################################## # NO CUT ENZYMES ( 161 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsgI BsiWI BsmBI BsmFI BsmI BsoBI BspDI BspEI BspHI BspLU11I BsrBI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I CelII CfoI ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MaeII MamI MfeI MflI MluI MroI MspA1I MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AccII 478 CGCG CG^CG AlwI 297 GATCC GGATC(4/5) AvaII 747 GGTCC G^G[AT]CC BcnI 816 CCGGG CC^[GC]GG BglI 349 GCCTCTATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 582 CTCCAG CTGGAG(16/14) BsaI 607 GAGACC GGTCTC(1/5) BsiEI 363 CGACCG CG[GA][CT]^CG Bsp1407I 833 TGTACA T^GTACA BsrDI 421 CATTGC GCAATG(2/0) BsrFI 365 ACCGGC [GA]^CCGG[CT] BsrGI 833 TGTACA T^GTACA BstUI 478 CGCG CG^CG BstXI 584 CCAGCCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 365 ACCGGC [GA]^CCGG[CT] DpnII 297 GATC ^GATC DsaI 415 CCATGG C^C[GA][CT]GG EarI 54 CTCTTC CTCTTC(1/4) EcoRV 211 GATATC GAT^ATC EcoT14I 415 CCATGG C^C[AT][AT]GG HinfI 683 GAATC G^A[ACGT]TC Ksp632I 54 CTCTTC CTCTTC(1/4) MboI 297 GATC ^GATC MvnI 478 CGCG CG^CG NciI 816 CCGGG CC^[GC]GG NcoI 415 CCATGG C^CATGG NdeII 297 GATC ^GATC Sau3AI 297 GATC ^GATC SfiI 348 GGCCTCTATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SspBI 833 TGTACA T^GTACA StyI 415 CCATGG C^C[AT][AT]GG TaqI 362 TCGA T^CGA TfiI 683 GAATC G^A[AT]TC TthHB8I 362 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AciI 477 CCGC CCGC(-3/-1) 118 GCGG AcsI 252 GAATTT [GA]^AATT[CT] 923 GAATTT AocI 29 CCTCAGG CC^T[ACGT]AGG 876 CCTGAGG ApoI 252 GAATTT [GA]^AATT[CT] 923 GAATTT Asp700I 708 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 858 GAACCCTTTC AspHI 130 GTGCTC G[AT]GC[AT]^C 676 GTGCTC BmyI 130 GTGCTC G[AGT]GC[ACT]^C 676 GTGCTC BsiHKAI 130 GTGCTC G[AT]GC[AT]^C 676 GTGCTC Bsp1286I 130 GTGCTC G[AGT]GC[ACT]^C 676 GTGCTC BspMI 341 ACCTGC ACCTGC(4/8) 656 ACCTGC Bsu36I 29 CCTCAGG CC^T[ACGT]AGG 876 CCTGAGG Cac8I 345 GCTGGC GC[ACGT]^[ACGT]GC 479 GCGTGC Eco81I 29 CCTCAGG CC^T[ACGT]AGG 876 CCTGAGG HapII 366 CCGG C^CGG 816 CCGG HpaII 366 CCGG C^CGG 816 CCGG HphI 249 GGTGA GGTGA(8/7) 597 GGTGA MseI 524 TTAA T^TAA 913 TTAA MspI 366 CCGG C^CGG 816 CCGG NlaIV 164 GGCTCC GG[ACGT]^[ACGT]CC 727 GGCTCC PflMI 588 CCAGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 698 CCAGGAAGTGG PstI 70 CTGCAG CTGCA^G 692 CTGCAG SfaNI 243 GATGC GCATC(5/9) 452 GCATC StuI 710 AGGCCT AGG^CCT 784 AGGCCT Tru9I 524 TTAA T^TAA 913 TTAA Van91I 588 CCAGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 698 CCAGGAAGTGG XcmI 359 CCATCGACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 584 CCAGCCAGATGGTGG XmnI 708 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 858 GAACCCTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 36 enzymes) # ######################################### AfaI AluI BalI BbvI BsaJI BsiYI BslI BsmAI BssKI BstF5I BstNI Cfr13I DdeI EaeI EcoRII Fnu4HI FokI HaeIII ItaI MaeIII MboII MluNI MnlI MscI MslI MvaI MwoI NlaIII RsaI Sau96I ScrFI SfcI TseI Tsp45I Tsp509I TspRI