Restriction map for
pFN21AE9633
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# NO CUT ENZYMES ( 67 enzymes) #
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AclI AgeI AscI AseI AsnI
AvrII BbrPI BglII BlnI BsaBI
BsiEI BsiWI BsmI BspDI BspHI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I ClaI DraI DraIII
DrdI EagI EclXI Eco52I EcoO65I
EcoRI EcoRV EcoT22I FseI HpaI
MamI MfeI MluI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
SalI SapI SbfI SfiI SfuI
SgrAI SpeI Sse8387I SspI SwaI
XbaI XhoI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AatII 379 GACGTC GACGT^C
Acc65I 752 GGTACC G^GTACC
AflII 2098 CTTAAG C^TTAAG
AhdI 3371 GACAGCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1127 AGCGCT AGC^GCT
Asp700I 1899 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 752 GGTACC G^GTACC
AspEI 3371 GACAGCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1413 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 935 GGATCC G^GATCC
BcgI 2345 CGAAGAAGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3104 GTATCC GTATCC(6/5)
BfrI 2098 CTTAAG C^TTAAG
Bsp1407I 509 TGTACA T^GTACA
BspLU11I 602 ACATGT A^CATGT
BsrBI 2730 CCGCTC CCGCTC(-3/-3)
BsrGI 509 TGTACA T^GTACA
BssSI 2866 CACGAG CACGAG(-5/-1)
CpoI 1861 CGGACCG CG^G[AT]CCG
Eam1105I 3371 GACAGCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1127 AGCGCT AGC^GCT
HincII 2125 GTCAAC GT[CT]^[GA]AC
HindII 2125 GTCAAC GT[CT]^[GA]AC
KpnI 752 GGTACC GGTAC^C
NcoI 257 CCATGG C^CATGG
PflFI 1413 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3540 CCAGTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1675 GACCCTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 1861 CGGACCG CG^G[AT]CCG
ScaI 683 AGTACT AGT^ACT
SexAI 581 ACCTGGT A^CC[AT]GGT
SnaBI 286 TACGTA TAC^GTA
SphI 3254 GCATGC GCATG^C
SspBI 509 TGTACA T^GTACA
Tth111I 1413 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3540 CCAGTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1899 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AccIII 3524 TCCGGA T^CCGGA
3635 TCCGGA
AcsI 106 GAATTT [GA]^AATT[CT]
1057 AAATTC
AflIII 602 ACATGT A^C[GA][CT]GT
1109 ACGTGT
Alw44I 250 GTGCAC G^TGCAC
2392 GTGCAC
ApaLI 250 GTGCAC G^TGCAC
2392 GTGCAC
ApoI 106 GAATTT [GA]^AATT[CT]
1057 AAATTC
AviII 2372 TGCGCA TGC^GCA
3047 TGCGCA
BclI 1245 TGATCA T^GATCA
3846 TGATCA
BsaAI 286 TACGTA [CT]AC^GT[GA]
1108 TACGTG
BseAI 3524 TCCGGA T^CCGGA
3635 TCCGGA
BspEI 3524 TCCGGA T^CCGGA
3635 TCCGGA
BsrDI 651 CATTGC GCAATG(2/0)
2072 GCAATG
BstXI 1448 CCACCACATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2140 CCAAACTGCTGG
EarI 1206 CTCTTC CTCTTC(1/4)
2023 GAAGAG
EcoNI 1137 CCTCACTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2238 CCTGCTCAAGG
FbaI 1245 TGATCA T^GATCA
3846 TGATCA
FspI 2372 TGCGCA TGC^GCA
3047 TGCGCA
HindIII 217 AAGCTT A^AGCTT
2587 AAGCTT
Ksp632I 1206 CTCTTC CTCTTC(1/4)
2023 GAAGAG
MroI 3524 TCCGGA T^CCGGA
3635 TCCGGA
NaeI 401 GCCGGC GCC^GGC
2173 GCCGGC
NgoMIV 401 GCCGGC G^CCGGC
2173 GCCGGC
PstI 26 CTGCAG CTGCA^G
3264 CTGCAG
SacI 1814 GAGCTC GAGCT^C
2857 GAGCTC
SmlI 2098 CTTAAG C^T[CT][GA]AG
2242 CTCAAG
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# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
AspHI AvaI AvaII BalI BanI
BanII BbeI BbsI BbvCI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
KspI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PvuII RsaI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmaI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII XmaCI XmaI