(Circular) (Six-base) MAPSORT of: pFN21AE4082.seq Check: 8678 from: 1 to: 5992 pFN21AE4082 5992 bp With 182 enzymes: SgfI * January 30, 2014 17:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2879 3802 278 Size: 53 83 186 936 1343 923 2468 Fragments arranged by size: 2468 1343 936 923 186 83 53 AccI GT'mk_AC Cuts at: 1937 2601 2879 3251 3335 1937 Size: 664 278 372 84 4594 Fragments arranged by size: 4594 664 372 278 84 Acc65I G'GTAC_C Cuts at: 3313 3313 Size: 5992 AclI AA'CG_TT Cuts at: 1563 4119 4492 1563 Size: 2556 373 3063 Fragments arranged by size: 3063 2556 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3094 4055 5439 5707 1632 Size: 1462 961 1384 268 1917 Fragments arranged by size: 1917 1462 1384 961 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5795 AflIII A'CryG_T Cuts at: 1203 2424 4891 5868 1203 Size: 1221 2467 977 1327 Fragments arranged by size: 2467 1327 1221 977 AgeI A'CCGG_T Cuts at: 4823 4823 Size: 5992 AhdI GACnn_n'nnGTC Cuts at: 4721 4721 Size: 5992 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5992 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5992 AlwNI CAG_nnn'CTG Cuts at: 2668 3735 5307 2668 Size: 1067 1572 3353 Fragments arranged by size: 3353 1572 1067 ApaI G_GGCC'C Cuts at: 2842 2842 Size: 5992 ApaLI G'TGCA_C Cuts at: 2558 4049 5205 2558 Size: 1491 1156 3345 Fragments arranged by size: 3345 1491 1156 ApoI r'AATT_y Cuts at: 1493 3301 3527 3679 5788 1493 Size: 1808 226 152 2109 1697 Fragments arranged by size: 2109 1808 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4546 160 Size: 872 3514 1606 Fragments arranged by size: 3514 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5992 AvaI C'yCGr_G Cuts at: 1870 1943 2326 2381 3317 1870 Size: 73 383 55 936 4545 Fragments arranged by size: 4545 936 383 73 55 BamHI G'GATC_C Cuts at: 3322 3689 3322 Size: 367 5625 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2284 3313 4768 618 Size: 325 217 627 497 1029 1455 1842 Fragments arranged by size: 1842 1455 1029 627 497 325 217 BanII G_rGCy'C Cuts at: 1391 2242 2333 2474 2842 3311 1391 Size: 851 91 141 368 469 4072 Fragments arranged by size: 4072 851 469 368 141 91 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2325 2879 3087 928 Size: 971 426 554 208 3833 Fragments arranged by size: 3833 971 554 426 208 BbvCI CC'TCA_GC Cuts at: 3265 3265 Size: 5992 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2912 2946 4183 4217 1903 Size: 34 50 34 891 34 1237 34 3678 Fragments arranged by size: 3678 1237 891 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3884 5094 3884 Size: 1210 4782 BclI T'GATC_A Cuts at: 1548 1548 Size: 5992 BfrBI ATG'CAT Cuts at: 3009 3009 Size: 5992 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2246 4603 136 Size: 107 122 71 631 605 574 2357 1525 Fragments arranged by size: 2357 1525 631 605 574 122 107 71 BglII A'GATC_T Cuts at: 5987 5987 Size: 5992 BlpI GC'TnA_GC Cuts at: 3409 3409 Size: 5992 Bme1580I G_kGCm'C Cuts at: 1790 2289 2562 2842 4053 5209 1790 Size: 499 273 280 1211 1156 2573 Fragments arranged by size: 2573 1211 1156 499 280 273 BmgBI CAC'GTC Cuts at: 1318 2599 3234 1318 Size: 1281 635 4076 Fragments arranged by size: 4076 1281 635 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2048 2462 3723 Size: 416 53 83 399 24 631 414 1261 Cuts at: 3723 4681 442 Size: 958 1753 Fragments arranged by size: 1753 1261 958 631 416 414 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5992 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2674 3142 2674 Size: 468 5524 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2157 2264 3209 3260 4652 1239 Size: 918 107 945 51 1392 2579 Fragments arranged by size: 2579 1392 945 918 107 51 Bpu10I CC'TnA_GC Cuts at: 3265 3265 Size: 5992 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2498 2632 2954 4117 4982 5280 2498 Size: 134 322 1163 865 298 3210 Fragments arranged by size: 3210 1163 865 322 298 134 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2128 4655 882 Size: 617 629 2527 2219 Fragments arranged by size: 2527 2219 629 617 BsaAI yAC'GTr Cuts at: 493 1204 2913 3783 493 Size: 711 1709 870 2702 Fragments arranged by size: 2702 1709 870 711 BsaBI GATnn'nnATC Cuts at: 2627 5986 2627 Size: 3359 2633 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2367 2876 3799 4181 Size: 53 83 186 936 834 509 923 382 Cuts at: 4181 4816 275 Size: 635 1451 Fragments arranged by size: 1451 936 923 834 635 509 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2020 4425 4823 5097 5244 5655 2020 Size: 2405 398 274 147 411 2357 Fragments arranged by size: 2405 2357 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2555 2585 3233 3263 1178 Size: 30 165 30 1152 30 648 30 3907 Fragments arranged by size: 3907 1152 648 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2969 3253 1186 Size: 1783 284 3925 Fragments arranged by size: 3925 1783 284 BseYI C'CCAG_C Cuts at: 2529 2776 3196 5195 5691 2529 Size: 247 420 1999 496 2830 Fragments arranged by size: 2830 1999 496 420 247 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2485 1163 Size: 573 749 4670 Fragments arranged by size: 4670 749 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2231 2867 3442 4203 5231 1540 Size: 275 186 230 636 575 761 1028 2301 Fragments arranged by size: 2301 1028 761 636 575 275 230 186 BsiHKAI G_wGCw'C Cuts at: 2028 2333 2562 4053 4138 5209 2028 Size: 305 229 1491 85 1071 2811 Fragments arranged by size: 2811 1491 1071 305 229 85 BsmI GAATG_Cn' Cuts at: 3503 3596 3503 Size: 93 5899 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2028 2242 2289 2333 2474 2562 2842 Size: 399 238 214 47 44 141 88 280 Cuts at: 2842 3311 4053 4138 5209 1391 Size: 469 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 469 399 280 238 214 141 88 85 47 44 BspEI T'CCGG_A Cuts at: 2020 5655 2020 Size: 3635 2357 BspHI T'CATG_A Cuts at: 3881 5611 3881 Size: 1730 4262 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2676 2891 3347 844 Size: 1832 215 456 3489 Fragments arranged by size: 3489 1832 456 215 BsrBI CCG'CTC Cuts at: 1394 1823 2011 2463 3879 1394 Size: 429 188 452 1416 3507 Fragments arranged by size: 3507 1416 452 429 188 BsrDI GCAATG_nn' Cuts at: 65 1248 2475 4487 4661 65 Size: 1183 1227 2012 174 1396 Fragments arranged by size: 2012 1396 1227 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2353 2537 2866 3141 4636 4823 1700 Size: 110 543 184 329 275 1495 187 2869 Fragments arranged by size: 2869 1495 543 329 275 187 184 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5992 BssHII G'CGCG_C Cuts at: 1925 2533 1925 Size: 608 5384 BssSI C'ACGA_G Cuts at: 2243 2582 4052 5064 2243 Size: 339 1470 1012 3171 Fragments arranged by size: 3171 1470 1012 339 BstAPI GCAn_nnn'nTGC Cuts at: 794 2032 2668 794 Size: 1238 636 4118 Fragments arranged by size: 4118 1238 636 BstBI TT'CG_AA Cuts at: 4796 4796 Size: 5992 BstEII G'GTnAC_C Cuts at: 1184 2209 1184 Size: 1025 4967 BstXI CCAn_nnnn'nTGG Cuts at: 2941 3238 2941 Size: 297 5695 BstYI r'GATC_y Cuts at: 1972 2347 2973 3322 3689 4074 4091 5543 5629 Size: 375 626 349 367 385 17 1452 86 Cuts at: 5629 5641 5987 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 626 385 375 367 349 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3252 3252 Size: 5992 Bsu36I CC'TnA_GG Cuts at: 3276 3276 Size: 5992 BtgI C'CryG_G Cuts at: 513 1065 2214 2225 513 Size: 552 1149 11 4280 Fragments arranged by size: 4280 1149 552 11 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5253 Fragments arranged by size: 5253 683 56 BtsI GCAGTG_nn' Cuts at: 2066 2304 2998 3214 3220 3508 4302 4322 2066 Size: 238 694 216 6 288 794 20 3736 Fragments arranged by size: 3736 794 694 288 238 216 20 6 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5992 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5957 DraI TTT'AAA Cuts at: 3296 3296 Size: 5992 DraIII CAC_nnn'GTG Cuts at: 2755 2755 Size: 5992 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2672 4999 809 Size: 507 1356 2327 1802 Fragments arranged by size: 2327 1802 1356 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2058 2116 2222 Size: 54 1425 50 275 18 228 58 106 Cuts at: 2222 2228 2550 2864 3069 3439 4328 5957 8 Size: 6 322 314 205 370 889 1629 43 Fragments arranged by size: 1629 1425 889 370 322 314 275 228 205 106 58 54 50 43 18 6 EagI C'GGCC_G Cuts at: 1537 1812 2228 2864 3439 1537 Size: 275 416 636 575 4090 Fragments arranged by size: 4090 636 575 416 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3922 1353 Size: 2569 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2477 4579 4953 5099 387 Size: 1064 138 888 2102 374 146 1280 Fragments arranged by size: 2102 1280 1064 888 374 146 138 EcoICRI GAG'CTC Cuts at: 2331 2331 Size: 5992 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2157 2264 3094 3209 3260 4055 4652 Size: 393 525 107 830 115 51 795 597 Cuts at: 4652 5439 5707 1239 Size: 787 268 1524 Fragments arranged by size: 1524 830 795 787 597 525 393 268 115 107 51 EcoNI CCTnn'n_nnAGG Cuts at: 2405 2405 Size: 5992 EcoO109I rG'GnC_Cy Cuts at: 2050 2124 2180 2443 2665 3121 3280 2050 Size: 74 56 263 222 456 159 4762 Fragments arranged by size: 4762 456 263 222 159 74 56 EcoRI G'AATT_C Cuts at: 3301 3679 3301 Size: 378 5614 FspI TGC'GCA Cuts at: 4498 4498 Size: 5992 HaeII r_GCGC'y Cuts at: 1678 2360 3043 5139 1678 Size: 682 683 2096 2531 Fragments arranged by size: 2531 2096 683 682 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4633 4665 4707 4739 1373 Size: 32 135 32 3061 32 42 32 2626 Fragments arranged by size: 3061 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3336 3582 669 Size: 988 281 1398 246 3079 Fragments arranged by size: 3079 1398 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5992 HpaI GTT'AAC Cuts at: 3582 3582 Size: 5992 KpnI G_GTAC'C Cuts at: 3317 3317 Size: 5992 MfeI C'AATT_G Cuts at: 3591 3591 Size: 5992 MluI A'CGCG_T Cuts at: 2424 5868 2424 Size: 3444 2548 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2117 2598 5106 5290 1305 Size: 812 481 2508 184 2007 Fragments arranged by size: 2508 2007 812 481 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2118 2224 10 Size: 54 1425 343 286 106 3778 Fragments arranged by size: 3778 1425 343 286 106 54 MslI CAynn'nnrTG Cuts at: 518 1166 3950 4309 4468 518 Size: 648 2784 359 159 2042 Fragments arranged by size: 2784 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3408 4085 5233 5478 1606 Size: 1802 677 1148 245 2120 Fragments arranged by size: 2120 1802 1148 677 245 NaeI GCC'GGC Cuts at: 2355 2539 2355 Size: 184 5808 NcoI C'CATG_G Cuts at: 513 1065 2214 513 Size: 552 1149 4291 Fragments arranged by size: 4291 1149 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5992 NgoMIV G'CCGG_C Cuts at: 2353 2537 2353 Size: 184 5808 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5992 NotI GC'GGCC_GC Cuts at: 3439 3439 Size: 5992 NsiI A_TGCA'T Cuts at: 3011 3011 Size: 5992 NspI r_CATG'y Cuts at: 1132 1229 3350 4895 1132 Size: 97 2121 1545 2229 Fragments arranged by size: 2229 2121 1545 97 PasI CC'CwG_GG Cuts at: 1348 2129 1348 Size: 781 5211 PciI A'CATG_T Cuts at: 4891 4891 Size: 5992 PflMI CCAn_nnn'nTGG Cuts at: 1108 2220 2740 1108 Size: 1112 520 4360 Fragments arranged by size: 4360 1112 520 PfoI T'CCnGG_A Cuts at: 3179 3179 Size: 5992 PmeI GTTT'AAAC Cuts at: 3296 3296 Size: 5992 PmlI CAC'GTG Cuts at: 2913 2913 Size: 5992 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4061 4093 5574 5606 4061 Size: 32 1481 32 4447 Fragments arranged by size: 4447 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2050 2180 2665 3121 2050 Size: 130 485 456 4921 Fragments arranged by size: 4921 485 456 130 PshAI GACnn'nnGTC Cuts at: 1613 2597 1613 Size: 984 5008 PsiI TTA'TAA Cuts at: 3562 3562 Size: 5992 PspOMI G'GGCC_C Cuts at: 2838 2838 Size: 5992 PspXI vC'TCGA_Gb Cuts at: 2326 2326 Size: 5992 PstI C_TGCA'G Cuts at: 830 2855 3227 3344 830 Size: 2025 372 117 3478 Fragments arranged by size: 3478 2025 372 117 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5992 SacI G_AGCT'C Cuts at: 2333 2333 Size: 5992 SalI G'TCGA_C Cuts at: 1936 3334 1936 Size: 1398 4594 SanDI GG'GwC_CC Cuts at: 2050 2050 Size: 5992 SbfI CC_TGCA'GG Cuts at: 3344 3344 Size: 5992 ScaI AGT'ACT Cuts at: 4240 5952 4240 Size: 1712 4280 SfcI C'TryA_G Cuts at: 826 1045 2851 3103 3223 3340 4475 5156 5347 Size: 219 1806 252 120 117 1135 681 191 Cuts at: 5347 826 Size: 1471 Fragments arranged by size: 1806 1471 1135 681 252 219 191 120 117 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5992 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5992 SmaI CCC'GGG Cuts at: 1872 3319 1872 Size: 1447 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2326 2513 2611 2969 4096 4997 Size: 197 926 383 187 98 358 1127 901 Cuts at: 4997 5259 820 Size: 262 1553 Fragments arranged by size: 1553 1127 926 901 383 358 262 197 187 98 SnaBI TAC'GTA Cuts at: 493 3783 493 Size: 3290 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5992 SphI G_CATG'C Cuts at: 1132 3350 1132 Size: 2218 3774 SspI AAT'ATT Cuts at: 5 52 3916 5 Size: 47 3864 2081 Fragments arranged by size: 3864 2081 47 StuI AGG'CCT Cuts at: 1517 2156 2846 1517 Size: 639 690 4663 Fragments arranged by size: 4663 690 639 StyI C'CwwG_G Cuts at: 513 1065 2214 3431 513 Size: 552 1149 1217 3074 Fragments arranged by size: 3074 1217 1149 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2761 4019 4036 4189 4374 4819 325 Size: 1255 1181 1258 17 153 185 445 1498 Fragments arranged by size: 1498 1258 1255 1181 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4238 5950 96 Size: 274 80 33 51 1443 2261 1712 138 Fragments arranged by size: 2261 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5952 5984 5952 Size: 32 5960 Tth111I GACn'n_nGTC Cuts at: 1316 2764 1316 Size: 1448 4544 XbaI T'CTAG_A Cuts at: 3328 3328 Size: 5992 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2126 2126 Size: 5992 XhoI C'TCGA_G Cuts at: 1943 2326 1943 Size: 383 5609 XmaI C'CCGG_G Cuts at: 1870 3317 1870 Size: 1447 4545 XmnI GAAnn'nnTTC Cuts at: 4121 4121 Size: 5992 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2877 3800 276 Size: 53 83 186 936 1343 923 2468 Fragments arranged by size: 2468 1343 936 923 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BsiWI BsmBI EcoRV FalI FseI FspAI KasI NarI NruI PacI PsrI PvuII RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI