Restriction map for pFN21AE5284
 (Linear) (Six-base) MAPSORT of: KIEE5284.seq  Check: 9362  from: 1  to: 630



KIEE5284.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:54  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     513     630 
   Size:        513     117
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     166     630 
   Size:        166     464
 
AleI CACnn'nnGTG

Cuts at:      0     305     630 
   Size:        305     325
 
AlwNI CAG_nnn'CTG

Cuts at:      0     246     471     630 
   Size:        246     225     159

  Fragments arranged by size: 

                 246     225     159
 
ApaI G_GGCC'C

Cuts at:      0     483     513     630 
   Size:        483      30     117

  Fragments arranged by size: 

                 483     117      30
 
AvaI C'yCGr_G

Cuts at:      0     129     482     630 
   Size:        129     353     148

  Fragments arranged by size: 

                 353     148     129
 
BanII G_rGCy'C

Cuts at:      0     483     513     630 
   Size:        483      30     117

  Fragments arranged by size: 

                 483     117      30
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     154     630 
   Size:        154     476
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     120     154     564     598     630 
   Size:        120      34     410      34      32

  Fragments arranged by size: 

                 410     120      34      34      32
 
BglII A'GATC_T

Cuts at:      0     119     378     392     630 
   Size:        119     259      14     238

  Fragments arranged by size: 

                 259     238     119      14
 
Bme1580I G_kGCm'C

Cuts at:      0     483     513     630 
   Size:        483      30     117

  Fragments arranged by size: 

                 483     117      30
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     202     268     630 
   Size:        202      66     362

  Fragments arranged by size: 

                 362     202      66
 
Bpu10I CC'TnA_GC

Cuts at:      0     488     630 
   Size:        488     142
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     502     630 
   Size:        502     128
 
BsaHI Gr'CG_yC

Cuts at:      0      60     630 
   Size:         60     570
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      57     211     630 
   Size:         57     154     419

  Fragments arranged by size: 

                 419     154      57
 
BseYI C'CCAG_C

Cuts at:      0     261     474     630 
   Size:        261     213     156

  Fragments arranged by size: 

                 261     213     156
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      26     630 
   Size:         26     604
 
BsiHKAI G_wGCw'C

Cuts at:      0     232     630 
   Size:        232     398
 
Bsp1286I G_dGCh'C

Cuts at:      0     232     483     513     630 
   Size:        232     251      30     117

  Fragments arranged by size: 

                 251     232     117      30
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     512     513     630 
   Size:        512       1     117

  Fragments arranged by size: 

                 512     117       1
 
BsrBI CCG'CTC

Cuts at:      0      65     201     630 
   Size:         65     136     429

  Fragments arranged by size: 

                 429     136      65
 
BssHII G'CGCG_C

Cuts at:      0     219     630 
   Size:        219     411
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     236     471     630 
   Size:        236     235     159

  Fragments arranged by size: 

                 236     235     159
 
BstYI r'GATC_y

Cuts at:      0     119     378     392     443     630 
   Size:        119     259      14      51     187

  Fragments arranged by size: 

                 259     187     119      51      14
 
BtgI C'CryG_G

Cuts at:      0      89     630 
   Size:         89     541
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      32     630 
   Size:         32     598
 
ClaI AT'CG_AT

Cuts at:      0     584     630 
   Size:        584      46
 
DraIII CAC_nnn'GTG

Cuts at:      0     595     630 
   Size:        595      35
 
EaeI y'GGCC_r

Cuts at:      0     254     630 
   Size:        254     376
 
EarI CTCTTCn'nnn_

Cuts at:      0     320     630 
   Size:        320     310
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19     114     208     630 
   Size:         19      95      94     422

  Fragments arranged by size: 

                 422      95      94      19
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     166     202     268     630 
   Size:        166      36      66     362

  Fragments arranged by size: 

                 362     166      66      36
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     520     630 
   Size:        520     110
 
EcoO109I rG'GnC_Cy

Cuts at:      0     479     630 
   Size:        479     151
 
HaeII r_GCGC'y

Cuts at:      0     472     630 
   Size:        472     158
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     363     395     428     460     630 
   Size:        363      32      33      32     170

  Fragments arranged by size: 

                 363     170      33      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      95     630 
   Size:         95     535
 
MslI CAynn'nnrTG

Cuts at:      0     305     630 
   Size:        305     325
 
MspA1I CmG'CkG

Cuts at:      0     144     236     246     630 
   Size:        144      92      10     384

  Fragments arranged by size: 

                 384     144      92      10
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     246     630 
   Size:        246     384
 
PspOMI G'GGCC_C

Cuts at:      0     479     509     630 
   Size:        479      30     121

  Fragments arranged by size: 

                 479     121      30
 
PstI C_TGCA'G

Cuts at:      0     164     281     453     630 
   Size:        164     117     172     177

  Fragments arranged by size: 

                 177     172     164     117
 
PvuII CAG'CTG

Cuts at:      0     144     630 
   Size:        144     486
 
SfcI C'TryA_G

Cuts at:      0     160     277     449     630 
   Size:        160     117     172     181

  Fragments arranged by size: 

                 181     172     160     117
 
SmaI CCC'GGG

Cuts at:      0     484     630 
   Size:        484     146
 
SmlI C'TyrA_G

Cuts at:      0     129     517     630 
   Size:        129     388     113

  Fragments arranged by size: 

                 388     129     113
 
SrfI GCCC'GGGC

Cuts at:      0     484     630 
   Size:        484     146
 
XhoI C'TCGA_G

Cuts at:      0     129     630 
   Size:        129     501
 
XmaI C'CCGG_G

Cuts at:      0     482     630 
   Size:        482     148
 

 Enzymes that do cut:

     AarI     AcuI     AleI    AlwNI     ApaI     AvaI    BanII     BbsI
     BcgI    BglII Bme1580I     BpmI   Bpu10I    BpuEI    BsaHI    BseRI
    BseYI     BsgI  BsiHKAI Bsp1286I    BspMI    BsrBI   BssHII   BstAPI
    BstYI     BtgI    BtgZI     ClaI   DraIII     EaeI     EarI     EciI
  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I     MmeI     MslI   MspA1I
    PflMI   PspOMI     PstI    PvuII     SfcI     SmaI     SmlI     SrfI
     XhoI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI    BciVI     BclI
    BfrBI     BglI     BlpI    BmgBI     BmrI     BmtI     BplI     BsaI
    BsaAI    BsaBI    BsaWI    BsaXI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BsrDI    BsrFI    BsrGI    BssSI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtsI    CspCI     DraI     DrdI     EagI
  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SspI     StuI
     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XmnI     ZraI
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