################################## # NO CUT ENZYMES ( 130 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AvrII BamHI BbeI BbrPI BciVI BclI BfrI BglII BlnI BsaAI BsaBI BsaI BsaWI BseAI BsiWI BsmI Bsp1407I BspEI BspHI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV FbaI FseI HincII HindII HindIII HpaI KasI KpnI KspI MaeII MaeIII MamI MfeI MluI MroI MunI NarI NdeI NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tsp45I TspRI Tth111I Van91I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AflIII 569 ACATGT A^C[GA][CT]GT AlwI 504 GATCC GGATC(4/5) AlwNI 1015 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 713 GGGCCC GGGCC^C Asp700I 603 GAAGCGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 214 GAGCAC G[AT]GC[AT]^C AviII 1022 TGCGCA TGC^GCA BalI 356 TGGCCA TGG^CCA BbvCI 912 CCTCAGC CCTCAGC(-5/-2) BcgI 795 GCATCCTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 151 CTAG C^TAG BsiEI 1039 CGACCG CG[GA][CT]^CG BsiHKAI 214 GAGCAC G[AT]GC[AT]^C BsmAI 768 GAGAC GTCTC(1/5) BsmBI 768 GAGACG CGTCTC(1/5) BspDI 79 ATCGAT AT^CGAT BspLU11I 569 ACATGT A^CATGT BsrBI 1006 GAGCGG CCGCTC(-3/-3) BsrFI 315 GCCGGC [GA]^CCGG[CT] BstYI 503 AGATCC [GA]^GATC[CT] Cfr10I 315 GCCGGC [GA]^CCGG[CT] ClaI 79 ATCGAT AT^CGAT DpnII 504 GATC ^GATC DsaI 353 CCATGG C^C[GA][CT]GG EarI 800 CTCTTC CTCTTC(1/4) EcoNI 87 CCTGGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 962 ATGCAT ATGCA^T FspI 1022 TGCGCA TGC^GCA HinfI 55 GACTC G^A[ACGT]TC HphI 350 TCACC GGTGA(8/7) Ksp632I 800 CTCTTC CTCTTC(1/4) MaeI 151 CTAG C^TAG MboI 504 GATC ^GATC MflI 503 AGATCC [GA]^GATC[CT] MluNI 356 TGGCCA TGG^CCA MscI 356 TGGCCA TGG^CCA MseI 500 TTAA T^TAA MslI 348 CATCACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 315 GCCGGC GCC^GGC NcoI 353 CCATGG C^CATGG NdeII 504 GATC ^GATC NgoMIV 315 GCCGGC G^CCGGC NsiI 962 ATGCAT ATGCA^T NspI 569 ACATGT [GA]CATG^[CT] PleI 55 GACTC GAGTC(4/5) Sau3AI 504 GATC ^GATC Tru9I 500 TTAA T^TAA Tsp509I 1145 AATT ^AATT XhoII 503 AGATCC [GA]^GATC[CT] XmnI 603 GAAGCGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AcyI 770 GACGCC G[GA]^CG[CT]C 941 GGCGTC AfaI 336 GTAC GT^AC 1012 GTAC AvaII 552 GGACC G^G[AT]CC 909 GGTCC BanII 635 GAGCCC G[GA]GC[CT]^C 713 GGGCCC BglI 521 GCCACCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1236 GCCTCGTCGGC BlpI 16 GCTGAGC GC^T[ACGT]AGC 390 GCTCAGC Bpu1102I 16 GCTGAGC GC^T[ACGT]AGC 390 GCTCAGC BsaHI 770 GACGCC G[GA]^CG[CT]C 941 GGCGTC BsrI 542 ACTGG ACTGG(1/-1) 733 CCAGT CelII 16 GCTGAGC GC^T[ACGT]AGC 390 GCTCAGC EaeI 356 TGGCCA [CT]^GGCC[GA] 417 TGGCCG HgaI 770 GACGC GACGC(5/10) 942 GCGTC Hin1I 770 GACGCC G[GA]^CG[CT]C 941 GGCGTC NlaIII 354 CATG CATG^ 570 CATG PstI 175 CTGCAG CTGCA^G 894 CTGCAG RsaI 336 GTAC GT^AC 1012 GTAC SfcI 175 CTGCAG C^T[GA][CT]AG 894 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 61 enzymes) # ######################################### AccII AciI AluI AvaI BanI BbsI BbvI BcnI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiYI BslI BsmFI BsoBI Bsp1286I BspMI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DraII EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HpaII ItaI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIV Sau96I ScrFI SfaNI SmaI StyI TaqI TseI TthHB8I XmaCI XmaI