################################## # NO CUT ENZYMES ( 134 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AcyI AfaI AflII AgeI AhdI AocI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BanI BbeI BcgI BciVI BclI BfaI BfrI BglII BlnI BsaBI BsaHI BsaI BsaWI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BsrBI BsrDI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI Eam1105I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI Hin1I HindIII HinfI HpaI KasI KpnI KspI MaeI MamI MfeI MflI MluI MroI MseI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmeI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacII SbfI ScaI SexAI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tru9I Tsp509I Tth111I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 26 enzymes) # ################################### Alw44I 445 GTGCAC G^TGCAC AlwNI 835 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 79 AGCGCT AGC^GCT ApaI 112 GGGCCC GGGCC^C ApaLI 445 GTGCAC G^TGCAC BbrPI 880 CACGTG CAC^GTG BpmI 1007 CTGGAG CTGGAG(16/14) BsgI 832 GTGCAG GTGCAG(16/14) BsmAI 407 GTCTC GTCTC(1/5) BspLU11I 386 ACATGT A^CATGT DraII 111 GGGGCCC [GA]G^G[ACGT]CC[CT] EarI 283 CTCTTC CTCTTC(1/4) Eco47III 79 AGCGCT AGC^GCT EcoO109I 111 GGGGCCC [GA]G^G[ACGT]CC[CT] Ksp632I 283 CTCTTC CTCTTC(1/4) MslI 204 CATCTTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG PflMI 579 CCAGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 880 CACGTG CAC^GTG PmlI 880 CACGTG CAC^GTG SacI 280 GAGCTC GAGCT^C SalI 373 GTCGAC G^TCGAC SapI 282 GCTCTTC GCTCTTC(1/4) SfiI 309 GGCCTCGTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmlI 1051 CTCAAG C^T[CT][GA]AG Van91I 579 CCAGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 607 CCACACCCGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccI 373 GTCGAC GT^[AC][GT]AC 637 GTCTAC AflIII 386 ACATGT A^C[GA][CT]GT 881 ACGTGT AlwI 213 GATCC GGATC(4/5) 959 GATCC AvaI 25 CCCGGG C^[CT]CG[GA]G 115 CCCGGG AvaII 426 GGACC G^G[AT]CC 1151 GGACC BbvCI 894 CCTCAGC CCTCAGC(-5/-2) 1015 GCTGAGG BglI 310 GCCTCGTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 616 GCCTGGTCGGC BsiEI 317 CGGCCG CG[GA][CT]^CG 1125 CGGCCG BsoBI 25 CCCGGG C^[CT]CG[GA]G 115 CCCGGG BsrFI 470 ACCGGC [GA]^CCGG[CT] 564 ACCGGC BstAPI 64 GCAGCCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 447 GCACCCTCTGC Cfr10I 470 ACCGGC [GA]^CCGG[CT] 564 ACCGGC DpnII 213 GATC ^GATC 959 GATC EagI 317 CGGCCG C^GGCCG 1125 CGGCCG EclXI 317 CGGCCG C^GGCCG 1125 CGGCCG Eco52I 317 CGGCCG C^GGCCG 1125 CGGCCG HincII 373 GTCGAC GT[CT]^[GA]AC 874 GTCAAC HindII 373 GTCGAC GT[CT]^[GA]AC 874 GTCAAC MaeIII 529 GTCAC ^GT[ACGT]AC 853 GTGAC MboI 213 GATC ^GATC 959 GATC NdeII 213 GATC ^GATC 959 GATC NspI 386 ACATGT [GA]CATG^[CT] 1076 GCATGT PstI 62 CTGCAG CTGCA^G 598 CTGCAG PvuII 835 CAGCTG CAG^CTG 909 CAGCTG Sau3AI 213 GATC ^GATC 959 GATC SfcI 62 CTGCAG C^T[GA][CT]AG 598 CTGCAG SmaI 25 CCCGGG CCC^GGG 115 CCCGGG TaqI 13 TCGA T^CGA 374 TCGA Tsp45I 529 GTCAC ^GT[GC]AC 853 GTGAC TthHB8I 13 TCGA T^CGA 374 TCGA XmaCI 25 CCCGGG C^CCGGG 115 CCCGGG XmaI 25 CCCGGG C^CCGGG 115 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 66 enzymes) # ######################################### AccII AciI AluI AspHI BalI BanII BbsI BbvI BcnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaJI BseRI BsiHKAI BsiYI BslI BsmFI Bsp1286I BspMI BsrI BssHII BssKI BstF5I BstNI BstUI Cac8I CelII CfoI Cfr13I DdeI DsaI EaeI EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HpaII HphI ItaI MaeII MboII MluNI MnlI MscI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV Sau96I ScrFI SfaNI TseI TspRI