Restriction map for
pFN21AE2413
##################################
# NO CUT ENZYMES ( 47 enzymes) #
##################################
AclI AflII Aor51HI AscI AseI
AsnI BfrI BlpI Bpu1102I BsiEI
BsiWI BspLU11I BssHII BstBI CelII
CpoI DraI DrdI EagI EclXI
Eco47III Eco52I EcoRV FseI HpaI
MfeI MluI MunI NaeI NgoMIV
NheI NotI NruI NspV PacI
PmeI PshBI Psp1406I PvuI RsrII
SalI SfuI SgrAI SpeI SspI
SwaI XbaI
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# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 1346 GACGTC GACGT^C
AgeI 1423 ACCGGT A^CCGGT
AhdI 5860 GACGAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 873 GTGCAC G^TGCAC
ApaLI 873 GTGCAC G^TGCAC
AspEI 5860 GACGAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3208 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 5615 TGCGCA TGC^GCA
AvrII 132 CCTAGG C^CTAGG
BbeI 1840 GGCGCC GGCGC^C
BbrPI 5635 CACGTG CAC^GTG
BciVI 4463 GTATCC GTATCC(6/5)
BlnI 132 CCTAGG C^CTAGG
Bsp1407I 1483 TGTACA T^GTACA
BspDI 3286 ATCGAT AT^CGAT
BsrBI 3175 GAGCGG CCGCTC(-3/-3)
BsrFI 1423 ACCGGT [GA]^CCGG[CT]
BsrGI 1483 TGTACA T^GTACA
BssSI 727 CTCGTG CACGAG(-5/-1)
Bst1107I 3136 GTATAC GTA^TAC
BstZ17I 3136 GTATAC GTA^TAC
Cfr10I 1423 ACCGGT [GA]^CCGG[CT]
ClaI 3286 ATCGAT AT^CGAT
Eam1105I 5860 GACGAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 378 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 4381 GAATTC G^AATTC
EcoT22I 3290 ATGCAT ATGCA^T
FspI 5615 TGCGCA TGC^GCA
HindIII 3913 AAGCTT A^AGCTT
KasI 1840 GGCGCC G^GCGCC
NarI 1840 GGCGCC GG^CGCC
NsiI 3290 ATGCAT ATGCA^T
PflFI 3208 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1423 ACCGGT A^CCGGT
PmaCI 5635 CACGTG CAC^GTG
PmlI 5635 CACGTG CAC^GTG
PshAI 4876 GACTTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI 5351 ACCTGGT A^CC[AT]GGT
SfiI 1294 GGCCGGGCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1840 GGCGCC GGC^GCC
SmaI 88 CCCGGG CCC^GGG
SnaBI 394 TACGTA TAC^GTA
SspBI 1483 TGTACA T^GTACA
Tth111I 3208 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 88 CCCGGG C^CCGGG
XmaI 88 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 3136 GTATAC GT^[AC][GT]AC
3355 GTCTAC
AccIII 2990 TCCGGA T^CCGGA
4592 TCCGGA
Asp700I 273 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3387 GAACATCTTC
BclI 4292 TGATCA T^GATCA
4316 TGATCA
BglII 3335 AGATCT A^GATCT
4667 AGATCT
BsaAI 394 TACGTA [CT]AC^GT[GA]
5635 CACGTG
BsaBI 3384 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4653 GATTGTCATC
BseAI 2990 TCCGGA T^CCGGA
4592 TCCGGA
BsmI 785 GAATGC GAATGC(1/-1)
3115 GAATGC
BspEI 2990 TCCGGA T^CCGGA
4592 TCCGGA
DraIII 670 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG
4501 CACCGAGTG
FbaI 4292 TGATCA T^GATCA
4316 TGATCA
HincII 1629 GTTGAC GT[CT]^[GA]AC
3187 GTCAAC
HindII 1629 GTTGAC GT[CT]^[GA]AC
3187 GTCAAC
KspI 897 CCGCGG CCGC^GG
2793 CCGCGG
MamI 3384 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4653 GATTGTCATC
MroI 2990 TCCGGA T^CCGGA
4592 TCCGGA
MseI 1898 TTAA T^TAA
2819 TTAA
NdeI 2168 CATATG CA^TATG
3293 CATATG
PaeR7I 766 CTCGAG C^TCGAG
5735 CTCGAG
SacI 3846 GAGCTC GAGCT^C
5327 GAGCTC
SacII 897 CCGCGG CCGC^GG
2793 CCGCGG
ScaI 2907 AGTACT AGT^ACT
2916 AGTACT
SphI 2021 GCATGC GCATG^C
4034 GCATGC
Tru9I 1898 TTAA T^TAA
2819 TTAA
XhoI 766 CTCGAG C^TCGAG
5735 CTCGAG
XmnI 273 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3387 GAACATCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 138 enzymes) #
#########################################
Acc65I AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
AocI ApaI ApoI Asp718I AspHI
AvaI AvaII BalI BamHI BanI
BanII BbsI BbvCI BbvI BcgI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspHI BspMI BsrDI BsrI BssKI
BstAPI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KpnI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RcaI RsaI SapI Sau3AI Sau96I
SbfI ScrFI SfaNI SfcI SmlI
Sse8387I StuI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII