(Linear) (Six-base) MAPSORT of: KIEE9577.seq Check: 3351 from: 1 to: 1629 KIEE9577.orf With 182 enzymes: SgfI * January 29, 2014 12:07 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1013 1386 1629 Size: 1013 373 243 Fragments arranged by size: 1013 373 243 AccI GT'mk_AC Cuts at: 0 1364 1629 Size: 1364 265 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 597 1030 1342 1629 Size: 597 433 312 287 Fragments arranged by size: 597 433 312 287 AflIII A'CryG_T Cuts at: 0 854 1629 Size: 854 775 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1189 1221 1558 1590 1629 Size: 1189 32 337 32 39 Fragments arranged by size: 1189 337 39 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 1128 1425 1629 Size: 1128 297 204 Fragments arranged by size: 1128 297 204 AvaI C'yCGr_G Cuts at: 0 293 421 1629 Size: 293 128 1208 Fragments arranged by size: 1208 293 128 BanI G'GyrC_C Cuts at: 0 1015 1171 1231 1629 Size: 1015 156 60 398 Fragments arranged by size: 1015 398 156 60 BanII G_rGCy'C Cuts at: 0 26 40 428 458 1450 1629 Size: 26 14 388 30 992 179 Fragments arranged by size: 992 388 179 30 26 14 BbeI G_GCGC'C Cuts at: 0 1019 1175 1629 Size: 1019 156 454 Fragments arranged by size: 1019 454 156 BbsI GAAGACnn'nnnn_ Cuts at: 0 409 858 1629 Size: 409 449 771 Fragments arranged by size: 771 449 409 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 961 995 1629 Size: 961 34 634 Fragments arranged by size: 961 634 34 BglI GCCn_nnn'nGGC Cuts at: 0 1138 1629 Size: 1138 491 Bme1580I G_kGCm'C Cuts at: 0 1409 1629 Size: 1409 220 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 932 1629 Size: 932 697 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 199 301 1255 1629 Size: 199 102 954 374 Fragments arranged by size: 954 374 199 102 Bpu10I CC'TnA_GC Cuts at: 0 586 1050 1629 Size: 586 464 579 Fragments arranged by size: 586 579 464 BsaAI yAC'GTr Cuts at: 0 624 857 1185 1629 Size: 624 233 328 444 Fragments arranged by size: 624 444 328 233 BsaBI GATnn'nnATC Cuts at: 0 1111 1629 Size: 1111 518 BsaHI Gr'CG_yC Cuts at: 0 1016 1172 1629 Size: 1016 156 457 Fragments arranged by size: 1016 457 156 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 172 202 440 470 1189 1219 1629 Size: 172 30 238 30 719 30 410 Fragments arranged by size: 719 410 238 172 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 30 418 910 1012 1549 1629 Size: 30 388 492 102 537 80 Fragments arranged by size: 537 492 388 102 80 30 BseYI C'CCAG_C Cuts at: 0 204 1055 1629 Size: 204 851 574 Fragments arranged by size: 851 574 204 BsiEI CG_ry'CG Cuts at: 0 30 1629 Size: 30 1599 BsiHKAI G_wGCw'C Cuts at: 0 1472 1629 Size: 1472 157 Bsp1286I G_dGCh'C Cuts at: 0 26 40 428 458 1409 1450 1472 1629 Size: 26 14 388 30 951 41 22 157 Fragments arranged by size: 951 388 157 41 30 26 22 14 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 363 1013 1113 1386 1520 1629 Size: 363 650 100 273 134 109 Fragments arranged by size: 650 363 273 134 109 100 BsrBI CCG'CTC Cuts at: 0 133 1629 Size: 133 1496 BsrFI r'CCGG_y Cuts at: 0 104 1629 Size: 104 1525 BstYI r'GATC_y Cuts at: 0 366 872 1629 Size: 366 506 757 Fragments arranged by size: 757 506 366 BtgI C'CryG_G Cuts at: 0 30 41 1629 Size: 30 11 1588 Fragments arranged by size: 1588 30 11 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 981 1020 1365 1629 Size: 981 39 345 264 Fragments arranged by size: 981 345 264 39 BtsI GCAGTG_nn' Cuts at: 0 11 545 1310 1426 1629 Size: 11 534 765 116 203 Fragments arranged by size: 765 534 203 116 11 DraIII CAC_nnn'GTG Cuts at: 0 627 1629 Size: 627 1002 EaeI y'GGCC_r Cuts at: 0 27 815 1130 1313 1629 Size: 27 788 315 183 316 Fragments arranged by size: 788 316 315 183 27 EagI C'GGCC_G Cuts at: 0 27 1629 Size: 27 1602 EarI CTCTTCn'nnn_ Cuts at: 0 218 928 1030 1629 Size: 218 710 102 599 Fragments arranged by size: 710 599 218 102 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 512 658 792 1629 Size: 512 146 134 837 Fragments arranged by size: 837 512 146 134 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 199 301 597 1030 1255 1342 1629 Size: 199 102 296 433 225 87 287 Fragments arranged by size: 433 296 287 225 199 102 87 EcoNI CCTnn'n_nnAGG Cuts at: 0 214 1009 1393 1629 Size: 214 795 384 236 Fragments arranged by size: 795 384 236 214 EcoO109I rG'GnC_Cy Cuts at: 0 503 550 570 1125 1629 Size: 503 47 20 555 504 Fragments arranged by size: 555 504 503 47 20 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1116 1629 Size: 1116 513 HaeII r_GCGC'y Cuts at: 0 115 1019 1175 1629 Size: 115 904 156 454 Fragments arranged by size: 904 454 156 115 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 122 154 534 566 621 653 1629 Size: 122 32 380 32 55 32 976 Fragments arranged by size: 976 380 122 55 32 32 32 HincII GTy'rAC Cuts at: 0 530 1599 1629 Size: 530 1069 30 Fragments arranged by size: 1069 530 30 KasI G'GCGC_C Cuts at: 0 1015 1171 1629 Size: 1015 156 458 Fragments arranged by size: 1015 458 156 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1061 1629 Size: 1061 568 MscI TGG'CCA Cuts at: 0 1132 1315 1629 Size: 1132 183 314 Fragments arranged by size: 1132 314 183 MslI CAynn'nnrTG Cuts at: 0 721 724 796 1340 1629 Size: 721 3 72 544 289 Fragments arranged by size: 721 544 289 72 3 MspA1I CmG'CkG Cuts at: 0 32 43 57 144 766 820 1518 1613 Size: 32 11 14 87 622 54 698 95 Cuts at: 1613 1629 Size: 16 Fragments arranged by size: 698 622 95 87 54 32 16 14 11 NarI GG'CG_CC Cuts at: 0 1016 1172 1629 Size: 1016 156 457 Fragments arranged by size: 1016 457 156 PflMI CCAn_nnn'nTGG Cuts at: 0 108 1437 1629 Size: 108 1329 192 Fragments arranged by size: 1329 192 108 PfoI T'CCnGG_A Cuts at: 0 212 302 431 731 1271 1577 1629 Size: 212 90 129 300 540 306 52 Fragments arranged by size: 540 306 300 212 129 90 52 PmlI CAC'GTG Cuts at: 0 624 1629 Size: 624 1005 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1189 1221 1629 Size: 1189 32 408 Fragments arranged by size: 1189 408 32 PpuMI rG'GwC_Cy Cuts at: 0 503 550 570 1125 1629 Size: 503 47 20 555 504 Fragments arranged by size: 555 504 503 47 20 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1492 1524 1629 Size: 1492 32 105 Fragments arranged by size: 1492 105 32 PstI C_TGCA'G Cuts at: 0 374 513 850 1397 1517 1629 Size: 374 139 337 547 120 112 Fragments arranged by size: 547 374 337 139 120 112 PvuII CAG'CTG Cuts at: 0 144 1518 1629 Size: 144 1374 111 Fragments arranged by size: 1374 144 111 SacII CC_GC'GG Cuts at: 0 33 44 1629 Size: 33 11 1585 Fragments arranged by size: 1585 33 11 SexAI A'CCwGG_T Cuts at: 0 572 1629 Size: 572 1057 SfcI C'TryA_G Cuts at: 0 370 509 846 1393 1513 1629 Size: 370 139 337 547 120 116 Fragments arranged by size: 547 370 337 139 120 116 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1138 1629 Size: 1138 491 SfoI GGC'GCC Cuts at: 0 1017 1173 1629 Size: 1017 156 456 Fragments arranged by size: 1017 456 156 SmaI CCC'GGG Cuts at: 0 295 423 1629 Size: 295 128 1206 Fragments arranged by size: 1206 295 128 StuI AGG'CCT Cuts at: 0 977 1586 1629 Size: 977 609 43 Fragments arranged by size: 977 609 43 StyI C'CwwG_G Cuts at: 0 362 893 1629 Size: 362 531 736 Fragments arranged by size: 736 531 362 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 70 254 822 1629 Size: 70 184 568 807 Fragments arranged by size: 807 568 184 70 TatI w'GTAC_w Cuts at: 0 656 989 1629 Size: 656 333 640 Fragments arranged by size: 656 640 333 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 174 1125 1279 1629 Size: 174 951 154 350 Fragments arranged by size: 951 350 174 154 XmaI C'CCGG_G Cuts at: 0 293 421 1629 Size: 293 128 1208 Fragments arranged by size: 1208 293 128 XmnI GAAnn'nnTTC Cuts at: 0 151 1327 1629 Size: 151 1176 302 Fragments arranged by size: 1176 302 151 Enzymes that do cut: AarI AccI AcuI AflIII AloI AlwNI AvaI BanI BanII BbeI BbsI BcgI BglI Bme1580I BplI BpmI Bpu10I BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BstYI BtgI BtgZI BtsI DraIII EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NarI PflMI PfoI PmlI PpiI PpuMI PsrI PstI PvuII SacII SexAI SfcI SfiI SfoI SmaI StuI StyI TaqII TatI XcmI XmaI XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BciVI BclI BfrBI BglII BlpI BmgBI BmrI BmtI BpuEI BsaI BsaWI BsgI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraI DrdI EcoICRI EcoRI EcoRV FseI FspI FspAI HindIII HpaI KpnI MfeI MluI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PshAI PsiI PspOMI PspXI PvuI RsrII SacI SalI SanDI SapI SbfI ScaI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I XbaI XhoI ZraI