################################## # NO CUT ENZYMES ( 201 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaHI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstUI BstXI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRII EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII HphI KasI KpnI KspI MaeI MaeII MaeIII MfeI MluI MluNI MroI MscI MslI MspA1I MspI MunI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScrFI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI TaqI Tsp45I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AcsI 215 GAATTT [GA]^AATT[CT] AlwNI 68 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 193 CCTAAGG CC^T[ACGT]AGG ApoI 215 GAATTT [GA]^AATT[CT] AvaI 413 CTCGGG C^[CT]CG[GA]G BbvI 245 GCAGC GCAGC(8/12) BsaBI 334 GATCCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 278 GGTCTC GGTCTC(1/5) BsiYI 130 CCCTATCAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 130 CCCTATCAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 279 GTCTC GTCTC(1/5) BsoBI 413 CTCGGG C^[CT]CG[GA]G BspMI 67 GCAGGT ACCTGC(4/8) BstF5I 223 CATCC GGATG(2/0) BstYI 374 GGATCT [GA]^GATC[CT] Bsu36I 193 CCTAAGG CC^T[ACGT]AGG Cac8I 99 GCAAGC GC[ACGT]^[ACGT]GC EarI 9 GAAGAG CTCTTC(1/4) Eco81I 193 CCTAAGG CC^T[ACGT]AGG Fnu4HI 245 GCAGC GC^[ACGT]GC FokI 223 CATCC GGATG(9/13) HaeIII 116 GGCC GG^CC ItaI 245 GCAGC GC^[ACGT]GC Ksp632I 9 GAAGAG CTCTTC(1/4) MamI 334 GATCCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboII 9 GAAGA GAAGA(8/7) MflI 374 GGATCT [GA]^GATC[CT] MnlI 49 CCTC CCTC(7/6) MseI 233 TTAA T^TAA PleI 423 GACTC GAGTC(4/5) ScaI 303 AGTACT AGT^ACT SspI 229 AATATT AAT^ATT TfiI 14 GAATC G^A[AT]TC Tru9I 233 TTAA T^TAA TseI 245 GCAGC G^C[AT]GC XhoII 374 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### AfaI 304 GTAC GT^AC 399 GTAC AluI 102 AGCT AG^CT 411 AGCT AvaII 45 GGACC G^G[AT]CC 70 GGTCC BpmI 50 CTCCAG CTGGAG(16/14) 281 CTCCAG BsmFI 44 GGGAC GGGAC(10/14) 128 GTCCC DraII 44 GGGACCC [GA]G^G[ACGT]CC[CT] 69 AGGTCCT EcoO109I 44 GGGACCC [GA]G^G[ACGT]CC[CT] 69 AGGTCCT HinfI 14 GAATC G^A[ACGT]TC 423 GACTC PpuMI 44 GGGACCC [GA]G^G[AT]CC[CT] 69 AGGTCCT RsaI 304 GTAC GT^AC 399 GTAC TspRI 58 CAGTG CA[GC]TG[ACGT][ACGT]^ 274 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 10 enzymes) # ######################################### AlwI Cfr13I DdeI DpnII MboI NdeII NlaIV Sau3AI Sau96I Tsp509I