################################## # NO CUT ENZYMES ( 158 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BanI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BsaAI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmAI BsmBI BsmI BsoBI BspDI BspEI BspHI BspLU11I BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoT22I FbaI FseI FspI HapII HgaI Hin1I HincII HindII HindIII HinfI HpaI HpaII KasI KpnI KspI MfeI MluI MroI MseI MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI TaqI TfiI Tru9I Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AocI 295 CCTAAGG CC^T[ACGT]AGG BamHI 781 GGATCC G^GATCC BanII 229 GAGCCC G[GA]GC[CT]^C BbsI 730 GTCTTC GAAGAC(2/6) BlpI 967 GCTAAGC GC^T[ACGT]AGC BpmI 118 CTGGAG CTGGAG(16/14) Bpu1102I 967 GCTAAGC GC^T[ACGT]AGC BpuAI 730 GTCTTC GAAGAC(2/6) BsaBI 567 GATCAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 553 CTGCAC GTGCAG(16/14) BsmFI 814 GTCCC GGGAC(10/14) BspMI 551 ACCTGC ACCTGC(4/8) BsrBI 756 CCGCTC CCGCTC(-3/-3) BstAPI 860 GCATCCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 295 CCTAAGG CC^T[ACGT]AGG CelII 967 GCTAAGC GC^T[ACGT]AGC DsaI 407 CCATGG C^C[GA][CT]GG Eco81I 295 CCTAAGG CC^T[ACGT]AGG EcoNI 207 CCTCTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 628 GATATC GAT^ATC FauI 755 CCCGC CCCGC(4/6) HaeII 15 GGCGCT [GA]GCGC^[CT] HphI 9 GGTGA GGTGA(8/7) MaeII 899 ACGT A^CGT MamI 567 GATCAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MspA1I 659 CAGCTG C[AC]G^C[GT]G NcoI 407 CCATGG C^CATGG NlaIV 781 GGATCC GG[ACGT]^[ACGT]CC PflMI 308 CCAATTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 659 CAGCTG CAG^CTG SapI 664 GCTCTTC GCTCTTC(1/4) SfcI 840 CTACAG C^T[GA][CT]AG StuI 678 AGGCCT AGG^CCT Van91I 308 CCAATTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AluI 511 AGCT AG^CT 660 AGCT AspHI 547 GAGCAC G[AT]GC[AT]^C 595 GAGCAC BfaI 319 CTAG C^TAG 682 CTAG BsiHKAI 547 GAGCAC G[AT]GC[AT]^C 595 GAGCAC Bsp1407I 161 TGTACA T^GTACA 236 TGTACA BsrGI 161 TGTACA T^GTACA 236 TGTACA BstYI 781 GGATCC [GA]^GATC[CT] 997 GGATCT CfoI 16 GCGC GCG^C 820 GCGC DraII 299 AGGCCCT [GA]G^G[ACGT]CC[CT] 764 AGGCCCT EcoO109I 299 AGGCCCT [GA]G^G[ACGT]CC[CT] 764 AGGCCCT EcoT14I 407 CCATGG C^C[AT][AT]GG 761 CCAAGG HhaI 16 GCGC GCG^C 820 GCGC HinP1I 16 GCGC G^CGC 820 GCGC MaeI 319 CTAG C^TAG 682 CTAG MaeIII 454 GTCAC ^GT[ACGT]AC 490 GTCAC MflI 781 GGATCC [GA]^GATC[CT] 997 GGATCT MwoI 83 GCATAGCAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 860 GCATCCCATGC SmlI 544 CTTGAG C^T[CT][GA]AG 746 CTCAAG SspBI 161 TGTACA T^GTACA 236 TGTACA StyI 407 CCATGG C^C[AT][AT]GG 761 CCAAGG Tsp45I 454 GTCAC ^GT[GC]AC 490 GTCAC Tsp509I 195 AATT ^AATT 310 AATT XhoII 781 GGATCC [GA]^GATC[CT] 997 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 43 enzymes) # ######################################### AciI AfaI AlwI BalI BbvI BmyI BsaJI BsiYI BslI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EaeI EarI EcoRII Fnu4HI FokI HaeIII ItaI Ksp632I MboI MboII MluNI MnlI MscI MslI MvaI NdeII NlaIII RsaI Sau3AI Sau96I ScrFI SfaNI TseI TspRI