################################## # NO CUT ENZYMES ( 156 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AgeI AhdI AlwNI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BanI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BspDI BspEI BspHI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HapII HgaI Hin1I HincII HindII HindIII HpaI HpaII KasI KpnI Ksp632I KspI MaeII MamI MfeI MluI MroI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NotI NruI NsiI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StyI SwaI Tth111I Van91I XcmI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AflIII 206 ACATGT A^C[GA][CT]GT Alw44I 417 GTGCAC G^TGCAC AocI 870 CCTTAGG CC^T[ACGT]AGG ApaLI 417 GTGCAC G^TGCAC AspHI 417 GTGCAC G[AT]GC[AT]^C AvaI 219 CTCGAG C^[CT]CG[GA]G BamHI 677 GGATCC G^GATCC BbsI 178 GTCTTC GAAGAC(2/6) BpuAI 178 GTCTTC GAAGAC(2/6) BsaI 642 GGTCTC GGTCTC(1/5) BsaJI 132 CCTGGG C^C[ACGT][ACGT]GG BseRI 720 CTCCTC GAGGAG(10/8) BsgI 160 CTGCAC GTGCAG(16/14) BsiHKAI 417 GTGCAC G[AT]GC[AT]^C BsmAI 643 GTCTC GTCTC(1/5) BsmFI 229 GTCCC GGGAC(10/14) BsmI 876 GAATGC GAATGC(1/-1) BsoBI 219 CTCGAG C^[CT]CG[GA]G Bsp1407I 770 TGTACA T^GTACA BspLU11I 206 ACATGT A^CATGT BspMI 715 ACCTGC ACCTGC(4/8) BsrBI 625 CCGCTC CCGCTC(-3/-3) BsrGI 770 TGTACA T^GTACA Bsu36I 870 CCTTAGG CC^T[ACGT]AGG Cac8I 240 GCTAGC GC[ACGT]^[ACGT]GC CfoI 758 GCGC GCG^C Eco81I 870 CCTTAGG CC^T[ACGT]AGG FauI 598 GCGGG CCCGC(4/6) HaeII 757 AGCGCC [GA]GCGC^[CT] HhaI 758 GCGC GCG^C HinP1I 758 GCGC G^CGC MseI 106 TTAA T^TAA MwoI 598 GCGGGTTCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NheI 240 GCTAGC G^CTAGC NspI 206 ACATGT [GA]CATG^[CT] PaeR7I 219 CTCGAG C^TCGAG ScaI 223 AGTACT AGT^ACT SexAI 581 ACCAGGT A^CC[AT]GGT SspBI 770 TGTACA T^GTACA StuI 704 AGGCCT AGG^CCT TaqI 220 TCGA T^CGA TfiI 44 GATTC G^A[AT]TC Tru9I 106 TTAA T^TAA Tsp509I 554 AATT ^AATT TspRI 420 CACTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 220 TCGA T^CGA XbaI 154 TCTAGA T^CTAGA XhoI 219 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AfaI 224 GTAC GT^AC 771 GTAC BalI 194 TGGCCA TGG^CCA 665 TGGCCA BanII 136 GGGCTC G[GA]GC[CT]^C 893 GGGCTC BbvI 755 GCAGC GCAGC(8/12) 492 GCTGC BpmI 64 CTGGAG CTGGAG(16/14) 408 CTGGAG BsrI 407 ACTGG ACTGG(1/-1) 681 CCAGT BstF5I 33 CATCC GGATG(2/0) 657 CATCC BstYI 677 GGATCC [GA]^GATC[CT] 812 AGATCC EaeI 194 TGGCCA [CT]^GGCC[GA] 665 TGGCCA FokI 33 CATCC GGATG(9/13) 657 CATCC MaeIII 186 GTCAC ^GT[ACGT]AC 377 GTCAC MboII 810 GAAGA GAAGA(8/7) 179 TCTTC MflI 677 GGATCC [GA]^GATC[CT] 812 AGATCC MluNI 194 TGGCCA TGG^CCA 665 TGGCCA MscI 194 TGGCCA TGG^CCA 665 TGGCCA PleI 151 GACTC GAGTC(4/5) 905 GAGTC RsaI 224 GTAC GT^AC 771 GTAC SfaNI 237 GATGC GCATC(5/9) 255 GATGC SfcI 99 CTACAG C^T[GA][CT]AG 864 CTACAG TseI 492 GCTGC G^C[AT]GC 755 GCAGC Tsp45I 186 GTCAC ^GT[GC]AC 377 GTCAC XhoII 677 GGATCC [GA]^GATC[CT] 812 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 32 enzymes) # ######################################### AciI AluI AlwI AvaII BfaI BmyI BsiYI BslI Bsp1286I BssKI BstNI Cfr13I DdeI DpnII EcoRII Fnu4HI HaeIII HinfI HphI ItaI MaeI MboI MnlI MslI MvaI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SmlI