################################## # NO CUT ENZYMES ( 140 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AflII AgeI AhdI AlwNI Aor51HI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbrPI BbvCI BbvI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BsgI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV FauI FbaI FseI FspI HgaI KpnI KspI MaeI MamI MfeI MluI MroI MspA1I MunI MvnI MwoI NaeI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI TseI Tsp45I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 61 enzymes) # ################################### AciI 696 CCGC CCGC(-3/-1) AcyI 892 GGCGCC G[GA]^CG[CT]C AflIII 206 ACATGT A^C[GA][CT]GT Alw44I 417 GTGCAC G^TGCAC AocI 870 CCTGAGG CC^T[ACGT]AGG ApaI 505 GGGCCC GGGCC^C ApaLI 417 GTGCAC G^TGCAC BanI 892 GGCGCC G^G[CT][GA]CC BanII 505 GGGCCC G[GA]GC[CT]^C BbeI 892 GGCGCC GGCGC^C BbsI 94 GTCTTC GAAGAC(2/6) BpuAI 94 GTCTTC GAAGAC(2/6) BsaHI 892 GGCGCC G[GA]^CG[CT]C BsaI 642 GGTCTC GGTCTC(1/5) BsmAI 643 GTCTC GTCTC(1/5) BsmI 876 GAATGC GAATGC(1/-1) BspHI 401 TCATGA T^CATGA BspLU11I 206 ACATGT A^CATGT BspMI 715 ACCTGC ACCTGC(4/8) BstXI 233 CCAAGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 677 AGATCC [GA]^GATC[CT] Bsu36I 870 CCTGAGG CC^T[ACGT]AGG Cac8I 555 GCTGGC GC[ACGT]^[ACGT]GC CfoI 893 GCGC GCG^C DraII 505 GGGCCCC [GA]G^G[ACGT]CC[CT] DsaI 761 CCATGG C^C[GA][CT]GG Eco81I 870 CCTGAGG CC^T[ACGT]AGG EcoNI 353 CCTATGATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 505 GGGCCCC [GA]G^G[ACGT]CC[CT] EcoT14I 761 CCATGG C^C[AT][AT]GG EcoT22I 285 ATGCAT ATGCA^T Fnu4HI 695 GCCGC GC^[ACGT]GC HaeII 892 GGCGCC [GA]GCGC^[CT] HapII 510 CCGG C^CGG HhaI 893 GCGC GCG^C Hin1I 892 GGCGCC G[GA]^CG[CT]C HinP1I 893 GCGC G^CGC HincII 577 GTTAAC GT[CT]^[GA]AC HindII 577 GTTAAC GT[CT]^[GA]AC HindIII 157 AAGCTT A^AGCTT HpaI 577 GTTAAC GTT^AAC HpaII 510 CCGG C^CGG ItaI 695 GCCGC GC^[ACGT]GC KasI 892 GGCGCC G^GCGCC MaeII 436 ACGT A^CGT MaeIII 322 GTTAC ^GT[ACGT]AC MflI 677 AGATCC [GA]^GATC[CT] MspI 510 CCGG C^CGG NarI 892 GGCGCC GG^CGCC NcoI 761 CCATGG C^CATGG NsiI 285 ATGCAT ATGCA^T RcaI 401 TCATGA T^CATGA SfcI 864 CTACAG C^T[GA][CT]AG SfoI 892 GGCGCC GGC^GCC SmaI 509 CCCGGG CCC^GGG SmlI 550 CTCAAG C^T[CT][GA]AG StuI 704 AGGCCT AGG^CCT StyI 761 CCATGG C^C[AT][AT]GG XhoII 677 AGATCC [GA]^GATC[CT] XmaCI 509 CCCGGG C^CCGGG XmaI 509 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AcsI 476 AAATTC [GA]^AATT[CT] 536 AAATTC ApoI 476 AAATTC [GA]^AATT[CT] 536 AAATTC AspHI 417 GTGCAC G[AT]GC[AT]^C 905 GAGCAC AvaI 509 CCCGGG C^[CT]CG[GA]G 661 CTCGGG BalI 194 TGGCCA TGG^CCA 368 TGGCCA BcnI 509 CCCGG CC^[GC]GG 510 CCGGG BpmI 910 CTCCAG CTGGAG(16/14) 408 CTGGAG BsiHKAI 417 GTGCAC G[AT]GC[AT]^C 905 GAGCAC BsoBI 509 CCCGGG C^[CT]CG[GA]G 661 CTCGGG BsrI 63 ACTGG ACTGG(1/-1) 387 CCAGT EaeI 194 TGGCCA [CT]^GGCC[GA] 368 TGGCCA MluNI 194 TGGCCA TGG^CCA 368 TGGCCA MscI 194 TGGCCA TGG^CCA 368 TGGCCA MseI 578 TTAA T^TAA 839 TTAA NciI 509 CCCGG CC^[GC]GG 510 CCGGG NspI 206 ACATGT [GA]CATG^[CT] 650 ACATGC Tru9I 578 TTAA T^TAA 839 TTAA Tsp509I 477 AATT ^AATT 537 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AfaI AluI AlwI AvaII BmyI BsaJI BseRI BsiYI BslI BsmFI Bsp1286I BssKI BstF5I BstNI Cfr13I DdeI DpnII EarI EcoRII FokI HaeIII HinfI HphI Ksp632I MboI MboII MnlI MslI MvaI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI TfiI TspRI