################################## # NO CUT ENZYMES ( 151 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BanI BanII BbeI BbrPI BcgI BcnI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsoBI Bsp1286I BspDI BspEI BspMI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI FauI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HindIII HpaI HpaII KasI KpnI KspI MamI MluI MroI MspI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AcsI 249 GAATTT [GA]^AATT[CT] ApoI 249 GAATTT [GA]^AATT[CT] Asp700I 705 GAAGGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaII 758 GGACC G^G[AT]CC BalI 371 TGGCCA TGG^CCA BbvCI 691 GCTGAGG CCTCAGC(-5/-2) BbvI 305 GCTGC GCAGC(8/12) BciVI 153 GGATAC GTATCC(6/5) BclI 839 TGATCA T^GATCA BseRI 550 CTCCTC GAGGAG(10/8) BsmFI 757 GGGAC GGGAC(10/14) Bsp1407I 257 TGTACA T^GTACA BspHI 320 TCATGA T^CATGA BspLU11I 653 ACATGT A^CATGT BsrBI 149 GAGCGG CCGCTC(-3/-3) BsrGI 257 TGTACA T^GTACA BstYI 593 GGATCT [GA]^GATC[CT] Cac8I 735 GCTGGC GC[ACGT]^[ACGT]GC DraII 757 GGGACCC [GA]G^G[ACGT]CC[CT] DsaI 350 CCATGG C^C[GA][CT]GG EaeI 371 TGGCCA [CT]^GGCC[GA] EcoO109I 757 GGGACCC [GA]G^G[ACGT]CC[CT] EcoRV 324 GATATC GAT^ATC EcoT14I 350 CCATGG C^C[AT][AT]GG FbaI 839 TGATCA T^GATCA Fnu4HI 305 GCTGC GC^[ACGT]GC HincII 520 GTCAAC GT[CT]^[GA]AC HindII 520 GTCAAC GT[CT]^[GA]AC ItaI 305 GCTGC GC^[ACGT]GC MaeII 718 ACGT A^CGT MfeI 12 CAATTG C^AATTG MflI 593 GGATCT [GA]^GATC[CT] MluNI 371 TGGCCA TGG^CCA MscI 371 TGGCCA TGG^CCA MseI 862 TTAA T^TAA MspA1I 689 CAGCTG C[AC]G^C[GT]G MunI 12 CAATTG C^AATTG MwoI 346 GCTTCCATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NcoI 350 CCATGG C^CATGG PflMI 795 CCATGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 408 GACTC GAGTC(4/5) PpuMI 757 GGGACCC [GA]G^G[AT]CC[CT] PvuII 689 CAGCTG CAG^CTG RcaI 320 TCATGA T^CATGA SspBI 257 TGTACA T^GTACA StyI 350 CCATGG C^C[AT][AT]GG TaqI 715 TCGA T^CGA Tru9I 862 TTAA T^TAA TseI 305 GCTGC G^C[AT]GC Tsp45I 742 GTCAC ^GT[GC]AC TthHB8I 715 TCGA T^CGA Van91I 795 CCATGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 593 GGATCT [GA]^GATC[CT] XmnI 705 GAAGGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AciI 492 CCGC CCGC(-3/-1) 151 GCGG AflIII 653 ACATGT A^C[GA][CT]GT 718 ACGTGT AlwI 135 GATCC GGATC(4/5) 593 GGATC AocI 552 CCTCAGG CC^T[ACGT]AGG 675 CCTGAGG BbsI 698 GAAGAC GAAGAC(2/6) 826 GTCTTC BpmI 410 CTCCAG CTGGAG(16/14) 874 CTCCAG BpuAI 698 GAAGAC GAAGAC(2/6) 826 GTCTTC BsmAI 161 GTCTC GTCTC(1/5) 646 GTCTC BsmI 422 GCATTC GAATGC(1/-1) 684 GCATTC BsrI 300 ACTGG ACTGG(1/-1) 342 ACTGG Bsu36I 552 CCTCAGG CC^T[ACGT]AGG 675 CCTGAGG Cfr13I 45 GGGCC G^G[ACGT]CC 758 GGACC EarI 106 CTCTTC CTCTTC(1/4) 219 CTCTTC Eco81I 552 CCTCAGG CC^T[ACGT]AGG 675 CCTGAGG EcoT22I 284 ATGCAT ATGCA^T 682 ATGCAT Ksp632I 106 CTCTTC CTCTTC(1/4) 219 CTCTTC NlaIV 757 GGGACC GG[ACGT]^[ACGT]CC 758 GGACCC NsiI 284 ATGCAT ATGCA^T 682 ATGCAT Sau96I 45 GGGCC G^G[ACGT]CC 758 GGACC SfcI 450 CTATAG C^T[GA][CT]AG 867 CTATAG Tsp509I 13 AATT ^AATT 250 AATT TspRI 113 CAGTG CA[GC]TG[ACGT][ACGT]^ 836 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 31 enzymes) # ######################################### AfaI AluI BfaI BsaJI BsiYI BslI BssKI BstF5I BstNI DdeI DpnII EcoRII FokI HaeIII HinfI HphI MaeI MaeIII MboI MboII MnlI MslI MvaI NdeII NlaIII NspI RsaI Sau3AI ScrFI SfaNI TfiI