(Linear) (Six-base) MAPSORT of: KIEE4181.seq Check: 4738 from: 1 to: 1959 KIEE4181.orf With 182 enzymes: SgfI * March 22, 2012 17:32 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 742 1046 1959 Size: 742 304 913 Fragments arranged by size: 913 742 304 AflIII A'CryG_T Cuts at: 0 528 1959 Size: 528 1431 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 754 786 1959 Size: 754 32 1173 Fragments arranged by size: 1173 754 32 AlwNI CAG_nnn'CTG Cuts at: 0 124 127 148 518 1398 1959 Size: 124 3 21 370 880 561 Fragments arranged by size: 880 561 370 124 21 3 ApaI G_GGCC'C Cuts at: 0 81 849 1959 Size: 81 768 1110 Fragments arranged by size: 1110 768 81 ApoI r'AATT_y Cuts at: 0 862 1786 1959 Size: 862 924 173 Fragments arranged by size: 924 862 173 AvaI C'yCGr_G Cuts at: 0 74 80 853 1959 Size: 74 6 773 1106 Fragments arranged by size: 1106 773 74 6 BanII G_rGCy'C Cuts at: 0 31 81 181 849 907 1798 1959 Size: 31 50 100 668 58 891 161 Fragments arranged by size: 891 668 161 100 58 50 31 BbsI GAAGACnn'nnnn_ Cuts at: 0 845 1959 Size: 845 1114 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1599 1633 1959 Size: 1599 34 326 Fragments arranged by size: 1599 326 34 BfrBI ATG'CAT Cuts at: 0 1540 1959 Size: 1540 419 BglII A'GATC_T Cuts at: 0 462 1959 Size: 462 1497 Bme1580I G_kGCm'C Cuts at: 0 81 849 1091 1261 1959 Size: 81 768 242 170 698 Fragments arranged by size: 768 698 242 170 81 BmrI ACTGGGnnnn_n' Cuts at: 0 323 579 1264 1441 1959 Size: 323 256 685 177 518 Fragments arranged by size: 685 518 323 256 177 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 116 1959 Size: 116 1843 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 90 891 1096 1356 1959 Size: 90 801 205 260 603 Fragments arranged by size: 801 603 260 205 90 Bpu10I CC'TnA_GC Cuts at: 0 30 1959 Size: 30 1929 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1765 1959 Size: 1765 194 BsaI GGTCTCn'nnnn_ Cuts at: 0 556 602 1959 Size: 556 46 1357 Fragments arranged by size: 1357 556 46 BsaWI w'CCGG_w Cuts at: 0 1355 1959 Size: 1355 604 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 400 430 754 781 784 811 1213 1243 Size: 400 30 324 27 3 27 402 30 Cuts at: 1243 1867 1897 1959 Size: 624 30 62 Fragments arranged by size: 624 402 400 324 62 30 30 30 27 27 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 264 267 355 382 385 397 400 403 Size: 264 3 88 27 3 12 3 3 Cuts at: 403 412 421 775 802 874 1891 1959 Size: 9 9 354 27 72 1017 68 Fragments arranged by size: 1017 354 264 88 72 68 27 27 12 9 9 3 3 3 3 BseYI C'CCAG_C Cuts at: 0 676 1180 1271 1959 Size: 676 504 91 688 Fragments arranged by size: 688 676 504 91 BsiHKAI G_wGCw'C Cuts at: 0 31 907 1798 1959 Size: 31 876 891 161 Fragments arranged by size: 891 876 161 31 BsmI GAATG_Cn' Cuts at: 0 1749 1959 Size: 1749 210 Bsp1286I G_dGCh'C Cuts at: 0 31 81 181 849 907 1091 1261 1798 Size: 31 50 100 668 58 184 170 537 Cuts at: 1798 1959 Size: 161 Fragments arranged by size: 668 537 184 170 161 100 58 50 31 BspEI T'CCGG_A Cuts at: 0 1355 1959 Size: 1355 604 BspHI T'CATG_A Cuts at: 0 1208 1959 Size: 1208 751 BsrBI CCG'CTC Cuts at: 0 87 1959 Size: 87 1872 BsrDI GCAATG_nn' Cuts at: 0 17 309 654 1296 1634 1652 1959 Size: 17 292 345 642 338 18 307 Fragments arranged by size: 642 345 338 307 292 18 17 BsrFI r'CCGG_y Cuts at: 0 290 1959 Size: 290 1669 BstAPI GCAn_nnn'nTGC Cuts at: 0 124 1027 1959 Size: 124 903 932 Fragments arranged by size: 932 903 124 BstEII G'GTnAC_C Cuts at: 0 1557 1959 Size: 1557 402 BstYI r'GATC_y Cuts at: 0 347 462 1008 1358 1394 1959 Size: 347 115 546 350 36 565 Fragments arranged by size: 565 546 350 347 115 36 Bsu36I CC'TnA_GG Cuts at: 0 352 473 1959 Size: 352 121 1486 Fragments arranged by size: 1486 352 121 BtgI C'CryG_G Cuts at: 0 438 1959 Size: 438 1521 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1925 1959 Size: 1925 34 DraI TTT'AAA Cuts at: 0 867 1959 Size: 867 1092 DraIII CAC_nnn'GTG Cuts at: 0 1027 1959 Size: 1027 932 EaeI y'GGCC_r Cuts at: 0 137 1959 Size: 137 1822 EarI CTCTTCn'nnn_ Cuts at: 0 389 398 718 827 1198 1459 1959 Size: 389 9 320 109 371 261 500 Fragments arranged by size: 500 389 371 320 261 109 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1516 1959 Size: 1516 443 EcoICRI GAG'CTC Cuts at: 0 29 905 1796 1959 Size: 29 876 891 163 Fragments arranged by size: 891 876 163 29 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 90 742 891 1046 1096 1356 1959 Size: 90 652 149 155 50 260 603 Fragments arranged by size: 652 603 260 155 149 90 50 EcoO109I rG'GnC_Cy Cuts at: 0 206 792 845 846 1622 1959 Size: 206 586 53 1 776 337 Fragments arranged by size: 776 586 337 206 53 1 EcoRV GAT'ATC Cuts at: 0 750 1959 Size: 750 1209 FspI TGC'GCA Cuts at: 0 646 1959 Size: 646 1313 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 894 926 1213 1245 1959 Size: 894 32 287 32 714 Fragments arranged by size: 894 714 287 32 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 226 1959 Size: 226 1733 MslI CAynn'nnrTG Cuts at: 0 443 1545 1959 Size: 443 1102 414 Fragments arranged by size: 1102 443 414 MspA1I CmG'CkG Cuts at: 0 115 124 136 145 264 1959 Size: 115 9 12 9 119 1695 Fragments arranged by size: 1695 119 115 12 9 9 NcoI C'CATG_G Cuts at: 0 438 1959 Size: 438 1521 NsiI A_TGCA'T Cuts at: 0 1542 1959 Size: 1542 417 NspI r_CATG'y Cuts at: 0 51 1425 1959 Size: 51 1374 534 Fragments arranged by size: 1374 534 51 PasI CC'CwG_GG Cuts at: 0 1812 1959 Size: 1812 147 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 754 786 1959 Size: 754 32 1173 Fragments arranged by size: 1173 754 32 PpuMI rG'GwC_Cy Cuts at: 0 1622 1959 Size: 1622 337 PshAI GACnn'nnGTC Cuts at: 0 264 1959 Size: 264 1695 PspOMI G'GGCC_C Cuts at: 0 77 845 1959 Size: 77 768 1114 Fragments arranged by size: 1114 768 77 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 150 182 1959 Size: 150 32 1777 Fragments arranged by size: 1777 150 32 PstI C_TGCA'G Cuts at: 0 120 1394 1959 Size: 120 1274 565 Fragments arranged by size: 1274 565 120 PvuII CAG'CTG Cuts at: 0 115 124 145 1959 Size: 115 9 21 1814 Fragments arranged by size: 1814 115 21 9 SacI G_AGCT'C Cuts at: 0 31 907 1798 1959 Size: 31 876 891 161 Fragments arranged by size: 891 876 161 31 SanDI GG'GwC_CC Cuts at: 0 1622 1959 Size: 1622 337 ScaI AGT'ACT Cuts at: 0 631 1959 Size: 631 1328 SfcI C'TryA_G Cuts at: 0 116 423 495 510 1390 1601 1959 Size: 116 307 72 15 880 211 358 Fragments arranged by size: 880 358 307 211 116 72 15 SmaI CCC'GGG Cuts at: 0 76 82 1959 Size: 76 6 1877 Fragments arranged by size: 1877 76 6 SmlI C'TyrA_G Cuts at: 0 1780 1959 Size: 1780 179 SphI G_CATG'C Cuts at: 0 1425 1959 Size: 1425 534 SrfI GCCC'GGGC Cuts at: 0 76 1959 Size: 76 1883 SspI AAT'ATT Cuts at: 0 1097 1959 Size: 1097 862 StuI AGG'CCT Cuts at: 0 551 1388 1959 Size: 551 837 571 Fragments arranged by size: 837 571 551 StyI C'CwwG_G Cuts at: 0 438 1959 Size: 438 1521 TatI w'GTAC_w Cuts at: 0 629 1959 Size: 629 1330 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1849 1867 1881 1899 1959 Size: 1849 18 14 18 60 Fragments arranged by size: 1849 60 18 18 14 Tth111I GACn'n_nGTC Cuts at: 0 1225 1959 Size: 1225 734 XbaI T'CTAG_A Cuts at: 0 1833 1959 Size: 1833 126 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1541 1569 1959 Size: 1541 28 390 Fragments arranged by size: 1541 390 28 XmaI C'CCGG_G Cuts at: 0 74 80 1959 Size: 74 6 1879 Fragments arranged by size: 1879 74 6 Enzymes that do cut: AcuI AflIII AloI AlwNI ApaI ApoI AvaI BanII BbsI BcgI BfrBI BglII Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaWI BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I BspEI BspHI BsrBI BsrDI BsrFI BstAPI BstEII BstYI Bsu36I BtgI CspCI DraI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRV FspI Hin4I MmeI MslI MspA1I NcoI NsiI NspI PasI PpiI PpuMI PshAI PspOMI PsrI PstI PvuII SacI SanDI ScaI SfcI SmaI SmlI SphI SrfI SspI StuI StyI TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BciVI BclI BglI BlpI BmgBI BmtI BsaAI BsaBI BsaHI BsgI BsiEI BsiWI BsmBI BspMI BsrGI BssHII BssSI BstBI BstXI BstZ17I BtgZI BtsI ClaI DrdI EagI EcoNI EcoRI FalI FseI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MscI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PciI PflMI PfoI PmeI PmlI PsiI PspXI PvuI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SwaI TaqII XhoI XmnI ZraI