################################## # NO CUT ENZYMES ( 156 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BamHI BbrPI BbsI BcgI BciVI BfaI BfrI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FseI FspI HgaI HincII HindII HindIII HpaI KpnI Ksp632I MaeI MamI MboII MfeI MflI MluI MroI MseI MslI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tru9I Tsp509I Tth111I TthHB8I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccII 229 CGCG CG^CG AcyI 194 GGCGCC G[GA]^CG[CT]C AfaI 8 GTAC GT^AC ApaI 202 GGGCCC GGGCC^C AvaI 296 CCCGGG C^[CT]CG[GA]G BalI 68 TGGCCA TGG^CCA BbeI 194 GGCGCC GGCGC^C BbvCI 285 CCTCAGC CCTCAGC(-5/-2) BclI 125 TGATCA T^GATCA BglI 352 GCCACCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaHI 194 GGCGCC G[GA]^CG[CT]C BsaI 409 GAGACC GGTCTC(1/5) BsmBI 103 GAGACG CGTCTC(1/5) BsoBI 296 CCCGGG C^[CT]CG[GA]G BspHI 330 TCATGA T^CATGA BsrBI 375 CCGCTC CCGCTC(-3/-3) BstEII 364 GGTCACC G^GT[ACGT]ACC BstF5I 416 GGATG GGATG(2/0) BstPI 364 GGTCACC G^GT[ACGT]ACC BstUI 229 CGCG CG^CG DpnII 126 GATC ^GATC EaeI 68 TGGCCA [CT]^GGCC[GA] EcoO65I 364 GGTCACC G^GT[ACGT]ACC FbaI 125 TGATCA T^GATCA FokI 416 GGATG GGATG(9/13) Hin1I 194 GGCGCC G[GA]^CG[CT]C KasI 194 GGCGCC G^GCGCC KspI 228 CCGCGG CCGC^GG MaeII 160 ACGT A^CGT MaeIII 365 GTCAC ^GT[ACGT]AC MboI 126 GATC ^GATC MluNI 68 TGGCCA TGG^CCA MscI 68 TGGCCA TGG^CCA MvnI 229 CGCG CG^CG NarI 194 GGCGCC GG^CGCC NdeII 126 GATC ^GATC PleI 463 GAGTC GAGTC(4/5) PvuII 121 CAGCTG CAG^CTG RcaI 330 TCATGA T^CATGA RsaI 8 GTAC GT^AC SacII 228 CCGCGG CCGC^GG Sau3AI 126 GATC ^GATC SfaNI 347 GCATC GCATC(5/9) SfoI 194 GGCGCC GGC^GCC SmaI 296 CCCGGG CCC^GGG TfiI 319 GATTC G^A[AT]TC Tsp45I 365 GTCAC ^GT[GC]AC XmaCI 296 CCCGGG C^CCGGG XmaI 296 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### BanI 194 GGCGCC G^G[CT][GA]CC 212 GGTGCC BanII 136 GAGCCC G[GA]GC[CT]^C 202 GGGCCC BsmAI 103 GAGAC GTCTC(1/5) 409 GAGAC BsmFI 233 GGGAC GGGAC(10/14) 162 GTCCC BspMI 209 GCAGGT ACCTGC(4/8) 270 GCAGGT DraII 202 GGGCCCC [GA]G^G[ACGT]CC[CT] 281 AGGGCCT EcoO109I 202 GGGCCCC [GA]G^G[ACGT]CC[CT] 281 AGGGCCT EcoT14I 83 CCATGG C^C[AT][AT]GG 198 CCATGG HaeII 194 GGCGCC [GA]GCGC^[CT] 386 AGCGCC HinfI 319 GATTC G^A[ACGT]TC 463 GAGTC MspA1I 121 CAGCTG C[AC]G^C[GT]G 228 CCGCGG NcoI 83 CCATGG C^CATGG 198 CCATGG PflMI 44 CCATTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 60 CCATCTAATGG StyI 83 CCATGG C^C[AT][AT]GG 198 CCATGG Van91I 44 CCATTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 60 CCATCTAATGG ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AciI AluI AvaII BbvI BcnI BmyI BsaJI BsiYI BslI Bsp1286I BssKI BstNI Cac8I CfoI Cfr13I DdeI DsaI EcoRII FauI Fnu4HI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MnlI MspI MvaI MwoI NciI NlaIII NlaIV Sau96I ScrFI TseI TspRI