(Circular) (Six-base) MAPSORT of: pFN21AE6053.seq Check: 490 from: 1 to: 5659 pFN21AE6053 5659 bp With 182 enzymes: SgfI * December 8, 2014 13:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2729 2729 Size: 5659 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3469 278 Size: 53 83 186 936 1933 2468 Fragments arranged by size: 2468 1933 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3002 1937 Size: 1065 4594 Acc65I G'GTAC_C Cuts at: 2980 2980 Size: 5659 AclI AA'CG_TT Cuts at: 1563 3786 4159 1563 Size: 2223 373 3063 Fragments arranged by size: 3063 2223 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2699 3722 5106 5374 1632 Size: 1067 1023 1384 268 1917 Fragments arranged by size: 1917 1384 1067 1023 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5462 AflIII A'CryG_T Cuts at: 1203 2211 4558 5535 1203 Size: 1008 2347 977 1327 Fragments arranged by size: 2347 1327 1008 977 AgeI A'CCGG_T Cuts at: 4490 4490 Size: 5659 AhdI GACnn_n'nnGTC Cuts at: 4388 4388 Size: 5659 AleI CACnn'nnGTG Cuts at: 1166 2736 1166 Size: 1570 4089 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5659 AlwNI CAG_nnn'CTG Cuts at: 3402 4974 3402 Size: 1572 4087 ApaI G_GGCC'C Cuts at: 2514 2514 Size: 5659 ApaLI G'TGCA_C Cuts at: 2422 3716 4872 2422 Size: 1294 1156 3209 Fragments arranged by size: 3209 1294 1156 ApoI r'AATT_y Cuts at: 1493 2481 2541 2968 3194 3346 5455 1493 Size: 988 60 427 226 152 2109 1697 Fragments arranged by size: 2109 1697 988 427 226 152 60 AseI AT'TA_AT Cuts at: 160 1032 4213 160 Size: 872 3181 1606 Fragments arranged by size: 3181 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5659 AvaI C'yCGr_G Cuts at: 1870 1943 2514 2666 2984 1870 Size: 73 571 152 318 4545 Fragments arranged by size: 4545 571 318 152 73 BamHI G'GATC_C Cuts at: 2989 3356 2989 Size: 367 5292 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2897 2980 4435 618 Size: 325 217 627 1110 83 1455 1842 Fragments arranged by size: 1842 1455 1110 627 325 217 83 BanII G_rGCy'C Cuts at: 1391 2514 2978 1391 Size: 1123 464 4072 Fragments arranged by size: 4072 1123 464 BbeI G_GCGC'C Cuts at: 2901 2901 Size: 5659 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2092 928 Size: 971 193 4495 Fragments arranged by size: 4495 971 193 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3850 3884 1903 Size: 34 1913 34 3678 Fragments arranged by size: 3678 1913 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3551 4761 3551 Size: 1210 4449 BclI T'GATC_A Cuts at: 1548 1548 Size: 5659 BfrBI ATG'CAT Cuts at: 2292 2292 Size: 5659 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4270 136 Size: 107 122 71 631 605 2598 1525 Fragments arranged by size: 2598 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5654 5654 Size: 5659 BlpI GC'TnA_GC Cuts at: 3076 3076 Size: 5659 Bme1580I G_kGCm'C Cuts at: 1790 2426 2514 3720 4876 1790 Size: 636 88 1206 1156 2573 Fragments arranged by size: 2573 1206 1156 636 88 BmgBI CAC'GTC Cuts at: 1318 2442 1318 Size: 1124 4535 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3390 4348 442 Size: 416 53 83 399 24 1973 958 1753 Fragments arranged by size: 1973 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5659 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2901 2901 Size: 5659 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2434 2900 4319 1239 Size: 1195 466 1419 2579 Fragments arranged by size: 2579 1419 1195 466 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2540 3784 4649 4947 2540 Size: 1244 865 298 3252 Fragments arranged by size: 3252 1244 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2653 4322 882 Size: 617 1154 1669 2219 Fragments arranged by size: 2219 1669 1154 617 BsaAI yAC'GTr Cuts at: 493 1204 3450 493 Size: 711 2246 2702 Fragments arranged by size: 2702 2246 711 BsaBI GATnn'nnATC Cuts at: 5653 5653 Size: 5659 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2898 3466 3848 4483 Size: 53 83 186 936 1365 568 382 635 Cuts at: 4483 275 Size: 1451 Fragments arranged by size: 1451 1365 936 635 568 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4092 4490 4764 4911 5322 4092 Size: 398 274 147 411 4429 Fragments arranged by size: 4429 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5434 Fragments arranged by size: 5434 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2069 2716 2819 1186 Size: 883 647 103 4026 Fragments arranged by size: 4026 883 647 103 BseYI C'CCAG_C Cuts at: 2044 2119 4862 5358 2044 Size: 75 2743 496 2345 Fragments arranged by size: 2743 2345 496 75 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5086 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3109 3870 4898 1540 Size: 275 186 1108 761 1028 2301 Fragments arranged by size: 2301 1108 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2426 2914 3720 3805 4876 2426 Size: 488 806 85 1071 3209 Fragments arranged by size: 3209 1071 806 488 85 BsmI GAATG_Cn' Cuts at: 2887 3170 3263 2887 Size: 283 93 5283 Fragments arranged by size: 5283 283 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2426 2514 2914 2978 3720 3805 4876 Size: 399 636 88 400 64 742 85 1071 Cuts at: 4876 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 636 400 399 88 85 64 BspEI T'CCGG_A Cuts at: 5322 5322 Size: 5659 BspHI T'CATG_A Cuts at: 2406 3548 5278 2406 Size: 1142 1730 2787 Fragments arranged by size: 2787 1730 1142 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2729 3014 844 Size: 1885 285 3489 Fragments arranged by size: 3489 1885 285 BsrBI CCG'CTC Cuts at: 1394 1823 3546 1394 Size: 429 1723 3507 Fragments arranged by size: 3507 1723 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4154 4328 65 Size: 1183 2906 174 1396 Fragments arranged by size: 2906 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4303 4490 1700 Size: 110 2493 187 2869 Fragments arranged by size: 2869 2493 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5659 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5659 BssSI C'ACGA_G Cuts at: 3719 4731 3719 Size: 1012 4647 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5659 BstBI TT'CG_AA Cuts at: 4463 4463 Size: 5659 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5659 BstXI CCAn_nnnn'nTGG Cuts at: 2245 2245 Size: 5659 BstYI r'GATC_y Cuts at: 1972 2682 2989 3356 3741 3758 5210 5296 5308 Size: 710 307 367 385 17 1452 86 12 Cuts at: 5308 5654 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 710 385 367 346 307 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2876 2876 Size: 5659 BtgI C'CryG_G Cuts at: 513 1065 2004 2766 513 Size: 552 939 762 3406 Fragments arranged by size: 3406 939 762 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4920 Fragments arranged by size: 4920 683 56 BtsI GCAGTG_nn' Cuts at: 3175 3969 3989 3175 Size: 794 20 4845 Fragments arranged by size: 4845 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5659 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5624 DraI TTT'AAA Cuts at: 2963 2963 Size: 5659 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4666 809 Size: 507 3350 1802 Fragments arranged by size: 3350 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2199 2373 3106 Size: 54 1425 50 275 18 369 174 733 Cuts at: 3106 3995 5624 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 733 369 275 174 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3106 1537 Size: 275 1294 4090 Fragments arranged by size: 4090 1294 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2096 2537 2918 3589 1353 Size: 743 441 381 671 3423 Fragments arranged by size: 3423 743 671 441 381 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4246 4620 4766 387 Size: 1064 138 2657 374 146 1280 Fragments arranged by size: 2657 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2434 2699 2900 3722 4319 5106 5374 Size: 393 802 265 201 822 597 787 268 Cuts at: 5374 1239 Size: 1524 Fragments arranged by size: 1524 822 802 787 597 393 268 265 201 EcoNI CCTnn'n_nnAGG Cuts at: 2362 2362 Size: 5659 EcoO109I rG'GnC_Cy Cuts at: 2511 2511 Size: 5659 EcoRI G'AATT_C Cuts at: 2968 3346 2968 Size: 378 5281 FspI TGC'GCA Cuts at: 4165 4165 Size: 5659 HaeII r_GCGC'y Cuts at: 1678 2901 4806 1678 Size: 1223 1905 2531 Fragments arranged by size: 2531 1905 1223 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4300 4332 4374 4406 1373 Size: 32 135 32 2728 32 42 32 2626 Fragments arranged by size: 2728 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2584 3003 3249 669 Size: 988 281 646 419 246 3079 Fragments arranged by size: 3079 988 646 419 281 246 HindIII A'AGCT_T Cuts at: 748 2162 748 Size: 1414 4245 HpaI GTT'AAC Cuts at: 2584 3249 2584 Size: 665 4994 KasI G'GCGC_C Cuts at: 2897 2897 Size: 5659 KpnI G_GTAC'C Cuts at: 2984 2984 Size: 5659 MfeI C'AATT_G Cuts at: 3258 3258 Size: 5659 MluI A'CGCG_T Cuts at: 5535 5535 Size: 5659 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2250 2725 4773 4957 1305 Size: 945 475 2048 184 2007 Fragments arranged by size: 2048 2007 945 475 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2201 2375 10 Size: 54 1425 343 369 174 3294 Fragments arranged by size: 3294 1425 369 343 174 54 MslI CAynn'nnrTG Cuts at: 518 1166 2174 2210 2736 2765 3617 3976 4135 Size: 648 1008 36 526 29 852 359 159 Cuts at: 4135 518 Size: 2042 Fragments arranged by size: 2042 1008 852 648 526 359 159 36 29 MspA1I CmG'CkG Cuts at: 1606 3075 3752 4900 5145 1606 Size: 1469 677 1148 245 2120 Fragments arranged by size: 2120 1469 1148 677 245 NarI GG'CG_CC Cuts at: 2898 2898 Size: 5659 NcoI C'CATG_G Cuts at: 513 1065 2004 2766 513 Size: 552 939 762 3406 Fragments arranged by size: 3406 939 762 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5659 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5659 NotI GC'GGCC_GC Cuts at: 3106 3106 Size: 5659 NsiI A_TGCA'T Cuts at: 2294 2294 Size: 5659 NspI r_CATG'y Cuts at: 1132 1229 2215 2659 3017 4562 1132 Size: 97 986 444 358 1545 2229 Fragments arranged by size: 2229 1545 986 444 358 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5659 PciI A'CATG_T Cuts at: 2211 4558 2211 Size: 2347 3312 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5659 PmeI GTTT'AAAC Cuts at: 2963 2963 Size: 5659 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3728 3760 5241 5273 3728 Size: 32 1481 32 4114 Fragments arranged by size: 4114 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5659 PsiI TTA'TAA Cuts at: 3229 3229 Size: 5659 PspOMI G'GGCC_C Cuts at: 2510 2510 Size: 5659 PstI C_TGCA'G Cuts at: 830 3011 830 Size: 2181 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5659 SalI G'TCGA_C Cuts at: 1936 3001 1936 Size: 1065 4594 SbfI CC_TGCA'GG Cuts at: 3011 3011 Size: 5659 ScaI AGT'ACT Cuts at: 3907 5619 3907 Size: 1712 3947 SfcI C'TryA_G Cuts at: 826 1045 2869 3007 4142 4823 5014 826 Size: 219 1824 138 1135 681 191 1471 Fragments arranged by size: 1824 1471 1135 681 219 191 138 SfoI GGC'GCC Cuts at: 2899 2899 Size: 5659 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5659 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5659 SmaI CCC'GGG Cuts at: 1872 2516 2986 1872 Size: 644 470 4545 Fragments arranged by size: 4545 644 470 SmlI C'TyrA_G Cuts at: 820 1017 1943 2555 3763 4664 4926 820 Size: 197 926 612 1208 901 262 1553 Fragments arranged by size: 1553 1208 926 901 612 262 197 SnaBI TAC'GTA Cuts at: 493 3450 493 Size: 2957 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5659 SphI G_CATG'C Cuts at: 1132 3017 1132 Size: 1885 3774 SspI AAT'ATT Cuts at: 5 52 3583 5 Size: 47 3531 2081 Fragments arranged by size: 3531 2081 47 StuI AGG'CCT Cuts at: 1517 2711 1517 Size: 1194 4465 StyI C'CwwG_G Cuts at: 513 1065 2004 2766 3098 513 Size: 552 939 762 332 3074 Fragments arranged by size: 3074 939 762 552 332 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2188 3686 3703 3856 4041 4486 325 Size: 1255 608 1498 17 153 185 445 1498 Fragments arranged by size: 1498 1498 1255 608 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2178 2394 3905 Size: 274 80 33 51 1443 201 216 1511 Cuts at: 3905 5617 96 Size: 1712 138 Fragments arranged by size: 1712 1511 1443 274 216 201 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5619 5651 5619 Size: 32 5627 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5659 XbaI T'CTAG_A Cuts at: 2995 2995 Size: 5659 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5659 XmaI C'CCGG_G Cuts at: 1870 2514 2984 1870 Size: 644 470 4545 Fragments arranged by size: 4545 644 470 XmnI GAAnn'nnTTC Cuts at: 3788 3788 Size: 5659 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3467 276 Size: 53 83 186 936 1933 2468 Fragments arranged by size: 2468 1933 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI