(Circular) (Six-base) MAPSORT of: pFN21AE6257.seq Check: 306 from: 1 to: 5446 pFN21AE6257 5446 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3256 278 Size: 53 83 186 936 1720 2468 Fragments arranged by size: 2468 1720 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2789 1937 Size: 852 4594 Acc65I G'GTAC_C Cuts at: 2767 2767 Size: 5446 AclI AA'CG_TT Cuts at: 1563 3573 3946 1563 Size: 2010 373 3063 Fragments arranged by size: 3063 2010 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2466 3509 4893 5161 1632 Size: 834 1043 1384 268 1917 Fragments arranged by size: 1917 1384 1043 834 268 AflII C'TTAA_G Cuts at: 820 1017 2285 820 Size: 197 1268 3981 Fragments arranged by size: 3981 1268 197 AflIII A'CryG_T Cuts at: 1203 4345 5322 1203 Size: 3142 977 1327 Fragments arranged by size: 3142 1327 977 AgeI A'CCGG_T Cuts at: 2187 4277 2187 Size: 2090 3356 AhdI GACnn_n'nnGTC Cuts at: 4175 4175 Size: 5446 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5446 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5446 AlwNI CAG_nnn'CTG Cuts at: 2666 3189 4761 2666 Size: 523 1572 3351 Fragments arranged by size: 3351 1572 523 ApaLI G'TGCA_C Cuts at: 3503 4659 3503 Size: 1156 4290 ApoI r'AATT_y Cuts at: 1493 2755 2981 3133 5242 1493 Size: 1262 226 152 2109 1697 Fragments arranged by size: 2109 1697 1262 226 152 AseI AT'TA_AT Cuts at: 160 1032 4000 160 Size: 872 2968 1606 Fragments arranged by size: 2968 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5446 AvaI C'yCGr_G Cuts at: 1870 1943 2771 1870 Size: 73 828 4545 Fragments arranged by size: 4545 828 73 BamHI G'GATC_C Cuts at: 2448 2776 3143 2448 Size: 328 367 4751 Fragments arranged by size: 4751 367 328 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2767 4222 618 Size: 325 217 627 980 1455 1842 Fragments arranged by size: 1842 1455 980 627 325 217 BanII G_rGCy'C Cuts at: 1391 2682 2765 1391 Size: 1291 83 4072 Fragments arranged by size: 4072 1291 83 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2389 928 Size: 971 490 3985 Fragments arranged by size: 3985 971 490 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2020 2054 3637 3671 1903 Size: 34 83 34 1583 34 3678 Fragments arranged by size: 3678 1583 83 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3338 4548 3338 Size: 1210 4236 BclI T'GATC_A Cuts at: 1548 2070 1548 Size: 522 4924 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4057 136 Size: 107 122 71 631 605 337 2048 1525 Fragments arranged by size: 2048 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5441 5441 Size: 5446 BlpI GC'TnA_GC Cuts at: 2863 2863 Size: 5446 Bme1580I G_kGCm'C Cuts at: 1790 3507 4663 1790 Size: 1717 1156 2573 Fragments arranged by size: 2573 1717 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5446 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2575 3177 4135 Size: 416 53 83 399 24 1158 602 958 Cuts at: 4135 442 Size: 1753 Fragments arranged by size: 1753 1158 958 602 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5446 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2726 2726 Size: 5446 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2189 2721 4106 1239 Size: 950 532 1385 2579 Fragments arranged by size: 2579 1385 950 532 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3571 4436 4734 3571 Size: 865 298 4283 Fragments arranged by size: 4283 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2140 2375 4109 882 Size: 617 641 235 1734 2219 Fragments arranged by size: 2219 1734 641 617 235 BsaAI yAC'GTr Cuts at: 493 1204 3237 493 Size: 711 2033 2702 Fragments arranged by size: 2702 2033 711 BsaBI GATnn'nnATC Cuts at: 5440 5440 Size: 5446 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3253 3635 4270 275 Size: 53 83 186 936 1720 382 635 1451 Fragments arranged by size: 1720 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2187 3879 4277 4551 4698 5109 2187 Size: 1692 398 274 147 411 2524 Fragments arranged by size: 2524 1692 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5221 Fragments arranged by size: 5221 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2569 1186 Size: 1383 4063 BseYI C'CCAG_C Cuts at: 2081 4649 5145 2081 Size: 2568 496 2382 Fragments arranged by size: 2568 2382 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2014 1163 Size: 573 278 4595 Fragments arranged by size: 4595 573 278 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2188 2896 3657 4685 1540 Size: 275 186 187 708 761 1028 2301 Fragments arranged by size: 2301 1028 761 708 275 187 186 BsiHKAI G_wGCw'C Cuts at: 3507 3592 4663 3507 Size: 85 1071 4290 Fragments arranged by size: 4290 1071 85 BsmI GAATG_Cn' Cuts at: 2957 3050 2957 Size: 93 5353 BsmBI CGTCTCn'nnnn_ Cuts at: 2551 2551 Size: 5446 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2682 2765 3507 3592 4663 1391 Size: 399 892 83 742 85 1071 2174 Fragments arranged by size: 2174 1071 892 742 399 85 83 BspEI T'CCGG_A Cuts at: 5109 5109 Size: 5446 BspHI T'CATG_A Cuts at: 3335 5065 3335 Size: 1730 3716 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2801 844 Size: 1957 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3333 1394 Size: 429 1510 3507 Fragments arranged by size: 3507 1510 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3941 4115 65 Size: 1183 2693 174 1396 Fragments arranged by size: 2693 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2187 4090 4277 1700 Size: 110 377 1903 187 2869 Fragments arranged by size: 2869 1903 377 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5446 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5446 BssSI C'ACGA_G Cuts at: 3506 4518 3506 Size: 1012 4434 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5446 BstBI TT'CG_AA Cuts at: 4250 4250 Size: 5446 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5446 BstYI r'GATC_y Cuts at: 1972 2448 2776 3143 3528 3545 4997 5083 5095 Size: 476 328 367 385 17 1452 86 12 Cuts at: 5095 5441 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 476 385 367 346 328 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2452 2673 513 Size: 552 939 448 221 3286 Fragments arranged by size: 3286 939 552 448 221 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2295 487 Size: 683 56 1069 3638 Fragments arranged by size: 3638 1069 683 56 BtsI GCAGTG_nn' Cuts at: 2962 3756 3776 2962 Size: 794 20 4632 Fragments arranged by size: 4632 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5446 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5411 DraI TTT'AAA Cuts at: 2750 2750 Size: 5446 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2188 4453 809 Size: 507 872 2265 1802 Fragments arranged by size: 2265 1802 872 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2893 3782 5411 Size: 54 1425 50 275 18 1063 889 1629 Cuts at: 5411 8 Size: 43 Fragments arranged by size: 1629 1425 1063 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2893 1537 Size: 275 1081 4090 Fragments arranged by size: 4090 1081 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3376 1353 Size: 2023 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2336 4033 4407 4553 387 Size: 1064 138 747 1697 374 146 1280 Fragments arranged by size: 1697 1280 1064 747 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2189 2466 2721 3509 4106 4893 5161 Size: 393 557 277 255 788 597 787 268 Cuts at: 5161 1239 Size: 1524 Fragments arranged by size: 1524 788 787 597 557 393 277 268 255 EcoNI CCTnn'n_nnAGG Cuts at: 2249 2551 2249 Size: 302 5144 EcoO109I rG'GnC_Cy Cuts at: 2086 2706 2086 Size: 620 4826 EcoRI G'AATT_C Cuts at: 2755 3133 2755 Size: 378 5068 FspI TGC'GCA Cuts at: 3952 3952 Size: 5446 HaeII r_GCGC'y Cuts at: 1678 4593 1678 Size: 2915 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2315 2322 2347 2354 4087 Size: 32 135 32 743 7 25 7 1733 Cuts at: 4087 4119 4161 4193 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1733 743 135 42 32 32 32 32 25 7 7 HincII GTy'rAC Cuts at: 669 1657 1938 2790 3036 669 Size: 988 281 852 246 3079 Fragments arranged by size: 3079 988 852 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5446 HpaI GTT'AAC Cuts at: 3036 3036 Size: 5446 KpnI G_GTAC'C Cuts at: 2771 2771 Size: 5446 MfeI C'AATT_G Cuts at: 3045 3045 Size: 5446 MluI A'CGCG_T Cuts at: 5322 5322 Size: 5446 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4560 4744 1305 Size: 3255 184 2007 Fragments arranged by size: 3255 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3624 Fragments arranged by size: 3624 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3404 3763 3922 518 Size: 648 2238 359 159 2042 Fragments arranged by size: 2238 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2699 2862 3539 4687 4932 1606 Size: 1093 163 677 1148 245 2120 Fragments arranged by size: 2120 1148 1093 677 245 163 NcoI C'CATG_G Cuts at: 513 1065 2004 2452 2673 513 Size: 552 939 448 221 3286 Fragments arranged by size: 3286 939 552 448 221 NdeI CA'TA_TG Cuts at: 387 387 Size: 5446 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5446 NotI GC'GGCC_GC Cuts at: 2893 2893 Size: 5446 NspI r_CATG'y Cuts at: 1132 1229 2804 4349 1132 Size: 97 1575 1545 2229 Fragments arranged by size: 2229 1575 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5446 PciI A'CATG_T Cuts at: 4345 4345 Size: 5446 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5446 PmeI GTTT'AAAC Cuts at: 2750 2750 Size: 5446 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3515 3547 5028 5060 3515 Size: 32 1481 32 3901 Fragments arranged by size: 3901 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2086 2706 2086 Size: 620 4826 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5446 PsiI TTA'TAA Cuts at: 3016 3016 Size: 5446 PstI C_TGCA'G Cuts at: 830 2798 830 Size: 1968 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5446 PvuII CAG'CTG Cuts at: 2699 2699 Size: 5446 SalI G'TCGA_C Cuts at: 1936 2788 1936 Size: 852 4594 SanDI GG'GwC_CC Cuts at: 2086 2706 2086 Size: 620 4826 SbfI CC_TGCA'GG Cuts at: 2798 2798 Size: 5446 ScaI AGT'ACT Cuts at: 3694 5406 3694 Size: 1712 3734 SfcI C'TryA_G Cuts at: 826 1045 2249 2794 3929 4610 4801 826 Size: 219 1204 545 1135 681 191 1471 Fragments arranged by size: 1471 1204 1135 681 545 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5446 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5446 SmaI CCC'GGG Cuts at: 1872 2773 1872 Size: 901 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2285 3550 4451 4713 820 Size: 197 926 342 1265 901 262 1553 Fragments arranged by size: 1553 1265 926 901 342 262 197 SnaBI TAC'GTA Cuts at: 493 3237 493 Size: 2744 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5446 SphI G_CATG'C Cuts at: 1132 2804 1132 Size: 1672 3774 SspI AAT'ATT Cuts at: 5 52 2413 3370 5 Size: 47 2361 957 2081 Fragments arranged by size: 2361 2081 957 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5446 StyI C'CwwG_G Cuts at: 513 1065 2004 2452 2673 2885 513 Size: 552 939 448 221 212 3074 Fragments arranged by size: 3074 939 552 448 221 212 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2198 2350 3473 3490 3643 3828 4273 Size: 1255 618 152 1123 17 153 185 445 Cuts at: 4273 325 Size: 1498 Fragments arranged by size: 1498 1255 1123 618 445 185 153 152 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3692 5404 96 Size: 274 80 33 51 1443 1715 1712 138 Fragments arranged by size: 1715 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5406 5438 5406 Size: 32 5414 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5446 XbaI T'CTAG_A Cuts at: 2782 2782 Size: 5446 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2350 2350 Size: 5446 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5446 XmaI C'CCGG_G Cuts at: 1870 2771 1870 Size: 901 4545 XmnI GAAnn'nnTTC Cuts at: 3575 3575 Size: 5446 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3254 276 Size: 53 83 186 936 1720 2468 Fragments arranged by size: 2468 1720 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI