(Circular) (Six-base) MAPSORT of: pFN21AE6064.seq Check: 1320 from: 1 to: 5665 pFN21AE6064 5665 bp With 182 enzymes: SgfI * December 8, 2014 13:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3475 278 Size: 53 83 186 936 1939 2468 Fragments arranged by size: 2468 1939 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2118 3008 1937 Size: 181 890 4594 Fragments arranged by size: 4594 890 181 Acc65I G'GTAC_C Cuts at: 2986 2986 Size: 5665 AclI AA'CG_TT Cuts at: 1563 3792 4165 1563 Size: 2229 373 3063 Fragments arranged by size: 3063 2229 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3728 5112 5380 1632 Size: 2096 1384 268 1917 Fragments arranged by size: 2096 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5468 AflIII A'CryG_T Cuts at: 1203 4564 5541 1203 Size: 3361 977 1327 Fragments arranged by size: 3361 1327 977 AgeI A'CCGG_T Cuts at: 2735 4496 2735 Size: 1761 3904 AhdI GACnn_n'nnGTC Cuts at: 4394 4394 Size: 5665 AleI CACnn'nnGTG Cuts at: 1166 2756 1166 Size: 1590 4075 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2171 1184 Size: 987 4678 AlwNI CAG_nnn'CTG Cuts at: 3408 4980 3408 Size: 1572 4093 ApaLI G'TGCA_C Cuts at: 3722 4878 3722 Size: 1156 4509 ApoI r'AATT_y Cuts at: 1493 2048 2974 3200 3352 5461 1493 Size: 555 926 226 152 2109 1697 Fragments arranged by size: 2109 1697 926 555 226 152 AseI AT'TA_AT Cuts at: 160 1032 4219 160 Size: 872 3187 1606 Fragments arranged by size: 3187 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5665 AvaI C'yCGr_G Cuts at: 1870 1943 2025 2280 2990 1870 Size: 73 82 255 710 4545 Fragments arranged by size: 4545 710 255 82 73 BamHI G'GATC_C Cuts at: 2995 3362 2995 Size: 367 5298 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2986 4441 618 Size: 325 217 627 1199 1455 1842 Fragments arranged by size: 1842 1455 1199 627 325 217 BanII G_rGCy'C Cuts at: 1391 2984 1391 Size: 1593 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4694 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3856 3890 1903 Size: 34 1919 34 3678 Fragments arranged by size: 3678 1919 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3557 4767 3557 Size: 1210 4455 BclI T'GATC_A Cuts at: 1548 1548 Size: 5665 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4276 136 Size: 107 122 71 631 605 2604 1525 Fragments arranged by size: 2604 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5660 5660 Size: 5665 BlpI GC'TnA_GC Cuts at: 3082 3082 Size: 5665 Bme1580I G_kGCm'C Cuts at: 1790 2311 3726 4882 1790 Size: 521 1415 1156 2573 Fragments arranged by size: 2573 1415 1156 521 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5665 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3396 4354 442 Size: 416 53 83 399 24 1979 958 1753 Fragments arranged by size: 1979 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5665 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2297 2791 2950 4325 1239 Size: 1058 494 159 1375 2579 Fragments arranged by size: 2579 1375 1058 494 159 Bpu10I CC'TnA_GC Cuts at: 2513 2513 Size: 5665 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3790 4655 4953 3790 Size: 865 298 4502 Fragments arranged by size: 4502 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4328 882 Size: 617 2829 2219 Fragments arranged by size: 2829 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2386 3456 493 Size: 711 1182 1070 2702 Fragments arranged by size: 2702 1182 1070 711 BsaBI GATnn'nnATC Cuts at: 5659 5659 Size: 5665 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3472 3854 4489 275 Size: 53 83 186 936 1939 382 635 1451 Fragments arranged by size: 1939 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2735 4098 4496 4770 4917 5328 2735 Size: 1363 398 274 147 411 3072 Fragments arranged by size: 3072 1363 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5440 Fragments arranged by size: 5440 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5665 BseYI C'CCAG_C Cuts at: 2076 2134 2449 2681 2801 4868 5364 2076 Size: 58 315 232 120 2067 496 2377 Fragments arranged by size: 2377 2067 496 315 232 120 58 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2602 1163 Size: 573 866 4226 Fragments arranged by size: 4226 866 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2709 3115 3876 4904 1540 Size: 275 186 708 406 761 1028 2301 Fragments arranged by size: 2301 1028 761 708 406 275 186 BsiHKAI G_wGCw'C Cuts at: 3726 3811 4882 3726 Size: 85 1071 4509 Fragments arranged by size: 4509 1071 85 BsmI GAATG_Cn' Cuts at: 3176 3269 3176 Size: 93 5572 BsmBI CGTCTCn'nnnn_ Cuts at: 2871 2871 Size: 5665 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2311 2984 3726 3811 4882 1391 Size: 399 521 673 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 673 521 399 85 BspEI T'CCGG_A Cuts at: 5328 5328 Size: 5665 BspHI T'CATG_A Cuts at: 3554 5284 3554 Size: 1730 3935 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2091 2114 2277 2321 2802 3020 844 Size: 1247 23 163 44 481 218 3489 Fragments arranged by size: 3489 1247 481 218 163 44 23 BsrBI CCG'CTC Cuts at: 1394 1823 2526 3552 1394 Size: 429 703 1026 3507 Fragments arranged by size: 3507 1026 703 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4160 4334 65 Size: 1183 2912 174 1396 Fragments arranged by size: 2912 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2735 4309 4496 1700 Size: 110 925 1574 187 2869 Fragments arranged by size: 2869 1574 925 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5665 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5665 BssSI C'ACGA_G Cuts at: 2578 3725 4737 2578 Size: 1147 1012 3506 Fragments arranged by size: 3506 1147 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2480 794 Size: 1686 3979 BstBI TT'CG_AA Cuts at: 4469 4469 Size: 5665 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5665 BstYI r'GATC_y Cuts at: 1972 2995 3362 3747 3764 5216 5302 5314 5660 Size: 1023 367 385 17 1452 86 12 346 Cuts at: 5660 1972 Size: 1977 Fragments arranged by size: 1977 1452 1023 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2918 2918 Size: 5665 BtgI C'CryG_G Cuts at: 513 1065 2031 2367 2643 2757 513 Size: 552 966 336 276 114 3421 Fragments arranged by size: 3421 966 552 336 276 114 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2662 487 Size: 683 56 1436 3490 Fragments arranged by size: 3490 1436 683 56 BtsI GCAGTG_nn' Cuts at: 2481 2564 2590 3181 3975 3995 2481 Size: 83 26 591 794 20 4151 Fragments arranged by size: 4151 794 591 83 26 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5665 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5630 DraI TTT'AAA Cuts at: 2969 2969 Size: 5665 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4672 809 Size: 507 3356 1802 Fragments arranged by size: 3356 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2205 2388 2571 Size: 54 1425 50 275 18 375 183 183 Cuts at: 2571 2760 3112 4001 5630 8 Size: 189 352 889 1629 43 Fragments arranged by size: 1629 1425 889 375 352 275 189 183 183 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3112 1537 Size: 275 1300 4090 Fragments arranged by size: 4090 1300 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2102 2618 3595 1353 Size: 749 516 977 3423 Fragments arranged by size: 3423 977 749 516 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2230 2498 4252 4626 4772 387 Size: 1064 138 641 268 1754 374 146 1280 Fragments arranged by size: 1754 1280 1064 641 374 268 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2297 2791 2950 3728 4325 5112 5380 Size: 393 665 494 159 778 597 787 268 Cuts at: 5380 1239 Size: 1524 Fragments arranged by size: 1524 787 778 665 597 494 393 268 159 EcoO109I rG'GnC_Cy Cuts at: 2080 2914 2958 2080 Size: 834 44 4787 Fragments arranged by size: 4787 834 44 EcoRI G'AATT_C Cuts at: 2048 2974 3352 2048 Size: 926 378 4361 Fragments arranged by size: 4361 926 378 EcoRV GAT'ATC Cuts at: 2557 2557 Size: 5665 FspI TGC'GCA Cuts at: 4171 4171 Size: 5665 HaeII r_GCGC'y Cuts at: 1678 2156 4812 1678 Size: 478 2656 2531 Fragments arranged by size: 2656 2531 478 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2274 2306 4306 4338 4380 Size: 32 135 32 702 32 2000 32 42 Cuts at: 4380 4412 1373 Size: 32 2626 Fragments arranged by size: 2626 2000 702 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3009 3255 669 Size: 988 281 1071 246 3079 Fragments arranged by size: 3079 1071 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5665 HpaI GTT'AAC Cuts at: 3255 3255 Size: 5665 KpnI G_GTAC'C Cuts at: 2990 2990 Size: 5665 MfeI C'AATT_G Cuts at: 3264 3264 Size: 5665 MluI A'CGCG_T Cuts at: 5541 5541 Size: 5665 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4779 4963 1305 Size: 3474 184 2007 Fragments arranged by size: 3474 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2207 2390 2573 2762 10 Size: 54 1425 343 375 183 183 189 2913 Fragments arranged by size: 2913 1425 375 343 189 183 183 54 MslI CAynn'nnrTG Cuts at: 518 1166 2147 2189 2756 2786 3623 3982 4141 Size: 648 981 42 567 30 837 359 159 Cuts at: 4141 518 Size: 2042 Fragments arranged by size: 2042 981 837 648 567 359 159 42 30 MspA1I CmG'CkG Cuts at: 1606 2033 3081 3758 4906 5151 1606 Size: 427 1048 677 1148 245 2120 Fragments arranged by size: 2120 1148 1048 677 427 245 NcoI C'CATG_G Cuts at: 513 1065 2367 513 Size: 552 1302 3811 Fragments arranged by size: 3811 1302 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5665 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5665 NotI GC'GGCC_GC Cuts at: 3112 3112 Size: 5665 NspI r_CATG'y Cuts at: 1132 1229 2701 3023 4568 1132 Size: 97 1472 322 1545 2229 Fragments arranged by size: 2229 1545 1472 322 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5665 PciI A'CATG_T Cuts at: 4564 4564 Size: 5665 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5665 PfoI T'CCnGG_A Cuts at: 2528 2528 Size: 5665 PmeI GTTT'AAAC Cuts at: 2969 2969 Size: 5665 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2595 2627 3734 3766 5247 5279 2595 Size: 32 1107 32 1481 32 2981 Fragments arranged by size: 2981 1481 1107 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2080 2958 2080 Size: 878 4787 PshAI GACnn'nnGTC Cuts at: 1613 2592 1613 Size: 979 4686 PsiI TTA'TAA Cuts at: 3235 3235 Size: 5665 PstI C_TGCA'G Cuts at: 830 3017 830 Size: 2187 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5665 SacII CC_GC'GG Cuts at: 2034 2034 Size: 5665 SalI G'TCGA_C Cuts at: 1936 3007 1936 Size: 1071 4594 SanDI GG'GwC_CC Cuts at: 2958 2958 Size: 5665 SbfI CC_TGCA'GG Cuts at: 3017 3017 Size: 5665 ScaI AGT'ACT Cuts at: 3913 5625 3913 Size: 1712 3953 SexAI A'CCwGG_T Cuts at: 2121 2121 Size: 5665 SfcI C'TryA_G Cuts at: 826 1045 2884 3013 4148 4829 5020 826 Size: 219 1839 129 1135 681 191 1471 Fragments arranged by size: 1839 1471 1135 681 219 191 129 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5665 SgrAI Cr'CCGG_yG Cuts at: 1700 2735 1700 Size: 1035 4630 SmaI CCC'GGG Cuts at: 1872 2027 2992 1872 Size: 155 965 4545 Fragments arranged by size: 4545 965 155 SmlI C'TyrA_G Cuts at: 820 1017 1943 3769 4670 4932 820 Size: 197 926 1826 901 262 1553 Fragments arranged by size: 1826 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3456 493 Size: 2963 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5665 SphI G_CATG'C Cuts at: 1132 3023 1132 Size: 1891 3774 SspI AAT'ATT Cuts at: 5 52 3589 5 Size: 47 3537 2081 Fragments arranged by size: 3537 2081 47 StuI AGG'CCT Cuts at: 1517 2463 1517 Size: 946 4719 StyI C'CwwG_G Cuts at: 513 1065 2367 3104 513 Size: 552 1302 737 3074 Fragments arranged by size: 3074 1302 737 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2456 2695 3692 3709 3862 4047 4492 Size: 1255 876 239 997 17 153 185 445 Cuts at: 4492 325 Size: 1498 Fragments arranged by size: 1498 1255 997 876 445 239 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2193 3911 5623 Size: 274 80 33 51 1443 216 1718 1712 Cuts at: 5623 96 Size: 138 Fragments arranged by size: 1718 1712 1443 274 216 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5625 5657 5625 Size: 32 5633 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5665 XbaI T'CTAG_A Cuts at: 3001 3001 Size: 5665 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2260 2815 2260 Size: 555 5110 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5665 XmaI C'CCGG_G Cuts at: 1870 2025 2990 1870 Size: 155 965 4545 Fragments arranged by size: 4545 965 155 XmnI GAAnn'nnTTC Cuts at: 2607 3794 2607 Size: 1187 4478 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3473 276 Size: 53 83 186 936 1939 2468 Fragments arranged by size: 2468 1939 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SapI SfiI SfoI SrfI SwaI