(Linear) (Six-base) MAPSORT of: KIEE5393.seq Check: 5582 from: 1 to: 927 KIEE5393.orf With 182 enzymes: SgfI * January 29, 2014 11:58 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 922 927 Size: 922 5 AflIII A'CryG_T Cuts at: 0 653 718 927 Size: 653 65 209 Fragments arranged by size: 653 209 65 ApoI r'AATT_y Cuts at: 0 249 927 Size: 249 678 BbsI GAAGACnn'nnnn_ Cuts at: 0 705 819 927 Size: 705 114 108 Fragments arranged by size: 705 114 108 BbvCI CC'TCA_GC Cuts at: 0 692 927 Size: 692 235 BciVI GTATCCnnnnn_n' Cuts at: 0 147 927 Size: 147 780 BclI T'GATC_A Cuts at: 0 839 927 Size: 839 88 BfrBI ATG'CAT Cuts at: 0 286 684 927 Size: 286 398 243 Fragments arranged by size: 398 286 243 BmgBI CAC'GTC Cuts at: 0 719 927 Size: 719 208 BmrI ACTGGGnnnn_n' Cuts at: 0 310 927 Size: 310 617 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 395 859 927 Size: 395 464 68 Fragments arranged by size: 464 395 68 Bpu10I CC'TnA_GC Cuts at: 0 692 927 Size: 692 235 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 541 927 Size: 541 386 BsmI GAATG_Cn' Cuts at: 0 422 684 927 Size: 422 262 243 Fragments arranged by size: 422 262 243 BspHI T'CATG_A Cuts at: 0 320 927 Size: 320 607 BsrBI CCG'CTC Cuts at: 0 151 927 Size: 151 776 BsrGI T'GTAC_A Cuts at: 0 257 927 Size: 257 670 BstYI r'GATC_y Cuts at: 0 593 927 Size: 593 334 Bsu36I CC'TnA_GG Cuts at: 0 553 676 927 Size: 553 123 251 Fragments arranged by size: 553 251 123 BtgI C'CryG_G Cuts at: 0 350 927 Size: 350 577 EaeI y'GGCC_r Cuts at: 0 371 927 Size: 371 556 EarI CTCTTCn'nnn_ Cuts at: 0 112 225 927 Size: 112 113 702 Fragments arranged by size: 702 113 112 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 481 927 Size: 481 446 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 395 859 922 927 Size: 395 464 63 5 Fragments arranged by size: 464 395 63 5 EcoO109I rG'GnC_Cy Cuts at: 0 758 927 Size: 758 169 EcoRV GAT'ATC Cuts at: 0 326 927 Size: 326 601 HincII GTy'rAC Cuts at: 0 522 927 Size: 522 405 MfeI C'AATT_G Cuts at: 0 12 927 Size: 12 915 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 536 927 Size: 536 391 MscI TGG'CCA Cuts at: 0 373 927 Size: 373 554 MslI CAynn'nnrTG Cuts at: 0 172 319 769 847 927 Size: 172 147 450 78 80 Fragments arranged by size: 450 172 147 80 78 MspA1I CmG'CkG Cuts at: 0 691 927 Size: 691 236 NcoI C'CATG_G Cuts at: 0 350 927 Size: 350 577 NsiI A_TGCA'T Cuts at: 0 288 686 927 Size: 288 398 241 Fragments arranged by size: 398 288 241 NspI r_CATG'y Cuts at: 0 290 546 657 735 927 Size: 290 256 111 78 192 Fragments arranged by size: 290 256 192 111 78 PciI A'CATG_T Cuts at: 0 653 927 Size: 653 274 PflMI CCAn_nnn'nTGG Cuts at: 0 801 927 Size: 801 126 PfoI T'CCnGG_A Cuts at: 0 411 927 Size: 411 516 PpuMI rG'GwC_Cy Cuts at: 0 758 927 Size: 758 169 PvuII CAG'CTG Cuts at: 0 691 927 Size: 691 236 SanDI GG'GwC_CC Cuts at: 0 758 927 Size: 758 169 SfcI C'TryA_G Cuts at: 0 450 867 927 Size: 450 417 60 Fragments arranged by size: 450 417 60 StyI C'CwwG_G Cuts at: 0 350 927 Size: 350 577 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 34 249 377 927 Size: 34 215 128 550 Fragments arranged by size: 550 215 128 34 TatI w'GTAC_w Cuts at: 0 176 257 773 927 Size: 176 81 516 154 Fragments arranged by size: 516 176 154 81 XmnI GAAnn'nnTTC Cuts at: 0 709 927 Size: 709 218 Enzymes that do cut: AcuI AflIII ApoI BbsI BbvCI BciVI BclI BfrBI BmgBI BmrI BpmI Bpu10I BseRI BsmI BspHI BsrBI BsrGI BstYI Bsu36I BtgI EaeI EarI EciI Eco57MI EcoO109I EcoRV HincII MfeI MmeI MscI MslI MspA1I NcoI NsiI NspI PciI PflMI PfoI PpuMI PvuII SanDI SfcI StyI TaqII TatI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BcgI BglI BglII BlpI Bme1580I BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspMI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoNI EcoRI FalI FseI FspI FspAI HaeII Hin4I HindIII HpaI KasI KpnI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PasI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI Tth111I XbaI XcmI XhoI XmaI ZraI