(Circular) (Six-base) MAPSORT of: pFN21AE2242.seq Check: 2222 from: 1 to: 5746 pFN21AE2242 5746 bp With 182 enzymes: SgfI * July 6, 2012 17:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3556 278 Size: 53 83 186 936 2020 2468 Fragments arranged by size: 2468 2020 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3089 1937 Size: 1152 4594 Acc65I G'GTAC_C Cuts at: 2900 3067 2900 Size: 167 5579 AclI AA'CG_TT Cuts at: 1563 3045 3873 4246 1563 Size: 1482 828 373 3063 Fragments arranged by size: 3063 1482 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2804 3809 5193 5461 1632 Size: 1172 1005 1384 268 1917 Fragments arranged by size: 1917 1384 1172 1005 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5549 AflIII A'CryG_T Cuts at: 1203 4645 5622 1203 Size: 3442 977 1327 Fragments arranged by size: 3442 1327 977 AgeI A'CCGG_T Cuts at: 4577 4577 Size: 5746 AhdI GACnn_n'nnGTC Cuts at: 4475 4475 Size: 5746 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5746 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5746 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2474 2506 2474 Size: 32 5714 AlwNI CAG_nnn'CTG Cuts at: 2806 3489 5061 2806 Size: 683 1572 3491 Fragments arranged by size: 3491 1572 683 ApaI G_GGCC'C Cuts at: 2736 2736 Size: 5746 ApaLI G'TGCA_C Cuts at: 2662 3803 4959 2662 Size: 1141 1156 3449 Fragments arranged by size: 3449 1156 1141 ApoI r'AATT_y Cuts at: 1493 3055 3281 3433 5542 1493 Size: 1562 226 152 2109 1697 Fragments arranged by size: 2109 1697 1562 226 152 AseI AT'TA_AT Cuts at: 160 1032 4300 160 Size: 872 3268 1606 Fragments arranged by size: 3268 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5746 AvaI C'yCGr_G Cuts at: 1870 1943 2729 3071 1870 Size: 73 786 342 4545 Fragments arranged by size: 4545 786 342 73 AvrII C'CTAG_G Cuts at: 2974 2974 Size: 5746 BamHI G'GATC_C Cuts at: 2545 3076 3443 2545 Size: 531 367 4848 Fragments arranged by size: 4848 531 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2900 3010 3067 4522 618 Size: 325 217 627 1113 110 57 1455 1842 Fragments arranged by size: 1842 1455 1113 627 325 217 110 57 BanII G_rGCy'C Cuts at: 1391 2736 2765 3065 1391 Size: 1345 29 300 4072 Fragments arranged by size: 4072 1345 300 29 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4775 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2329 2363 3937 3971 1903 Size: 34 392 34 1574 34 3678 Fragments arranged by size: 3678 1574 392 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3638 4848 3638 Size: 1210 4536 BclI T'GATC_A Cuts at: 1548 2808 1548 Size: 1260 4486 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4357 136 Size: 107 122 71 631 605 2685 1525 Fragments arranged by size: 2685 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5741 5741 Size: 5746 BlpI GC'TnA_GC Cuts at: 3163 3163 Size: 5746 Bme1580I G_kGCm'C Cuts at: 1790 2666 2736 3807 4963 1790 Size: 876 70 1071 1156 2573 Fragments arranged by size: 2573 1156 1071 876 70 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5746 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2580 3477 4435 Size: 416 53 83 399 24 1163 897 958 Cuts at: 4435 442 Size: 1753 Fragments arranged by size: 1753 1163 958 897 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5746 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2330 4406 1239 Size: 1091 2076 2579 Fragments arranged by size: 2579 2076 1091 Bpu10I CC'TnA_GC Cuts at: 2064 2064 Size: 5746 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3871 4736 5034 3871 Size: 865 298 4583 Fragments arranged by size: 4583 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2579 4409 882 Size: 617 1080 1830 2219 Fragments arranged by size: 2219 1830 1080 617 BsaAI yAC'GTr Cuts at: 493 1204 3537 493 Size: 711 2333 2702 Fragments arranged by size: 2702 2333 711 BsaBI GATnn'nnATC Cuts at: 5740 5740 Size: 5746 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3020 3553 3935 4570 Size: 53 83 186 936 1487 533 382 635 Cuts at: 4570 275 Size: 1451 Fragments arranged by size: 1487 1451 936 635 533 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4179 4577 4851 4998 5409 4179 Size: 398 274 147 411 4516 Fragments arranged by size: 4516 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2006 2036 1178 Size: 30 165 30 603 30 4888 Fragments arranged by size: 4888 603 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2615 2824 2854 1186 Size: 1429 209 30 4078 Fragments arranged by size: 4078 1429 209 30 BseYI C'CCAG_C Cuts at: 3023 4949 5445 3023 Size: 1926 496 3324 Fragments arranged by size: 3324 1926 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5173 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3196 3957 4985 1540 Size: 275 186 1195 761 1028 2301 Fragments arranged by size: 2301 1195 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2666 2765 3807 3892 4963 2666 Size: 99 1042 85 1071 3449 Fragments arranged by size: 3449 1071 1042 99 85 BsiWI C'GTAC_G Cuts at: 2713 2713 Size: 5746 BsmI GAATG_Cn' Cuts at: 3257 3350 3257 Size: 93 5653 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2666 2736 2765 3065 3807 3892 4963 Size: 399 876 70 29 300 742 85 1071 Cuts at: 4963 1391 Size: 2174 Fragments arranged by size: 2174 1071 876 742 399 300 85 70 29 BspEI T'CCGG_A Cuts at: 5409 5409 Size: 5746 BspHI T'CATG_A Cuts at: 3635 5365 3635 Size: 1730 4016 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3101 844 Size: 2257 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3633 1394 Size: 429 1810 3507 Fragments arranged by size: 3507 1810 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2280 2633 4241 4415 65 Size: 1183 1032 353 1608 174 1396 Fragments arranged by size: 1608 1396 1183 1032 353 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2814 3007 4390 4577 1700 Size: 110 1004 193 1383 187 2869 Fragments arranged by size: 2869 1383 1004 193 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5746 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5746 BssSI C'ACGA_G Cuts at: 3806 4818 3806 Size: 1012 4734 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5746 BstBI TT'CG_AA Cuts at: 4550 4550 Size: 5746 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5746 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 5746 BstYI r'GATC_y Cuts at: 1972 2501 2545 3076 3443 3828 3845 5297 5383 Size: 529 44 531 367 385 17 1452 86 Cuts at: 5383 5395 5741 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 531 529 385 367 346 86 44 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5194 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2044 487 Size: 683 56 818 4189 Fragments arranged by size: 4189 818 683 56 BtsI GCAGTG_nn' Cuts at: 3262 4056 4076 3262 Size: 794 20 4932 Fragments arranged by size: 4932 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5746 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5711 DraI TTT'AAA Cuts at: 3050 3050 Size: 5746 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4753 809 Size: 507 3437 1802 Fragments arranged by size: 3437 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3193 4082 5711 Size: 54 1425 50 275 18 1363 889 1629 Cuts at: 5711 8 Size: 43 Fragments arranged by size: 1629 1425 1363 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3193 1537 Size: 275 1381 4090 Fragments arranged by size: 4090 1381 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2237 2459 3676 1353 Size: 884 222 1217 3423 Fragments arranged by size: 3423 1217 884 222 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4333 4707 4853 387 Size: 1064 138 2744 374 146 1280 Fragments arranged by size: 2744 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2763 2763 Size: 5746 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2330 2804 3809 4406 5193 5461 1239 Size: 393 698 474 1005 597 787 268 1524 Fragments arranged by size: 1524 1005 787 698 597 474 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2087 2750 2087 Size: 663 5083 EcoO109I rG'GnC_Cy Cuts at: 2670 2733 2803 2670 Size: 63 70 5613 Fragments arranged by size: 5613 70 63 EcoRI G'AATT_C Cuts at: 3055 3433 3055 Size: 378 5368 FspI TGC'GCA Cuts at: 4252 4252 Size: 5746 HaeII r_GCGC'y Cuts at: 1678 4893 1678 Size: 3215 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2006 2038 2819 2846 2851 Size: 32 135 32 434 32 781 27 5 Cuts at: 2851 2866 2878 2898 4387 4419 4461 4493 1373 Size: 15 12 20 1489 32 42 32 2626 Fragments arranged by size: 2626 1489 781 434 135 42 32 32 32 32 32 27 20 15 12 5 HincII GTy'rAC Cuts at: 669 1657 1938 3090 3336 669 Size: 988 281 1152 246 3079 Fragments arranged by size: 3079 1152 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5746 HpaI GTT'AAC Cuts at: 3336 3336 Size: 5746 KpnI G_GTAC'C Cuts at: 2904 3071 2904 Size: 167 5579 MfeI C'AATT_G Cuts at: 2266 3345 2266 Size: 1079 4667 MluI A'CGCG_T Cuts at: 5622 5622 Size: 5746 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2312 3006 4860 5044 1305 Size: 1007 694 1854 184 2007 Fragments arranged by size: 2007 1854 1007 694 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3924 Fragments arranged by size: 3924 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2523 2694 3704 4063 4222 518 Size: 648 843 514 171 1010 359 159 2042 Fragments arranged by size: 2042 1010 843 648 514 359 171 159 MspA1I CmG'CkG Cuts at: 1606 3162 3839 4987 5232 1606 Size: 1556 677 1148 245 2120 Fragments arranged by size: 2120 1556 1148 677 245 NaeI GCC'GGC Cuts at: 2816 2816 Size: 5746 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5194 NdeI CA'TA_TG Cuts at: 387 387 Size: 5746 NgoMIV G'CCGG_C Cuts at: 2814 2814 Size: 5746 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5746 NotI GC'GGCC_GC Cuts at: 3193 3193 Size: 5746 NspI r_CATG'y Cuts at: 1132 1229 3104 4649 1132 Size: 97 1875 1545 2229 Fragments arranged by size: 2229 1875 1545 97 PasI CC'CwG_GG Cuts at: 1348 2590 1348 Size: 1242 4504 PciI A'CATG_T Cuts at: 4645 4645 Size: 5746 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5746 PmeI GTTT'AAAC Cuts at: 3050 3050 Size: 5746 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3815 3847 5328 5360 3815 Size: 32 1481 32 4201 Fragments arranged by size: 4201 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2670 2670 Size: 5746 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5746 PsiI TTA'TAA Cuts at: 3316 3316 Size: 5746 PspOMI G'GGCC_C Cuts at: 2732 2732 Size: 5746 PstI C_TGCA'G Cuts at: 830 3098 830 Size: 2268 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5746 SacI G_AGCT'C Cuts at: 2765 2765 Size: 5746 SalI G'TCGA_C Cuts at: 1936 3088 1936 Size: 1152 4594 SapI GCTCTTCn'nnn_ Cuts at: 2237 2237 Size: 5746 SbfI CC_TGCA'GG Cuts at: 3098 3098 Size: 5746 ScaI AGT'ACT Cuts at: 3994 5706 3994 Size: 1712 4034 SexAI A'CCwGG_T Cuts at: 2082 2082 Size: 5746 SfcI C'TryA_G Cuts at: 826 1045 3094 4229 4910 5101 826 Size: 219 2049 1135 681 191 1471 Fragments arranged by size: 2049 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5746 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5746 SmaI CCC'GGG Cuts at: 1872 3073 1872 Size: 1201 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3850 4751 5013 820 Size: 197 926 1907 901 262 1553 Fragments arranged by size: 1907 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3537 493 Size: 3044 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5746 SphI G_CATG'C Cuts at: 1132 3104 1132 Size: 1972 3774 SspI AAT'ATT Cuts at: 5 52 3670 5 Size: 47 3618 2081 Fragments arranged by size: 3618 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5746 StyI C'CwwG_G Cuts at: 513 1065 2974 3185 513 Size: 552 1909 211 3074 Fragments arranged by size: 3074 1909 552 211 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3773 3790 3943 4128 4573 325 Size: 1255 2193 17 153 185 445 1498 Fragments arranged by size: 2193 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3992 5704 96 Size: 274 80 33 51 1443 2015 1712 138 Fragments arranged by size: 2015 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5706 5738 5706 Size: 32 5714 Tth111I GACn'n_nGTC Cuts at: 1316 2084 1316 Size: 768 4978 XbaI T'CTAG_A Cuts at: 3082 3082 Size: 5746 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5746 XmaI C'CCGG_G Cuts at: 1870 3071 1870 Size: 1201 4545 XmnI GAAnn'nnTTC Cuts at: 3875 3875 Size: 5746 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3554 276 Size: 53 83 186 936 2020 2468 Fragments arranged by size: 2468 2020 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI BaeI BbeI BbvCI BfrBI BplI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacII SanDI SfiI SfoI SrfI SwaI XcmI