(Linear) (Six-base) MAPSORT of: KIEE1908.seq Check: 8254 from: 1 to: 1245 KIEE1908.orf With 182 enzymes: SgfI * July 15, 2010 18:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 166 1245 Size: 166 1079 AgeI A'CCGG_T Cuts at: 0 1136 1245 Size: 1136 109 AleI CACnn'nnGTG Cuts at: 0 949 1245 Size: 949 296 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 460 492 1245 Size: 460 32 753 Fragments arranged by size: 753 460 32 ApaI G_GGCC'C Cuts at: 0 823 1245 Size: 823 422 ApaLI G'TGCA_C Cuts at: 0 340 1245 Size: 340 905 AvaI C'yCGr_G Cuts at: 0 532 1000 1019 1166 1245 Size: 532 468 19 147 79 Fragments arranged by size: 532 468 147 79 19 BanI G'GyrC_C Cuts at: 0 8 154 443 1133 1245 Size: 8 146 289 690 112 Fragments arranged by size: 690 289 146 112 8 BanII G_rGCy'C Cuts at: 0 532 823 1245 Size: 532 291 422 Fragments arranged by size: 532 422 291 BbsI GAAGACnn'nnnn_ Cuts at: 0 1020 1245 Size: 1020 225 BclI T'GATC_A Cuts at: 0 842 1245 Size: 842 403 BglI GCCn_nnn'nGGC Cuts at: 0 1171 1245 Size: 1171 74 Bme1580I G_kGCm'C Cuts at: 0 11 344 716 823 1167 1245 Size: 11 333 372 107 344 78 Fragments arranged by size: 372 344 333 107 78 11 BmgBI CAC'GTC Cuts at: 0 908 1245 Size: 908 337 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 58 1245 Size: 58 1187 Bpu10I CC'TnA_GC Cuts at: 0 430 1245 Size: 430 815 BsaI GGTCTCn'nnnn_ Cuts at: 0 907 1020 1245 Size: 907 113 225 Fragments arranged by size: 907 225 113 BsaBI GATnn'nnATC Cuts at: 0 691 1245 Size: 691 554 BsaWI w'CCGG_w Cuts at: 0 440 1121 1136 1245 Size: 440 681 15 109 Fragments arranged by size: 681 440 109 15 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 370 400 460 490 1027 1057 1245 Size: 370 30 60 30 537 30 188 Fragments arranged by size: 537 370 188 60 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 337 1245 Size: 337 908 BseYI C'CCAG_C Cuts at: 0 275 704 1245 Size: 275 429 541 Fragments arranged by size: 541 429 275 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 582 622 1245 Size: 582 40 623 Fragments arranged by size: 623 582 40 BsiHKAI G_wGCw'C Cuts at: 0 344 656 1245 Size: 344 312 589 Fragments arranged by size: 589 344 312 BsmBI CGTCTCn'nnnn_ Cuts at: 0 28 66 1245 Size: 28 38 1179 Fragments arranged by size: 1179 38 28 Bsp1286I G_dGCh'C Cuts at: 0 11 344 532 656 716 823 1167 1245 Size: 11 333 188 124 60 107 344 78 Fragments arranged by size: 344 333 188 124 107 78 60 11 BspEI T'CCGG_A Cuts at: 0 1121 1245 Size: 1121 124 BspHI T'CATG_A Cuts at: 0 881 1245 Size: 881 364 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 166 554 644 1245 Size: 166 388 90 601 Fragments arranged by size: 601 388 166 90 BsrFI r'CCGG_y Cuts at: 0 671 1136 1245 Size: 671 465 109 Fragments arranged by size: 671 465 109 BsrGI T'GTAC_A Cuts at: 0 797 1245 Size: 797 448 BstAPI GCAn_nnn'nTGC Cuts at: 0 443 1245 Size: 443 802 BstXI CCAn_nnnn'nTGG Cuts at: 0 729 831 1245 Size: 729 102 414 Fragments arranged by size: 729 414 102 BstYI r'GATC_y Cuts at: 0 311 1245 Size: 311 934 BtgI C'CryG_G Cuts at: 0 140 1245 Size: 140 1105 BtsI GCAGTG_nn' Cuts at: 0 84 133 1245 Size: 84 49 1112 Fragments arranged by size: 1112 84 49 EaeI y'GGCC_r Cuts at: 0 104 263 1245 Size: 104 159 982 Fragments arranged by size: 982 159 104 EarI CTCTTCn'nnn_ Cuts at: 0 1120 1245 Size: 1120 125 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 468 1245 Size: 468 777 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 58 1245 Size: 58 1187 EcoNI CCTnn'n_nnAGG Cuts at: 0 319 1245 Size: 319 926 EcoO109I rG'GnC_Cy Cuts at: 0 33 819 820 1245 Size: 33 786 1 425 Fragments arranged by size: 786 425 33 1 FspI TGC'GCA Cuts at: 0 493 679 1245 Size: 493 186 566 Fragments arranged by size: 566 493 186 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 82 114 1000 1032 1138 1170 1245 Size: 82 32 886 32 106 32 75 Fragments arranged by size: 886 106 82 75 32 32 32 MscI TGG'CCA Cuts at: 0 106 265 1245 Size: 106 159 980 Fragments arranged by size: 980 159 106 MslI CAynn'nnrTG Cuts at: 0 347 727 880 949 979 1245 Size: 347 380 153 69 30 266 Fragments arranged by size: 380 347 266 153 69 30 MspA1I CmG'CkG Cuts at: 0 275 1245 Size: 275 970 NspI r_CATG'y Cuts at: 0 984 1245 Size: 984 261 PfoI T'CCnGG_A Cuts at: 0 74 1049 1245 Size: 74 975 196 Fragments arranged by size: 975 196 74 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 46 78 460 492 1245 Size: 46 32 382 32 753 Fragments arranged by size: 753 382 46 32 32 PspOMI G'GGCC_C Cuts at: 0 819 1245 Size: 819 426 PstI C_TGCA'G Cuts at: 0 128 1245 Size: 128 1117 PvuII CAG'CTG Cuts at: 0 275 1245 Size: 275 970 SexAI A'CCwGG_T Cuts at: 0 502 1245 Size: 502 743 SfcI C'TryA_G Cuts at: 0 124 1245 Size: 124 1121 SgrAI Cr'CCGG_yG Cuts at: 0 1136 1245 Size: 1136 109 SmaI CCC'GGG Cuts at: 0 1002 1021 1245 Size: 1002 19 224 Fragments arranged by size: 1002 224 19 StuI AGG'CCT Cuts at: 0 789 1245 Size: 789 456 TatI w'GTAC_w Cuts at: 0 752 797 923 1245 Size: 752 45 126 322 Fragments arranged by size: 752 322 126 45 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 370 402 1245 Size: 370 32 843 Fragments arranged by size: 843 370 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 507 1245 Size: 507 738 XmaI C'CCGG_G Cuts at: 0 1000 1019 1245 Size: 1000 19 226 Fragments arranged by size: 1000 226 19 Enzymes that do cut: AarI AgeI AleI AloI ApaI ApaLI AvaI BanI BanII BbsI BclI BglI Bme1580I BmgBI BpmI Bpu10I BsaI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrFI BsrGI BstAPI BstXI BstYI BtgI BtsI EaeI EarI EciI Eco57MI EcoNI EcoO109I FspI Hin4I MscI MslI MspA1I NspI PfoI PpiI PspOMI PstI PvuII SexAI SfcI SgrAI SmaI StuI TatI TstI XcmI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AhdI AlfI AlwNI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BfrBI BglII BlpI BmrI BmtI BplI BpuEI BsaAI BsaHI BsiEI BsiWI BsmI BsrBI BsrDI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoRI EcoRV FalI FseI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PflMI PmeI PmlI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI SmlI SnaBI SpeI SphI SrfI SspI StyI SwaI TaqII Tth111I XbaI XhoI XmnI ZraI