################################## # NO CUT ENZYMES ( 140 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AflIII AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AviII AvrII BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaHI BsaI BseAI BseRI BsiHKAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoT22I FauI FbaI FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI MamI MfeI MflI MluI MroI MseI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PleI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SalI SapI SbfI ScaI SfiI SfoI SfuI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I Tth111I Van91I XbaI XhoI XhoII ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AccI 112 GTCTAC GT^[AC][GT]AC AccII 27 CGCG CG^CG AcsI 43 GAATTC [GA]^AATT[CT] AgeI 730 ACCGGT A^CCGGT AocI 912 CCTCAGG CC^T[ACGT]AGG ApoI 43 GAATTC [GA]^AATT[CT] Asp700I 598 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BfaI 256 CTAG C^TAG BmyI 302 GTGCCC G[AGT]GC[ACT]^C BsaAI 379 TACGTG [CT]AC^GT[GA] BsaWI 730 ACCGGT [AT]^CCGG[AT] BsgI 576 GTGCAG GTGCAG(16/14) BsiEI 701 CGGTCG CG[GA][CT]^CG BsmBI 860 CGTCTC CGTCTC(1/5) Bsp1286I 302 GTGCCC G[AGT]GC[ACT]^C BsrBI 519 CCGCTC CCGCTC(-3/-3) BsrFI 730 ACCGGT [GA]^CCGG[CT] BsrI 587 CCAGT ACTGG(1/-1) BssSI 573 CTCGTG CACGAG(-5/-1) BstAPI 469 GCAGTGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 27 CGCG CG^CG Bsu36I 912 CCTCAGG CC^T[ACGT]AGG Cfr10I 730 ACCGGT [GA]^CCGG[CT] Eco81I 912 CCTCAGG CC^T[ACGT]AGG EcoRI 43 GAATTC G^AATTC EcoRV 550 GATATC GAT^ATC EcoT14I 362 CCATGG C^C[AT][AT]GG HaeII 147 GGCGCT [GA]GCGC^[CT] HgaI 426 GCGTC GACGC(5/10) HinfI 932 GATTC G^A[ACGT]TC KspI 26 CCGCGG CCGC^GG MaeI 256 CTAG C^TAG MaeII 380 ACGT A^CGT MaeIII 581 GTGAC ^GT[ACGT]AC MspA1I 26 CCGCGG C[AC]G^C[GT]G MvnI 27 CGCG CG^CG NcoI 362 CCATGG C^CATGG NspI 692 ACATGC [GA]CATG^[CT] PinAI 730 ACCGGT A^CCGGT PshAI 583 GACACCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacII 26 CCGCGG CCGC^GG SexAI 116 ACCTGGT A^CC[AT]GGT SfcI 879 CTACAG C^T[GA][CT]AG SgrAI 729 CACCGGTG C[GA]^CCGG[CT]G SmaI 20 CCCGGG CCC^GGG StuI 456 AGGCCT AGG^CCT StyI 362 CCATGG C^C[AT][AT]GG TaqI 704 TCGA T^CGA TfiI 932 GATTC G^A[AT]TC Tsp45I 581 GTGAC ^GT[GC]AC Tsp509I 44 AATT ^AATT TthHB8I 704 TCGA T^CGA XmaCI 20 CCCGGG C^CCGGG XmaI 20 CCCGGG C^CCGGG XmnI 598 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 8 enzymes) # ################################### AlwI 156 GATCC GGATC(4/5) 687 GATCC AvaI 20 CCCGGG C^[CT]CG[GA]G 275 CCCGAG BsmAI 590 GTCTC GTCTC(1/5) 861 GTCTC BsoBI 20 CCCGGG C^[CT]CG[GA]G 275 CCCGAG EarI 91 CTCTTC CTCTTC(1/4) 607 CTCTTC Ksp632I 91 CTCTTC CTCTTC(1/4) 607 CTCTTC PpuMI 74 GGGACCT [GA]G^G[AT]CC[CT] 952 GGGTCCC XcmI 248 CCAAGATGCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 803 CCAGCTATGCCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 55 enzymes) # ######################################### AciI AfaI AluI AvaII BalI BbvI BcnI BpmI BsaJI BsiYI BslI BsmFI BspMI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MboI MboII MluNI MnlI MscI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI TseI TspRI