################################## # NO CUT ENZYMES ( 178 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BanI BanII BbeI BbrPI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaHI BsaI BsaJI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsoBI Bsp1286I BspDI BspEI BspHI BsrBI BsrDI BsrFI BsrI BssHII Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT14I EcoT22I FauI FbaI FokI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HindIII HpaI HpaII KasI KpnI Ksp632I KspI MaeII MfeI MflI MluI MroI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AfaI 506 GTAC GT^AC AflIII 474 ACATGT A^C[GA][CT]GT AlwI 154 GATCC GGATC(4/5) Asp700I 31 GAATTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BbsI 64 GAAGAC GAAGAC(2/6) BbvCI 686 CCTCAGC CCTCAGC(-5/-2) BfaI 433 CTAG C^TAG BpuAI 64 GAAGAC GAAGAC(2/6) BsaBI 331 GATAGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmAI 924 GTCTC GTCTC(1/5) BsmI 436 GCATTC GAATGC(1/-1) Bsp1407I 505 TGTACA T^GTACA BspLU11I 474 ACATGT A^CATGT BspMI 718 ACCTGC ACCTGC(4/8) BsrGI 505 TGTACA T^GTACA BssSI 244 CTCGTG CACGAG(-5/-1) Cac8I 609 GCTGGC GC[ACGT]^[ACGT]GC DpnII 154 GATC ^GATC EcoRI 659 GAATTC G^AATTC MaeI 433 CTAG C^TAG MamI 331 GATAGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboI 154 GATC ^GATC MwoI 346 GCGGCTATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NdeII 154 GATC ^GATC NspI 474 ACATGT [GA]CATG^[CT] PstI 720 CTGCAG CTGCA^G PvuII 692 CAGCTG CAG^CTG RsaI 506 GTAC GT^AC Sau3AI 154 GATC ^GATC SexAI 104 ACCTGGT A^CC[AT]GGT SmlI 676 CTCAAG C^T[CT][GA]AG SspBI 505 TGTACA T^GTACA TaqI 159 TCGA T^CGA TthHB8I 159 TCGA T^CGA XmnI 31 GAATTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcsI 31 GAATTT [GA]^AATT[CT] 659 GAATTC AlwNI 414 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG 689 CAGCAGCTG ApoI 31 GAATTT [GA]^AATT[CT] 659 GAATTC AvaII 807 GGACC G^G[AT]CC 967 GGACC BalI 353 TGGCCA TGG^CCA 593 TGGCCA BseRI 51 CTCCTC GAGGAG(10/8) 567 CTCCTC BsmFI 45 GGGAC GGGAC(10/14) 232 GTCCC Cfr13I 807 GGACC G^G[ACGT]CC 967 GGACC EaeI 353 TGGCCA [CT]^GGCC[GA] 593 TGGCCA HincII 541 GTCAAC GT[CT]^[GA]AC 837 GTTGAC HindII 541 GTCAAC GT[CT]^[GA]AC 837 GTTGAC MboII 64 GAAGA GAAGA(8/7) 308 TCTTC MluNI 353 TGGCCA TGG^CCA 593 TGGCCA MscI 353 TGGCCA TGG^CCA 593 TGGCCA MseI 871 TTAA T^TAA 920 TTAA MspA1I 363 CCGCTG C[AC]G^C[GT]G 692 CAGCTG NlaIV 624 GGCTCC GG[ACGT]^[ACGT]CC 967 GGACCC PleI 23 GACTC GAGTC(4/5) 47 GACTC Sau96I 807 GGACC G^G[ACGT]CC 967 GGACC SfcI 720 CTGCAG C^T[GA][CT]AG 864 CTACAG Tru9I 871 TTAA T^TAA 920 TTAA Tsp45I 109 GTCAC ^GT[GC]AC 397 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 23 enzymes) # ######################################### AciI AluI BbvI BssKI BstNI DdeI EcoRII Fnu4HI HaeIII HinfI HphI ItaI MaeIII MnlI MslI MvaI NlaIII ScrFI SfaNI TfiI TseI Tsp509I TspRI