(Circular) (Six-base) MAPSORT of: pFN21AE6746.seq Check: 5903 from: 1 to: 8269 pFN21AE6746 8269 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3256 5297 3256 Size: 2041 6228 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6079 278 Size: 53 83 186 936 4543 2468 Fragments arranged by size: 4543 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3164 5612 1937 Size: 1227 2448 4594 Fragments arranged by size: 4594 2448 1227 Acc65I G'GTAC_C Cuts at: 5590 5590 Size: 8269 AclI AA'CG_TT Cuts at: 1563 4732 6396 6769 1563 Size: 3169 1664 373 3063 Fragments arranged by size: 3169 3063 1664 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3372 3738 4331 6332 7716 7984 1632 Size: 1740 366 593 2001 1384 268 1917 Fragments arranged by size: 2001 1917 1740 1384 593 366 268 AflII C'TTAA_G Cuts at: 820 1017 2036 820 Size: 197 1019 7053 Fragments arranged by size: 7053 1019 197 AflIII A'CryG_T Cuts at: 1203 2684 7168 8145 1203 Size: 1481 4484 977 1327 Fragments arranged by size: 4484 1481 1327 977 AgeI A'CCGG_T Cuts at: 7100 7100 Size: 8269 AhdI GACnn_n'nnGTC Cuts at: 4977 6998 4977 Size: 2021 6248 AleI CACnn'nnGTG Cuts at: 1166 2366 4363 4506 1166 Size: 1200 1997 143 4929 Fragments arranged by size: 4929 1997 1200 143 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4381 5471 5496 1184 Size: 3197 1090 25 3957 Fragments arranged by size: 3957 3197 1090 25 AlwNI CAG_nnn'CTG Cuts at: 2956 5323 6012 7584 2956 Size: 2367 689 1572 3641 Fragments arranged by size: 3641 2367 1572 689 ApaI G_GGCC'C Cuts at: 2733 3010 2733 Size: 277 7992 ApaLI G'TGCA_C Cuts at: 6326 7482 6326 Size: 1156 7113 ApoI r'AATT_y Cuts at: 1493 2204 2328 2896 3707 4012 4258 5078 5578 Size: 711 124 568 811 305 246 820 500 Cuts at: 5578 5804 5956 8065 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 820 811 711 568 500 305 246 226 152 124 AseI AT'TA_AT Cuts at: 160 1032 4143 6823 160 Size: 872 3111 2680 1606 Fragments arranged by size: 3111 2680 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8269 AvaI C'yCGr_G Cuts at: 1870 1943 2130 5337 5594 1870 Size: 73 187 3207 257 4545 Fragments arranged by size: 4545 3207 257 187 73 BamHI G'GATC_C Cuts at: 2064 5599 5966 2064 Size: 3535 367 4367 Fragments arranged by size: 4367 3535 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2161 2368 4469 5590 7045 Size: 325 217 627 374 207 2101 1121 1455 Cuts at: 7045 618 Size: 1842 Fragments arranged by size: 2101 1842 1455 1121 627 374 325 217 207 BanII G_rGCy'C Cuts at: 1391 2733 2999 3010 3976 5207 5588 1391 Size: 1342 266 11 966 1231 381 4072 Fragments arranged by size: 4072 1342 1231 966 381 266 11 BbeI G_GCGC'C Cuts at: 4473 4473 Size: 8269 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3378 3619 5429 928 Size: 971 1479 241 1810 3768 Fragments arranged by size: 3768 1810 1479 971 241 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 6460 6494 1903 Size: 34 50 34 4439 34 3678 Fragments arranged by size: 4439 3678 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3077 6161 7371 3077 Size: 3084 1210 3975 Fragments arranged by size: 3975 3084 1210 BclI T'GATC_A Cuts at: 1548 2376 4290 5403 1548 Size: 828 1914 1113 4414 Fragments arranged by size: 4414 1914 1113 828 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4223 6880 136 Size: 107 122 71 631 605 2551 2657 1525 Fragments arranged by size: 2657 2551 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2808 8264 2808 Size: 5456 2813 BlpI GC'TnA_GC Cuts at: 5686 5686 Size: 8269 Bme1580I G_kGCm'C Cuts at: 1790 2733 3010 6330 7486 1790 Size: 943 277 3320 1156 2573 Fragments arranged by size: 3320 2573 1156 943 277 BmgBI CAC'GTC Cuts at: 1318 2139 4282 1318 Size: 821 2143 5305 Fragments arranged by size: 5305 2143 821 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3196 6000 6958 Size: 416 53 83 399 24 1779 2804 958 Cuts at: 6958 442 Size: 1753 Fragments arranged by size: 2804 1779 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8269 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2177 4633 4973 5154 5327 6929 1239 Size: 938 2456 340 181 173 1602 2579 Fragments arranged by size: 2579 2456 1602 938 340 181 173 Bpu10I CC'TnA_GC Cuts at: 4826 5201 4826 Size: 375 7894 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2900 3153 3170 5475 6394 7259 7557 2900 Size: 253 17 2305 919 865 298 3612 Fragments arranged by size: 3612 2305 919 865 298 253 17 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2142 3030 5054 6932 882 Size: 617 643 888 2024 1878 2219 Fragments arranged by size: 2219 2024 1878 888 643 617 BsaAI yAC'GTr Cuts at: 493 1204 2687 4504 6060 493 Size: 711 1483 1817 1556 2702 Fragments arranged by size: 2702 1817 1556 1483 711 BsaBI GATnn'nnATC Cuts at: 3474 4168 8263 3474 Size: 694 4095 3480 Fragments arranged by size: 4095 3480 694 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4470 5426 5466 6076 Size: 53 83 186 936 2937 956 40 610 Cuts at: 6076 6458 7093 275 Size: 382 635 1451 Fragments arranged by size: 2937 1451 956 936 635 610 382 186 83 53 40 BsaWI w'CCGG_w Cuts at: 4335 6702 7100 7374 7521 7932 4335 Size: 2367 398 274 147 411 4672 Fragments arranged by size: 4672 2367 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2514 2544 4307 4337 1178 Size: 30 165 30 1111 30 1763 30 5110 Fragments arranged by size: 5110 1763 1111 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3402 3938 4354 4386 5324 1186 Size: 2216 536 416 32 938 4131 Fragments arranged by size: 4131 2216 938 536 416 32 BseYI C'CCAG_C Cuts at: 3616 4264 4836 5413 7472 7968 3616 Size: 648 572 577 2059 496 3917 Fragments arranged by size: 3917 2059 648 577 572 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2969 3352 4228 5336 1163 Size: 573 1233 383 876 1108 4096 Fragments arranged by size: 4096 1233 1108 876 573 383 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5398 5719 6480 7508 1540 Size: 275 186 3397 321 761 1028 2301 Fragments arranged by size: 3397 2301 1028 761 321 275 186 BsiHKAI G_wGCw'C Cuts at: 2999 3976 5207 6330 6415 7486 2999 Size: 977 1231 1123 85 1071 3782 Fragments arranged by size: 3782 1231 1123 1071 977 85 BsmI GAATG_Cn' Cuts at: 3492 5780 5873 3492 Size: 2288 93 5888 Fragments arranged by size: 5888 2288 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2627 4458 2627 Size: 1831 6438 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2733 2999 3010 3976 5207 5588 6330 Size: 399 943 266 11 966 1231 381 742 Cuts at: 6330 6415 7486 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1231 1071 966 943 742 399 381 266 85 11 BspEI T'CCGG_A Cuts at: 4335 7932 4335 Size: 3597 4672 BspHI T'CATG_A Cuts at: 3315 6158 7888 3315 Size: 2843 1730 3696 Fragments arranged by size: 3696 2843 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3256 5297 5624 844 Size: 2412 2041 327 3489 Fragments arranged by size: 3489 2412 2041 327 BsrBI CCG'CTC Cuts at: 1394 1823 2070 6156 1394 Size: 429 247 4086 3507 Fragments arranged by size: 4086 3507 429 247 BsrDI GCAATG_nn' Cuts at: 65 1248 2310 2604 3336 5163 5300 5377 6764 Size: 1183 1062 294 732 1827 137 77 1387 Cuts at: 6764 6938 65 Size: 174 1396 Fragments arranged by size: 1827 1396 1387 1183 1062 732 294 174 137 77 BsrFI r'CCGG_y Cuts at: 1700 1810 6913 7100 1700 Size: 110 5103 187 2869 Fragments arranged by size: 5103 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4749 96 Size: 4653 3616 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8269 BssSI C'ACGA_G Cuts at: 4363 6329 7341 4363 Size: 1966 1012 5291 Fragments arranged by size: 5291 1966 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2444 794 Size: 1650 6619 BstBI TT'CG_AA Cuts at: 3745 4329 7073 3745 Size: 584 2744 4941 Fragments arranged by size: 4941 2744 584 BstEII G'GTnAC_C Cuts at: 1184 4627 1184 Size: 3443 4826 BstYI r'GATC_y Cuts at: 1972 2064 2808 2952 5599 5966 6351 6368 7820 Size: 92 744 144 2647 367 385 17 1452 Cuts at: 7820 7906 7918 8264 1972 Size: 86 12 346 1977 Fragments arranged by size: 2647 1977 1452 744 385 367 346 144 92 86 17 12 BstZ17I GTA'TAC Cuts at: 3165 3165 Size: 8269 Bsu36I CC'TnA_GG Cuts at: 2592 2592 Size: 8269 BtgI C'CryG_G Cuts at: 513 1065 2051 2076 3228 3864 513 Size: 552 986 25 1152 636 4918 Fragments arranged by size: 4918 1152 986 636 552 25 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3680 4060 4549 487 Size: 683 56 2454 380 489 4207 Fragments arranged by size: 4207 2454 683 489 380 56 BtsI GCAGTG_nn' Cuts at: 2523 2667 4240 4894 5785 6579 6599 2523 Size: 144 1573 654 891 794 20 4193 Fragments arranged by size: 4193 1573 891 794 654 144 20 ClaI AT'CG_AT Cuts at: 1554 4296 1554 Size: 2742 5527 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2125 2160 529 Size: 35 1561 35 6638 Fragments arranged by size: 6638 1561 35 35 DraI TTT'AAA Cuts at: 5573 5573 Size: 8269 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7276 809 Size: 507 5960 1802 Fragments arranged by size: 5960 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2229 3231 3516 Size: 54 1425 50 275 18 399 1002 285 Cuts at: 3516 4300 5716 6605 8234 8 Size: 784 1416 889 1629 43 Fragments arranged by size: 1629 1425 1416 1002 889 784 399 285 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5716 1537 Size: 275 3904 4090 Fragments arranged by size: 4090 3904 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2278 2312 3249 3580 3981 4934 6199 1353 Size: 925 34 937 331 401 953 1265 3423 Fragments arranged by size: 3423 1265 953 937 925 401 331 34 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6856 7230 7376 387 Size: 1064 138 5267 374 146 1280 Fragments arranged by size: 5267 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2997 3974 5205 2997 Size: 977 1231 6061 Fragments arranged by size: 6061 1231 977 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2177 3372 3738 4331 4633 4973 5154 Size: 393 545 1195 366 593 302 340 181 Cuts at: 5154 5327 6332 6929 7716 7984 1239 Size: 173 1005 597 787 268 1524 Fragments arranged by size: 1524 1195 1005 787 597 593 545 393 366 340 302 268 181 173 EcoNI CCTnn'n_nnAGG Cuts at: 4196 5459 4196 Size: 1263 7006 EcoO109I rG'GnC_Cy Cuts at: 2081 2729 3006 3007 2081 Size: 648 277 1 7343 Fragments arranged by size: 7343 648 277 1 EcoRI G'AATT_C Cuts at: 2896 5578 5956 2896 Size: 2682 378 5209 Fragments arranged by size: 5209 2682 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3637 4067 4657 3637 Size: 430 590 7249 Fragments arranged by size: 7249 590 430 FspI TGC'GCA Cuts at: 6775 6775 Size: 8269 HaeII r_GCGC'y Cuts at: 1678 2912 4473 7416 1678 Size: 1234 1561 2943 2531 Fragments arranged by size: 2943 2531 1561 1234 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2111 2143 3053 3085 3397 Size: 32 135 32 539 32 910 32 312 Cuts at: 3397 3429 3543 3575 4963 4995 6910 6942 6984 Size: 32 114 32 1388 32 1915 32 42 Cuts at: 6984 7016 1373 Size: 32 2626 Fragments arranged by size: 2626 1915 1388 910 539 312 135 114 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2247 2452 4116 4971 5613 5859 Size: 988 281 309 205 1664 855 642 246 Cuts at: 5859 669 Size: 3079 Fragments arranged by size: 3079 1664 988 855 642 309 281 246 205 HindIII A'AGCT_T Cuts at: 748 4589 748 Size: 3841 4428 HpaI GTT'AAC Cuts at: 2452 5859 2452 Size: 3407 4862 KasI G'GCGC_C Cuts at: 4469 4469 Size: 8269 KpnI G_GTAC'C Cuts at: 5594 5594 Size: 8269 MfeI C'AATT_G Cuts at: 2391 5868 2391 Size: 3477 4792 MluI A'CGCG_T Cuts at: 8145 8145 Size: 8269 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2125 2369 3383 3963 4984 7383 7567 1305 Size: 820 244 1014 580 1021 2399 184 2007 Fragments arranged by size: 2399 2007 1021 1014 820 580 244 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3233 4302 10 Size: 54 1425 343 1401 1069 3977 Fragments arranged by size: 3977 1425 1401 1069 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2366 2745 3197 4363 4506 5478 6227 Size: 648 1200 379 452 1166 143 972 749 Cuts at: 6227 6586 6745 518 Size: 359 159 2042 Fragments arranged by size: 2042 1200 1166 972 749 648 452 379 359 159 143 MspA1I CmG'CkG Cuts at: 1606 2053 2155 5685 6362 7510 7755 1606 Size: 447 102 3530 677 1148 245 2120 Fragments arranged by size: 3530 2120 1148 677 447 245 102 NarI GG'CG_CC Cuts at: 4470 4470 Size: 8269 NcoI C'CATG_G Cuts at: 513 1065 3228 513 Size: 552 2163 5554 Fragments arranged by size: 5554 2163 552 NdeI CA'TA_TG Cuts at: 387 4379 5023 387 Size: 3992 644 3633 Fragments arranged by size: 3992 3633 644 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8269 NotI GC'GGCC_GC Cuts at: 5716 5716 Size: 8269 NspI r_CATG'y Cuts at: 1132 1229 2680 3309 4537 4825 5110 5627 7172 Size: 97 1451 629 1228 288 285 517 1545 Cuts at: 7172 1132 Size: 2229 Fragments arranged by size: 2229 1545 1451 1228 629 517 288 285 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8269 PciI A'CATG_T Cuts at: 7168 7168 Size: 8269 PflMI CCAn_nnn'nTGG Cuts at: 1108 2502 5070 1108 Size: 1394 2568 4307 Fragments arranged by size: 4307 2568 1394 PfoI T'CCnGG_A Cuts at: 3702 4347 5558 3702 Size: 645 1211 6413 Fragments arranged by size: 6413 1211 645 PmeI GTTT'AAAC Cuts at: 5573 5573 Size: 8269 PmlI CAC'GTG Cuts at: 2687 4504 2687 Size: 1817 6452 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4673 4705 6338 6370 7851 7883 4673 Size: 32 1633 32 1481 32 5059 Fragments arranged by size: 5059 1633 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2081 2081 Size: 8269 PshAI GACnn'nnGTC Cuts at: 1613 3399 1613 Size: 1786 6483 PsiI TTA'TAA Cuts at: 5839 5839 Size: 8269 PspOMI G'GGCC_C Cuts at: 2729 3006 2729 Size: 277 7992 PstI C_TGCA'G Cuts at: 830 4388 4404 5460 5621 830 Size: 3558 16 1056 161 3478 Fragments arranged by size: 3558 3478 1056 161 16 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8269 PvuII CAG'CTG Cuts at: 2155 2155 Size: 8269 SacI G_AGCT'C Cuts at: 2999 3976 5207 2999 Size: 977 1231 6061 Fragments arranged by size: 6061 1231 977 SacII CC_GC'GG Cuts at: 2054 2054 Size: 8269 SalI G'TCGA_C Cuts at: 1936 5611 1936 Size: 3675 4594 SapI GCTCTTCn'nnn_ Cuts at: 2278 3249 2278 Size: 971 7298 SbfI CC_TGCA'GG Cuts at: 5621 5621 Size: 8269 ScaI AGT'ACT Cuts at: 6517 8229 6517 Size: 1712 6557 SfcI C'TryA_G Cuts at: 826 1045 2715 3418 4384 4400 5456 5617 6752 Size: 219 1670 703 966 16 1056 161 1135 Cuts at: 6752 7433 7624 826 Size: 681 191 1471 Fragments arranged by size: 1670 1471 1135 1056 966 703 681 219 191 161 16 SfoI GGC'GCC Cuts at: 4471 4471 Size: 8269 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8269 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8269 SmaI CCC'GGG Cuts at: 1872 2132 5596 1872 Size: 260 3464 4545 Fragments arranged by size: 4545 3464 260 SmlI C'TyrA_G Cuts at: 820 1017 1943 2036 2915 3149 3168 5337 5490 Size: 197 926 93 879 234 19 2169 153 Cuts at: 5490 6373 7274 7536 820 Size: 883 901 262 1553 Fragments arranged by size: 2169 1553 926 901 883 879 262 234 197 153 93 19 SnaBI TAC'GTA Cuts at: 493 6060 493 Size: 5567 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8269 SphI G_CATG'C Cuts at: 1132 4537 4825 5110 5627 1132 Size: 3405 288 285 517 3774 Fragments arranged by size: 3774 3405 517 288 285 SspI AAT'ATT Cuts at: 5 52 5011 6193 5 Size: 47 4959 1182 2081 Fragments arranged by size: 4959 2081 1182 47 StuI AGG'CCT Cuts at: 1517 2022 3572 1517 Size: 505 1550 6214 Fragments arranged by size: 6214 1550 505 StyI C'CwwG_G Cuts at: 513 1065 2794 2988 3228 4252 4908 5708 513 Size: 552 1729 194 240 1024 656 800 3074 Fragments arranged by size: 3074 1729 1024 800 656 552 240 194 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4099 4496 4868 6296 6313 6466 6651 Size: 1255 2519 397 372 1428 17 153 185 Cuts at: 6651 7096 325 Size: 445 1498 Fragments arranged by size: 2519 1498 1428 1255 445 397 372 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2235 3012 4644 Size: 274 80 33 51 1443 258 777 1632 Cuts at: 4644 4749 6515 8227 96 Size: 105 1766 1712 138 Fragments arranged by size: 1766 1712 1632 1443 777 274 258 138 105 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2514 2546 3520 3552 3871 3903 5340 5372 8229 Size: 32 974 32 319 32 1437 32 2857 Cuts at: 8229 8261 2514 Size: 32 2522 Fragments arranged by size: 2857 2522 1437 974 319 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8269 XbaI T'CTAG_A Cuts at: 5605 5605 Size: 8269 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3136 4948 3136 Size: 1812 6457 XhoI C'TCGA_G Cuts at: 1943 5337 1943 Size: 3394 4875 XmaI C'CCGG_G Cuts at: 1870 2130 5594 1870 Size: 260 3464 4545 Fragments arranged by size: 4545 3464 260 XmnI GAAnn'nnTTC Cuts at: 3751 6398 3751 Size: 2647 5622 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6077 276 Size: 53 83 186 936 4543 2468 Fragments arranged by size: 4543 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstXI DraIII EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SanDI SexAI SfiI SrfI SwaI