(Circular) (Six-base) MAPSORT of: pFN21AE5358.seq Check: 5343 from: 1 to: 5626 pFN21AE5358 5626 bp With 182 enzymes: SgfI * January 30, 2014 17:18 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3436 278 Size: 53 83 186 936 1900 2468 Fragments arranged by size: 2468 1900 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2820 2969 1937 Size: 883 149 4594 Fragments arranged by size: 4594 883 149 Acc65I G'GTAC_C Cuts at: 2947 2947 Size: 5626 AclI AA'CG_TT Cuts at: 1563 3753 4126 1563 Size: 2190 373 3063 Fragments arranged by size: 3063 2190 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2390 2723 2842 3689 5073 5341 1632 Size: 758 333 119 847 1384 268 1917 Fragments arranged by size: 1917 1384 847 758 333 268 119 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5429 AflIII A'CryG_T Cuts at: 1203 4525 5502 1203 Size: 3322 977 1327 Fragments arranged by size: 3322 1327 977 AgeI A'CCGG_T Cuts at: 4457 4457 Size: 5626 AhdI GACnn_n'nnGTC Cuts at: 4355 4355 Size: 5626 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5626 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2144 1184 Size: 960 4666 AlwNI CAG_nnn'CTG Cuts at: 2896 3369 4941 2896 Size: 473 1572 3581 Fragments arranged by size: 3581 1572 473 ApaLI G'TGCA_C Cuts at: 2195 3683 4839 2195 Size: 1488 1156 2982 Fragments arranged by size: 2982 1488 1156 ApoI r'AATT_y Cuts at: 1493 2477 2492 2771 2935 3161 3313 5422 1493 Size: 984 15 279 164 226 152 2109 1697 Fragments arranged by size: 2109 1697 984 279 226 164 152 15 AseI AT'TA_AT Cuts at: 160 1032 2616 4180 160 Size: 872 1584 1564 1606 Fragments arranged by size: 1606 1584 1564 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5626 AvaI C'yCGr_G Cuts at: 1870 1943 2951 1870 Size: 73 1008 4545 Fragments arranged by size: 4545 1008 73 BamHI G'GATC_C Cuts at: 2956 3323 2956 Size: 367 5259 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2159 2392 2947 4402 618 Size: 325 217 627 372 233 555 1455 1842 Fragments arranged by size: 1842 1455 627 555 372 325 233 217 BanII G_rGCy'C Cuts at: 1391 2414 2701 2945 1391 Size: 1023 287 244 4072 Fragments arranged by size: 4072 1023 287 244 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2048 928 Size: 971 149 4506 Fragments arranged by size: 4506 971 149 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3817 3851 1903 Size: 34 50 34 1796 34 3678 Fragments arranged by size: 3678 1796 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3518 4728 3518 Size: 1210 4416 BclI T'GATC_A Cuts at: 1548 2754 1548 Size: 1206 4420 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4237 136 Size: 107 122 71 631 605 2565 1525 Fragments arranged by size: 2565 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5621 5621 Size: 5626 BlpI GC'TnA_GC Cuts at: 3043 3043 Size: 5626 Bme1580I G_kGCm'C Cuts at: 1790 2199 2397 2661 3687 4843 1790 Size: 409 198 264 1026 1156 2573 Fragments arranged by size: 2573 1156 1026 409 264 198 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5626 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3357 4315 442 Size: 416 53 83 399 24 1940 958 1753 Fragments arranged by size: 1940 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5626 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2652 2652 Size: 5626 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4286 1239 Size: 3047 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2852 3751 4616 4914 2852 Size: 899 865 298 3564 Fragments arranged by size: 3564 899 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2246 2602 4289 882 Size: 617 747 356 1687 2219 Fragments arranged by size: 2219 1687 747 617 356 BsaAI yAC'GTr Cuts at: 493 1204 3417 493 Size: 711 2213 2702 Fragments arranged by size: 2702 2213 711 BsaBI GATnn'nnATC Cuts at: 5620 5620 Size: 5626 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3433 3815 4450 275 Size: 53 83 186 936 1900 382 635 1451 Fragments arranged by size: 1900 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4059 4457 4731 4878 5289 4059 Size: 398 274 147 411 4396 Fragments arranged by size: 4396 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5401 Fragments arranged by size: 5401 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2641 1186 Size: 1455 4171 BseYI C'CCAG_C Cuts at: 2095 2675 4829 5325 2095 Size: 580 2154 496 2396 Fragments arranged by size: 2396 2154 580 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5053 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2248 3076 3837 4865 1540 Size: 275 186 247 828 761 1028 2301 Fragments arranged by size: 2301 1028 828 761 275 247 186 BsiHKAI G_wGCw'C Cuts at: 2199 2701 3687 3772 4843 2199 Size: 502 986 85 1071 2982 Fragments arranged by size: 2982 1071 986 502 85 BsmI GAATG_Cn' Cuts at: 2649 3137 3230 2649 Size: 488 93 5045 Fragments arranged by size: 5045 488 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2199 2397 2414 2661 2701 2945 3687 Size: 399 409 198 17 247 40 244 742 Cuts at: 3687 3772 4843 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 409 399 247 244 198 85 40 17 BspEI T'CCGG_A Cuts at: 5289 5289 Size: 5626 BspHI T'CATG_A Cuts at: 3515 5245 3515 Size: 1730 3896 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2112 2981 844 Size: 1268 869 3489 Fragments arranged by size: 3489 1268 869 BsrBI CCG'CTC Cuts at: 1394 1823 3513 1394 Size: 429 1690 3507 Fragments arranged by size: 3507 1690 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4121 4295 65 Size: 1183 2873 174 1396 Fragments arranged by size: 2873 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4270 4457 1700 Size: 110 2460 187 2869 Fragments arranged by size: 2869 2460 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5626 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5626 BssSI C'ACGA_G Cuts at: 3686 4698 3686 Size: 1012 4614 BstAPI GCAn_nnn'nTGC Cuts at: 794 2665 794 Size: 1871 3755 BstBI TT'CG_AA Cuts at: 2875 4430 2875 Size: 1555 4071 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5626 BstYI r'GATC_y Cuts at: 1972 2956 3323 3708 3725 5177 5263 5275 5621 Size: 984 367 385 17 1452 86 12 346 Cuts at: 5621 1972 Size: 1977 Fragments arranged by size: 1977 1452 984 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2804 513 Size: 552 1739 3335 Fragments arranged by size: 3335 1739 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4887 Fragments arranged by size: 4887 683 56 BtsI GCAGTG_nn' Cuts at: 3142 3936 3956 3142 Size: 794 20 4812 Fragments arranged by size: 4812 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5626 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5591 DraI TTT'AAA Cuts at: 2930 2930 Size: 5626 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4633 809 Size: 507 3317 1802 Fragments arranged by size: 3317 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2245 2801 2922 Size: 54 1425 50 275 18 415 556 121 Cuts at: 2922 3073 3962 5591 8 Size: 151 889 1629 43 Fragments arranged by size: 1629 1425 889 556 415 275 151 121 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2245 3073 1537 Size: 275 433 828 4090 Fragments arranged by size: 4090 828 433 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3556 1353 Size: 2203 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4213 4587 4733 387 Size: 1064 138 2624 374 146 1280 Fragments arranged by size: 2624 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2699 2699 Size: 5626 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2390 2723 2842 3689 4286 5073 5341 Size: 393 758 333 119 847 597 787 268 Cuts at: 5341 1239 Size: 1524 Fragments arranged by size: 1524 847 787 758 597 393 333 268 119 EcoO109I rG'GnC_Cy Cuts at: 2671 2671 Size: 5626 EcoRI G'AATT_C Cuts at: 2935 3313 2935 Size: 378 5248 FspI TGC'GCA Cuts at: 4132 4132 Size: 5626 HaeII r_GCGC'y Cuts at: 1678 4773 1678 Size: 3095 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4267 4299 4341 4373 1373 Size: 32 135 32 2695 32 42 32 2626 Fragments arranged by size: 2695 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2970 3216 669 Size: 988 281 1032 246 3079 Fragments arranged by size: 3079 1032 988 281 246 HindIII A'AGCT_T Cuts at: 748 2870 748 Size: 2122 3504 HpaI GTT'AAC Cuts at: 3216 3216 Size: 5626 KpnI G_GTAC'C Cuts at: 2951 2951 Size: 5626 MfeI C'AATT_G Cuts at: 3225 3225 Size: 5626 MluI A'CGCG_T Cuts at: 5502 5502 Size: 5626 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4740 4924 1305 Size: 3435 184 2007 Fragments arranged by size: 3435 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2803 10 Size: 54 1425 343 971 2833 Fragments arranged by size: 2833 1425 971 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3584 3943 4102 518 Size: 648 2418 359 159 2042 Fragments arranged by size: 2418 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2307 3042 3719 4867 5112 1606 Size: 701 735 677 1148 245 2120 Fragments arranged by size: 2120 1148 735 701 677 245 NcoI C'CATG_G Cuts at: 513 1065 2804 513 Size: 552 1739 3335 Fragments arranged by size: 3335 1739 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5626 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5626 NotI GC'GGCC_GC Cuts at: 3073 3073 Size: 5626 NspI r_CATG'y Cuts at: 1132 1229 2984 4529 1132 Size: 97 1755 1545 2229 Fragments arranged by size: 2229 1755 1545 97 PacI TTA_AT'TAA Cuts at: 2616 2616 Size: 5626 PasI CC'CwG_GG Cuts at: 1348 2384 1348 Size: 1036 4590 PciI A'CATG_T Cuts at: 4525 4525 Size: 5626 PflMI CCAn_nnn'nTGG Cuts at: 1108 2295 1108 Size: 1187 4439 PmeI GTTT'AAAC Cuts at: 2930 2930 Size: 5626 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3695 3727 5208 5240 3695 Size: 32 1481 32 4081 Fragments arranged by size: 4081 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2671 2671 Size: 5626 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5626 PsiI TTA'TAA Cuts at: 3196 3196 Size: 5626 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2495 2527 2780 2812 2495 Size: 32 253 32 5309 Fragments arranged by size: 5309 253 32 32 PstI C_TGCA'G Cuts at: 830 2181 2670 2978 830 Size: 1351 489 308 3478 Fragments arranged by size: 3478 1351 489 308 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5626 PvuII CAG'CTG Cuts at: 2307 2307 Size: 5626 SacI G_AGCT'C Cuts at: 2701 2701 Size: 5626 SalI G'TCGA_C Cuts at: 1936 2968 1936 Size: 1032 4594 SbfI CC_TGCA'GG Cuts at: 2670 2978 2670 Size: 308 5318 ScaI AGT'ACT Cuts at: 2231 3874 5586 2231 Size: 1643 1712 2271 Fragments arranged by size: 2271 1712 1643 SfcI C'TryA_G Cuts at: 826 1045 2177 2666 2974 4109 4790 4981 826 Size: 219 1132 489 308 1135 681 191 1471 Fragments arranged by size: 1471 1135 1132 681 489 308 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5626 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5626 SmaI CCC'GGG Cuts at: 1872 2953 1872 Size: 1081 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2867 3730 4631 4893 820 Size: 197 926 924 863 901 262 1553 Fragments arranged by size: 1553 926 924 901 863 262 197 SnaBI TAC'GTA Cuts at: 493 3417 493 Size: 2924 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5626 SphI G_CATG'C Cuts at: 1132 2984 1132 Size: 1852 3774 SspI AAT'ATT Cuts at: 5 52 3550 5 Size: 47 3498 2081 Fragments arranged by size: 3498 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5626 StyI C'CwwG_G Cuts at: 513 1065 2133 2804 3065 513 Size: 552 1068 671 261 3074 Fragments arranged by size: 3074 1068 671 552 261 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2147 2380 2443 2696 2908 3653 3670 Size: 1255 567 233 63 253 212 745 17 Cuts at: 3670 3823 4008 4453 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 745 567 445 253 233 212 185 153 63 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2229 3872 5584 Size: 274 80 33 51 1443 252 1643 1712 Cuts at: 5584 96 Size: 138 Fragments arranged by size: 1712 1643 1443 274 252 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5586 5618 5586 Size: 32 5594 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5626 XbaI T'CTAG_A Cuts at: 2962 2962 Size: 5626 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5626 XmaI C'CCGG_G Cuts at: 1870 2951 1870 Size: 1081 4545 XmnI GAAnn'nnTTC Cuts at: 2078 2494 3755 2078 Size: 416 1261 3949 Fragments arranged by size: 3949 1261 416 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3434 276 Size: 53 83 186 936 1900 2468 Fragments arranged by size: 2468 1900 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PacI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PfoI PmlI PspOMI PspXI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI