(Circular) (Six-base) MAPSORT of: pFN21AE5933.seq Check: 2278 from: 1 to: 5638 pFN21AE5933 5638 bp With 182 enzymes: SgfI * December 8, 2014 13:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2448 2448 Size: 5638 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3448 278 Size: 53 83 186 936 1912 2468 Fragments arranged by size: 2468 1912 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2395 2981 1937 Size: 458 586 4594 Fragments arranged by size: 4594 586 458 Acc65I G'GTAC_C Cuts at: 2509 2959 2509 Size: 450 5188 AclI AA'CG_TT Cuts at: 1563 3765 4138 1563 Size: 2202 373 3063 Fragments arranged by size: 3063 2202 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2699 3701 5085 5353 1632 Size: 1067 1002 1384 268 1917 Fragments arranged by size: 1917 1384 1067 1002 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5441 AflIII A'CryG_T Cuts at: 1203 2732 4537 5514 1203 Size: 1529 1805 977 1327 Fragments arranged by size: 1805 1529 1327 977 AgeI A'CCGG_T Cuts at: 4469 4469 Size: 5638 AhdI GACnn_n'nnGTC Cuts at: 4367 4367 Size: 5638 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5638 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5638 AlwNI CAG_nnn'CTG Cuts at: 2257 3381 4953 2257 Size: 1124 1572 2942 Fragments arranged by size: 2942 1572 1124 ApaI G_GGCC'C Cuts at: 2276 2276 Size: 5638 ApaLI G'TGCA_C Cuts at: 3695 4851 3695 Size: 1156 4482 ApoI r'AATT_y Cuts at: 1493 2809 2947 3173 3325 5434 1493 Size: 1316 138 226 152 2109 1697 Fragments arranged by size: 2109 1697 1316 226 152 138 AseI AT'TA_AT Cuts at: 160 1032 4192 160 Size: 872 3160 1606 Fragments arranged by size: 3160 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5638 AvaI C'yCGr_G Cuts at: 1870 1943 2963 1870 Size: 73 1020 4545 Fragments arranged by size: 4545 1020 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2501 2534 2577 2610 2501 Size: 33 43 33 5529 Fragments arranged by size: 5529 43 33 33 BamHI G'GATC_C Cuts at: 2968 3335 2968 Size: 367 5271 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2509 2669 2959 4414 618 Size: 325 217 627 722 160 290 1455 1842 Fragments arranged by size: 1842 1455 722 627 325 290 217 160 BanII G_rGCy'C Cuts at: 1391 2276 2743 2957 1391 Size: 885 467 214 4072 Fragments arranged by size: 4072 885 467 214 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4667 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3829 3863 1903 Size: 34 1892 34 3678 Fragments arranged by size: 3678 1892 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3530 4740 3530 Size: 1210 4428 BclI T'GATC_A Cuts at: 1548 2613 1548 Size: 1065 4573 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4249 136 Size: 107 122 71 631 605 2577 1525 Fragments arranged by size: 2577 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5633 5633 Size: 5638 BlpI GC'TnA_GC Cuts at: 3055 3055 Size: 5638 Bme1580I G_kGCm'C Cuts at: 1790 2276 3699 4855 1790 Size: 486 1423 1156 2573 Fragments arranged by size: 2573 1423 1156 486 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5638 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2342 2718 3369 Size: 416 53 83 399 24 925 376 651 Cuts at: 3369 4327 442 Size: 958 1753 Fragments arranged by size: 1753 958 925 651 416 399 376 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2034 1056 Size: 978 4660 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2777 4298 1239 Size: 1538 1521 2579 Fragments arranged by size: 2579 1538 1521 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2799 3763 4628 4926 2799 Size: 964 865 298 3511 Fragments arranged by size: 3511 964 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4301 882 Size: 617 2802 2219 Fragments arranged by size: 2802 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3429 493 Size: 711 2225 2702 Fragments arranged by size: 2702 2225 711 BsaBI GATnn'nnATC Cuts at: 5632 5632 Size: 5638 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3445 3827 4462 275 Size: 53 83 186 936 1912 382 635 1451 Fragments arranged by size: 1912 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4071 4469 4743 4890 5301 4071 Size: 398 274 147 411 4408 Fragments arranged by size: 4408 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2153 2183 1178 Size: 30 165 30 750 30 4633 Fragments arranged by size: 4633 750 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5638 BseYI C'CCAG_C Cuts at: 4841 5337 4841 Size: 496 5142 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2162 1163 Size: 573 426 4639 Fragments arranged by size: 4639 573 426 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3088 3849 4877 1540 Size: 275 186 1087 761 1028 2301 Fragments arranged by size: 2301 1087 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2743 3699 3784 4855 2743 Size: 956 85 1071 3526 Fragments arranged by size: 3526 1071 956 85 BsmI GAATG_Cn' Cuts at: 3149 3242 3149 Size: 93 5545 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2276 2743 2957 3699 3784 4855 1391 Size: 399 486 467 214 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 486 467 399 214 85 BspEI T'CCGG_A Cuts at: 5301 5301 Size: 5638 BspHI T'CATG_A Cuts at: 2610 3527 5257 2610 Size: 917 1730 2991 Fragments arranged by size: 2991 1730 917 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2448 2993 844 Size: 1604 545 3489 Fragments arranged by size: 3489 1604 545 BsrBI CCG'CTC Cuts at: 1394 1823 3525 1394 Size: 429 1702 3507 Fragments arranged by size: 3507 1702 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2832 4133 4307 65 Size: 1183 1584 1301 174 1396 Fragments arranged by size: 1584 1396 1301 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4282 4469 1700 Size: 110 2472 187 2869 Fragments arranged by size: 2869 2472 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5638 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5638 BssSI C'ACGA_G Cuts at: 3698 4710 3698 Size: 1012 4626 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5638 BstBI TT'CG_AA Cuts at: 4442 4442 Size: 5638 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5638 BstYI r'GATC_y Cuts at: 1972 2221 2968 3335 3720 3737 5189 5275 5287 Size: 249 747 367 385 17 1452 86 12 Cuts at: 5287 5633 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 747 385 367 346 249 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5086 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4899 Fragments arranged by size: 4899 683 56 BtsI GCAGTG_nn' Cuts at: 2919 3154 3948 3968 2919 Size: 235 794 20 4589 Fragments arranged by size: 4589 794 235 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5638 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5603 DraI TTT'AAA Cuts at: 2942 2942 Size: 5638 DraIII CAC_nnn'GTG Cuts at: 2346 2346 Size: 5638 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4645 809 Size: 507 3329 1802 Fragments arranged by size: 3329 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2775 3085 3974 Size: 54 1425 50 275 18 945 310 889 Cuts at: 3974 5603 8 Size: 1629 43 Fragments arranged by size: 1629 1425 945 889 310 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3085 1537 Size: 275 1273 4090 Fragments arranged by size: 4090 1273 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2912 3568 1353 Size: 1559 656 3423 Fragments arranged by size: 3423 1559 656 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4225 4599 4745 387 Size: 1064 138 2636 374 146 1280 Fragments arranged by size: 2636 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2741 2741 Size: 5638 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2699 2777 3701 4298 5085 5353 1239 Size: 393 1067 78 924 597 787 268 1524 Fragments arranged by size: 1524 1067 924 787 597 393 268 78 EcoO109I rG'GnC_Cy Cuts at: 2272 2272 Size: 5638 EcoRI G'AATT_C Cuts at: 2947 3325 2947 Size: 378 5260 FspI TGC'GCA Cuts at: 4144 4144 Size: 5638 HaeII r_GCGC'y Cuts at: 1678 4785 1678 Size: 3107 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4279 4311 4353 4385 1373 Size: 32 135 32 2707 32 42 32 2626 Fragments arranged by size: 2707 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2703 2982 3228 669 Size: 988 281 765 279 246 3079 Fragments arranged by size: 3079 988 765 281 279 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5638 HpaI GTT'AAC Cuts at: 3228 3228 Size: 5638 KpnI G_GTAC'C Cuts at: 2513 2963 2513 Size: 450 5188 MfeI C'AATT_G Cuts at: 3237 3237 Size: 5638 MluI A'CGCG_T Cuts at: 5514 5514 Size: 5638 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2038 4752 4936 1305 Size: 733 2714 184 2007 Fragments arranged by size: 2714 2007 733 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3816 Fragments arranged by size: 3816 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2186 2595 3596 3955 4114 518 Size: 648 1020 409 1001 359 159 2042 Fragments arranged by size: 2042 1020 1001 648 409 359 159 MspA1I CmG'CkG Cuts at: 1606 2387 3054 3731 4879 5124 1606 Size: 781 667 677 1148 245 2120 Fragments arranged by size: 2120 1148 781 677 667 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5086 NdeI CA'TA_TG Cuts at: 387 387 Size: 5638 NheI G'CTAG_C Cuts at: 1052 2030 1052 Size: 978 4660 NotI GC'GGCC_GC Cuts at: 3085 3085 Size: 5638 NspI r_CATG'y Cuts at: 1132 1229 2736 2996 4541 1132 Size: 97 1507 260 1545 2229 Fragments arranged by size: 2229 1545 1507 260 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5638 PciI A'CATG_T Cuts at: 2732 4537 2732 Size: 1805 3833 PflMI CCAn_nnn'nTGG Cuts at: 1108 2443 1108 Size: 1335 4303 PmeI GTTT'AAAC Cuts at: 2942 2942 Size: 5638 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3707 3739 5220 5252 3707 Size: 32 1481 32 4093 Fragments arranged by size: 4093 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5638 PsiI TTA'TAA Cuts at: 3208 3208 Size: 5638 PspOMI G'GGCC_C Cuts at: 2272 2272 Size: 5638 PstI C_TGCA'G Cuts at: 830 2990 830 Size: 2160 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5638 PvuII CAG'CTG Cuts at: 2387 2387 Size: 5638 SacI G_AGCT'C Cuts at: 2743 2743 Size: 5638 SalI G'TCGA_C Cuts at: 1936 2980 1936 Size: 1044 4594 SbfI CC_TGCA'GG Cuts at: 2990 2990 Size: 5638 ScaI AGT'ACT Cuts at: 3886 5598 3886 Size: 1712 3926 SfcI C'TryA_G Cuts at: 826 1045 2986 4121 4802 4993 826 Size: 219 1941 1135 681 191 1471 Fragments arranged by size: 1941 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5638 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5638 SmaI CCC'GGG Cuts at: 1872 2965 1872 Size: 1093 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2814 3742 4643 4905 820 Size: 197 926 871 928 901 262 1553 Fragments arranged by size: 1553 928 926 901 871 262 197 SnaBI TAC'GTA Cuts at: 493 3429 493 Size: 2936 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5638 SphI G_CATG'C Cuts at: 1132 2996 1132 Size: 1864 3774 SspI AAT'ATT Cuts at: 5 52 3562 5 Size: 47 3510 2081 Fragments arranged by size: 3510 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5638 StyI C'CwwG_G Cuts at: 513 1065 3077 513 Size: 552 2012 3074 Fragments arranged by size: 3074 2012 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3665 3682 3835 4020 4465 325 Size: 1255 2085 17 153 185 445 1498 Fragments arranged by size: 2085 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2190 2686 3884 Size: 274 80 33 51 1443 213 496 1198 Cuts at: 3884 5596 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1198 496 274 213 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5598 5630 5598 Size: 32 5606 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5638 XbaI T'CTAG_A Cuts at: 2974 2974 Size: 5638 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5638 XmaI C'CCGG_G Cuts at: 1870 2963 1870 Size: 1093 4545 XmnI GAAnn'nnTTC Cuts at: 3767 3767 Size: 5638 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3446 276 Size: 53 83 186 936 1912 2468 Fragments arranged by size: 2468 1912 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI