryA_G Cuts at: 826 1045 2180 2899 3304 4439 5120 5311 826 Size: 219 1135 719 405 1135 681 191 1471 Fragments arranged by size: 1471 1135 1135 719 681 405 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3247 3247 Size: 5956 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5956 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5956 SmaI CCC'GGG Cuts at: 1872 2637 2745 2770 3283 1872 Size: 765 108 25 513 4545 Fragments arranged by size: 4545 765 513 108 25 SmlI C'TyrA_G Cuts at: 820 1017 1943 4060 4961 5223 820 Size: 197 926 2117 901 262 1553 Fragments arranged by size: 2117 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3747 493 Size: 3254 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5956 SphI G_CATG'C Cuts at: 1132 3314 1132 Size: 2182 3774 SspI AAT'ATT Cuts at: 5 52 3880 5 Size: 47 3828 2081 Fragments arranged by size: 3828 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5956 StyI C'CwwG_G Cuts at: 513 1065 2285 2358 2923 3209 3395 513 Size: 552 1220 73 565 286 186 3074 Fragments arranged by size: 3074 1220 565 552 286 186 73 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2544 3983 4000 4153 4338 4783 325 Size: 1255 964 1439 17 153 185 445 1498 Fragments arranged by size: 1498 1439 1255 964 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4202 5914 96 Size: 274 80 33 51 1443 2225 1712 138 Fragments arranged by size: 2225 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5916 5948 5916 Size: 32 5924 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5956 XbaI T'CTAG_A Cuts at: 3292 3292 Size: 5956 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5956 XmaI C'CCGG_G Cuts at: 1870 2635 2743 2768 3281 1870 Size: 765 108 25 513 4545 Fragments arranged by size: 4545 765 513 108 25 XmnI GAAnn'nnTTC Cuts at: 2612 4085 2612 Size: 1473 4483 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3764 276 Size: 53 83 186 936 2230 2468 Fragments arranged by size: 2468 2230 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfoI SrfI SwaI XcmI