(Circular) (Six-base) MAPSORT of: pFN21AE5936.seq Check: 3723 from: 1 to: 5638 pFN21AE5936 5638 bp With 182 enzymes: SgfI * December 8, 2014 13:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3448 278 Size: 53 83 186 936 1912 2468 Fragments arranged by size: 2468 1912 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2598 2981 1937 Size: 661 383 4594 Fragments arranged by size: 4594 661 383 Acc65I G'GTAC_C Cuts at: 2959 2959 Size: 5638 AclI AA'CG_TT Cuts at: 1563 3765 4138 1563 Size: 2202 373 3063 Fragments arranged by size: 3063 2202 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2921 3701 5085 5353 1632 Size: 1289 780 1384 268 1917 Fragments arranged by size: 1917 1384 1289 780 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5441 AflIII A'CryG_T Cuts at: 1203 2577 4537 5514 1203 Size: 1374 1960 977 1327 Fragments arranged by size: 1960 1374 1327 977 AgeI A'CCGG_T Cuts at: 2367 4469 2367 Size: 2102 3536 AhdI GACnn_n'nnGTC Cuts at: 4367 4367 Size: 5638 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5638 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5638 AlwNI CAG_nnn'CTG Cuts at: 2123 2508 3381 4953 2123 Size: 385 873 1572 2808 Fragments arranged by size: 2808 1572 873 385 ApaLI G'TGCA_C Cuts at: 2329 3695 4851 2329 Size: 1366 1156 3116 Fragments arranged by size: 3116 1366 1156 ApoI r'AATT_y Cuts at: 1493 2947 3173 3325 5434 1493 Size: 1454 226 152 2109 1697 Fragments arranged by size: 2109 1697 1454 226 152 AseI AT'TA_AT Cuts at: 160 1032 4192 160 Size: 872 3160 1606 Fragments arranged by size: 3160 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5638 AvaI C'yCGr_G Cuts at: 1870 1943 2784 2963 1870 Size: 73 841 179 4545 Fragments arranged by size: 4545 841 179 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2291 2324 2291 Size: 33 5605 BamHI G'GATC_C Cuts at: 2968 3335 2968 Size: 367 5271 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2959 4414 618 Size: 325 217 627 1172 1455 1842 Fragments arranged by size: 1842 1455 1172 627 325 217 BanII G_rGCy'C Cuts at: 1391 2424 2957 1391 Size: 1033 533 4072 Fragments arranged by size: 4072 1033 533 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4667 BbvCI CC'TCA_GC Cuts at: 2501 2501 Size: 5638 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3829 3863 1903 Size: 34 1892 34 3678 Fragments arranged by size: 3678 1892 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3530 4740 3530 Size: 1210 4428 BclI T'GATC_A Cuts at: 1548 2133 1548 Size: 585 5053 BfrBI ATG'CAT Cuts at: 2664 2685 2664 Size: 21 5617 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2357 4249 136 Size: 107 122 71 631 605 685 1892 1525 Fragments arranged by size: 1892 1525 685 631 605 122 107 71 BglII A'GATC_T Cuts at: 2481 2930 5633 2481 Size: 449 2703 2486 Fragments arranged by size: 2703 2486 449 BlpI GC'TnA_GC Cuts at: 3055 3055 Size: 5638 Bme1580I G_kGCm'C Cuts at: 1790 2241 2333 3699 4855 1790 Size: 451 92 1366 1156 2573 Fragments arranged by size: 2573 1366 1156 451 92 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5638 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3369 4327 442 Size: 416 53 83 399 24 1952 958 1753 Fragments arranged by size: 1952 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5638 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2535 4298 1239 Size: 1296 1763 2579 Fragments arranged by size: 2579 1763 1296 Bpu10I CC'TnA_GC Cuts at: 2501 2501 Size: 5638 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2086 3763 4628 4926 2086 Size: 1677 865 298 2798 Fragments arranged by size: 2798 1677 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2207 4301 882 Size: 617 708 2094 2219 Fragments arranged by size: 2219 2094 708 617 BsaAI yAC'GTr Cuts at: 493 1204 3429 493 Size: 711 2225 2702 Fragments arranged by size: 2702 2225 711 BsaBI GATnn'nnATC Cuts at: 2138 5632 2138 Size: 3494 2144 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3445 3827 4462 275 Size: 53 83 186 936 1912 382 635 1451 Fragments arranged by size: 1912 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2367 4071 4469 4743 4890 5301 2367 Size: 1704 398 274 147 411 2704 Fragments arranged by size: 2704 1704 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2053 2083 2204 2234 2477 Size: 30 165 30 650 30 121 30 243 Cuts at: 2477 2507 1178 Size: 30 4309 Fragments arranged by size: 4309 650 243 165 121 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5638 BseYI C'CCAG_C Cuts at: 4841 5337 4841 Size: 496 5142 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2153 2554 1163 Size: 573 417 401 4247 Fragments arranged by size: 4247 573 417 401 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3088 3849 4877 1540 Size: 275 186 1087 761 1028 2301 Fragments arranged by size: 2301 1087 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2333 3699 3784 4855 2333 Size: 1366 85 1071 3116 Fragments arranged by size: 3116 1366 1071 85 BsmI GAATG_Cn' Cuts at: 2664 3149 3242 2664 Size: 485 93 5060 Fragments arranged by size: 5060 485 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2241 2333 2424 2957 3699 3784 4855 Size: 399 451 92 91 533 742 85 1071 Cuts at: 4855 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 533 451 399 92 91 85 BspEI T'CCGG_A Cuts at: 5301 5301 Size: 5638 BspHI T'CATG_A Cuts at: 3527 5257 3527 Size: 1730 3908 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2993 844 Size: 2149 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3525 1394 Size: 429 1702 3507 Fragments arranged by size: 3507 1702 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2316 4133 4307 65 Size: 1183 1068 1817 174 1396 Fragments arranged by size: 1817 1396 1183 1068 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2367 4282 4469 1700 Size: 110 557 1915 187 2869 Fragments arranged by size: 2869 1915 557 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5638 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5638 BssSI C'ACGA_G Cuts at: 2025 3698 4710 2025 Size: 1673 1012 2953 Fragments arranged by size: 2953 1673 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5638 BstBI TT'CG_AA Cuts at: 4442 4442 Size: 5638 BstEII G'GTnAC_C Cuts at: 1184 2740 1184 Size: 1556 4082 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 5638 BstYI r'GATC_y Cuts at: 1972 2481 2930 2968 3335 3720 3737 5189 5275 Size: 509 449 38 367 385 17 1452 86 Cuts at: 5275 5287 5633 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 509 449 385 367 346 86 38 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2355 2416 513 Size: 552 939 351 61 3735 Fragments arranged by size: 3735 939 552 351 61 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4899 Fragments arranged by size: 4899 683 56 BtsI GCAGTG_nn' Cuts at: 2165 3154 3948 3968 2165 Size: 989 794 20 3835 Fragments arranged by size: 3835 989 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5638 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5603 DraI TTT'AAA Cuts at: 2942 2942 Size: 5638 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4645 809 Size: 507 3329 1802 Fragments arranged by size: 3329 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2162 2193 2349 Size: 54 1425 50 275 18 332 31 156 Cuts at: 2349 2376 2670 3085 3974 5603 8 Size: 27 294 415 889 1629 43 Fragments arranged by size: 1629 1425 889 415 332 294 275 156 54 50 43 31 27 18 EagI C'GGCC_G Cuts at: 1537 1812 3085 1537 Size: 275 1273 4090 Fragments arranged by size: 4090 1273 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3568 1353 Size: 2215 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2420 4225 4599 4745 387 Size: 1064 138 831 1805 374 146 1280 Fragments arranged by size: 1805 1280 1064 831 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2535 2921 3701 4298 5085 5353 1239 Size: 393 903 386 780 597 787 268 1524 Fragments arranged by size: 1524 903 787 780 597 393 386 268 EcoRI G'AATT_C Cuts at: 2947 3325 2947 Size: 378 5260 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2503 2503 Size: 5638 FspI TGC'GCA Cuts at: 4144 4144 Size: 5638 HaeII r_GCGC'y Cuts at: 1678 4785 1678 Size: 3107 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4279 4311 4353 4385 1373 Size: 32 135 32 2707 32 42 32 2626 Fragments arranged by size: 2707 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2982 3228 669 Size: 988 281 1044 246 3079 Fragments arranged by size: 3079 1044 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5638 HpaI GTT'AAC Cuts at: 3228 3228 Size: 5638 KpnI G_GTAC'C Cuts at: 2963 2963 Size: 5638 MfeI C'AATT_G Cuts at: 3237 3237 Size: 5638 MluI A'CGCG_T Cuts at: 5514 5514 Size: 5638 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 1992 4752 4936 1305 Size: 687 2760 184 2007 Fragments arranged by size: 2760 2007 687 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2195 2378 2672 10 Size: 54 1425 343 363 183 294 2976 Fragments arranged by size: 2976 1425 363 343 294 183 54 MslI CAynn'nnrTG Cuts at: 518 1166 2045 2228 2576 3596 3955 4114 518 Size: 648 879 183 348 1020 359 159 2042 Fragments arranged by size: 2042 1020 879 648 359 348 183 159 MspA1I CmG'CkG Cuts at: 1606 3054 3731 4879 5124 1606 Size: 1448 677 1148 245 2120 Fragments arranged by size: 2120 1448 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2355 2416 513 Size: 552 939 351 61 3735 Fragments arranged by size: 3735 939 552 351 61 NdeI CA'TA_TG Cuts at: 387 387 Size: 5638 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5638 NotI GC'GGCC_GC Cuts at: 3085 3085 Size: 5638 NsiI A_TGCA'T Cuts at: 2666 2687 2666 Size: 21 5617 NspI r_CATG'y Cuts at: 1132 1229 2581 2689 2996 4541 1132 Size: 97 1352 108 307 1545 2229 Fragments arranged by size: 2229 1545 1352 307 108 97 PasI CC'CwG_GG Cuts at: 1348 2057 1348 Size: 709 4929 PciI A'CATG_T Cuts at: 2577 4537 2577 Size: 1960 3678 PflMI CCAn_nnn'nTGG Cuts at: 1108 2734 1108 Size: 1626 4012 PmeI GTTT'AAAC Cuts at: 2942 2942 Size: 5638 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3707 3739 5220 5252 3707 Size: 32 1481 32 4093 Fragments arranged by size: 4093 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2027 1613 Size: 414 5224 PsiI TTA'TAA Cuts at: 3208 3208 Size: 5638 PspXI vC'TCGA_Gb Cuts at: 2784 2784 Size: 5638 PstI C_TGCA'G Cuts at: 830 2990 830 Size: 2160 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5638 SalI G'TCGA_C Cuts at: 1936 2980 1936 Size: 1044 4594 SbfI CC_TGCA'GG Cuts at: 2990 2990 Size: 5638 ScaI AGT'ACT Cuts at: 2556 3886 5598 2556 Size: 1330 1712 2596 Fragments arranged by size: 2596 1712 1330 SexAI A'CCwGG_T Cuts at: 2216 2216 Size: 5638 SfcI C'TryA_G Cuts at: 826 1045 2986 4121 4802 4993 826 Size: 219 1941 1135 681 191 1471 Fragments arranged by size: 1941 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2357 2357 Size: 5638 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5638 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5638 SmaI CCC'GGG Cuts at: 1872 2965 1872 Size: 1093 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2065 2784 3742 4643 4905 820 Size: 197 926 122 719 958 901 262 1553 Fragments arranged by size: 1553 958 926 901 719 262 197 122 SnaBI TAC'GTA Cuts at: 493 3429 493 Size: 2936 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5638 SphI G_CATG'C Cuts at: 1132 2689 2996 1132 Size: 1557 307 3774 Fragments arranged by size: 3774 1557 307 SspI AAT'ATT Cuts at: 5 52 3562 5 Size: 47 3510 2081 Fragments arranged by size: 3510 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5638 StyI C'CwwG_G Cuts at: 513 1065 2004 2355 2416 3077 513 Size: 552 939 351 61 661 3074 Fragments arranged by size: 3074 939 661 552 351 61 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3665 3682 3835 4020 4465 325 Size: 1255 2085 17 153 185 445 1498 Fragments arranged by size: 2085 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2181 2307 2554 Size: 274 80 33 51 1443 204 126 247 Cuts at: 2554 3884 5596 96 Size: 1330 1712 138 Fragments arranged by size: 1712 1443 1330 274 247 204 138 126 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5598 5630 5598 Size: 32 5606 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5638 XbaI T'CTAG_A Cuts at: 2974 2974 Size: 5638 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2041 2590 2041 Size: 549 5089 XhoI C'TCGA_G Cuts at: 1943 2784 1943 Size: 841 4797 XmaI C'CCGG_G Cuts at: 1870 2963 1870 Size: 1093 4545 XmnI GAAnn'nnTTC Cuts at: 2708 3767 2708 Size: 1059 4579 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3446 276 Size: 53 83 186 936 1912 2468 Fragments arranged by size: 2468 1912 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI SalI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PsrI PvuII RsrII SacI SacII SanDI SapI SfoI SrfI SwaI