(Circular) (Six-base) MAPSORT of: pFN21AE5971.seq Check: 5188 from: 1 to: 5641 pFN21AE5971 5641 bp With 182 enzymes: SgfI * December 8, 2014 13:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2175 2546 2732 2175 Size: 371 186 5084 Fragments arranged by size: 5084 371 186 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3451 278 Size: 53 83 186 936 1915 2468 Fragments arranged by size: 2468 1915 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2984 1937 Size: 1047 4594 Acc65I G'GTAC_C Cuts at: 2397 2962 2397 Size: 565 5076 AclI AA'CG_TT Cuts at: 1563 3768 4141 1563 Size: 2205 373 3063 Fragments arranged by size: 3063 2205 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2579 3704 5088 5356 1632 Size: 947 1125 1384 268 1917 Fragments arranged by size: 1917 1384 1125 947 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5444 AflIII A'CryG_T Cuts at: 1203 2577 4540 5517 1203 Size: 1374 1963 977 1327 Fragments arranged by size: 1963 1374 1327 977 AgeI A'CCGG_T Cuts at: 4472 4472 Size: 5641 AhdI GACnn_n'nnGTC Cuts at: 4370 4370 Size: 5641 AleI CACnn'nnGTG Cuts at: 1166 2177 2234 1166 Size: 1011 57 4573 Fragments arranged by size: 4573 1011 57 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2662 1184 Size: 1478 4163 AlwNI CAG_nnn'CTG Cuts at: 2706 3384 4956 2706 Size: 678 1572 3391 Fragments arranged by size: 3391 1572 678 ApaLI G'TGCA_C Cuts at: 3698 4854 3698 Size: 1156 4485 ApoI r'AATT_y Cuts at: 1493 2950 3176 3328 5437 1493 Size: 1457 226 152 2109 1697 Fragments arranged by size: 2109 1697 1457 226 152 AseI AT'TA_AT Cuts at: 160 1032 4195 160 Size: 872 3163 1606 Fragments arranged by size: 3163 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5641 AvaI C'yCGr_G Cuts at: 1870 1943 2966 1870 Size: 73 1023 4545 Fragments arranged by size: 4545 1023 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2381 2414 2381 Size: 33 5608 BamHI G'GATC_C Cuts at: 2971 3338 2971 Size: 367 5274 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2397 2686 2762 2962 4417 Size: 325 217 627 610 289 76 200 1455 Cuts at: 4417 618 Size: 1842 Fragments arranged by size: 1842 1455 627 610 325 289 217 200 76 BanII G_rGCy'C Cuts at: 1391 2330 2612 2960 1391 Size: 939 282 348 4072 Fragments arranged by size: 4072 939 348 282 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2279 2598 2815 928 Size: 971 380 319 217 3754 Fragments arranged by size: 3754 971 380 319 217 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3832 3866 1903 Size: 34 1895 34 3678 Fragments arranged by size: 3678 1895 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3533 4743 3533 Size: 1210 4431 BclI T'GATC_A Cuts at: 1548 1548 Size: 5641 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2141 2357 4252 Size: 107 122 71 631 605 469 216 1895 Cuts at: 4252 136 Size: 1525 Fragments arranged by size: 1895 1525 631 605 469 216 122 107 71 BglII A'GATC_T Cuts at: 5636 5636 Size: 5641 BlpI GC'TnA_GC Cuts at: 2098 3058 2098 Size: 960 4681 Bme1580I G_kGCm'C Cuts at: 1790 2691 3702 4858 1790 Size: 901 1011 1156 2573 Fragments arranged by size: 2573 1156 1011 901 BmgBI CAC'GTC Cuts at: 1318 2580 1318 Size: 1262 4379 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3372 4330 442 Size: 416 53 83 399 24 1955 958 1753 Fragments arranged by size: 1955 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5641 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2093 2920 4301 1239 Size: 854 827 1381 2579 Fragments arranged by size: 2579 1381 854 827 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2450 3766 4631 4929 2450 Size: 1316 865 298 3162 Fragments arranged by size: 3162 1316 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4304 882 Size: 617 2805 2219 Fragments arranged by size: 2805 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2404 2521 3432 493 Size: 711 1200 117 911 2702 Fragments arranged by size: 2702 1200 911 711 117 BsaBI GATnn'nnATC Cuts at: 2486 5635 2486 Size: 3149 2492 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3448 3830 4465 275 Size: 53 83 186 936 1915 382 635 1451 Fragments arranged by size: 1915 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4074 4472 4746 4893 5304 4074 Size: 398 274 147 411 4411 Fragments arranged by size: 4411 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5416 Fragments arranged by size: 5416 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2453 1186 Size: 1267 4374 BseYI C'CCAG_C Cuts at: 2065 4844 5340 2065 Size: 2779 496 2366 Fragments arranged by size: 2779 2366 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2153 1163 Size: 573 417 4651 Fragments arranged by size: 4651 573 417 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2697 3091 3852 4880 1540 Size: 275 186 696 394 761 1028 2301 Fragments arranged by size: 2301 1028 761 696 394 275 186 BsiHKAI G_wGCw'C Cuts at: 2099 2409 3702 3787 4858 2099 Size: 310 1293 85 1071 2882 Fragments arranged by size: 2882 1293 1071 310 85 BsmI GAATG_Cn' Cuts at: 2639 3152 3245 2639 Size: 513 93 5035 Fragments arranged by size: 5035 513 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2099 2330 2409 2612 2691 2960 3702 Size: 399 309 231 79 203 79 269 742 Cuts at: 3702 3787 4858 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 309 269 231 203 85 79 79 BspEI T'CCGG_A Cuts at: 5304 5304 Size: 5641 BspHI T'CATG_A Cuts at: 2409 3530 5260 2409 Size: 1121 1730 2790 Fragments arranged by size: 2790 1730 1121 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2175 2546 2732 2996 844 Size: 1331 371 186 264 3489 Fragments arranged by size: 3489 1331 371 264 186 BsrBI CCG'CTC Cuts at: 1394 1823 2014 3528 1394 Size: 429 191 1514 3507 Fragments arranged by size: 3507 1514 429 191 BsrDI GCAATG_nn' Cuts at: 65 1248 4136 4310 65 Size: 1183 2888 174 1396 Fragments arranged by size: 2888 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2418 4285 4472 1700 Size: 110 608 1867 187 2869 Fragments arranged by size: 2869 1867 608 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5641 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5641 BssSI C'ACGA_G Cuts at: 3701 4713 3701 Size: 1012 4629 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5641 BstBI TT'CG_AA Cuts at: 4445 4445 Size: 5641 BstEII G'GTnAC_C Cuts at: 1184 2113 1184 Size: 929 4712 BstXI CCAn_nnnn'nTGG Cuts at: 2110 2728 2110 Size: 618 5023 BstYI r'GATC_y Cuts at: 1972 2544 2971 3338 3723 3740 5192 5278 5290 Size: 572 427 367 385 17 1452 86 12 Cuts at: 5290 5636 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 572 427 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2681 2879 2681 Size: 198 5443 BtgI C'CryG_G Cuts at: 513 1065 2004 2322 2331 2841 513 Size: 552 939 318 9 510 3313 Fragments arranged by size: 3313 939 552 510 318 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4902 Fragments arranged by size: 4902 683 56 BtsI GCAGTG_nn' Cuts at: 2559 3157 3951 3971 2559 Size: 598 794 20 4229 Fragments arranged by size: 4229 794 598 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5641 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5606 DraI TTT'AAA Cuts at: 2945 2945 Size: 5641 DraIII CAC_nnn'GTG Cuts at: 2337 2521 2337 Size: 184 5457 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4648 809 Size: 507 3332 1802 Fragments arranged by size: 3332 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2349 2376 2512 Size: 54 1425 50 275 18 519 27 136 Cuts at: 2512 2670 2694 3088 3977 5606 8 Size: 158 24 394 889 1629 43 Fragments arranged by size: 1629 1425 889 519 394 275 158 136 54 50 43 27 24 18 EagI C'GGCC_G Cuts at: 1537 1812 2694 3088 1537 Size: 275 882 394 4090 Fragments arranged by size: 4090 882 394 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2090 3571 1353 Size: 737 1481 3423 Fragments arranged by size: 3423 1481 737 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4228 4602 4748 387 Size: 1064 138 2639 374 146 1280 Fragments arranged by size: 2639 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2093 2579 2920 3704 4301 5088 5356 Size: 393 461 486 341 784 597 787 268 Cuts at: 5356 1239 Size: 1524 Fragments arranged by size: 1524 787 784 597 486 461 393 341 268 EcoO109I rG'GnC_Cy Cuts at: 2452 2452 Size: 5641 EcoRI G'AATT_C Cuts at: 2950 3328 2950 Size: 378 5263 EcoRV GAT'ATC Cuts at: 2161 2161 Size: 5641 FspI TGC'GCA Cuts at: 4147 4147 Size: 5641 HaeII r_GCGC'y Cuts at: 1678 4788 1678 Size: 3110 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4282 4314 4356 4388 1373 Size: 32 135 32 2710 32 42 32 2626 Fragments arranged by size: 2710 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2985 3231 669 Size: 988 281 1047 246 3079 Fragments arranged by size: 3079 1047 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5641 HpaI GTT'AAC Cuts at: 3231 3231 Size: 5641 KpnI G_GTAC'C Cuts at: 2401 2966 2401 Size: 565 5076 MfeI C'AATT_G Cuts at: 3240 3240 Size: 5641 MluI A'CGCG_T Cuts at: 5517 5517 Size: 5641 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4755 4939 1305 Size: 3450 184 2007 Fragments arranged by size: 3450 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2351 2378 2514 2672 10 Size: 54 1425 343 519 27 136 158 2979 Fragments arranged by size: 2979 1425 519 343 158 136 54 27 MslI CAynn'nnrTG Cuts at: 518 1166 2144 2177 2234 2303 2492 2774 2852 Size: 648 978 33 57 69 189 282 78 Cuts at: 2852 3599 3958 4117 518 Size: 747 359 159 2042 Fragments arranged by size: 2042 978 747 648 359 282 189 159 78 69 57 33 MspA1I CmG'CkG Cuts at: 1606 3057 3734 4882 5127 1606 Size: 1451 677 1148 245 2120 Fragments arranged by size: 2120 1451 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2841 513 Size: 552 939 837 3313 Fragments arranged by size: 3313 939 837 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5641 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5641 NotI GC'GGCC_GC Cuts at: 3088 3088 Size: 5641 NspI r_CATG'y Cuts at: 1132 1229 2571 2999 4544 1132 Size: 97 1342 428 1545 2229 Fragments arranged by size: 2229 1545 1342 428 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5641 PciI A'CATG_T Cuts at: 4540 4540 Size: 5641 PflMI CCAn_nnn'nTGG Cuts at: 1108 2521 1108 Size: 1413 4228 PfoI T'CCnGG_A Cuts at: 2267 2267 Size: 5641 PmeI GTTT'AAAC Cuts at: 2945 2945 Size: 5641 PmlI CAC'GTG Cuts at: 2404 2521 2404 Size: 117 5524 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3710 3742 5223 5255 3710 Size: 32 1481 32 4096 Fragments arranged by size: 4096 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2452 2452 Size: 5641 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5641 PsiI TTA'TAA Cuts at: 3211 3211 Size: 5641 PstI C_TGCA'G Cuts at: 830 2993 830 Size: 2163 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5641 SalI G'TCGA_C Cuts at: 1936 2983 1936 Size: 1047 4594 SapI GCTCTTCn'nnn_ Cuts at: 2090 2090 Size: 5641 SbfI CC_TGCA'GG Cuts at: 2993 2993 Size: 5641 ScaI AGT'ACT Cuts at: 3889 5601 3889 Size: 1712 3929 SexAI A'CCwGG_T Cuts at: 2109 2109 Size: 5641 SfcI C'TryA_G Cuts at: 826 1045 2742 2872 2989 4124 4805 4996 826 Size: 219 1697 130 117 1135 681 191 1471 Fragments arranged by size: 1697 1471 1135 681 219 191 130 117 SfiI GGCCn_nnn'nGGCC Cuts at: 2357 2357 Size: 5641 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5641 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5641 SmaI CCC'GGG Cuts at: 1872 2968 1872 Size: 1096 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2465 3745 4646 4908 820 Size: 197 926 522 1280 901 262 1553 Fragments arranged by size: 1553 1280 926 901 522 262 197 SnaBI TAC'GTA Cuts at: 493 3432 493 Size: 2939 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5641 SphI G_CATG'C Cuts at: 1132 2571 2999 1132 Size: 1439 428 3774 Fragments arranged by size: 3774 1439 428 SspI AAT'ATT Cuts at: 5 52 3565 5 Size: 47 3513 2081 Fragments arranged by size: 3513 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5641 StyI C'CwwG_G Cuts at: 513 1065 2004 2841 3080 513 Size: 552 939 837 239 3074 Fragments arranged by size: 3074 939 837 552 239 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2572 3668 3685 3838 4023 4468 325 Size: 1255 992 1096 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1096 992 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2181 2778 3887 Size: 274 80 33 51 1443 204 597 1109 Cuts at: 3887 5599 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1109 597 274 204 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5601 5633 5601 Size: 32 5609 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5641 XbaI T'CTAG_A Cuts at: 2977 2977 Size: 5641 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5641 XmaI C'CCGG_G Cuts at: 1870 2966 1870 Size: 1096 4545 XmnI GAAnn'nnTTC Cuts at: 3770 3770 Size: 5641 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3449 276 Size: 53 83 186 936 1915 2468 Fragments arranged by size: 2468 1915 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SfoI SrfI SwaI XcmI