(Circular) (Six-base) MAPSORT of: pFN21AE9504.seq Check: 4003 from: 1 to: 5869 pFN21AE9504 5869 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3679 278 Size: 53 83 186 936 2143 2468 Fragments arranged by size: 2468 2143 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2379 2643 3212 1937 Size: 442 264 569 4594 Fragments arranged by size: 4594 569 442 264 Acc65I G'GTAC_C Cuts at: 3190 3190 Size: 5869 AclI AA'CG_TT Cuts at: 1563 3996 4369 1563 Size: 2433 373 3063 Fragments arranged by size: 3063 2433 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3932 5316 5584 1632 Size: 2300 1384 268 1917 Fragments arranged by size: 2300 1917 1384 268 AfeI AGC'GCT Cuts at: 2086 2086 Size: 5869 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5672 AflIII A'CryG_T Cuts at: 1203 2391 2886 4768 5745 1203 Size: 1188 495 1882 977 1327 Fragments arranged by size: 1882 1327 1188 977 495 AgeI A'CCGG_T Cuts at: 4700 4700 Size: 5869 AhdI GACnn_n'nnGTC Cuts at: 4598 4598 Size: 5869 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5869 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5869 AlwNI CAG_nnn'CTG Cuts at: 2845 3612 5184 2845 Size: 767 1572 3530 Fragments arranged by size: 3530 1572 767 ApaI G_GGCC'C Cuts at: 2121 2121 Size: 5869 ApaLI G'TGCA_C Cuts at: 2450 3926 5082 2450 Size: 1476 1156 3237 Fragments arranged by size: 3237 1476 1156 ApoI r'AATT_y Cuts at: 1493 3178 3404 3556 5665 1493 Size: 1685 226 152 2109 1697 Fragments arranged by size: 2109 1697 1685 226 152 AseI AT'TA_AT Cuts at: 160 1032 4423 160 Size: 872 3391 1606 Fragments arranged by size: 3391 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5869 AvaI C'yCGr_G Cuts at: 1870 1943 2030 2120 3194 1870 Size: 73 87 90 1074 4545 Fragments arranged by size: 4545 1074 90 87 73 BamHI G'GATC_C Cuts at: 3199 3566 3199 Size: 367 5502 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3190 4645 618 Size: 325 217 627 1403 1455 1842 Fragments arranged by size: 1842 1455 1403 627 325 217 BanII G_rGCy'C Cuts at: 1391 2121 2289 3033 3188 1391 Size: 730 168 744 155 4072 Fragments arranged by size: 4072 744 730 168 155 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2246 2398 2626 928 Size: 971 347 152 228 4171 Fragments arranged by size: 4171 971 347 228 152 BbvCI CC'TCA_GC Cuts at: 2900 3021 2900 Size: 121 5748 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4060 4094 1903 Size: 34 2123 34 3678 Fragments arranged by size: 3678 2123 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3761 4971 3761 Size: 1210 4659 BclI T'GATC_A Cuts at: 1548 1548 Size: 5869 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2321 2627 4480 Size: 107 122 71 631 605 649 306 1853 Cuts at: 4480 136 Size: 1525 Fragments arranged by size: 1853 1525 649 631 605 306 122 107 71 BglII A'GATC_T Cuts at: 5864 5864 Size: 5869 BlpI GC'TnA_GC Cuts at: 2297 2372 2423 3286 2297 Size: 75 51 863 4880 Fragments arranged by size: 4880 863 75 51 Bme1580I G_kGCm'C Cuts at: 1790 2121 2307 2454 3089 3930 5086 1790 Size: 331 186 147 635 841 1156 2573 Fragments arranged by size: 2573 1156 841 635 331 186 147 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5869 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3600 4558 442 Size: 416 53 83 399 24 2183 958 1753 Fragments arranged by size: 2183 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5869 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3033 4529 1239 Size: 1794 1496 2579 Fragments arranged by size: 2579 1794 1496 Bpu10I CC'TnA_GC Cuts at: 2900 3021 2900 Size: 121 5748 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3041 3994 4859 5157 3041 Size: 953 865 298 3753 Fragments arranged by size: 3753 953 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4532 882 Size: 617 3033 2219 Fragments arranged by size: 3033 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2440 2833 2887 3660 493 Size: 711 1236 393 54 773 2702 Fragments arranged by size: 2702 1236 773 711 393 54 BsaBI GATnn'nnATC Cuts at: 5863 5863 Size: 5869 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3676 4058 4693 275 Size: 53 83 186 936 2143 382 635 1451 Fragments arranged by size: 2143 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4302 4700 4974 5121 5532 4302 Size: 398 274 147 411 4639 Fragments arranged by size: 4639 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5644 Fragments arranged by size: 5644 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2117 3038 3041 1186 Size: 931 921 3 4014 Fragments arranged by size: 4014 931 921 3 BseYI C'CCAG_C Cuts at: 2047 2483 2656 5072 5568 2047 Size: 436 173 2416 496 2348 Fragments arranged by size: 2416 2348 496 436 173 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2858 1163 Size: 573 1122 4174 Fragments arranged by size: 4174 1122 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2325 3133 3319 4080 5108 1540 Size: 275 186 324 808 186 761 1028 2301 Fragments arranged by size: 2301 1028 808 761 324 275 186 186 BsiHKAI G_wGCw'C Cuts at: 2289 2373 2424 2454 2814 3930 4015 5086 2289 Size: 84 51 30 360 1116 85 1071 3072 Fragments arranged by size: 3072 1116 1071 360 85 84 51 30 BsmI GAATG_Cn' Cuts at: 3380 3473 3380 Size: 93 5776 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2121 2289 2307 2373 2424 2454 2814 Size: 399 331 168 18 66 51 30 360 Cuts at: 2814 3033 3089 3188 3930 4015 5086 1391 Size: 219 56 99 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 360 331 219 168 99 85 66 56 51 30 18 BspEI T'CCGG_A Cuts at: 5532 5532 Size: 5869 BspHI T'CATG_A Cuts at: 3758 5488 3758 Size: 1730 4139 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2268 2610 2706 3224 844 Size: 1424 342 96 518 3489 Fragments arranged by size: 3489 1424 518 342 96 BsrBI CCG'CTC Cuts at: 1394 1823 3756 1394 Size: 429 1933 3507 Fragments arranged by size: 3507 1933 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4364 4538 65 Size: 1183 3116 174 1396 Fragments arranged by size: 3116 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2475 2569 4513 4700 1700 Size: 110 665 94 1944 187 2869 Fragments arranged by size: 2869 1944 665 187 110 94 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5869 BssHII G'CGCG_C Cuts at: 1925 2461 2719 2734 1925 Size: 536 258 15 5060 Fragments arranged by size: 5060 536 258 15 BssSI C'ACGA_G Cuts at: 3929 4941 3929 Size: 1012 4857 BstAPI GCAn_nnn'nTGC Cuts at: 794 2075 2458 794 Size: 1281 383 4205 Fragments arranged by size: 4205 1281 383 BstBI TT'CG_AA Cuts at: 4673 4673 Size: 5869 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5869 BstYI r'GATC_y Cuts at: 1972 3199 3566 3951 3968 5420 5506 5518 5864 Size: 1227 367 385 17 1452 86 12 346 Cuts at: 5864 1972 Size: 1977 Fragments arranged by size: 1977 1452 1227 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2445 2589 2724 513 Size: 552 1380 144 135 3658 Fragments arranged by size: 3658 1380 552 144 135 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2530 487 Size: 683 56 1304 3826 Fragments arranged by size: 3826 1304 683 56 BtsI GCAGTG_nn' Cuts at: 2612 3048 3385 4179 4199 2612 Size: 436 337 794 20 4282 Fragments arranged by size: 4282 794 436 337 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5869 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5834 DraI TTT'AAA Cuts at: 3173 3173 Size: 5869 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4876 809 Size: 507 3560 1802 Fragments arranged by size: 3560 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2271 2322 2442 Size: 54 1425 50 275 18 441 51 120 Cuts at: 2442 2490 2581 2609 2679 3130 3316 4205 5834 Size: 48 91 28 70 451 186 889 1629 Cuts at: 5834 8 Size: 43 Fragments arranged by size: 1629 1425 889 451 441 275 186 120 91 70 54 51 50 48 43 28 18 EagI C'GGCC_G Cuts at: 1537 1812 2322 3130 3316 1537 Size: 275 510 808 186 4090 Fragments arranged by size: 4090 808 510 275 186 EarI CTCTTCn'nnn_ Cuts at: 1353 2294 3799 1353 Size: 941 1505 3423 Fragments arranged by size: 3423 1505 941 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2108 2114 2948 4456 4830 4976 Size: 1064 138 519 6 834 1508 374 146 Cuts at: 4976 387 Size: 1280 Fragments arranged by size: 1508 1280 1064 834 519 374 146 138 6 EcoICRI GAG'CTC Cuts at: 2287 2287 Size: 5869 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3033 3932 4529 5316 5584 1239 Size: 393 1401 899 597 787 268 1524 Fragments arranged by size: 1524 1401 899 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2117 2117 Size: 5869 EcoRI G'AATT_C Cuts at: 3178 3556 3178 Size: 378 5491 FspI TGC'GCA Cuts at: 4375 4375 Size: 5869 HaeII r_GCGC'y Cuts at: 1678 2013 2088 2761 5016 1678 Size: 335 75 673 2255 2531 Fragments arranged by size: 2531 2255 673 335 75 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4510 4542 4584 4616 1373 Size: 32 135 32 2938 32 42 32 2626 Fragments arranged by size: 2938 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2380 2881 3213 3459 669 Size: 988 281 442 501 332 246 3079 Fragments arranged by size: 3079 988 501 442 332 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5869 HpaI GTT'AAC Cuts at: 3459 3459 Size: 5869 KpnI G_GTAC'C Cuts at: 3194 3194 Size: 5869 MfeI C'AATT_G Cuts at: 3468 3468 Size: 5869 MluI A'CGCG_T Cuts at: 5745 5745 Size: 5869 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2973 4983 5167 1305 Size: 1668 2010 184 2007 Fragments arranged by size: 2010 2007 1668 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2492 2611 2681 10 Size: 54 1425 343 660 119 70 3198 Fragments arranged by size: 3198 1425 660 343 119 70 54 MslI CAynn'nnrTG Cuts at: 518 1166 2213 3827 4186 4345 518 Size: 648 1047 1614 359 159 2042 Fragments arranged by size: 2042 1614 1047 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2842 2916 2974 3285 3962 5110 5355 1606 Size: 1236 74 58 311 677 1148 245 2120 Fragments arranged by size: 2120 1236 1148 677 311 245 74 58 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5317 NdeI CA'TA_TG Cuts at: 387 387 Size: 5869 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5869 NotI GC'GGCC_GC Cuts at: 3316 3316 Size: 5869 NspI r_CATG'y Cuts at: 1132 1229 2395 3085 3227 4772 1132 Size: 97 1166 690 142 1545 2229 Fragments arranged by size: 2229 1545 1166 690 142 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5869 PciI A'CATG_T Cuts at: 2391 4768 2391 Size: 2377 3492 PflMI CCAn_nnn'nTGG Cuts at: 1108 2590 1108 Size: 1482 4387 PfoI T'CCnGG_A Cuts at: 3000 3000 Size: 5869 PmeI GTTT'AAAC Cuts at: 3173 3173 Size: 5869 PmlI CAC'GTG Cuts at: 2887 2887 Size: 5869 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3938 3970 5451 5483 3938 Size: 32 1481 32 4324 Fragments arranged by size: 4324 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5869 PsiI TTA'TAA Cuts at: 3439 3439 Size: 5869 PspOMI G'GGCC_C Cuts at: 2117 2117 Size: 5869 PstI C_TGCA'G Cuts at: 830 2071 2607 3221 830 Size: 1241 536 614 3478 Fragments arranged by size: 3478 1241 614 536 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5869 PvuII CAG'CTG Cuts at: 2842 2916 2842 Size: 74 5795 SacI G_AGCT'C Cuts at: 2289 2289 Size: 5869 SalI G'TCGA_C Cuts at: 1936 2378 3211 1936 Size: 442 833 4594 Fragments arranged by size: 4594 833 442 SapI GCTCTTCn'nnn_ Cuts at: 2294 2294 Size: 5869 SbfI CC_TGCA'GG Cuts at: 3221 3221 Size: 5869 ScaI AGT'ACT Cuts at: 4117 5829 4117 Size: 1712 4157 SfcI C'TryA_G Cuts at: 826 1045 2067 2603 3217 4352 5033 5224 826 Size: 219 1022 536 614 1135 681 191 1471 Fragments arranged by size: 1471 1135 1022 681 614 536 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2321 2321 Size: 5869 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5869 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5869 SmaI CCC'GGG Cuts at: 1872 2032 2122 3196 1872 Size: 160 90 1074 4545 Fragments arranged by size: 4545 1074 160 90 SmlI C'TyrA_G Cuts at: 820 1017 1943 3056 3973 4874 5136 820 Size: 197 926 1113 917 901 262 1553 Fragments arranged by size: 1553 1113 926 917 901 262 197 SnaBI TAC'GTA Cuts at: 493 3660 493 Size: 3167 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5869 SphI G_CATG'C Cuts at: 1132 3227 1132 Size: 2095 3774 SspI AAT'ATT Cuts at: 5 52 3793 5 Size: 47 3741 2081 Fragments arranged by size: 3741 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5869 StyI C'CwwG_G Cuts at: 513 1065 3308 513 Size: 552 2243 3074 Fragments arranged by size: 3074 2243 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3896 3913 4066 4251 4696 325 Size: 1255 2316 17 153 185 445 1498 Fragments arranged by size: 2316 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4115 5827 96 Size: 274 80 33 51 1443 2138 1712 138 Fragments arranged by size: 2138 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5829 5861 5829 Size: 32 5837 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5869 XbaI T'CTAG_A Cuts at: 3205 3205 Size: 5869 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2619 2619 Size: 5869 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5869 XmaI C'CCGG_G Cuts at: 1870 2030 2120 3194 1870 Size: 160 90 1074 4545 Fragments arranged by size: 4545 1074 160 90 XmnI GAAnn'nnTTC Cuts at: 3998 3998 Size: 5869 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3677 276 Size: 53 83 186 936 2143 2468 Fragments arranged by size: 2468 2143 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspXI PsrI RsrII SacII SanDI SexAI SfoI SrfI SwaI