(Circular) (Six-base) MAPSORT of: pFN21AE9251.seq Check: 5251 from: 1 to: 6388 pFN21AE9251 6388 bp With 182 enzymes: SgfI * August 1, 2019 17:59 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3627 3627 Size: 6388 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4198 278 Size: 53 83 186 936 2662 2468 Fragments arranged by size: 2662 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2655 3731 1937 Size: 718 1076 4594 Fragments arranged by size: 4594 1076 718 Acc65I G'GTAC_C Cuts at: 3709 3709 Size: 6388 AclI AA'CG_TT Cuts at: 1563 4515 4888 1563 Size: 2952 373 3063 Fragments arranged by size: 3063 2952 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2294 2579 2823 3144 3276 4451 5835 6103 Size: 662 285 244 321 132 1175 1384 268 Cuts at: 6103 1632 Size: 1917 Fragments arranged by size: 1917 1384 1175 662 321 285 268 244 132 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6191 AflIII A'CryG_T Cuts at: 1203 2056 2763 5287 6264 1203 Size: 853 707 2524 977 1327 Fragments arranged by size: 2524 1327 977 853 707 AgeI A'CCGG_T Cuts at: 5219 5219 Size: 6388 AhdI GACnn_n'nnGTC Cuts at: 5117 5117 Size: 6388 AleI CACnn'nnGTG Cuts at: 1166 3101 1166 Size: 1935 4453 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2066 1184 Size: 882 5506 AlwNI CAG_nnn'CTG Cuts at: 2473 2512 2890 3272 3299 3574 4131 5703 2473 Size: 39 378 382 27 275 557 1572 3158 Fragments arranged by size: 3158 1572 557 382 378 275 39 27 ApaLI G'TGCA_C Cuts at: 4445 5601 4445 Size: 1156 5232 ApoI r'AATT_y Cuts at: 1493 3697 3923 4075 6184 1493 Size: 2204 226 152 2109 1697 Fragments arranged by size: 2204 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4942 160 Size: 872 3910 1606 Fragments arranged by size: 3910 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6388 AvaI C'yCGr_G Cuts at: 1870 1943 2106 2414 3713 1870 Size: 73 163 308 1299 4545 Fragments arranged by size: 4545 1299 308 163 73 AvrII C'CTAG_G Cuts at: 3329 3329 Size: 6388 BamHI G'GATC_C Cuts at: 3119 3718 4085 3119 Size: 599 367 5422 Fragments arranged by size: 5422 599 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2663 3615 3709 5164 618 Size: 325 217 627 876 952 94 1455 1842 Fragments arranged by size: 1842 1455 952 876 627 325 217 94 BanII G_rGCy'C Cuts at: 1391 2119 2128 2415 2421 2979 3707 1391 Size: 728 9 287 6 558 728 4072 Fragments arranged by size: 4072 728 728 558 287 9 6 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2811 3667 928 Size: 971 912 856 3649 Fragments arranged by size: 3649 971 912 856 BbvCI CC'TCA_GC Cuts at: 3087 3135 3087 Size: 48 6340 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4579 4613 1903 Size: 34 2642 34 3678 Fragments arranged by size: 3678 2642 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2059 4280 5490 2059 Size: 2221 1210 2957 Fragments arranged by size: 2957 2221 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6388 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4999 136 Size: 107 122 71 631 605 3327 1525 Fragments arranged by size: 3327 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6383 6383 Size: 6388 BlpI GC'TnA_GC Cuts at: 2811 3805 2811 Size: 994 5394 Bme1580I G_kGCm'C Cuts at: 1790 2244 4449 5605 1790 Size: 454 2205 1156 2573 Fragments arranged by size: 2573 2205 1156 454 BmgBI CAC'GTC Cuts at: 1318 2659 1318 Size: 1341 5047 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2805 2878 4119 Size: 416 53 83 399 24 1388 73 1241 Cuts at: 4119 5077 442 Size: 958 1753 Fragments arranged by size: 1753 1388 1241 958 416 399 83 73 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6388 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3193 3223 3456 3193 Size: 30 233 6125 Fragments arranged by size: 6125 233 30 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2133 2350 2414 2421 2622 2979 3193 3212 Size: 894 217 64 7 201 357 214 19 Cuts at: 3212 3518 3569 3647 5048 1239 Size: 306 51 78 1401 2579 Fragments arranged by size: 2579 1401 894 357 306 217 214 201 78 64 51 19 7 Bpu10I CC'TnA_GC Cuts at: 2409 2936 3087 3135 2409 Size: 527 151 48 5662 Fragments arranged by size: 5662 527 151 48 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2517 3275 3464 4513 5378 5676 2517 Size: 758 189 1049 865 298 3229 Fragments arranged by size: 3229 1049 865 758 298 189 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5051 882 Size: 617 3552 2219 Fragments arranged by size: 3552 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3601 4179 493 Size: 711 2397 578 2702 Fragments arranged by size: 2702 2397 711 578 BsaBI GATnn'nnATC Cuts at: 6382 6382 Size: 6388 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4195 4577 5212 275 Size: 53 83 186 936 2662 382 635 1451 Fragments arranged by size: 2662 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4821 5219 5493 5640 6051 4821 Size: 398 274 147 411 5158 Fragments arranged by size: 5158 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2015 2045 2419 2449 1178 Size: 30 165 30 612 30 374 30 5117 Fragments arranged by size: 5117 612 374 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2691 2921 1186 Size: 1505 230 4653 Fragments arranged by size: 4653 1505 230 BseYI C'CCAG_C Cuts at: 2727 3068 3265 5591 6087 2727 Size: 341 197 2326 496 3028 Fragments arranged by size: 3028 2326 496 341 197 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2630 2767 3581 1163 Size: 573 894 137 814 3970 Fragments arranged by size: 3970 894 814 573 137 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2827 3838 4599 5627 1540 Size: 275 186 826 1011 761 1028 2301 Fragments arranged by size: 2301 1028 1011 826 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2107 2415 2942 3061 4449 4534 5605 2107 Size: 308 527 119 1388 85 1071 2890 Fragments arranged by size: 2890 1388 1071 527 308 119 85 BsmI GAATG_Cn' Cuts at: 2545 3899 3992 2545 Size: 1354 93 4941 Fragments arranged by size: 4941 1354 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2107 2119 2128 2244 2415 2421 2942 Size: 399 317 12 9 116 171 6 521 Cuts at: 2942 2979 3061 3707 4449 4534 5605 1391 Size: 37 82 646 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 646 521 399 317 171 116 85 82 37 12 9 6 BspEI T'CCGG_A Cuts at: 6051 6051 Size: 6388 BspHI T'CATG_A Cuts at: 4277 6007 4277 Size: 1730 4658 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3627 3743 844 Size: 2783 116 3489 Fragments arranged by size: 3489 2783 116 BsrBI CCG'CTC Cuts at: 1394 1823 3613 4275 1394 Size: 429 1790 662 3507 Fragments arranged by size: 3507 1790 662 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4883 5057 65 Size: 1183 3635 174 1396 Fragments arranged by size: 3635 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2822 3344 3633 5032 5219 1700 Size: 110 1012 522 289 1399 187 2869 Fragments arranged by size: 2869 1399 1012 522 289 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6388 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6388 BssSI C'ACGA_G Cuts at: 4448 5460 4448 Size: 1012 5376 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6388 BstBI TT'CG_AA Cuts at: 5192 5192 Size: 6388 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6388 BstXI CCAn_nnnn'nTGG Cuts at: 2431 3377 2431 Size: 946 5442 BstYI r'GATC_y Cuts at: 1972 2564 2677 3119 3364 3718 4085 4470 4487 Size: 592 113 442 245 354 367 385 17 Cuts at: 4487 5939 6025 6037 6383 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 592 442 385 367 354 346 245 113 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2087 2194 2745 3426 513 Size: 552 939 83 107 551 681 3475 Fragments arranged by size: 3475 939 681 552 551 107 83 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5649 Fragments arranged by size: 5649 683 56 BtsI GCAGTG_nn' Cuts at: 2848 2953 3593 3904 4698 4718 2848 Size: 105 640 311 794 20 4518 Fragments arranged by size: 4518 794 640 311 105 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6388 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6353 DraI TTT'AAA Cuts at: 3692 3692 Size: 6388 DraIII CAC_nnn'GTG Cuts at: 3394 3394 Size: 6388 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5395 809 Size: 507 4079 1802 Fragments arranged by size: 4079 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2161 3520 3835 Size: 54 1425 50 275 18 331 1359 315 Cuts at: 3835 4724 6353 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1359 889 331 315 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3835 1537 Size: 275 2023 4090 Fragments arranged by size: 4090 2023 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2557 2598 2991 3075 4318 1353 Size: 1204 41 393 84 1243 3423 Fragments arranged by size: 3423 1243 1204 393 84 41 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4975 5349 5495 387 Size: 1064 138 3386 374 146 1280 Fragments arranged by size: 3386 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2413 2413 Size: 6388 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2133 2294 2350 2414 2421 2579 2622 Size: 393 501 161 56 64 7 158 43 Cuts at: 2622 2823 2979 3144 3193 3212 3276 3518 3569 Size: 201 156 165 49 19 64 242 51 Cuts at: 3569 3647 4451 5048 5835 6103 1239 Size: 78 804 597 787 268 1524 Fragments arranged by size: 1524 804 787 597 501 393 268 242 201 165 161 158 156 78 64 64 56 51 49 43 19 7 EcoNI CCTnn'n_nnAGG Cuts at: 3181 3181 Size: 6388 EcoO109I rG'GnC_Cy Cuts at: 3065 3333 3354 3544 3571 3065 Size: 268 21 190 27 5882 Fragments arranged by size: 5882 268 190 27 21 EcoRI G'AATT_C Cuts at: 3697 4075 3697 Size: 378 6010 FspI TGC'GCA Cuts at: 4894 4894 Size: 6388 HaeII r_GCGC'y Cuts at: 1678 3272 5535 1678 Size: 1594 2263 2531 Fragments arranged by size: 2531 2263 1594 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2015 2047 3575 3607 5029 Size: 32 135 32 443 32 1528 32 1422 Cuts at: 5029 5061 5103 5135 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1528 1422 443 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3732 3978 669 Size: 988 281 1794 246 3079 Fragments arranged by size: 3079 1794 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6388 HpaI GTT'AAC Cuts at: 3978 3978 Size: 6388 KpnI G_GTAC'C Cuts at: 3713 3713 Size: 6388 MfeI C'AATT_G Cuts at: 3987 3987 Size: 6388 MluI A'CGCG_T Cuts at: 2056 6264 2056 Size: 4208 2180 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2383 2697 5502 5686 1305 Size: 1078 314 2805 184 2007 Fragments arranged by size: 2805 2007 1078 314 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2163 10 Size: 54 1425 343 331 4235 Fragments arranged by size: 4235 1425 343 331 54 MslI CAynn'nnrTG Cuts at: 518 1166 2312 3101 3486 4346 4705 4864 518 Size: 648 1146 789 385 860 359 159 2042 Fragments arranged by size: 2042 1146 860 789 648 385 359 159 MspA1I CmG'CkG Cuts at: 1606 2072 2089 3640 3804 4481 5629 5874 1606 Size: 466 17 1551 164 677 1148 245 2120 Fragments arranged by size: 2120 1551 1148 677 466 245 164 17 NcoI C'CATG_G Cuts at: 513 1065 2004 2194 2745 3426 513 Size: 552 939 190 551 681 3475 Fragments arranged by size: 3475 939 681 552 551 190 NdeI CA'TA_TG Cuts at: 387 387 Size: 6388 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6388 NotI GC'GGCC_GC Cuts at: 3835 3835 Size: 6388 NspI r_CATG'y Cuts at: 1132 1229 3746 5291 1132 Size: 97 2517 1545 2229 Fragments arranged by size: 2517 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2870 3124 1348 Size: 1522 254 4612 Fragments arranged by size: 4612 1522 254 PciI A'CATG_T Cuts at: 5287 5287 Size: 6388 PflMI CCAn_nnn'nTGG Cuts at: 1108 2890 3272 1108 Size: 1782 382 4224 Fragments arranged by size: 4224 1782 382 PmeI GTTT'AAAC Cuts at: 3692 3692 Size: 6388 PmlI CAC'GTG Cuts at: 3601 3601 Size: 6388 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4457 4489 5970 6002 4457 Size: 32 1481 32 4843 Fragments arranged by size: 4843 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3065 3544 3065 Size: 479 5909 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6388 PsiI TTA'TAA Cuts at: 3958 3958 Size: 6388 PspXI vC'TCGA_Gb Cuts at: 2414 2414 Size: 6388 PstI C_TGCA'G Cuts at: 830 2859 3283 3304 3740 830 Size: 2029 424 21 436 3478 Fragments arranged by size: 3478 2029 436 424 21 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6388 PvuII CAG'CTG Cuts at: 3640 3640 Size: 6388 SacI G_AGCT'C Cuts at: 2415 2415 Size: 6388 SacII CC_GC'GG Cuts at: 2090 2090 Size: 6388 SalI G'TCGA_C Cuts at: 1936 3730 1936 Size: 1794 4594 SbfI CC_TGCA'GG Cuts at: 3740 3740 Size: 6388 ScaI AGT'ACT Cuts at: 4636 6348 4636 Size: 1712 4676 SfcI C'TryA_G Cuts at: 826 1045 2652 2855 3279 3300 3736 4871 5552 Size: 219 1607 203 424 21 436 1135 681 Cuts at: 5552 5743 826 Size: 191 1471 Fragments arranged by size: 1607 1471 1135 681 436 424 219 203 191 21 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6388 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6388 SmaI CCC'GGG Cuts at: 1872 3715 1872 Size: 1843 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2414 2532 3254 3443 4492 5393 Size: 197 926 471 118 722 189 1049 901 Cuts at: 5393 5655 820 Size: 262 1553 Fragments arranged by size: 1553 1049 926 901 722 471 262 197 189 118 SnaBI TAC'GTA Cuts at: 493 4179 493 Size: 3686 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6388 SphI G_CATG'C Cuts at: 1132 3746 1132 Size: 2614 3774 SspI AAT'ATT Cuts at: 5 52 3473 4312 5 Size: 47 3421 839 2081 Fragments arranged by size: 3421 2081 839 47 StuI AGG'CCT Cuts at: 1517 2617 1517 Size: 1100 5288 StyI C'CwwG_G Cuts at: 513 1065 2004 2194 2745 3329 3426 3827 513 Size: 552 939 190 551 584 97 401 3074 Fragments arranged by size: 3074 939 584 552 551 401 190 97 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2651 2741 4415 4432 4585 4770 5215 Size: 1255 1071 90 1674 17 153 185 445 Cuts at: 5215 325 Size: 1498 Fragments arranged by size: 1674 1498 1255 1071 445 185 153 90 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4634 6346 96 Size: 274 80 33 51 1443 2657 1712 138 Fragments arranged by size: 2657 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2143 2175 6348 6380 2143 Size: 32 4173 32 2151 Fragments arranged by size: 4173 2151 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3587 1316 Size: 2271 4117 XbaI T'CTAG_A Cuts at: 3724 3724 Size: 6388 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2242 3424 2242 Size: 1182 5206 XhoI C'TCGA_G Cuts at: 1943 2414 1943 Size: 471 5917 XmaI C'CCGG_G Cuts at: 1870 3713 1870 Size: 1843 4545 XmnI GAAnn'nnTTC Cuts at: 4517 4517 Size: 6388 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4196 276 Size: 53 83 186 936 2662 2468 Fragments arranged by size: 2662 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspOMI PsrI RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI