################################## # NO CUT ENZYMES ( 163 enzymes) # ################################## AatII AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp700I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HpaI KasI KspI MaeII MamI MfeI MluI MluNI MroI MscI MunI MvnI MwoI NaeI NarI NcoI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### Acc65I 504 GGTACC G^GTACC AccI 389 GTAGAC GT^[AC][GT]AC AciI 522 CCGC CCGC(-3/-1) AcsI 804 AAATTC [GA]^AATT[CT] AflIII 727 ACATGT A^C[GA][CT]GT AlwI 216 GGATC GGATC(4/5) AlwNI 247 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 267 GGGCCC GGGCC^C ApoI 804 AAATTC [GA]^AATT[CT] Asp718I 504 GGTACC G^GTACC AspHI 734 GAGCTC G[AT]GC[AT]^C BbvI 379 GCAGC GCAGC(8/12) BclI 608 TGATCA T^GATCA BcnI 270 CCCGG CC^[GC]GG BpmI 787 CTCCAG CTGGAG(16/14) BsgI 172 CTGCAC GTGCAG(16/14) BsiHKAI 734 GAGCTC G[AT]GC[AT]^C BsmAI 915 GAGAC GTCTC(1/5) BspHI 605 TCATGA T^CATGA BspLU11I 727 ACATGT A^CATGT BspMI 434 ACCTGC ACCTGC(4/8) BsrDI 828 CATTGC GCAATG(2/0) BstNI 159 CCTGG CC^[AT]GG BstYI 216 GGATCT [GA]^GATC[CT] DraII 266 GGGGCCC [GA]G^G[ACGT]CC[CT] DraIII 336 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 770 CGGCCA [CT]^GGCC[GA] EarI 912 GAAGAG CTCTTC(1/4) EcoO109I 266 GGGGCCC [GA]G^G[ACGT]CC[CT] EcoRII 159 CCTGG ^CC[AT]GG FbaI 608 TGATCA T^GATCA Fnu4HI 379 GCAGC GC^[ACGT]GC HincII 696 GTCAAC GT[CT]^[GA]AC HindII 696 GTCAAC GT[CT]^[GA]AC ItaI 379 GCAGC GC^[ACGT]GC KpnI 504 GGTACC GGTAC^C Ksp632I 912 GAAGAG CTCTTC(1/4) MaeIII 850 GTCAC ^GT[ACGT]AC MflI 216 GGATCT [GA]^GATC[CT] MseI 863 TTAA T^TAA MspA1I 380 CAGCTG C[AC]G^C[GT]G MvaI 159 CCTGG CC^[AT]GG NciI 270 CCCGG CC^[GC]GG NheI 25 GCTAGC G^CTAGC NspI 727 ACATGT [GA]CATG^[CT] PflMI 432 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 163 GACTC GAGTC(4/5) PvuII 380 CAGCTG CAG^CTG RcaI 605 TCATGA T^CATGA SacI 734 GAGCTC GAGCT^C SfaNI 690 GATGC GCATC(5/9) SmlI 809 CTCAAG C^T[CT][GA]AG TfiI 303 GATTC G^A[AT]TC Tru9I 863 TTAA T^TAA TseI 379 GCAGC G^C[AT]GC Tsp45I 850 GTCAC ^GT[GC]AC Van91I 432 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 216 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### BanI 504 GGTACC G^G[CT][GA]CC 664 GGTGCC BanII 267 GGGCCC G[GA]GC[CT]^C 734 GAGCTC BfaI 26 CTAG C^TAG 857 CTAG BmyI 267 GGGCCC G[AGT]GC[ACT]^C 734 GAGCTC Bsp1286I 267 GGGCCC G[AGT]GC[ACT]^C 734 GAGCTC BssKI 159 CCTGG ^CC[ACGT]GG 270 CCCGG Cac8I 25 GCTAGC GC[ACGT]^[ACGT]GC 438 GCTGGC Cfr13I 267 GGGCC G^G[ACGT]CC 268 GGCCC HapII 271 CCGG C^CGG 769 CCGG HinfI 163 GACTC G^A[ACGT]TC 303 GATTC HpaII 271 CCGG C^CGG 769 CCGG HphI 357 GGTGA GGTGA(8/7) 494 TCACC MaeI 26 CTAG C^TAG 857 CTAG MslI 177 CACACCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 586 CATGTCAATG MspI 271 CCGG C^CGG 769 CCGG Sau96I 267 GGGCC G^G[ACGT]CC 268 GGCCC ScrFI 159 CCTGG CC^[ACGT]GG 270 CCCGG Tsp509I 64 AATT ^AATT 805 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 19 enzymes) # ######################################### AfaI AluI BsiYI BslI BsrI BstF5I DdeI DpnII FokI HaeIII MboI MboII MnlI NdeII NlaIII NlaIV RsaI Sau3AI TspRI