################################## # NO CUT ENZYMES ( 153 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII AgeI AhdI AocI Aor51HI ApaI AscI Asp718I AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BcnI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI Eam1105I EarI Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HpaI HpaII KasI KpnI Ksp632I KspI MaeI MaeII MaeIII MamI MfeI MflI MluI MroI MslI MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RcaI RsrII SacII SalI SapI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI SspBI SspI StuI SwaI TfiI Tsp45I Tth111I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccI 814 GTCTAC GT^[AC][GT]AC AfaI 225 GTAC GT^AC Alw44I 190 GTGCAC G^TGCAC AlwI 138 GATCC GGATC(4/5) AlwNI 886 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 190 GTGCAC G^TGCAC AseI 610 ATTAAT AT^TAAT AsnI 610 ATTAAT AT^TAAT AvaII 666 GGACC G^G[AT]CC BalI 796 TGGCCA TGG^CCA BbsI 50 GTCTTC GAAGAC(2/6) BclI 749 TGATCA T^GATCA BpuAI 50 GTCTTC GAAGAC(2/6) BseRI 621 GAGGAG GAGGAG(10/8) BsiEI 240 CGGCCG CG[GA][CT]^CG BsmI 638 GAATGC GAATGC(1/-1) BspMI 116 GCAGGT ACCTGC(4/8) BstAPI 654 GCACAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 869 TTCGAA TT^CGAA Cfr13I 666 GGACC G^G[ACGT]CC DraII 665 AGGACCC [GA]G^G[ACGT]CC[CT] DsaI 799 CCATGG C^C[GA][CT]GG EagI 240 CGGCCG C^GGCCG EclXI 240 CGGCCG C^GGCCG Eco52I 240 CGGCCG C^GGCCG EcoO109I 665 AGGACCC [GA]G^G[ACGT]CC[CT] FauI 161 CCCGC CCCGC(4/6) FbaI 749 TGATCA T^GATCA HindIII 865 AAGCTT A^AGCTT HinfI 48 GAGTC G^A[ACGT]TC HphI 450 GGTGA GGTGA(8/7) MluNI 796 TGGCCA TGG^CCA MscI 796 TGGCCA TGG^CCA MspA1I 300 CAGCTG C[AC]G^C[GT]G NcoI 799 CCATGG C^CATGG NspV 869 TTCGAA TT^CGAA PacI 607 TTAATTAA TTAAT^TAA PflMI 284 CCACCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 48 GAGTC GAGTC(4/5) PpuMI 665 AGGACCC [GA]G^G[AT]CC[CT] PshBI 610 ATTAAT AT^TAAT PvuII 300 CAGCTG CAG^CTG RsaI 225 GTAC GT^AC SacI 692 GAGCTC GAGCT^C Sau96I 666 GGACC G^G[ACGT]CC SbfI 660 CCTGCAGG CCTGCA^GG ScaI 224 AGTACT AGT^ACT SfaNI 757 GATGC GCATC(5/9) SfuI 869 TTCGAA TT^CGAA SmlI 862 CTCAAG C^T[CT][GA]AG Sse8387I 660 CCTGCAGG CCTGCA^GG Van91I 284 CCACCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AciI 162 CCGC CCGC(-3/-1) 761 CCGC Asp700I 69 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 485 GAAAATTTTC AspHI 190 GTGCAC G[AT]GC[AT]^C 692 GAGCTC BanI 154 GGTGCC G^G[CT][GA]CC 387 GGTGCC BanII 405 GGGCTC G[GA]GC[CT]^C 692 GAGCTC BsaI 235 GGTCTC GGTCTC(1/5) 591 GGTCTC BsiHKAI 190 GTGCAC G[AT]GC[AT]^C 692 GAGCTC BsmAI 236 GTCTC GTCTC(1/5) 592 GTCTC EaeI 240 CGGCCG [CT]^GGCC[GA] 796 TGGCCA EcoT14I 128 CCTTGG C^C[AT][AT]GG 799 CCATGG PstI 172 CTGCAG CTGCA^G 661 CTGCAG SfcI 172 CTGCAG C^T[GA][CT]AG 661 CTGCAG StyI 128 CCTTGG C^C[AT][AT]GG 799 CCATGG TaqI 221 TCGA T^CGA 870 TCGA TthHB8I 221 TCGA T^CGA 870 TCGA XmnI 69 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 485 GAAAATTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 37 enzymes) # ######################################### AcsI AluI ApoI BbvI BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I DdeI DpnII EcoRII Fnu4HI FokI HaeIII ItaI MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII NlaIV Sau3AI ScrFI Tru9I TseI Tsp509I TspRI