(Circular) (Six-base) MAPSORT of: pFN21AE9422.seq Check: 69 from: 1 to: 6775 pFN21AE9422 6775 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4585 278 Size: 53 83 186 936 3049 2468 Fragments arranged by size: 3049 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4118 1937 Size: 2181 4594 Acc65I G'GTAC_C Cuts at: 4096 4096 Size: 6775 AclI AA'CG_TT Cuts at: 1563 4902 5275 1563 Size: 3339 373 3063 Fragments arranged by size: 3339 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2257 2988 3000 3144 3343 4838 6222 6490 Size: 625 731 12 144 199 1495 1384 268 Cuts at: 6490 1632 Size: 1917 Fragments arranged by size: 1917 1495 1384 731 625 268 199 144 12 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6578 AflIII A'CryG_T Cuts at: 1203 5674 6651 1203 Size: 4471 977 1327 Fragments arranged by size: 4471 1327 977 AgeI A'CCGG_T Cuts at: 5606 5606 Size: 6775 AhdI GACnn_n'nnGTC Cuts at: 2082 5504 2082 Size: 3422 3353 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6775 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3544 1184 Size: 2360 4415 AlwNI CAG_nnn'CTG Cuts at: 4518 6090 4518 Size: 1572 5203 ApaLI G'TGCA_C Cuts at: 3920 4832 5988 3920 Size: 912 1156 4707 Fragments arranged by size: 4707 1156 912 ApoI r'AATT_y Cuts at: 1493 2194 2449 2582 2749 3366 3598 4084 4310 Size: 701 255 133 167 617 232 486 226 Cuts at: 4310 4462 6571 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 701 617 486 255 232 226 167 152 133 AseI AT'TA_AT Cuts at: 160 1032 2382 5329 160 Size: 872 1350 2947 1606 Fragments arranged by size: 2947 1606 1350 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6775 AvaI C'yCGr_G Cuts at: 1870 1943 4100 1870 Size: 73 2157 4545 Fragments arranged by size: 4545 2157 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2610 2643 2610 Size: 33 6742 BamHI G'GATC_C Cuts at: 4105 4472 4105 Size: 367 6408 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3887 4096 5551 618 Size: 325 217 627 2100 209 1455 1842 Fragments arranged by size: 2100 1842 1455 627 325 217 209 BanII G_rGCy'C Cuts at: 1391 3029 3865 4094 1391 Size: 1638 836 229 4072 Fragments arranged by size: 4072 1638 836 229 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3150 928 Size: 971 1251 4553 Fragments arranged by size: 4553 1251 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4966 5000 1903 Size: 34 3029 34 3678 Fragments arranged by size: 3678 3029 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2611 3653 4667 5877 2611 Size: 1042 1014 1210 3509 Fragments arranged by size: 3509 1210 1042 1014 BclI T'GATC_A Cuts at: 1548 1548 Size: 6775 BfrBI ATG'CAT Cuts at: 3339 3339 Size: 6775 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5386 136 Size: 107 122 71 631 605 3714 1525 Fragments arranged by size: 3714 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2461 6770 2461 Size: 4309 2466 BlpI GC'TnA_GC Cuts at: 3695 4192 3695 Size: 497 6278 Bme1580I G_kGCm'C Cuts at: 1790 3924 4836 5992 1790 Size: 2134 912 1156 2573 Fragments arranged by size: 2573 2134 1156 912 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6775 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4506 5464 442 Size: 416 53 83 399 24 3089 958 1753 Fragments arranged by size: 3089 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6775 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3881 3881 Size: 6775 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5435 1239 Size: 4196 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4900 5765 6063 4900 Size: 865 298 5612 Fragments arranged by size: 5612 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3185 5438 882 Size: 617 1686 2253 2219 Fragments arranged by size: 2253 2219 1686 617 BsaAI yAC'GTr Cuts at: 493 1204 4566 493 Size: 711 3362 2702 Fragments arranged by size: 3362 2702 711 BsaBI GATnn'nnATC Cuts at: 6769 6769 Size: 6775 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4582 4964 5599 275 Size: 53 83 186 936 3049 382 635 1451 Fragments arranged by size: 3049 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5208 5606 5880 6027 6438 5208 Size: 398 274 147 411 5545 Fragments arranged by size: 5545 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2070 2100 3097 3127 1178 Size: 30 165 30 667 30 997 30 4826 Fragments arranged by size: 4826 997 667 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2999 3873 1186 Size: 1813 874 4088 Fragments arranged by size: 4088 1813 874 BseYI C'CCAG_C Cuts at: 3625 5978 6474 3625 Size: 2353 496 3926 Fragments arranged by size: 3926 2353 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6202 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4225 4986 6014 1540 Size: 275 186 2224 761 1028 2301 Fragments arranged by size: 2301 2224 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3029 3924 4836 4921 5992 3029 Size: 895 912 85 1071 3812 Fragments arranged by size: 3812 1071 912 895 85 BsmI GAATG_Cn' Cuts at: 2344 4286 4379 2344 Size: 1942 93 4740 Fragments arranged by size: 4740 1942 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3029 3865 3924 4094 4836 4921 5992 Size: 399 1239 836 59 170 742 85 1071 Cuts at: 5992 1391 Size: 2174 Fragments arranged by size: 2174 1239 1071 836 742 399 170 85 59 BspEI T'CCGG_A Cuts at: 6438 6438 Size: 6775 BspHI T'CATG_A Cuts at: 4664 6394 4664 Size: 1730 5045 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4130 844 Size: 3286 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2020 4662 1394 Size: 429 197 2642 3507 Fragments arranged by size: 3507 2642 429 197 BsrDI GCAATG_nn' Cuts at: 65 1248 2119 2890 3480 3967 5270 5444 65 Size: 1183 871 771 590 487 1303 174 1396 Fragments arranged by size: 1396 1303 1183 871 771 590 487 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5419 5606 1700 Size: 110 3609 187 2869 Fragments arranged by size: 3609 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6775 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6775 BssSI C'ACGA_G Cuts at: 4835 5847 4835 Size: 1012 5763 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6775 BstBI TT'CG_AA Cuts at: 5579 5579 Size: 6775 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6775 BstYI r'GATC_y Cuts at: 1972 2276 2405 2461 3897 4105 4472 4857 4874 Size: 304 129 56 1436 208 367 385 17 Cuts at: 4874 6326 6412 6424 6770 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1436 385 367 346 304 208 129 86 56 17 12 BtgI C'CryG_G Cuts at: 513 1065 3630 513 Size: 552 2565 3658 Fragments arranged by size: 3658 2565 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6036 Fragments arranged by size: 6036 683 56 BtsI GCAGTG_nn' Cuts at: 3801 4291 5085 5105 3801 Size: 490 794 20 5471 Fragments arranged by size: 5471 794 490 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6775 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2722 2757 529 Size: 35 2158 35 4547 Fragments arranged by size: 4547 2158 35 35 DraI TTT'AAA Cuts at: 4079 4079 Size: 6775 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5782 809 Size: 507 4466 1802 Fragments arranged by size: 4466 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4222 5111 6740 Size: 54 1425 50 275 18 2392 889 1629 Cuts at: 6740 8 Size: 43 Fragments arranged by size: 2392 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4222 1537 Size: 275 2410 4090 Fragments arranged by size: 4090 2410 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2976 3473 3876 3953 4705 1353 Size: 1623 497 403 77 752 3423 Fragments arranged by size: 3423 1623 752 497 403 77 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5362 5736 5882 387 Size: 1064 138 3773 374 146 1280 Fragments arranged by size: 3773 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3027 3027 Size: 6775 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2257 2988 3000 3144 3343 4838 5435 Size: 393 625 731 12 144 199 1495 597 Cuts at: 5435 6222 6490 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1495 787 731 625 597 393 268 199 144 12 EcoRI G'AATT_C Cuts at: 4084 4462 4084 Size: 378 6397 EcoRV GAT'ATC Cuts at: 2712 2712 Size: 6775 FspI TGC'GCA Cuts at: 5281 5281 Size: 6775 HaeII r_GCGC'y Cuts at: 1678 5922 1678 Size: 4244 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2068 2100 2627 2650 2659 Size: 32 135 32 496 32 527 23 9 Cuts at: 2659 2682 2905 2937 5416 5448 5490 5522 1373 Size: 23 223 32 2479 32 42 32 2626 Fragments arranged by size: 2626 2479 527 496 223 135 42 32 32 32 32 32 32 23 23 9 HincII GTy'rAC Cuts at: 669 1657 1938 4119 4365 669 Size: 988 281 2181 246 3079 Fragments arranged by size: 3079 2181 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6775 HpaI GTT'AAC Cuts at: 4365 4365 Size: 6775 KpnI G_GTAC'C Cuts at: 4100 4100 Size: 6775 MfeI C'AATT_G Cuts at: 4374 4374 Size: 6775 MluI A'CGCG_T Cuts at: 6651 6651 Size: 6775 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3619 5889 6073 1305 Size: 2314 2270 184 2007 Fragments arranged by size: 2314 2270 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4953 Fragments arranged by size: 4953 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3526 3560 4733 5092 5251 518 Size: 648 2360 34 1173 359 159 2042 Fragments arranged by size: 2360 2042 1173 648 359 159 34 MspA1I CmG'CkG Cuts at: 1606 3153 3210 4191 4868 6016 6261 1606 Size: 1547 57 981 677 1148 245 2120 Fragments arranged by size: 2120 1547 1148 981 677 245 57 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6223 NdeI CA'TA_TG Cuts at: 387 2817 2869 3335 387 Size: 2430 52 466 3827 Fragments arranged by size: 3827 2430 466 52 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6775 NotI GC'GGCC_GC Cuts at: 4222 4222 Size: 6775 NsiI A_TGCA'T Cuts at: 3341 3341 Size: 6775 NspI r_CATG'y Cuts at: 1132 1229 3741 4133 5678 1132 Size: 97 2512 392 1545 2229 Fragments arranged by size: 2512 2229 1545 392 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6775 PciI A'CATG_T Cuts at: 5674 5674 Size: 6775 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6775 PmeI GTTT'AAAC Cuts at: 4079 4079 Size: 6775 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4844 4876 6357 6389 4844 Size: 32 1481 32 5230 Fragments arranged by size: 5230 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6775 PsiI TTA'TAA Cuts at: 2910 3276 4345 2910 Size: 366 1069 5340 Fragments arranged by size: 5340 1069 366 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2653 2685 2653 Size: 32 6743 PstI C_TGCA'G Cuts at: 830 3808 4127 830 Size: 2978 319 3478 Fragments arranged by size: 3478 2978 319 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6775 PvuII CAG'CTG Cuts at: 3153 3153 Size: 6775 SacI G_AGCT'C Cuts at: 3029 3029 Size: 6775 SalI G'TCGA_C Cuts at: 1936 4117 1936 Size: 2181 4594 SapI GCTCTTCn'nnn_ Cuts at: 3473 3876 3473 Size: 403 6372 SbfI CC_TGCA'GG Cuts at: 4127 4127 Size: 6775 ScaI AGT'ACT Cuts at: 5023 6735 5023 Size: 1712 5063 SfcI C'TryA_G Cuts at: 826 1045 2443 3406 3804 4123 5258 5939 6130 Size: 219 1398 963 398 319 1135 681 191 Cuts at: 6130 826 Size: 1471 Fragments arranged by size: 1471 1398 1135 963 681 398 319 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6775 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6775 SmaI CCC'GGG Cuts at: 1872 4102 1872 Size: 2230 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4879 5780 6042 820 Size: 197 926 2936 901 262 1553 Fragments arranged by size: 2936 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4566 493 Size: 4073 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6775 SphI G_CATG'C Cuts at: 1132 3741 4133 1132 Size: 2609 392 3774 Fragments arranged by size: 3774 2609 392 SspI AAT'ATT Cuts at: 5 52 2380 2499 3280 4028 4699 5 Size: 47 2328 119 781 748 671 2081 Fragments arranged by size: 2328 2081 781 748 671 119 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6775 StyI C'CwwG_G Cuts at: 513 1065 2550 4214 513 Size: 552 1485 1664 3074 Fragments arranged by size: 3074 1664 1485 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3052 3554 4802 4819 4972 5157 5602 Size: 1255 1472 502 1248 17 153 185 445 Cuts at: 5602 325 Size: 1498 Fragments arranged by size: 1498 1472 1255 1248 502 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5021 6733 96 Size: 274 80 33 51 1443 3044 1712 138 Fragments arranged by size: 3044 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2070 2102 3097 3129 6735 6767 2070 Size: 32 995 32 3606 32 2078 Fragments arranged by size: 3606 2078 995 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6775 XbaI T'CTAG_A Cuts at: 4007 4111 4007 Size: 104 6671 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6775 XmaI C'CCGG_G Cuts at: 1870 4100 1870 Size: 2230 4545 XmnI GAAnn'nnTTC Cuts at: 4904 4904 Size: 6775 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4583 276 Size: 53 83 186 936 3049 2468 Fragments arranged by size: 3049 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI