(Linear) (Six-base) MAPSORT of: KIEE5016.seq Check: 4415 from: 1 to: 1002 KIEE5016.orf With 182 enzymes: SgfI * December 5, 2014 12:57 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 599 1002 Size: 599 403 AleI CACnn'nnGTG Cuts at: 0 145 953 1002 Size: 145 808 49 Fragments arranged by size: 808 145 49 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 163 1002 Size: 163 839 AlwNI CAG_nnn'CTG Cuts at: 0 297 369 1002 Size: 297 72 633 Fragments arranged by size: 633 297 72 ApaI G_GGCC'C Cuts at: 0 10 78 1002 Size: 10 68 924 Fragments arranged by size: 924 68 10 ApaLI G'TGCA_C Cuts at: 0 138 1002 Size: 138 864 AscI GG'CGCG_CC Cuts at: 0 466 1002 Size: 466 536 AvaI C'yCGr_G Cuts at: 0 102 130 198 802 972 1002 Size: 102 28 68 604 170 30 Fragments arranged by size: 604 170 102 68 30 28 BanI G'GyrC_C Cuts at: 0 115 265 402 653 1002 Size: 115 150 137 251 349 Fragments arranged by size: 349 251 150 137 115 BanII G_rGCy'C Cuts at: 0 10 78 479 853 860 982 1002 Size: 10 68 401 374 7 122 20 Fragments arranged by size: 401 374 122 68 20 10 7 BbeI G_GCGC'C Cuts at: 0 119 1002 Size: 119 883 BlpI GC'TnA_GC Cuts at: 0 715 1002 Size: 715 287 Bme1580I G_kGCm'C Cuts at: 0 10 78 142 197 1002 Size: 10 68 64 55 805 Fragments arranged by size: 805 68 64 55 10 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 493 1002 Size: 493 509 Bpu10I CC'TnA_GC Cuts at: 0 205 376 1002 Size: 205 171 626 Fragments arranged by size: 626 205 171 BsaI GGTCTCn'nnnn_ Cuts at: 0 909 1002 Size: 909 93 BsaAI yAC'GTr Cuts at: 0 143 1002 Size: 143 859 BsaHI Gr'CG_yC Cuts at: 0 116 705 740 1002 Size: 116 589 35 262 Fragments arranged by size: 589 262 116 35 BseYI C'CCAG_C Cuts at: 0 558 846 1002 Size: 558 288 156 Fragments arranged by size: 558 288 156 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 304 571 1002 Size: 304 267 431 Fragments arranged by size: 431 304 267 BsiEI CG_ry'CG Cuts at: 0 31 1002 Size: 31 971 BsiHKAI G_wGCw'C Cuts at: 0 142 689 1002 Size: 142 547 313 Fragments arranged by size: 547 313 142 BsmBI CGTCTCn'nnnn_ Cuts at: 0 673 1002 Size: 673 329 Bsp1286I G_dGCh'C Cuts at: 0 10 78 142 197 479 689 853 860 Size: 10 68 64 55 282 210 164 7 Cuts at: 860 982 1002 Size: 122 20 Fragments arranged by size: 282 210 164 122 68 64 55 20 10 7 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 311 599 1002 Size: 311 288 403 Fragments arranged by size: 403 311 288 BsrBI CCG'CTC Cuts at: 0 280 574 633 768 1002 Size: 280 294 59 135 234 Fragments arranged by size: 294 280 234 135 59 BsrDI GCAATG_nn' Cuts at: 0 753 1002 Size: 753 249 BsrFI r'CCGG_y Cuts at: 0 451 458 650 807 1002 Size: 451 7 192 157 195 Fragments arranged by size: 451 195 192 157 7 BssHII G'CGCG_C Cuts at: 0 219 221 284 356 426 428 466 491 Size: 219 2 63 72 70 2 38 25 Cuts at: 491 620 758 874 1002 Size: 129 138 116 128 Fragments arranged by size: 219 138 129 128 116 72 70 63 38 25 2 2 BstAPI GCAn_nnn'nTGC Cuts at: 0 327 660 1002 Size: 327 333 342 Fragments arranged by size: 342 333 327 BstXI CCAn_nnnn'nTGG Cuts at: 0 390 1002 Size: 390 612 BtgI C'CryG_G Cuts at: 0 89 479 788 948 965 1002 Size: 89 390 309 160 17 37 Fragments arranged by size: 390 309 160 89 37 17 DraIII CAC_nnn'GTG Cuts at: 0 327 1002 Size: 327 675 EaeI y'GGCC_r Cuts at: 0 28 86 161 449 697 785 1002 Size: 28 58 75 288 248 88 217 Fragments arranged by size: 288 248 217 88 75 58 28 EagI C'GGCC_G Cuts at: 0 28 1002 Size: 28 974 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 895 1002 Size: 895 107 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 493 1002 Size: 493 509 EcoO109I rG'GnC_Cy Cuts at: 0 6 7 107 348 780 1002 Size: 6 1 100 241 432 222 Fragments arranged by size: 432 241 222 100 6 1 FseI GG_CCGG'CC Cuts at: 0 811 1002 Size: 811 191 FspI TGC'GCA Cuts at: 0 436 1002 Size: 436 566 HaeII r_GCGC'y Cuts at: 0 119 297 383 487 847 1002 Size: 119 178 86 104 360 155 Fragments arranged by size: 360 178 155 119 104 86 KasI G'GCGC_C Cuts at: 0 115 1002 Size: 115 887 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 761 1002 Size: 761 241 MscI TGG'CCA Cuts at: 0 163 1002 Size: 163 839 MslI CAynn'nnrTG Cuts at: 0 145 953 1002 Size: 145 808 49 Fragments arranged by size: 808 145 49 MspA1I CmG'CkG Cuts at: 0 33 167 366 390 423 481 501 660 Size: 33 134 199 24 33 58 20 159 Cuts at: 660 967 988 1002 Size: 307 21 14 Fragments arranged by size: 307 199 159 134 58 33 33 24 21 20 14 NaeI GCC'GGC Cuts at: 0 453 652 809 1002 Size: 453 199 157 193 Fragments arranged by size: 453 199 193 157 NarI GG'CG_CC Cuts at: 0 116 1002 Size: 116 886 NgoMIV G'CCGG_C Cuts at: 0 451 650 807 1002 Size: 451 199 157 195 Fragments arranged by size: 451 199 195 157 NspI r_CATG'y Cuts at: 0 334 1002 Size: 334 668 PasI CC'CwG_GG Cuts at: 0 111 1002 Size: 111 891 PmlI CAC'GTG Cuts at: 0 143 1002 Size: 143 859 PpuMI rG'GwC_Cy Cuts at: 0 107 780 1002 Size: 107 673 222 Fragments arranged by size: 673 222 107 PspOMI G'GGCC_C Cuts at: 0 6 74 1002 Size: 6 68 928 Fragments arranged by size: 928 68 6 PstI C_TGCA'G Cuts at: 0 365 524 1002 Size: 365 159 478 Fragments arranged by size: 478 365 159 PvuII CAG'CTG Cuts at: 0 167 366 390 1002 Size: 167 199 24 612 Fragments arranged by size: 612 199 167 24 SacII CC_GC'GG Cuts at: 0 482 968 1002 Size: 482 486 34 Fragments arranged by size: 486 482 34 SanDI GG'GwC_CC Cuts at: 0 107 1002 Size: 107 895 SbfI CC_TGCA'GG Cuts at: 0 524 1002 Size: 524 478 SfcI C'TryA_G Cuts at: 0 289 361 520 1002 Size: 289 72 159 482 Fragments arranged by size: 482 289 159 72 SfoI GGC'GCC Cuts at: 0 117 1002 Size: 117 885 SmaI CCC'GGG Cuts at: 0 200 974 1002 Size: 200 774 28 Fragments arranged by size: 774 200 28 StuI AGG'CCT Cuts at: 0 158 1002 Size: 158 844 StyI C'CwwG_G Cuts at: 0 61 604 1002 Size: 61 543 398 Fragments arranged by size: 543 398 61 XbaI T'CTAG_A Cuts at: 0 576 1002 Size: 576 426 XmaI C'CCGG_G Cuts at: 0 198 972 1002 Size: 198 774 30 Fragments arranged by size: 774 198 30 Enzymes that do cut: AarI AleI AlfI AlwNI ApaI ApaLI AscI AvaI BanI BanII BbeI BlpI Bme1580I BpmI Bpu10I BsaI BsaAI BsaHI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII BstAPI BstXI BtgI DraIII EaeI EagI EciI Eco57MI EcoO109I FseI FspI HaeII KasI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI PmlI PpuMI PspOMI PstI PvuII SacII SanDI SbfI SfcI SfoI SmaI StuI StyI XbaI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AloI ApoI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BmgBI BmrI BmtI BplI BpuEI BsaBI BsaWI BsaXI BseRI BsiWI BsmI BspEI BspHI BsrGI BssSI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DrdI EarI EcoICRI EcoNI EcoRI EcoRV FalI FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI NcoI NdeI NheI NotI NruI NsiI PacI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI SapI ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI SwaI TaqII TatI TstI Tth111I XcmI XhoI XmnI ZraI