Restriction map for
pFN21AE6563
(Circular) (Six-base) MAPSORT of: pFN21AE6563.seq Check: 2996 from: 1 to: 5230
pFN21AE6563 5230 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2205 2205
Size: 5230
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3040 278
Size: 53 83 186 936 1504 2468
Fragments arranged by size:
2468 1504 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2573 1937
Size: 636 4594
Acc65I G'GTAC_C
Cuts at: 2551 2551
Size: 5230
AclI AA'CG_TT
Cuts at: 1563 3357 3730 1563
Size: 1794 373 3063
Fragments arranged by size:
3063 1794 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2291 3293 4677 4945 1632
Size: 659 1002 1384 268 1917
Fragments arranged by size:
1917 1384 1002 659 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5033
AflIII A'CryG_T
Cuts at: 1203 4129 5106 1203
Size: 2926 977 1327
Fragments arranged by size:
2926 1327 977
AgeI A'CCGG_T
Cuts at: 4061 4061
Size: 5230
AhdI GACnn_n'nnGTC
Cuts at: 3959 3959
Size: 5230
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5230
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5230
AlwNI CAG_nnn'CTG
Cuts at: 2973 4545 2973
Size: 1572 3658
ApaI G_GGCC'C
Cuts at: 2211 2211
Size: 5230
ApaLI G'TGCA_C
Cuts at: 3287 4443 3287
Size: 1156 4074
ApoI r'AATT_y
Cuts at: 1493 2539 2765 2917 5026 1493
Size: 1046 226 152 2109 1697
Fragments arranged by size:
2109 1697 1046 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3784 160
Size: 872 2752 1606
Fragments arranged by size:
2752 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5230
AvaI C'yCGr_G
Cuts at: 1870 1943 2301 2555 1870
Size: 73 358 254 4545
Fragments arranged by size:
4545 358 254 73
BamHI G'GATC_C
Cuts at: 2560 2927 2560
Size: 367 4863
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2199 2217 2551 4006 618
Size: 325 217 627 412 18 334 1455 1842
Fragments arranged by size:
1842 1455 627 412 334 325 217 18
BanII G_rGCy'C
Cuts at: 1391 2145 2211 2549 1391
Size: 754 66 338 4072
Fragments arranged by size:
4072 754 338 66
BbeI G_GCGC'C
Cuts at: 2203 2203
Size: 5230
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4259
BbvCI CC'TCA_GC
Cuts at: 2291 2291
Size: 5230
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3421 3455 1903
Size: 34 50 34 1400 34 3678
Fragments arranged by size:
3678 1400 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3122 4332 3122
Size: 1210 4020
BclI T'GATC_A
Cuts at: 1548 2130 1548
Size: 582 4648
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2363 3841 136
Size: 107 122 71 631 605 691 1478 1525
Fragments arranged by size:
1525 1478 691 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5225 5225
Size: 5230
BlpI GC'TnA_GC
Cuts at: 2647 2647
Size: 5230
Bme1580I G_kGCm'C
Cuts at: 1790 2025 2211 3291 4447 1790
Size: 235 186 1080 1156 2573
Fragments arranged by size:
2573 1156 1080 235 186
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5230
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2961 3919 442
Size: 416 53 83 399 24 1544 958 1753
Fragments arranged by size:
1753 1544 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5230
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2131 2173 2131
Size: 42 5188
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3890 1239
Size: 2651 2579
Bpu10I CC'TnA_GC
Cuts at: 2291 2291
Size: 5230
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3355 4220 4518 3355
Size: 865 298 4067
Fragments arranged by size:
4067 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2408 3893 882
Size: 617 909 1485 2219
Fragments arranged by size:
2219 1485 909 617
BsaAI yAC'GTr
Cuts at: 493 1204 3021 493
Size: 711 1817 2702
Fragments arranged by size:
2702 1817 711
BsaBI GATnn'nnATC
Cuts at: 5224 5224
Size: 5230
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2200 3037 3419 4054
Size: 53 83 186 936 667 837 382 635
Cuts at: 4054 275
Size: 1451
Fragments arranged by size:
1451 936 837 667 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3663 4061 4335 4482 4893 3663
Size: 398 274 147 411 4000
Fragments arranged by size:
4000 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5005
Fragments arranged by size:
5005 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5230
BseYI C'CCAG_C
Cuts at: 4433 4929 4433
Size: 496 4734
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4657
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2680 3441 4469 1540
Size: 275 186 679 761 1028 2301
Fragments arranged by size:
2301 1028 761 679 275 186
BsiHKAI G_wGCw'C
Cuts at: 3291 3376 4447 3291
Size: 85 1071 4074
Fragments arranged by size:
4074 1071 85
BsmI GAATG_Cn'
Cuts at: 2741 2834 2741
Size: 93 5137
BsmBI CGTCTCn'nnnn_
Cuts at: 2102 2102
Size: 5230
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2025 2145 2211 2549 3291 3376 4447
Size: 399 235 120 66 338 742 85 1071
Cuts at: 4447 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 338 235 120 85
66
BspEI T'CCGG_A
Cuts at: 4893 4893
Size: 5230
BspHI T'CATG_A
Cuts at: 2335 3119 4849 2335
Size: 784 1730 2716
Fragments arranged by size:
2716 1730 784
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2205 2266 2585 844
Size: 1361 61 319 3489
Fragments arranged by size:
3489 1361 319 61
BsrBI CCG'CTC
Cuts at: 1394 1823 2382 3117 1394
Size: 429 559 735 3507
Fragments arranged by size:
3507 735 559 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3725 3899 65
Size: 1183 2477 174 1396
Fragments arranged by size:
2477 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3874 4061 1700
Size: 110 2064 187 2869
Fragments arranged by size:
2869 2064 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5230
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5230
BssSI C'ACGA_G
Cuts at: 3290 4302 3290
Size: 1012 4218
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5230
BstBI TT'CG_AA
Cuts at: 4034 4034
Size: 5230
BstEII G'GTnAC_C
Cuts at: 1184 2369 1184
Size: 1185 4045
BstYI r'GATC_y
Cuts at: 1972 2560 2927 3312 3329 4781 4867 4879 5225
Size: 588 367 385 17 1452 86 12 346
Cuts at: 5225 1972
Size: 1977
Fragments arranged by size:
1977 1452 588 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2088 2203 2233 513
Size: 552 1023 115 30 3510
Fragments arranged by size:
3510 1023 552 115 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2338 487
Size: 683 56 1112 3379
Fragments arranged by size:
3379 1112 683 56
BtsI GCAGTG_nn'
Cuts at: 2076 2403 2746 3540 3560 2076
Size: 327 343 794 20 3746
Fragments arranged by size:
3746 794 343 327 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5230
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5195
DraI TTT'AAA
Cuts at: 2534 2534
Size: 5230
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4237 809
Size: 507 2921 1802
Fragments arranged by size:
2921 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2073 2677 3566
Size: 54 1425 50 275 18 243 604 889
Cuts at: 3566 5195 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 604 275 243 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2677 1537
Size: 275 865 4090
Fragments arranged by size:
4090 865 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3160 1353
Size: 1807 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2074 2249 3817 4191 4337 387
Size: 1064 138 485 175 1568 374 146 1280
Fragments arranged by size:
1568 1280 1064 485 374 175 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2291 3293 3890 4677 4945 1239
Size: 393 659 1002 597 787 268 1524
Fragments arranged by size:
1524 1002 787 659 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2208 2287 2208
Size: 79 5151
EcoRI G'AATT_C
Cuts at: 2539 2917 2539
Size: 378 4852
FspI TGC'GCA
Cuts at: 3736 3736
Size: 5230
HaeII r_GCGC'y
Cuts at: 1678 2203 2395 4377 1678
Size: 525 192 1982 2531
Fragments arranged by size:
2531 1982 525 192
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3871 3903 3945 3977 1373
Size: 32 135 32 2299 32 42 32 2626
Fragments arranged by size:
2626 2299 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2574 2820 669
Size: 988 281 636 246 3079
Fragments arranged by size:
3079 988 636 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5230
HpaI GTT'AAC
Cuts at: 2820 2820
Size: 5230
KasI G'GCGC_C
Cuts at: 2199 2199
Size: 5230
KpnI G_GTAC'C
Cuts at: 2555 2555
Size: 5230
MfeI C'AATT_G
Cuts at: 2829 2829
Size: 5230
MluI A'CGCG_T
Cuts at: 5106 5106
Size: 5230
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2250 4344 4528 1305
Size: 945 2094 184 2007
Fragments arranged by size:
2094 2007 945 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2075 10
Size: 54 1425 343 243 3165
Fragments arranged by size:
3165 1425 343 243 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3188 3547 3706 518
Size: 648 2022 359 159 2042
Fragments arranged by size:
2042 2022 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2128 2235 2646 3323 4471 4716 1606
Size: 522 107 411 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 522 411 245 107
NarI GG'CG_CC
Cuts at: 2200 2200
Size: 5230
NcoI C'CATG_G
Cuts at: 513 1065 2088 2203 513
Size: 552 1023 115 3540
Fragments arranged by size:
3540 1023 552 115
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5230
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5230
NotI GC'GGCC_GC
Cuts at: 2677 2677
Size: 5230
NspI r_CATG'y
Cuts at: 1132 1229 2588 4133 1132
Size: 97 1359 1545 2229
Fragments arranged by size:
2229 1545 1359 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5230
PciI A'CATG_T
Cuts at: 4129 4129
Size: 5230
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2055 2071 1108
Size: 947 16 4267
Fragments arranged by size:
4267 947 16
PmeI GTTT'AAAC
Cuts at: 2534 2534
Size: 5230
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3299 3331 4812 4844 3299
Size: 32 1481 32 3685
Fragments arranged by size:
3685 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5230
PsiI TTA'TAA
Cuts at: 2800 2800
Size: 5230
PspOMI G'GGCC_C
Cuts at: 2207 2207
Size: 5230
PstI C_TGCA'G
Cuts at: 830 2582 830
Size: 1752 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5230
PvuII CAG'CTG
Cuts at: 2128 2128
Size: 5230
SacII CC_GC'GG
Cuts at: 2236 2236
Size: 5230
SalI G'TCGA_C
Cuts at: 1936 2572 1936
Size: 636 4594
SbfI CC_TGCA'GG
Cuts at: 2582 2582
Size: 5230
ScaI AGT'ACT
Cuts at: 3478 5190 3478
Size: 1712 3518
SfcI C'TryA_G
Cuts at: 826 1045 2578 3713 4394 4585 826
Size: 219 1533 1135 681 191 1471
Fragments arranged by size:
1533 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2201 2201
Size: 5230
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5230
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5230
SmaI CCC'GGG
Cuts at: 1872 2303 2557 1872
Size: 431 254 4545
Fragments arranged by size:
4545 431 254
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3334 4235 4497 820
Size: 197 926 1391 901 262 1553
Fragments arranged by size:
1553 1391 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3021 493
Size: 2528 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5230
SphI G_CATG'C
Cuts at: 1132 2588 1132
Size: 1456 3774
SspI AAT'ATT
Cuts at: 5 52 3154 5
Size: 47 3102 2081
Fragments arranged by size:
3102 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5230
StyI C'CwwG_G
Cuts at: 513 1065 2088 2203 2669 513
Size: 552 1023 115 466 3074
Fragments arranged by size:
3074 1023 552 466 115
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2150 3257 3274 3427 3612 4057 325
Size: 1255 570 1107 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1107 570 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3476 5188 96
Size: 274 80 33 51 1443 1499 1712 138
Fragments arranged by size:
1712 1499 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5190 5222 5190
Size: 32 5198
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5230
XbaI T'CTAG_A
Cuts at: 2566 2566
Size: 5230
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5230
XmaI C'CCGG_G
Cuts at: 1870 2301 2555 1870
Size: 431 254 4545
Fragments arranged by size:
4545 431 254
XmnI GAAnn'nnTTC
Cuts at: 3359 3359
Size: 5230
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3038 276
Size: 53 83 186 936 1504 2468
Fragments arranged by size:
2468 1504 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstXI
BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspXI PsrI RsrII SacI SanDI SapI SexAI
SfiI SrfI SwaI XcmI