################################## # NO CUT ENZYMES ( 153 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AlwNI AocI Aor51HI ApaI AscI Asp700I Asp718I AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BclI BcnI BfrI BglI BlnI BpmI BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI EaeI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI FbaI FseI FspI HaeII HaeIII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MaeII MamI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI StuI SwaI Tth111I Van91I XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AfaI 259 GTAC GT^AC AhdI 72 GACACCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1915 GTGCAC G^TGCAC ApaLI 1915 GTGCAC G^TGCAC AseI 376 ATTAAT AT^TAAT AsnI 376 ATTAAT AT^TAAT AspEI 72 GACACCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BanI 1882 GGCACC G^G[CT][GA]CC BbsI 1152 GTCTTC GAAGAC(2/6) BglII 456 AGATCT A^GATCT BlpI 1689 GCTTAGC GC^T[ACGT]AGC Bpu1102I 1689 GCTTAGC GC^T[ACGT]AGC BpuAI 1152 GTCTTC GAAGAC(2/6) BsaI 1186 GAGACC GGTCTC(1/5) BsmI 333 GAATGC GAATGC(1/-1) BsrBI 13 CCGCTC CCGCTC(-3/-3) CelII 1689 GCTTAGC GC^T[ACGT]AGC DsaI 1625 CCGTGG C^C[GA][CT]GG Eam1105I 72 GACACCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRV 705 GATATC GAT^ATC EcoT14I 545 CCAAGG C^C[AT][AT]GG EcoT22I 1332 ATGCAT ATGCA^T FauI 12 CCCGC CCCGC(4/6) HapII 1481 CCGG C^CGG HpaII 1481 CCGG C^CGG MspI 1481 CCGG C^CGG NsiI 1332 ATGCAT ATGCA^T NspI 1732 GCATGC [GA]CATG^[CT] PshBI 376 ATTAAT AT^TAAT PstI 1799 CTGCAG CTGCA^G PvuII 1146 CAGCTG CAG^CTG RsaI 259 GTAC GT^AC SacI 1020 GAGCTC GAGCT^C SfaNI 1921 GCATC GCATC(5/9) SphI 1732 GCATGC GCATG^C StyI 545 CCAAGG C^C[AT][AT]GG TaqI 1077 TCGA T^CGA TthHB8I 1077 TCGA T^CGA XbaI 2002 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AciI 13 CCGC CCGC(-3/-1) 1203 CCGC AspHI 1020 GAGCTC G[AT]GC[AT]^C 1915 GTGCAC BanII 1020 GAGCTC G[GA]GC[CT]^C 1856 GAGCCC BciVI 612 GGATAC GTATCC(6/5) 1654 GGATAC BseRI 979 GAGGAG GAGGAG(10/8) 1853 GAGGAG BsiHKAI 1020 GAGCTC G[AT]GC[AT]^C 1915 GTGCAC BsiYI 1249 CCTTCCATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1620 CCCAGCCGTGG BslI 1249 CCTTCCATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1620 CCCAGCCGTGG BsmFI 629 GGGAC GGGAC(10/14) 2044 GTCCC MslI 1517 CATGGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1551 CATTGTTGTG MspA1I 1146 CAGCTG C[AC]G^C[GT]G 1203 CCGCTG SapI 1461 GCTCTTC GCTCTTC(1/4) 1864 GCTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 54 enzymes) # ######################################### AcsI AluI AlwI ApoI AvaII BbvI BfaI BmyI BsaJI BsmAI Bsp1286I BsrDI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EarI EcoRII Fnu4HI FokI HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MvaI MwoI NdeI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfcI SspI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII