################################## # NO CUT ENZYMES ( 158 enzymes) # ################################## AatII AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI AspEI AspI AvaII AviII AvrII BalI BamHI BbeI BbrPI BcgI BciVI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRII EcoRV EcoT14I EcoT22I FauI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HpaI KasI KspI MamI MfeI MluI MluNI MroI MscI MunI MvaI MvnI MwoI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tth111I TthHB8I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### Acc65I 498 GGTACC G^GTACC AccI 383 GTAGAC GT^[AC][GT]AC AciI 439 GCGG CCGC(-3/-1) AcsI 798 AAATTC [GA]^AATT[CT] AflIII 721 ACATGT A^C[GA][CT]GT AlwNI 241 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 261 GGGCCC GGGCC^C ApoI 798 AAATTC [GA]^AATT[CT] Asp700I 73 GAAGTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 498 GGTACC G^GTACC AspHI 609 GAGCTC G[AT]GC[AT]^C AvaI 668 CCCGGG C^[CT]CG[GA]G BbsI 28 GAAGAC GAAGAC(2/6) BbvCI 64 CCTCAGC CCTCAGC(-5/-2) BbvI 901 GCAGC GCAGC(8/12) BclI 807 TGATCA T^GATCA BfaI 520 CTAG C^TAG BpmI 781 CTCCAG CTGGAG(16/14) BpuAI 28 GAAGAC GAAGAC(2/6) BsaAI 151 TACGTG [CT]AC^GT[GA] BsiHKAI 609 GAGCTC G[AT]GC[AT]^C BsmAI 909 GAGAC GTCTC(1/5) BsoBI 668 CCCGGG C^[CT]CG[GA]G BspHI 605 TCATGA T^CATGA BspLU11I 721 ACATGT A^CATGT BstF5I 141 CATCC GGATG(2/0) BstYI 210 GGATCT [GA]^GATC[CT] Cac8I 683 GCATGC GC[ACGT]^[ACGT]GC DraII 260 GGGGCCC [GA]G^G[ACGT]CC[CT] EarI 906 GAAGAG CTCTTC(1/4) EcoO109I 260 GGGGCCC [GA]G^G[ACGT]CC[CT] FbaI 807 TGATCA T^GATCA FokI 141 CATCC GGATG(9/13) HincII 571 GTTGAC GT[CT]^[GA]AC HindII 571 GTTGAC GT[CT]^[GA]AC KpnI 498 GGTACC GGTAC^C Ksp632I 906 GAAGAG CTCTTC(1/4) MaeI 520 CTAG C^TAG MaeII 152 ACGT A^CGT MaeIII 844 GTCAC ^GT[ACGT]AC MflI 210 GGATCT [GA]^GATC[CT] MspA1I 67 CAGCTG C[AC]G^C[GT]G NdeI 359 CATATG CA^TATG PvuII 67 CAGCTG CAG^CTG RcaI 605 TCATGA T^CATGA SacI 609 GAGCTC GAGCT^C SmaI 668 CCCGGG CCC^GGG SphI 683 GCATGC GCATG^C TseI 901 GCAGC G^C[AT]GC Tsp45I 844 GTCAC ^GT[GC]AC XhoII 210 GGATCT [GA]^GATC[CT] XmaCI 668 CCCGGG C^CCGGG XmaI 668 CCCGGG C^CCGGG XmnI 73 GAAGTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AlwI 210 GGATC GGATC(4/5) 525 GGATC BanI 498 GGTACC G^G[CT][GA]CC 658 GGTGCC BsaJI 258 CCGGGG C^C[ACGT][ACGT]GG 668 CCCGGG BsrDI 663 CATTGC GCAATG(2/0) 822 CATTGC Cfr13I 261 GGGCC G^G[ACGT]CC 262 GGCCC Fnu4HI 439 GCGGC GC^[ACGT]GC 901 GCAGC HaeIII 62 GGCC GG^CC 262 GGCC HphI 249 GGTGA GGTGA(8/7) 351 GGTGA ItaI 439 GCGGC GC^[ACGT]GC 901 GCAGC MseI 8 TTAA T^TAA 857 TTAA PflMI 426 CCACTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 732 CCATCTTATGG PleI 157 GACTC GAGTC(4/5) 852 GAGTC Sau96I 261 GGGCC G^G[ACGT]CC 262 GGCCC TfiI 252 GAATC G^A[AT]TC 326 GAATC Tru9I 8 TTAA T^TAA 857 TTAA Van91I 426 CCACTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 732 CCATCTTATGG ######################################### # 3 OR MORE CUT ENZYMES ( 30 enzymes) # ######################################### AfaI AluI BanII BcnI BmyI BsiYI BslI Bsp1286I BsrI BssKI DdeI DpnII HapII HinfI HpaII MboI MboII MnlI MslI MspI NciI NdeII NlaIII NlaIV NspI RsaI Sau3AI ScrFI Tsp509I TspRI