Restriction map for
pFN21AE5479
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# NO CUT ENZYMES ( 128 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
AlwI Aor51HI ApaI AscI AseI
AsnI Asp718I AspEI AspI AvaII
AvrII BamHI BbeI BbrPI BbvCI
BciVI BfrI BlnI BlpI Bpu1102I
BsaBI BsaHI BsaI BsaWI BseAI
BsiWI BsmAI BsmBI BsmFI BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrI BssHII BssSI Bst1107I BstBI
BstUI BstZ17I CelII ClaI CpoI
DraI DraII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoRI EcoRV EcoT22I FseI HaeII
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MunI MvnI NaeI
NarI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI ScaI SexAI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI TaqI Tth111I
TthHB8I XbaI XhoI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AcsI 886 GAATTT [GA]^AATT[CT]
AflIII 218 ACGTGT A^C[GA][CT]GT
Alw44I 324 GTGCAC G^TGCAC
AlwNI 70 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 873 CCTGAGG CC^T[ACGT]AGG
ApaLI 324 GTGCAC G^TGCAC
ApoI 886 GAATTT [GA]^AATT[CT]
Asp700I 522 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 411 CCCGGG C^[CT]CG[GA]G
BanI 28 GGCACC G^G[CT][GA]CC
BcgI 272 GCATCTCTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 470 TGATCA T^GATCA
BglI 346 GCCTCCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 209 AGATCT A^GATCT
BsgI 564 CTGCAC GTGCAG(16/14)
BsiEI 360 CGACCG CG[GA][CT]^CG
BsmI 894 GAATGC GAATGC(1/-1)
BsoBI 411 CCCGGG C^[CT]CG[GA]G
Bsp1407I 830 TGTACA T^GTACA
BsrFI 694 ACCGGC [GA]^CCGG[CT]
BsrGI 830 TGTACA T^GTACA
BstAPI 69 GCAGTACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 741 GGTCACC G^GT[ACGT]ACC
BstPI 741 GGTCACC G^GT[ACGT]ACC
BstXI 236 CCAAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 209 AGATCT [GA]^GATC[CT]
Bsu36I 873 CCTGAGG CC^T[ACGT]AGG
Cfr10I 694 ACCGGC [GA]^CCGG[CT]
DraIII 327 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 873 CCTGAGG CC^T[ACGT]AGG
EcoO65I 741 GGTCACC G^GT[ACGT]ACC
EcoT14I 350 CCATGG C^C[AT][AT]GG
FauI 384 CCCGC CCCGC(4/6)
FbaI 470 TGATCA T^GATCA
HinfI 796 GATTC G^A[ACGT]TC
MflI 209 AGATCT [GA]^GATC[CT]
MseI 890 TTAA T^TAA
NcoI 350 CCATGG C^CATGG
NdeI 650 CATATG CA^TATG
PflMI 426 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 427 CAGCTG CAG^CTG
SapI 78 GCTCTTC GCTCTTC(1/4)
SfiI 345 GGCCTCCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 411 CCCGGG CCC^GGG
SmlI 553 CTCAAG C^T[CT][GA]AG
SspBI 830 TGTACA T^GTACA
StyI 350 CCATGG C^C[AT][AT]GG
TfiI 796 GATTC G^A[AT]TC
Tru9I 890 TTAA T^TAA
Tsp509I 887 AATT ^AATT
TspRI 579 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 426 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 209 AGATCT [GA]^GATC[CT]
XmaCI 411 CCCGGG C^CCGGG
XmaI 411 CCCGGG C^CCGGG
XmnI 522 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AciI 363 CCGC CCGC(-3/-1)
385 CCGC
AviII 654 TGCGCA TGC^GCA
677 TGCGCA
BanII 189 GAGCTC G[GA]GC[CT]^C
804 GAGCTC
BbsI 748 GTCTTC GAAGAC(2/6)
953 GTCTTC
BfaI 205 CTAG C^TAG
950 CTAG
BpuAI 748 GTCTTC GAAGAC(2/6)
953 GTCTTC
BsaAI 217 TACGTG [CT]AC^GT[GA]
367 TACGTG
BseRI 85 CTCCTC GAGGAG(10/8)
256 CTCCTC
BspMI 74 ACCTGC ACCTGC(4/8)
718 ACCTGC
DdeI 187 CTGAG C^T[ACGT]AG
874 CTGAG
DpnII 210 GATC ^GATC
471 GATC
EarI 79 CTCTTC CTCTTC(1/4)
88 CTCTTC
FspI 654 TGCGCA TGC^GCA
677 TGCGCA
HgaI 294 GACGC GACGC(5/10)
494 GCGTC
Ksp632I 79 CTCTTC CTCTTC(1/4)
88 CTCTTC
MaeI 205 CTAG C^TAG
950 CTAG
MboI 210 GATC ^GATC
471 GATC
MspA1I 385 CCGCTG C[AC]G^C[GT]G
427 CAGCTG
NdeII 210 GATC ^GATC
471 GATC
PstI 67 CTGCAG CTGCA^G
581 CTGCAG
SacI 189 GAGCTC GAGCT^C
804 GAGCTC
Sau3AI 210 GATC ^GATC
471 GATC
SfcI 67 CTGCAG C^T[GA][CT]AG
581 CTGCAG
XcmI 548 CCATCCTCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
578 CCACTGCAGAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AfaI AluI AspHI BalI BbvI
BcnI BmyI BpmI BsaJI BsiHKAI
BsiYI BslI Bsp1286I BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DsaI
EaeI EcoRII Fnu4HI FokI HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MaeII MaeIII MboII MluNI
MnlI MscI MslI MspI MvaI
MwoI NciI NlaIII NlaIV RsaI
Sau96I ScrFI SfaNI TseI Tsp45I