(Circular) (Six-base) MAPSORT of: pFN21AE9577.seq Check: 5864 from: 1 to: 6334 pFN21AE9577 6334 bp With 182 enzymes: SgfI * January 30, 2014 17:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3018 3391 3018 Size: 373 5961 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4144 278 Size: 53 83 186 936 2608 2468 Fragments arranged by size: 2608 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3369 3677 1937 Size: 1432 308 4594 Fragments arranged by size: 4594 1432 308 Acc65I G'GTAC_C Cuts at: 3655 3655 Size: 6334 AclI AA'CG_TT Cuts at: 1563 4461 4834 1563 Size: 2898 373 3063 Fragments arranged by size: 3063 2898 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2602 3035 3347 4397 5781 6049 1632 Size: 970 433 312 1050 1384 268 1917 Fragments arranged by size: 1917 1384 1050 970 433 312 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6137 AflIII A'CryG_T Cuts at: 1203 2859 5233 6210 1203 Size: 1656 2374 977 1327 Fragments arranged by size: 2374 1656 1327 977 AgeI A'CCGG_T Cuts at: 5165 5165 Size: 6334 AhdI GACnn_n'nnGTC Cuts at: 5063 5063 Size: 6334 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6334 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6334 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3194 3226 3563 3595 3194 Size: 32 337 32 5933 Fragments arranged by size: 5933 337 32 32 AlwNI CAG_nnn'CTG Cuts at: 3133 3430 4077 5649 3133 Size: 297 647 1572 3818 Fragments arranged by size: 3818 1572 647 297 ApaLI G'TGCA_C Cuts at: 4391 5547 4391 Size: 1156 5178 ApoI r'AATT_y Cuts at: 1493 3643 3869 4021 6130 1493 Size: 2150 226 152 2109 1697 Fragments arranged by size: 2150 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4888 160 Size: 872 3856 1606 Fragments arranged by size: 3856 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6334 AvaI C'yCGr_G Cuts at: 1870 1943 2298 2426 3659 1870 Size: 73 355 128 1233 4545 Fragments arranged by size: 4545 1233 355 128 73 BamHI G'GATC_C Cuts at: 3664 4031 3664 Size: 367 5967 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3020 3176 3236 3655 5110 Size: 325 217 627 1233 156 60 419 1455 Cuts at: 5110 618 Size: 1842 Fragments arranged by size: 1842 1455 1233 627 419 325 217 156 60 BanII G_rGCy'C Cuts at: 1391 2031 2045 2433 2463 3455 3653 1391 Size: 640 14 388 30 992 198 4072 Fragments arranged by size: 4072 992 640 388 198 30 14 BbeI G_GCGC'C Cuts at: 3024 3180 3024 Size: 156 6178 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2414 2863 928 Size: 971 515 449 4399 Fragments arranged by size: 4399 971 515 449 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2966 3000 4525 4559 1903 Size: 34 1029 34 1525 34 3678 Fragments arranged by size: 3678 1525 1029 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4226 5436 4226 Size: 1210 5124 BclI T'GATC_A Cuts at: 1548 1548 Size: 6334 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3143 4945 136 Size: 107 122 71 631 605 1471 1802 1525 Fragments arranged by size: 1802 1525 1471 631 605 122 107 71 BglII A'GATC_T Cuts at: 6329 6329 Size: 6334 BlpI GC'TnA_GC Cuts at: 3751 3751 Size: 6334 Bme1580I G_kGCm'C Cuts at: 1790 3414 4395 5551 1790 Size: 1624 981 1156 2573 Fragments arranged by size: 2573 1624 1156 981 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6334 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4065 5023 442 Size: 416 53 83 399 24 2648 958 1753 Fragments arranged by size: 2648 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6334 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2937 2937 Size: 6334 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2204 2306 3260 4994 1239 Size: 965 102 954 1734 2579 Fragments arranged by size: 2579 1734 965 954 102 Bpu10I CC'TnA_GC Cuts at: 2591 3055 2591 Size: 464 5870 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4459 5324 5622 4459 Size: 865 298 5171 Fragments arranged by size: 5171 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4997 882 Size: 617 3498 2219 Fragments arranged by size: 3498 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2629 2862 3190 4125 493 Size: 711 1425 233 328 935 2702 Fragments arranged by size: 2702 1425 935 711 328 233 BsaBI GATnn'nnATC Cuts at: 3116 6328 3116 Size: 3212 3122 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3021 3177 4141 4523 Size: 53 83 186 936 1488 156 964 382 Cuts at: 4523 5158 275 Size: 635 1451 Fragments arranged by size: 1488 1451 964 936 635 382 186 156 83 53 BsaWI w'CCGG_w Cuts at: 4767 5165 5439 5586 5997 4767 Size: 398 274 147 411 5104 Fragments arranged by size: 5104 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2177 2207 2445 2475 3194 Size: 30 165 30 774 30 238 30 719 Cuts at: 3194 3224 1178 Size: 30 4288 Fragments arranged by size: 4288 774 719 238 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2035 2423 2915 3017 3554 1186 Size: 849 388 492 102 537 3966 Fragments arranged by size: 3966 849 537 492 388 102 BseYI C'CCAG_C Cuts at: 2209 3060 5537 6033 2209 Size: 851 2477 496 2510 Fragments arranged by size: 2510 2477 851 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5761 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2035 3784 4545 5573 1540 Size: 275 186 34 1749 761 1028 2301 Fragments arranged by size: 2301 1749 1028 761 275 186 34 BsiHKAI G_wGCw'C Cuts at: 3477 4395 4480 5551 3477 Size: 918 85 1071 4260 Fragments arranged by size: 4260 1071 918 85 BsmI GAATG_Cn' Cuts at: 3845 3938 3845 Size: 93 6241 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2031 2045 2433 2463 3414 3455 3477 Size: 399 241 14 388 30 951 41 22 Cuts at: 3477 3653 4395 4480 5551 1391 Size: 176 742 85 1071 2174 Fragments arranged by size: 2174 1071 951 742 399 388 241 176 85 41 30 22 14 BspEI T'CCGG_A Cuts at: 5997 5997 Size: 6334 BspHI T'CATG_A Cuts at: 4223 5953 4223 Size: 1730 4604 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2368 3018 3118 3391 3525 3689 844 Size: 1524 650 100 273 134 164 3489 Fragments arranged by size: 3489 1524 650 273 164 134 100 BsrBI CCG'CTC Cuts at: 1394 1823 2138 4221 1394 Size: 429 315 2083 3507 Fragments arranged by size: 3507 2083 429 315 BsrDI GCAATG_nn' Cuts at: 65 1248 4829 5003 65 Size: 1183 3581 174 1396 Fragments arranged by size: 3581 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2109 4978 5165 1700 Size: 110 299 2869 187 2869 Fragments arranged by size: 2869 2869 299 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6334 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6334 BssSI C'ACGA_G Cuts at: 4394 5406 4394 Size: 1012 5322 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6334 BstBI TT'CG_AA Cuts at: 5138 5138 Size: 6334 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6334 BstYI r'GATC_y Cuts at: 1972 2371 2877 3664 4031 4416 4433 5885 5971 Size: 399 506 787 367 385 17 1452 86 Cuts at: 5971 5983 6329 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 787 506 399 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2035 2046 513 Size: 552 939 31 11 4801 Fragments arranged by size: 4801 939 552 31 11 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2986 3025 3370 487 Size: 683 56 1760 39 345 3451 Fragments arranged by size: 3451 1760 683 345 56 39 BtsI GCAGTG_nn' Cuts at: 2016 2550 3315 3431 3850 4644 4664 2016 Size: 534 765 116 419 794 20 3686 Fragments arranged by size: 3686 794 765 534 419 116 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6334 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6299 DraI TTT'AAA Cuts at: 3638 3638 Size: 6334 DraIII CAC_nnn'GTG Cuts at: 2632 2632 Size: 6334 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5341 809 Size: 507 4025 1802 Fragments arranged by size: 4025 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2032 2820 3135 Size: 54 1425 50 275 18 202 788 315 Cuts at: 3135 3318 3781 4670 6299 8 Size: 183 463 889 1629 43 Fragments arranged by size: 1629 1425 889 788 463 315 275 202 183 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2032 3781 1537 Size: 275 220 1749 4090 Fragments arranged by size: 4090 1749 275 220 EarI CTCTTCn'nnn_ Cuts at: 1353 2223 2933 3035 4264 1353 Size: 870 710 102 1229 3423 Fragments arranged by size: 3423 1229 870 710 102 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2517 2663 2797 4921 5295 5441 Size: 1064 138 928 146 134 2124 374 146 Cuts at: 5441 387 Size: 1280 Fragments arranged by size: 2124 1280 1064 928 374 146 146 138 134 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2204 2306 2602 3035 3260 3347 4397 Size: 393 572 102 296 433 225 87 1050 Cuts at: 4397 4994 5781 6049 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1050 787 597 572 433 393 296 268 225 102 87 EcoNI CCTnn'n_nnAGG Cuts at: 2219 3014 3398 2219 Size: 795 384 5155 Fragments arranged by size: 5155 795 384 EcoO109I rG'GnC_Cy Cuts at: 2508 2555 2575 3130 2508 Size: 47 20 555 5712 Fragments arranged by size: 5712 555 47 20 EcoRI G'AATT_C Cuts at: 3643 4021 3643 Size: 378 5956 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3121 3121 Size: 6334 FspI TGC'GCA Cuts at: 4840 4840 Size: 6334 HaeII r_GCGC'y Cuts at: 1678 2120 3024 3180 5481 1678 Size: 442 904 156 2301 2531 Fragments arranged by size: 2531 2301 904 442 156 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2127 2159 2539 2571 2626 Size: 32 135 32 555 32 380 32 55 Cuts at: 2626 2658 4975 5007 5049 5081 1373 Size: 32 2317 32 42 32 2626 Fragments arranged by size: 2626 2317 555 380 135 55 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2535 3604 3678 3924 669 Size: 988 281 597 1069 74 246 3079 Fragments arranged by size: 3079 1069 988 597 281 246 74 HindIII A'AGCT_T Cuts at: 748 748 Size: 6334 HpaI GTT'AAC Cuts at: 3924 3924 Size: 6334 KasI G'GCGC_C Cuts at: 3020 3176 3020 Size: 156 6178 KpnI G_GTAC'C Cuts at: 3659 3659 Size: 6334 MfeI C'AATT_G Cuts at: 3933 3933 Size: 6334 MluI A'CGCG_T Cuts at: 6210 6210 Size: 6334 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3066 5448 5632 1305 Size: 1761 2382 184 2007 Fragments arranged by size: 2382 2007 1761 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3137 3320 10 Size: 54 1425 343 1305 183 3024 Fragments arranged by size: 3024 1425 1305 343 183 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2726 2729 2801 3345 4292 4651 Size: 648 843 717 3 72 544 947 359 Cuts at: 4651 4810 518 Size: 159 2042 Fragments arranged by size: 2042 947 843 717 648 544 359 159 72 3 MspA1I CmG'CkG Cuts at: 1606 2037 2048 2062 2149 2771 2825 3523 3618 Size: 431 11 14 87 622 54 698 95 Cuts at: 3618 3750 4427 5575 5820 1606 Size: 132 677 1148 245 2120 Fragments arranged by size: 2120 1148 698 677 622 431 245 132 95 87 54 14 11 NarI GG'CG_CC Cuts at: 3021 3177 3021 Size: 156 6178 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4843 Fragments arranged by size: 4843 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6334 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6334 NotI GC'GGCC_GC Cuts at: 3781 3781 Size: 6334 NspI r_CATG'y Cuts at: 1132 1229 3692 5237 1132 Size: 97 2463 1545 2229 Fragments arranged by size: 2463 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6334 PciI A'CATG_T Cuts at: 5233 5233 Size: 6334 PflMI CCAn_nnn'nTGG Cuts at: 1108 2113 3442 1108 Size: 1005 1329 4000 Fragments arranged by size: 4000 1329 1005 PfoI T'CCnGG_A Cuts at: 2217 2307 2436 2736 3276 3582 2217 Size: 90 129 300 540 306 4969 Fragments arranged by size: 4969 540 306 300 129 90 PmeI GTTT'AAAC Cuts at: 3638 3638 Size: 6334 PmlI CAC'GTG Cuts at: 2629 2629 Size: 6334 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3194 3226 4403 4435 5916 5948 3194 Size: 32 1177 32 1481 32 3580 Fragments arranged by size: 3580 1481 1177 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2508 2555 2575 3130 2508 Size: 47 20 555 5712 Fragments arranged by size: 5712 555 47 20 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6334 PsiI TTA'TAA Cuts at: 3904 3904 Size: 6334 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3497 3529 3497 Size: 32 6302 PstI C_TGCA'G Cuts at: 830 2379 2518 2855 3402 3522 3686 830 Size: 1549 139 337 547 120 164 3478 Fragments arranged by size: 3478 1549 547 337 164 139 120 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6334 PvuII CAG'CTG Cuts at: 2149 3523 2149 Size: 1374 4960 SacII CC_GC'GG Cuts at: 2038 2049 2038 Size: 11 6323 SalI G'TCGA_C Cuts at: 1936 3676 1936 Size: 1740 4594 SbfI CC_TGCA'GG Cuts at: 3686 3686 Size: 6334 ScaI AGT'ACT Cuts at: 4582 6294 4582 Size: 1712 4622 SexAI A'CCwGG_T Cuts at: 2577 2577 Size: 6334 SfcI C'TryA_G Cuts at: 826 1045 2375 2514 2851 3398 3518 3682 4817 Size: 219 1330 139 337 547 120 164 1135 Cuts at: 4817 5498 5689 826 Size: 681 191 1471 Fragments arranged by size: 1471 1330 1135 681 547 337 219 191 164 139 120 SfiI GGCCn_nnn'nGGCC Cuts at: 3143 3143 Size: 6334 SfoI GGC'GCC Cuts at: 3022 3178 3022 Size: 156 6178 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6334 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6334 SmaI CCC'GGG Cuts at: 1872 2300 2428 3661 1872 Size: 428 128 1233 4545 Fragments arranged by size: 4545 1233 428 128 SmlI C'TyrA_G Cuts at: 820 1017 1943 4438 5339 5601 820 Size: 197 926 2495 901 262 1553 Fragments arranged by size: 2495 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4125 493 Size: 3632 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6334 SphI G_CATG'C Cuts at: 1132 3692 1132 Size: 2560 3774 SspI AAT'ATT Cuts at: 5 52 4258 5 Size: 47 4206 2081 Fragments arranged by size: 4206 2081 47 StuI AGG'CCT Cuts at: 1517 2982 3591 1517 Size: 1465 609 4260 Fragments arranged by size: 4260 1465 609 StyI C'CwwG_G Cuts at: 513 1065 2004 2367 2898 3773 513 Size: 552 939 363 531 875 3074 Fragments arranged by size: 3074 939 875 552 531 363 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2075 2259 2827 4361 4378 4531 4716 Size: 1255 495 184 568 1534 17 153 185 Cuts at: 4716 5161 325 Size: 445 1498 Fragments arranged by size: 1534 1498 1255 568 495 445 185 184 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2661 2994 4580 Size: 274 80 33 51 1443 684 333 1586 Cuts at: 4580 6292 96 Size: 1712 138 Fragments arranged by size: 1712 1586 1443 684 333 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6294 6326 6294 Size: 32 6302 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6334 XbaI T'CTAG_A Cuts at: 3670 3670 Size: 6334 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2179 3130 3284 2179 Size: 951 154 5229 Fragments arranged by size: 5229 951 154 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6334 XmaI C'CCGG_G Cuts at: 1870 2298 2426 3659 1870 Size: 428 128 1233 4545 Fragments arranged by size: 4545 1233 428 128 XmnI GAAnn'nnTTC Cuts at: 2156 3332 4463 2156 Size: 1176 1131 4027 Fragments arranged by size: 4027 1176 1131 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4142 276 Size: 53 83 186 936 2608 2468 Fragments arranged by size: 2608 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PspOMI PspXI RsrII SacI SanDI SapI SrfI SwaI