Restriction map for
pFN21AE5479
(Circular) (Six-base) MAPSORT of: pFN21AE5479.seq Check: 3019 from: 1 to: 5698
pFN21AE5479 5698 bp
With 182 enzymes: SgfI *
January 30, 2014 17:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2732 2732
Size: 5698
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3508 278
Size: 53 83 186 936 1972 2468
Fragments arranged by size:
2468 1972 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3041 1937
Size: 1104 4594
Acc65I G'GTAC_C
Cuts at: 3019 3019
Size: 5698
AclI AA'CG_TT
Cuts at: 1563 3825 4198 1563
Size: 2262 373 3063
Fragments arranged by size:
3063 2262 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2299 3761 5145 5413 1632
Size: 667 1462 1384 268 1917
Fragments arranged by size:
1917 1462 1384 667 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5501
AflIII A'CryG_T
Cuts at: 1203 2223 4597 5574 1203
Size: 1020 2374 977 1327
Fragments arranged by size:
2374 1327 1020 977
AgeI A'CCGG_T
Cuts at: 4529 4529
Size: 5698
AhdI GACnn_n'nnGTC
Cuts at: 4427 4427
Size: 5698
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5698
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5698
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2958 2990 2958
Size: 32 5666
AlwNI CAG_nnn'CTG
Cuts at: 2080 3441 5013 2080
Size: 1361 1572 2765
Fragments arranged by size:
2765 1572 1361
ApaLI G'TGCA_C
Cuts at: 2329 3755 4911 2329
Size: 1426 1156 3116
Fragments arranged by size:
3116 1426 1156
ApoI r'AATT_y
Cuts at: 1493 2891 3007 3233 3385 5494 1493
Size: 1398 116 226 152 2109 1697
Fragments arranged by size:
2109 1697 1398 226 152 116
AseI AT'TA_AT
Cuts at: 160 1032 4252 160
Size: 872 3220 1606
Fragments arranged by size:
3220 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5698
AvaI C'yCGr_G
Cuts at: 1870 1943 2416 3023 1870
Size: 73 473 607 4545
Fragments arranged by size:
4545 607 473 73
BamHI G'GATC_C
Cuts at: 3028 3395 3028
Size: 367 5331
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2033 3019 4474 618
Size: 325 217 627 246 986 1455 1842
Fragments arranged by size:
1842 1455 986 627 325 246 217
BanII G_rGCy'C
Cuts at: 1391 2198 2813 3017 1391
Size: 807 615 204 4072
Fragments arranged by size:
4072 807 615 204
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2746 2951 928
Size: 971 847 205 3675
Fragments arranged by size:
3675 971 847 205
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2266 2300 3889 3923 1903
Size: 34 329 34 1589 34 3678
Fragments arranged by size:
3678 1589 329 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3590 4800 3590
Size: 1210 4488
BclI T'GATC_A
Cuts at: 1548 2475 1548
Size: 927 4771
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2357 4309 136
Size: 107 122 71 631 605 685 1952 1525
Fragments arranged by size:
1952 1525 685 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2214 5693 2214
Size: 3479 2219
BlpI GC'TnA_GC
Cuts at: 3115 3115
Size: 5698
Bme1580I G_kGCm'C
Cuts at: 1790 2333 3759 4915 1790
Size: 543 1426 1156 2573
Fragments arranged by size:
2573 1426 1156 543
BmgBI CAC'GTC
Cuts at: 1318 2404 1318
Size: 1086 4612
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3429 4387 442
Size: 416 53 83 399 24 2012 958 1753
Fragments arranged by size:
2012 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5698
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2173 2249 2270 2414 2479 2728 4358 1239
Size: 934 76 21 144 65 249 1630 2579
Fragments arranged by size:
2579 1630 934 249 144 76 65 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2543 3823 4688 4986 2543
Size: 1280 865 298 3255
Fragments arranged by size:
3255 1280 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4361 882
Size: 617 2862 2219
Fragments arranged by size:
2862 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2224 2374 3489 493
Size: 711 1020 150 1115 2702
Fragments arranged by size:
2702 1115 1020 711 150
BsaBI GATnn'nnATC
Cuts at: 5692 5692
Size: 5698
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3505 3887 4522 275
Size: 53 83 186 936 1972 382 635 1451
Fragments arranged by size:
1972 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4131 4529 4803 4950 5361 4131
Size: 398 274 147 411 4468
Fragments arranged by size:
4468 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2958 2988 1178
Size: 30 165 30 1555 30 3888
Fragments arranged by size:
3888 1555 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2081 2252 1186
Size: 895 171 4632
Fragments arranged by size:
4632 895 171
BseYI C'CCAG_C
Cuts at: 4901 5397 4901
Size: 496 5202
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2554 1163
Size: 573 818 4307
Fragments arranged by size:
4307 818 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2368 3148 3909 4937 1540
Size: 275 186 367 780 761 1028 2301
Fragments arranged by size:
2301 1028 780 761 367 275 186
BsiHKAI G_wGCw'C
Cuts at: 2198 2333 2813 3759 3844 4915 2198
Size: 135 480 946 85 1071 2981
Fragments arranged by size:
2981 1071 946 480 135 85
BsmI GAATG_Cn'
Cuts at: 2905 3209 3302 2905
Size: 304 93 5301
Fragments arranged by size:
5301 304 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2198 2333 2813 3017 3759 3844 4915
Size: 399 408 135 480 204 742 85 1071
Cuts at: 4915 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 480 408 399 204 135
85
BspEI T'CCGG_A
Cuts at: 5361 5361
Size: 5698
BspHI T'CATG_A
Cuts at: 3587 5317 3587
Size: 1730 3968
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2088 2732 3053 844
Size: 1244 644 321 3489
Fragments arranged by size:
3489 1244 644 321
BsrBI CCG'CTC
Cuts at: 1394 1823 3585 1394
Size: 429 1762 3507
Fragments arranged by size:
3507 1762 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4193 4367 65
Size: 1183 2945 174 1396
Fragments arranged by size:
2945 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2699 4342 4529 1700
Size: 110 889 1643 187 2869
Fragments arranged by size:
2869 1643 889 187 110
BsrGI T'GTAC_A
Cuts at: 96 2835 96
Size: 2739 2959
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5698
BssSI C'ACGA_G
Cuts at: 3758 4770 3758
Size: 1012 4686
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2080 794
Size: 1286 4412
BstBI TT'CG_AA
Cuts at: 4502 4502
Size: 5698
BstEII G'GTnAC_C
Cuts at: 1184 2746 1184
Size: 1562 4136
BstXI CCAn_nnnn'nTGG
Cuts at: 2248 2248
Size: 5698
BstYI r'GATC_y
Cuts at: 1972 2214 3028 3395 3780 3797 5249 5335 5347
Size: 242 814 367 385 17 1452 86 12
Cuts at: 5347 5693 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 814 385 367 346 242 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2879 2879
Size: 5698
BtgI C'CryG_G
Cuts at: 513 1065 2058 2355 2742 513
Size: 552 993 297 387 3469
Fragments arranged by size:
3469 993 552 387 297
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4959
Fragments arranged by size:
4959 683 56
BtsI GCAGTG_nn'
Cuts at: 2583 3214 4008 4028 2583
Size: 631 794 20 4253
Fragments arranged by size:
4253 794 631 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5698
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5663
DraI TTT'AAA
Cuts at: 3002 3002
Size: 5698
DraIII CAC_nnn'GTG
Cuts at: 2337 2337
Size: 5698
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4705 809
Size: 507 3389 1802
Fragments arranged by size:
3389 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2133 2376 2640
Size: 54 1425 50 275 18 303 243 264
Cuts at: 2640 2694 3145 4034 5663 8
Size: 54 451 889 1629 43
Fragments arranged by size:
1629 1425 889 451 303 275 264 243
54 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3145 1537
Size: 275 1333 4090
Fragments arranged by size:
4090 1333 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2090 2099 3628 1353
Size: 737 9 1529 3423
Fragments arranged by size:
3423 1529 737 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4285 4659 4805 387
Size: 1064 138 2696 374 146 1280
Fragments arranged by size:
2696 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2196 2811 2196
Size: 615 5083
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2173 2249 2270 2299 2414 2479 2728
Size: 393 541 76 21 29 115 65 249
Cuts at: 2728 3761 4358 5145 5413 1239
Size: 1033 597 787 268 1524
Fragments arranged by size:
1524 1033 787 597 541 393 268 249
115 76 65 29 21
EcoRI G'AATT_C
Cuts at: 3007 3385 3007
Size: 378 5320
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2920 2920
Size: 5698
FspI TGC'GCA
Cuts at: 2661 2684 4204 2661
Size: 23 1520 4155
Fragments arranged by size:
4155 1520 23
FspAI rTGC'GCAy
Cuts at: 2661 2661
Size: 5698
HaeII r_GCGC'y
Cuts at: 1678 4845 1678
Size: 3167 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4339 4371 4413 4445 1373
Size: 32 135 32 2767 32 42 32 2626
Fragments arranged by size:
2767 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3042 3288 669
Size: 988 281 1104 246 3079
Fragments arranged by size:
3079 1104 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5698
HpaI GTT'AAC
Cuts at: 3288 3288
Size: 5698
KpnI G_GTAC'C
Cuts at: 3023 3023
Size: 5698
MfeI C'AATT_G
Cuts at: 3297 3297
Size: 5698
MluI A'CGCG_T
Cuts at: 5574 5574
Size: 5698
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2541 2838 4812 4996 1305
Size: 1236 297 1974 184 2007
Fragments arranged by size:
2007 1974 1236 297 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2135 2378 2642 10
Size: 54 1425 343 303 243 264 3066
Fragments arranged by size:
3066 1425 343 303 264 243 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2045 2471 2624 3656 4015 4174 518
Size: 648 879 426 153 1032 359 159 2042
Fragments arranged by size:
2042 1032 879 648 426 359 159 153
MspA1I CmG'CkG
Cuts at: 1606 2392 2434 3114 3791 4939 5184 1606
Size: 786 42 680 677 1148 245 2120
Fragments arranged by size:
2120 1148 786 680 677 245 42
NcoI C'CATG_G
Cuts at: 513 1065 2355 513
Size: 552 1290 3856
Fragments arranged by size:
3856 1290 552
NdeI CA'TA_TG
Cuts at: 387 2656 387
Size: 2269 3429
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5698
NotI GC'GGCC_GC
Cuts at: 3145 3145
Size: 5698
NspI r_CATG'y
Cuts at: 1132 1229 3056 4601 1132
Size: 97 1827 1545 2229
Fragments arranged by size:
2229 1827 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2066 1348
Size: 718 4980
PciI A'CATG_T
Cuts at: 4597 4597
Size: 5698
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2437 1108
Size: 1329 4369
PfoI T'CCnGG_A
Cuts at: 2804 2804
Size: 5698
PmeI GTTT'AAAC
Cuts at: 3002 3002
Size: 5698
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2958 2990 3767 3799 5280 5312 2958
Size: 32 777 32 1481 32 3344
Fragments arranged by size:
3344 1481 777 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5698
PsiI TTA'TAA
Cuts at: 3268 3268
Size: 5698
PstI C_TGCA'G
Cuts at: 830 2076 2590 3050 830
Size: 1246 514 460 3478
Fragments arranged by size:
3478 1246 514 460
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5698
PvuII CAG'CTG
Cuts at: 2434 2434
Size: 5698
SacI G_AGCT'C
Cuts at: 2198 2813 2198
Size: 615 5083
SalI G'TCGA_C
Cuts at: 1936 3040 1936
Size: 1104 4594
SapI GCTCTTCn'nnn_
Cuts at: 2090 2090
Size: 5698
SbfI CC_TGCA'GG
Cuts at: 3050 3050
Size: 5698
ScaI AGT'ACT
Cuts at: 3946 5658 3946
Size: 1712 3986
SfcI C'TryA_G
Cuts at: 826 1045 2072 2586 3046 4181 4862 5053 826
Size: 219 1027 514 460 1135 681 191 1471
Fragments arranged by size:
1471 1135 1027 681 514 460 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2357 2357
Size: 5698
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5698
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5698
SmaI CCC'GGG
Cuts at: 1872 2418 3025 1872
Size: 546 607 4545
Fragments arranged by size:
4545 607 546
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2558 3802 4703 4965 820
Size: 197 926 615 1244 901 262 1553
Fragments arranged by size:
1553 1244 926 901 615 262 197
SnaBI TAC'GTA
Cuts at: 493 3489 493
Size: 2996 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5698
SphI G_CATG'C
Cuts at: 1132 3056 1132
Size: 1924 3774
SspI AAT'ATT
Cuts at: 5 52 3622 5
Size: 47 3570 2081
Fragments arranged by size:
3570 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5698
StyI C'CwwG_G
Cuts at: 513 1065 2355 3137 513
Size: 552 1290 782 3074
Fragments arranged by size:
3074 1290 782 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3725 3742 3895 4080 4525 325
Size: 1255 2145 17 153 185 445 1498
Fragments arranged by size:
2145 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2181 2835 3944
Size: 274 80 33 51 1443 204 654 1109
Cuts at: 3944 5656 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1109 654 274 204 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5658 5690 5658
Size: 32 5666
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5698
XbaI T'CTAG_A
Cuts at: 3034 3034
Size: 5698
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2560 2590 2560
Size: 30 5668
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5698
XmaI C'CCGG_G
Cuts at: 1870 2416 3023 1870
Size: 546 607 4545
Fragments arranged by size:
4545 607 546
XmnI GAAnn'nnTTC
Cuts at: 2531 3827 2531
Size: 1296 4402
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3506 276
Size: 53 83 186 936 1972 2468
Fragments arranged by size:
2468 1972 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I EcoNI EcoO109I EcoRV
FseI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI
SexAI SfoI SrfI SwaI