(Circular) (Six-base) MAPSORT of: pFN21AE6397.seq Check: 3963 from: 1 to: 5374 pFN21AE6397 5374 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3184 278 Size: 53 83 186 936 1648 2468 Fragments arranged by size: 2468 1648 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2538 2717 1937 Size: 601 179 4594 Fragments arranged by size: 4594 601 179 Acc65I G'GTAC_C Cuts at: 2695 2695 Size: 5374 AclI AA'CG_TT Cuts at: 1563 3501 3874 1563 Size: 1938 373 3063 Fragments arranged by size: 3063 1938 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2224 2237 2271 3437 4821 5089 1632 Size: 592 13 34 1166 1384 268 1917 Fragments arranged by size: 1917 1384 1166 592 268 34 13 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5177 AflIII A'CryG_T Cuts at: 1203 4273 5250 1203 Size: 3070 977 1327 Fragments arranged by size: 3070 1327 977 AgeI A'CCGG_T Cuts at: 4205 4205 Size: 5374 AhdI GACnn_n'nnGTC Cuts at: 4103 4103 Size: 5374 AleI CACnn'nnGTG Cuts at: 1166 2406 2585 1166 Size: 1240 179 3955 Fragments arranged by size: 3955 1240 179 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2081 1184 Size: 897 4477 AlwNI CAG_nnn'CTG Cuts at: 2419 2549 2636 3117 4689 2419 Size: 130 87 481 1572 3104 Fragments arranged by size: 3104 1572 481 130 87 ApaI G_GGCC'C Cuts at: 2047 2047 Size: 5374 ApaLI G'TGCA_C Cuts at: 3431 4587 3431 Size: 1156 4218 ApoI r'AATT_y Cuts at: 1493 2683 2909 3061 5170 1493 Size: 1190 226 152 2109 1697 Fragments arranged by size: 2109 1697 1190 226 152 AseI AT'TA_AT Cuts at: 160 1032 3928 160 Size: 872 2896 1606 Fragments arranged by size: 2896 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5374 AvaI C'yCGr_G Cuts at: 1870 1943 2444 2699 1870 Size: 73 501 255 4545 Fragments arranged by size: 4545 501 255 73 BamHI G'GATC_C Cuts at: 2704 3071 2704 Size: 367 5007 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2099 2329 2555 2695 4150 Size: 325 217 627 312 230 226 140 1455 Cuts at: 4150 618 Size: 1842 Fragments arranged by size: 1842 1455 627 325 312 230 226 217 140 BanII G_rGCy'C Cuts at: 1391 2047 2693 1391 Size: 656 646 4072 Fragments arranged by size: 4072 656 646 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2277 928 Size: 971 378 4025 Fragments arranged by size: 4025 971 378 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3565 3599 1903 Size: 34 50 34 1544 34 3678 Fragments arranged by size: 3678 1544 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3266 4476 3266 Size: 1210 4164 BclI T'GATC_A Cuts at: 1548 1548 Size: 5374 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3985 136 Size: 107 122 71 631 605 2313 1525 Fragments arranged by size: 2313 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5369 5369 Size: 5374 BlpI GC'TnA_GC Cuts at: 2791 2791 Size: 5374 Bme1580I G_kGCm'C Cuts at: 1790 2047 2070 2451 2558 2627 3435 4591 1790 Size: 257 23 381 107 69 808 1156 2573 Fragments arranged by size: 2573 1156 808 381 257 107 69 23 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5374 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3105 4063 442 Size: 416 53 83 399 24 1688 958 1753 Fragments arranged by size: 1753 1688 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5374 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2515 2641 4034 1239 Size: 1276 126 1393 2579 Fragments arranged by size: 2579 1393 1276 126 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3499 4364 4662 3499 Size: 865 298 4211 Fragments arranged by size: 4211 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4037 882 Size: 617 2538 2219 Fragments arranged by size: 2538 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3165 493 Size: 711 1961 2702 Fragments arranged by size: 2702 1961 711 BsaBI GATnn'nnATC Cuts at: 5368 5368 Size: 5374 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3181 3563 4198 275 Size: 53 83 186 936 1648 382 635 1451 Fragments arranged by size: 1648 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3807 4205 4479 4626 5037 3807 Size: 398 274 147 411 4144 Fragments arranged by size: 4144 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2449 2479 2510 2540 1178 Size: 30 165 30 1046 30 31 30 4012 Fragments arranged by size: 4012 1046 165 31 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2266 2311 2518 1186 Size: 1080 45 207 4042 Fragments arranged by size: 4042 1080 207 45 BseYI C'CCAG_C Cuts at: 2008 2095 2542 4577 5073 2008 Size: 87 447 2035 496 2309 Fragments arranged by size: 2309 2035 496 447 87 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2422 2519 1163 Size: 573 686 97 4018 Fragments arranged by size: 4018 686 573 97 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2824 3585 4613 1540 Size: 275 186 823 761 1028 2301 Fragments arranged by size: 2301 1028 823 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3435 3520 4591 3435 Size: 85 1071 4218 Fragments arranged by size: 4218 1071 85 BsmI GAATG_Cn' Cuts at: 2885 2978 2885 Size: 93 5281 BsmBI CGTCTCn'nnnn_ Cuts at: 2281 2281 Size: 5374 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2047 2070 2451 2558 2627 2693 3435 Size: 399 257 23 381 107 69 66 742 Cuts at: 3435 3520 4591 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 381 257 107 85 69 66 23 BspEI T'CCGG_A Cuts at: 5037 5037 Size: 5374 BspHI T'CATG_A Cuts at: 3263 4993 3263 Size: 1730 3644 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2729 844 Size: 1885 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3261 1394 Size: 429 1438 3507 Fragments arranged by size: 3507 1438 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3869 4043 65 Size: 1183 2621 174 1396 Fragments arranged by size: 2621 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4018 4205 1700 Size: 110 2208 187 2869 Fragments arranged by size: 2869 2208 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5374 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5374 BssSI C'ACGA_G Cuts at: 2104 3434 4446 2104 Size: 1330 1012 3032 Fragments arranged by size: 3032 1330 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5374 BstBI TT'CG_AA Cuts at: 4178 4178 Size: 5374 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5374 BstXI CCAn_nnnn'nTGG Cuts at: 2161 2161 Size: 5374 BstYI r'GATC_y Cuts at: 1972 2704 3071 3456 3473 4925 5011 5023 5369 Size: 732 367 385 17 1452 86 12 346 Cuts at: 5369 1972 Size: 1977 Fragments arranged by size: 1977 1452 732 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2084 513 Size: 552 1019 3803 Fragments arranged by size: 3803 1019 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4635 Fragments arranged by size: 4635 683 56 BtsI GCAGTG_nn' Cuts at: 2890 3684 3704 2890 Size: 794 20 4560 Fragments arranged by size: 4560 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5374 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5339 DraI TTT'AAA Cuts at: 2678 2678 Size: 5374 DraIII CAC_nnn'GTG Cuts at: 2386 2386 Size: 5374 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4381 809 Size: 507 3065 1802 Fragments arranged by size: 3065 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2821 3710 5339 Size: 54 1425 50 275 18 991 889 1629 Cuts at: 5339 8 Size: 43 Fragments arranged by size: 1629 1425 991 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2821 1537 Size: 275 1009 4090 Fragments arranged by size: 4090 1009 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3304 1353 Size: 1951 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3961 4335 4481 387 Size: 1064 138 2372 374 146 1280 Fragments arranged by size: 2372 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2224 2237 2271 2515 2641 3437 4034 Size: 393 592 13 34 244 126 796 597 Cuts at: 4034 4821 5089 1239 Size: 787 268 1524 Fragments arranged by size: 1524 796 787 597 592 393 268 244 126 34 13 EcoO109I rG'GnC_Cy Cuts at: 2044 2616 2662 2044 Size: 572 46 4756 Fragments arranged by size: 4756 572 46 EcoRI G'AATT_C Cuts at: 2683 3061 2683 Size: 378 4996 EcoRV GAT'ATC Cuts at: 2259 2259 Size: 5374 FspI TGC'GCA Cuts at: 3880 3880 Size: 5374 HaeII r_GCGC'y Cuts at: 1678 2216 4521 1678 Size: 538 2305 2531 Fragments arranged by size: 2531 2305 538 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4015 4047 4089 4121 1373 Size: 32 135 32 2443 32 42 32 2626 Fragments arranged by size: 2626 2443 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2718 2964 669 Size: 988 281 780 246 3079 Fragments arranged by size: 3079 988 780 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5374 HpaI GTT'AAC Cuts at: 2964 2964 Size: 5374 KpnI G_GTAC'C Cuts at: 2699 2699 Size: 5374 MfeI C'AATT_G Cuts at: 2973 2973 Size: 5374 MluI A'CGCG_T Cuts at: 5250 5250 Size: 5374 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4488 4672 1305 Size: 3183 184 2007 Fragments arranged by size: 3183 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3552 Fragments arranged by size: 3552 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2063 2406 2585 3332 3691 3850 518 Size: 648 897 343 179 747 359 159 2042 Fragments arranged by size: 2042 897 747 648 359 343 179 159 MspA1I CmG'CkG Cuts at: 1606 2790 3467 4615 4860 1606 Size: 1184 677 1148 245 2120 Fragments arranged by size: 2120 1184 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4822 NdeI CA'TA_TG Cuts at: 387 387 Size: 5374 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5374 NotI GC'GGCC_GC Cuts at: 2821 2821 Size: 5374 NspI r_CATG'y Cuts at: 1132 1229 2455 2732 4277 1132 Size: 97 1226 277 1545 2229 Fragments arranged by size: 2229 1545 1226 277 97 PasI CC'CwG_GG Cuts at: 1348 2563 1348 Size: 1215 4159 PciI A'CATG_T Cuts at: 4273 4273 Size: 5374 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5374 PmeI GTTT'AAAC Cuts at: 2678 2678 Size: 5374 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3443 3475 4956 4988 3443 Size: 32 1481 32 3829 Fragments arranged by size: 3829 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2662 2662 Size: 5374 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5374 PsiI TTA'TAA Cuts at: 2944 2944 Size: 5374 PspOMI G'GGCC_C Cuts at: 2043 2043 Size: 5374 PstI C_TGCA'G Cuts at: 830 2641 2726 830 Size: 1811 85 3478 Fragments arranged by size: 3478 1811 85 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5374 SalI G'TCGA_C Cuts at: 1936 2716 1936 Size: 780 4594 SanDI GG'GwC_CC Cuts at: 2662 2662 Size: 5374 SbfI CC_TGCA'GG Cuts at: 2726 2726 Size: 5374 ScaI AGT'ACT Cuts at: 3622 5334 3622 Size: 1712 3662 SfcI C'TryA_G Cuts at: 826 1045 2637 2722 3857 4538 4729 826 Size: 219 1592 85 1135 681 191 1471 Fragments arranged by size: 1592 1471 1135 681 219 191 85 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5374 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5374 SmaI CCC'GGG Cuts at: 1872 2446 2701 1872 Size: 574 255 4545 Fragments arranged by size: 4545 574 255 SmlI C'TyrA_G Cuts at: 820 1017 1943 3478 4379 4641 820 Size: 197 926 1535 901 262 1553 Fragments arranged by size: 1553 1535 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3165 493 Size: 2672 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5374 SphI G_CATG'C Cuts at: 1132 2732 1132 Size: 1600 3774 SspI AAT'ATT Cuts at: 5 52 3298 5 Size: 47 3246 2081 Fragments arranged by size: 3246 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5374 StyI C'CwwG_G Cuts at: 513 1065 2619 2646 2813 513 Size: 552 1554 27 167 3074 Fragments arranged by size: 3074 1554 552 167 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2087 3401 3418 3571 3756 4201 325 Size: 1255 507 1314 17 153 185 445 1498 Fragments arranged by size: 1498 1314 1255 507 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3620 5332 96 Size: 274 80 33 51 1443 1643 1712 138 Fragments arranged by size: 1712 1643 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2372 2404 2449 2481 5334 5366 2372 Size: 32 45 32 2853 32 2380 Fragments arranged by size: 2853 2380 45 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5374 XbaI T'CTAG_A Cuts at: 2710 2710 Size: 5374 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5374 XmaI C'CCGG_G Cuts at: 1870 2444 2699 1870 Size: 574 255 4545 Fragments arranged by size: 4545 574 255 XmnI GAAnn'nnTTC Cuts at: 3503 3503 Size: 5374 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3182 276 Size: 53 83 186 936 1648 2468 Fragments arranged by size: 2468 1648 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI